Результаты поиска

Integrated counter current separation and omics approaches for discovery of bioactive compounds from medicinal plants.

Medicinal plants provide an important source of structurally varied bioactive metabolites with medicinal potential; however, their effective … very rates. Simultaneously, the development of metabolomics, transcriptomics, proteomics, genomics, lipidomics, and glycomics has significantly advanced the research on natural products, enabling detailed molecular profiling and systems-level biological interpretation. This review critically considers the use of CCS in combination with multi-omics technologi …

LMAN2 promotes breast cancer progression and deduces cell sensitivity to doxorubicin through stearoyl-CoA desaturase.

BACKGROUND: Dysregulated lipid metabolism and chemoresistance are key drivers of breast cancer progression. Lectin, mannose-binding 2 (LMAN2 … migration, apoptosis, and response to ADM were systematically assessed. RNA-sequencing, untargeted lipidomics, and rescue experiments identified stearoyl-CoA desaturase (SCD) as a critical downstream effector. IC50 shifts and epistasis analysis further validated the role of the LMAN2/SCD axis in chemoresistance. RESULTS: LMAN2 mRNA was elevated across all …

Ceramide-Mediated Lipotoxicity: A Metabolic Driver of Myocardial Dysfunction in Heart Failure with Preserved Ejection Fraction.

INTRODUCTION/OBJECTIVE: Heart Failure with Preserved Ejection Fraction (HFpEF) is increasingly recognized as a metabolic disorder. This revi … lidation in human HFpEF myocardium is urgently needed. Future research must prioritize human tissue lipidomics, development of multifactorial animal models, and targeted clinical trials to translate these findings into effective therapies.

Effects of dietary Chaihu-Shugan-San on egg production performance, egg quality, and hepatic lipid metabolism of late-laying hens.

This study aimed to evaluate the effects of Chaihu-Shugan-San (CSS) on egg production, egg quality, ovarian development, serum lipid levels, … cation of 1,826 and 1,828 lipid compounds in the CON and CSS groups, respectively, was indicated by lipidomics analysis. 175 differential lipids (SDLs) were upregulated and 60 SDLs were downregulated significantly. Kyoto Encyclopedia of Genes and Genomes (KEGG) topological analysis indicated that SDLs in the liver were primarily enriched in the metabolism of …

Targeted metabolomics identifies associations of plasma ceramides and their ratios with incident type 2 diabetes mellitus.

AIMS: Limited studies explored the association between ceramides and the risk of type 2 diabetes mellitus (T2DM). We aimed to investigated whether plasma ceramides and their ratios to Cer(d18:1/16:0) would be associated with T2DM in Chinese adult residents. METHODS: This investigation was designed as a matched case-control study nested within a community-based longitudinal cohort in Suzhou, China. Plasma concentrations of eight ceramides (C16-C24) were measured quantitatively in 234 cases and 468 matched controls using high-throughput targeted lipidomics. Conditional logistic regression model…

Organelle-Specific Lipid Profiles Underlie Metabolic Health in a Nutrition-Dependent Manner.

UNLABELLED: Western diet (WD), characterized by high energy density and saturated fat, promotes obesity and insulin resistance (IR), yet how dietary lipid overload remodels skeletal muscle lipids at the subcellular level remains unclear. We investigated whether WD alters lipid class distribution and fatty acid (FA) incorporation within distinct muscle organelles and whether these changes relate to metabolic health. C57BL/6J mice were fed WD or control chow for 12 weeks. Mitochondria and lipid droplets (LDs) were isolated from soleus for organelle-resolved lipidomics. WD induced obesity, dyslip…

Association between vertebral endplate defects and low back pain: an integrative omics study revealing the role of lipotoxicity-induced EGFR/COX-2 signalling.

STUDY DESIGN: Retrospective study. OBJECTIVE: To investigate the association between endplate defects and patient-reported outcomes and to … efect groups based on radiographic evidence of endplate defects. Endplate cells were analyzed using lipidomics, transcriptomics, and functional assays. Outcome measures were compared between groups. Pearson's correlation was used to assess relationships among symptoms, immune-positive cells, and lipid content. Multivariate linear analysis evaluated the contr …

Mutations in the Txnip PPXY motifs protect against myocardial infarction despite enhanced protein stability.

