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Extent of host deletions associated with bacteriophage P2-mediated eduction.

A series of independent Escherichia coli K eductants has been isolated and tested to determine the extent of their deletions. The deletions cover the P2 prophage in location H, the his operon, a suppressor of the recBC phenotype (sbcB), the gene for gluconate-6-phosphate dehydrogenase (gnd), a locus involved in cell wall synthesis (rfb), and in some cases all or part of genes involved in methylgalactoside uptake (mglP). One end of the deletion, the P2 prophage end, appears to be the same for all eductants. The other end, however, can be located before, within, and after the mglP locus.

Isolation and characterization of phages ΦZC2 and ΦZC3 against carbapenem-resistant Acinetobacter baumannii, and efficacy of ΦZC3 on A549 cells.

BACKGROUND: Acinetobacter baumannii is an opportunistic pathogen and a major causative agent of hospital-acquired infections. This pathogen … ge-resistant mutants with ΦZC3. Whole genome sequencing and annotation did not identify markers for lysogeny or antibiotic resistance. Phylogenetic analysis classified ΦZC2 and ΦZC3 within the genera of Obolenskvirus and Friunavirus, respectively. ΦZC3 was selected for its broad host range to be evaluated for rescuing A549 cells from MDR A. baumannii infec …

A Mechanism-Based Targeted Screen To Identify Epstein-Barr Virus-Directed Antiviral Agents.

Epstein-Barr virus (EBV) is one of nine human herpesviruses that persist latently to establish permanent residence in their hosts. Periodic activation into the lytic/replicative phase allows such viruses to propagate and spread, but can also cause disease in the host. This lytic phase is also essential for EBV to cause infectious mononucleosis and cancers, including B lymphocyte-derived Burkitt lymphoma and immunocompromise-associated lymphoproliferative diseases/lymphomas as well as epithelial cell-derived nasopharyngeal cell carcinoma. In the absence of anti-EBV agents, however, therapeutic

Molecular biology of the human immunodeficiency virus type 1.

The immunodeficiency virus type 1 is a complex retrovirus. In addition to genes that specify the proteins of the virus particle and the replicative enzymes common to all retroviruses, HIV-1 specifies at least six additional proteins that regulate the virus life cycle. Two of these regulatory genes, tat and rev, specify proteins essential for replication. These proteins bind to specific sequences of newly synthesized virus RNA and profoundly affect virus protein expression. Tat and rev appear to be prototypes of novel eukaryotic regulatory proteins. These two genes may play a central role in re

Dexamethasone improves therapeutic outcomes in a preclinical bacterial epididymitis mouse model.

STUDY QUESTION: Can dexamethasone improve infertility-related cauda epididymidal tissue damage caused by bacterial epididymitis? SUMMARY AN … ected. Effectiveness of antibiotic treatment was assessed by plating of epididymal homogenates onto lysogeny broth agar plates. Overall tissue morphology and the degree and nature of tissue damage were assessed histologically. Quantitative RT-PCR was used to assess local cytokine transcript levels. Blood was drawn and serum analysed for systemic IgG and Ig …

Регулирование на основе Virobiome микробиота-гут-мозговой оси сигнализирует и нейроиммунный гомеостаз.

Ось человеческого кишечника все чаще признается как сложная двунаправленная коммуникационная система, объединяющая микробные, нейронные, эндокринные и иммунные сети, формирующие неврологическое здоровье. Хотя бактериальный вклад в этот диалог получил широкую окраску, вирусный компонент кишечной экосистемы, виром, стал важным компонентом, связанным с физиологическим регулированием принимающей стороны. В этом обзоре обобщены данные, свидетельствующие о том, что бактериофаги и эукариотические вирусы могут способствовать микробному и иммунальному гомеостазу и оказывать влияние на сигнализацию вдол

Совпадение в полном тексте источника
… Another important interaction is lysogeny, in which phages integrate their genetic material into the bacterial genome, forming prophages [ 52 ]. … of bacterial products (LPS, nucleic acids) | Associated with systemic inflammation and glial activation | Reported associations: AD, MS, ASD | Phage adsorption, endolysin | TLR4, TLR9 | MyD88–NF-κB TRIF–IRF signaling | Reported reduction of TNF-α and IL-6 in experimental colitis models | [ 59 – 61 ] Lysogeny …

Виром ДНК связан с аутоиммунными заболеваниями и COVID-19.

Аберрантная иммунная реакция на вирусные патогены способствует патогенезу, но наше понимание патологических иммунных реакций, вызванных вирусами в вироме человека, особенно в масштабе населения, остается ограниченным. Мы проанализировали наборы данных о последовательности целых генов 6,321 Японцы, включая пациентов с аутоиммунными заболеваниями (псориаз вульгарис, ревматоидный артрит (RA), системный волшебный эритематос (SLE), легочный альвеолярный протеиноз (PAP) или множественный склероз) и коронавирусное заболевание 2019 (В тыс.COVID-19) или здоровый контроль. Систематическая количественная

Совпадение в полном тексте источника
… Following immunoprecipitation at 4 °C for 1 h on a rotator, beads were washed three times with 1,500 μl of PhIP-seq wash buffer (50 mM Tris–HCl (pH 7.5), 150 mM NaCl and 0.1% Tween 20) and resuspended in lysogeny broth with antibiotics. …

Oral Presentations

Introduction: As robotic-guidance and minimally invasive approaches become more prevalent in spine surgery, there is a growing interest in h … ively for 2015. Representative bacterial cultures were harvested from wound swabs and cultivated in Lysogeny Broth. PVP-Iodine (Braunol®), Hexetidin (Isozid®), Propanol/Biphenyol (Kodan forte®) and Octenidin (Octenisept®) were evaluated. Bacterial proliferation was monitored by measuring medium turbidity at 600 nm prior to disinfection, immediately and aft …

ISEV2019 Abstract Book

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