Thioredoxin-interacting protein (Txnip), a member of α-arrestin superfamily, is best known for inhibiting thioredoxins and glucose transport … uncovered enrichment of PPAR-α/PGC-1α-associated pathways, although metabolic assays and untargeted lipidomics did not support these findings. Instead, immediate early genes of the Fos/Jun AP-1 network were consistently downregulated after ischemic injury, and AP-1 DNA-binding activity was diminished in PPXY mutant hearts. These results identify the Txnip PP …

Multi-omics profiling of chronic immune-mediated skin diseases: SKINERGY protocol and strategic evaluation.

BACKGROUND: The Dutch flagship project Next Generation ImmunoDermatology (NGID) aims to profile five chronic immune-mediated inflammatory sk … rious biomarkers over time, including histology, metabolomics, spatial proteomics, transcriptomics, lipidomics, microbiomics, imaging biomarkers, tele-health, patient-reported outcome measures (PROMs) and clinical parameters. CONCLUSIONS: Identified biomarker profiles within SKINERGY may guide targeted treatment selection, enhance targeted therapeutic respo …

Diagnostic and therapeutic potential of serum fatty acids in hyperlipidemia: evidence from three cohorts of patients with coronary atherosclerotic heart disease.

BACKGROUND: Hyperlipidemia is a major risk factor for coronary atherosclerotic heart disease (CAHD). However, conventional lipid profiling fails to fully reflect the complexity of lipid metabolism. Furthermore, while lipidomics has identified numerous complex lipids as biomarkers, the role of their fundamental constituents-serum fatty acids-remains less explored in CAHD. In this study, we performed detailed profiling of serum fatty acid species to explore their utility in improving the identification and management of lipid disorders specifically within the CAHD population, an approach whose c…

FabI-targeting pentacyclic triterpenoid celastrol resensitizes multidrug-resistant Gram-negative bacteria to polymyxins.

BACKGROUND: Pentacyclic triterpenoids are diverse class of plant-derived bioactive scaffolds, but their potential to resensitize resistant G … lular and omics analyses, such as intracellular accumulation, genetic validation, metabolic rescue, lipidomics, and qPCR. Therapeutic efficacy was tested in murine peritonitis and cutaneous abscess models. RESULTS: CEL effectively potentiated polymyxin activity and resensitized Escherichia coli and Klebsiella pneumoniae harboring mcr-1 or pmrAB/phoPQ mutati …

Долгосрочное восстановление и нарушение оси микробиота-гут-мозгов после травматического повреждения мозга (OVERCOME-TBI): протокол перспективного клинического исследования.

ВВЕДЕНИЕ: Травматическая травма головного мозга (ТБИ) является одной из основных причин долгосрочной инвалидности во всем мире, однако патофизиологические механизмы, лежащие в основе ее хронических последствий, по-прежнему недостаточно изучены. В дополнение к стойкой нейроинфламмации, факты говорят о том, что дисрегулирование кишечного микробиота влияет на восстановление мозга. В рамках исследования OVERCOME-TBI изучаются долгосрочные функциональные, когнитивные, визуальные, биохимические и желудочно-кишечные результаты после TBI всех различий с целью выявления биомаркеров и микробиомных сигна

Совпадение в аннотации оригинала
INTRODUCTION: Traumatic brain injury (TBI) is a major cause of long-term disability worldwide, but the pathophysiological mechanisms underly … MRI, positron emission tomography (PET) with the radioligand [11C]PK11195, blood-based proteomics, lipidomics, metabolomics, faecal and intestinal microbiome profiling and colonoscopy biopsies. Moreover, 10 patients with multiple sclerosis (as a positive control group) will undergo PET imaging and sampling for blood biomarkers. Primary outcomes include neur …

Integrated multi-omics unravels solanesol's multi-organ protection mechanisms against insulin resistance in type 2 diabetic mice.

INTRODUCTION: Integrated multi-omics analysis has revolutionized the investigation of plant-derived compounds for type 2 diabetes mellitus ( … spectively. Multi-omics profiling included gut microbiota sequencing (16S rRNA), fecal metabolomics/lipidomics (UHPLC-Q LC-MS/MS), and hepatic transcriptome/proteome analysis (RNA-seq, 4-Dimensional Label-Free Quantification (4D-LFQ)). Long-/medium-/short-chain fatty acids were detected by GC-MS. RESULTS: Solanesol improved glucose tolerance, insulin sensit …

PSMA-targeted theranostic nanoplatform achieves spatiotemporally precise therapy and triggers ferroptosis in prostate cancer treatment.

BACKGROUND: Ferroptosis, an iron-dependent form of regulated cell death, is crucial for the fate of tumors such as prostate cancer (PCa) und … , the mechanisms underlying the ferroptosis effects were further explored through transcriptome and lipidomics sequencing. Intravenous administration was employed to treat subcutaneous tumors in a mouse model, and the tumor inhibitory effects, safety, and visibility on T2-weighted MRI were evaluated. RESULTS: The engineered GUL@LsiYY1@MZ nanosystem exploits …

Associations of choline intake and metabolite status with fetal growth outcomes and placental macronutrient transport in pregnancies with or without gestational diabetes mellitus.

BACKGROUND & AIMS: Gestational diabetes mellitus (GDM) is associated with increased risks of fetal overgrowth, possibly due to the increased … ith fatty acid transporter 1 (FATP1) mRNA expression (β = -1.38E-4, p = 0.036) in all participants. Lipidomics profiling demonstrated that maternal choline intake was negatively associated with concentrations of triglyceride species in the placenta regardless of GDM status. CONCLUSIONS: In conclusion, maternal choline intakes demonstrated negative associati …

Sex Differences in Urinary Metabolite Profiles between Survivors and Non-Survivors of Radiation-induced Lung Injury in the C57L/J Murine Model.

Novel biodosimetry assays are needed to categorize both acute ionizing radiation injury and delayed effects of radiation exposure, such as r … ols. Tissue injury was characterized using whole-body plethysmography, histopathology, and targeted lipidomics. Urinary metabolites were detected using untargeted metabolomic profiling to determine if they could serve as early predictors of RILI survival. A survival rate of 40-45% was observed at 180 days postirradiation consistent with the established LD50/ …

Normalizing Body Weight with a Dietary Strategy Mitigates Obesity-Accelerated Pancreatic Carcinogenesis in Mice.

BACKGROUND: Obesity is a modifiable risk factor for pancreatic cancer, but the impact of dietary changes leading to weight loss in pancreati … wk, then switched to CD for 13 additional wk (DIO→CD). Cancer incidence was evaluated by histology. Lipidomics and RNAseq followed by bioinformatic analysis identified potential mechanisms. The gut microbiome was characterized using 16s rRNA amplicon sequencing. Data were analyzed using 1-way analysis of variance. RESULTS: After 21 wk, DIO-fed mice had 1.7- …

Multi-omics profiles of chronic low back pain and fibromyalgia-Study protocol.

BACKGROUND: Chronic low back pain (CLBP) and fibromyalgia (FM) are leading causes of suffering, disability, and social costs. Current pharma … psychosocial function as moderator. Blood and urine samples are collected to analyze metabolomics, lipidomics and proteomics. We will integrate the overall omics data to identify common mechanisms and pathways, and associate multi-omics profiles to pain-related clinical characteristics, physical function, indicators of neuropathic pain, and pain facilitatio …

In vitro Assay to Examine the Function of Tears on Corneal Epithelial Cells.

Tears contain numerous secreted factors, enzymes, and proteins that help in maintaining the homeostatic condition of the eye and also protec … kers. Significant advancements have been made in recent years in the field of tear film proteomics, lipidomics, and transcriptomics to allow a better understanding of how tears can be utilized to gain insight into the etiology of diseases. These advancements have enabled us to study the pathophysiology of various disease states using tear samples. However, t …

Leptin-mediated suppression of lipoprotein lipase cleavage enhances lipid uptake and facilitates lymph node metastasis in gastric cancer.

BACKGROUND: Lymph node metastasis (LNM) is the primary mode of metastasis in gastric cancer (GC). However, the precise mechanisms underlying … lipid levels were quantified using oil red O staining, BODIPY 493/503 staining, and flow cytometry. Lipidomics analysis was employed to identify alterations in intracellular lipid composition following LPL knockdown. Protein expression levels were assessed through immunohistochemistry, Western blotting, and enzyme-linked immunosorbent assays. The mouse popli …