Результаты поиска

Spinal cord neural stem cells derived from human embryonic stem cells promote synapse regeneration and remyelination in spinal cord injury model rats.

Spinal cord injury (SCI) is a devastating injury that significantly impairs patients' quality of life. To date, there is no effective treatm … ed the expression of synaptophysin, a synaptic signature protein and MBP, a protein associated with remyelination. Interestingly, BAF45D, a chromatin remodelling factor that contributes to the induction of hSCNSCs with region-specific spinal cord identity, were increased by the hSCNSC transplantation. In addition, conditioned medium derived from the hSCNSCs als …

Baicalin facilitates remyelination and suppresses neuroinflammation in rats with chronic cerebral hypoperfusion by activating Wnt/β-catenin and inhibiting NF-κB signaling.

One main factor contributing to the cognitive loss in vascular dementia (VD) is white matter lesions (WMLs) carried on by chronic cerebral hypoperfusion (CCH). A secondary neuroinflammatory response to CCH accelerates the loss and limits the regeneration of oligodendrocytes, leading to progressive demyelination and insufficient remyelination in the white matter. Thus, promoting remyelination and inhibiting neuroinflammation may be an ideal therapeutic strategy. Baicalin (BAI) is known to exhibit protective effects against various inflammatory and demyelinating diseases. However, whether BAI ha…

Reduced axonopathy and enhanced remyelination after chronic demyelination in fibroblast growth factor 2 (Fgf2)-null mice: differential detection with diffusion tensor imaging.

Chronic central nervous system demyelinating diseases result in long-term disability because of limited remyelination capacity and cumulative damage to axons. Corpus callosum demyelination in mice fed cuprizone provides a reproducible model of chronic demyelination in which the demyelinating agent can be removed to test modifications that promote recovery and to develop noninvasive neuroimaging techniques for monitoring changes in myelin and axons. We used the cuprizone model in mice with genetic deletion of fibroblast growth factor 2 (Fgf2) to determine the impact of FGF2 on axon pathology an…

Automated quantification of axonal and myelin changes in contusion, dislocation, and distraction spinal cord injuries: Insights into targeted remyelination and axonal regeneration.

Quantifying axons and myelin is essential for understanding spinal cord injury (SCI) mechanisms and developing targeted therapies. This stud … ath-thickness-to-axon-diameter ratio, suggesting disrupted myelination. These results indicate that remyelination therapies may be most effective for distraction injuries, where preserved axons make remyelination crucial, while axonal regeneration therapies are likely better suited for dislocation injuries with extensive axonal loss. Contusion injuries, involvi …

Valproic acid effects on human adipose-derived stem cell differentiation into oligodendrocytes and improved remyelination in a mouse model of Multiple Sclerosis.

Valproic acid (VPA), a neuroprotective agent and inhibitor of GSK3-β, along with human Adipose-Derived Stem Cells (hADSCs) have been proposed to be potential therapeutic agents for neurodegenerative disorders. In the present study, we have assessed the effects of VPA alone or in combination with hADSCs on oligodendrocyte differentiation, remyelination, and functional recovery in a mouse model of Multiple Sclerosis (MS). These MS-model mice were randomly divided into cuprizone, sham, VPA, hADSC, and VPA/hADSC groups, with 10 mice considered a control group (healthy mice). The hanging wire test …

A Subpopulation of Foxj1-Expressing, Nonmyelinating Schwann Cells of the Peripheral Nervous System Contribute to Schwann Cell Remyelination in the Central Nervous System.

New myelin sheaths can be restored to demyelinated axons in a spontaneous regenerative process called remyelination. In general, new myelin sheaths are made by oligodendrocytes newly generated from a widespread population of adult CNS progenitors called oligodendrocyte progenitor cells (OPCs). New myelin in CNS remyelination in both experimental models and clinical diseases can also be generated by Schwann cells (SCs), the myelin-forming cells of the PNS. Fate-mapping studies have shown that SCs contributing to remyelination in the CNS are often derived from OPCs and appear not to be derived f…

Воздействие фингозина-1-фосфатных модуляторов рецепторов на ремиелионацию при множественном склерозе: данные экспериментов in vitro, модели животных и исследования человека.

Сфингосин-1-фосфат (S1P) является классом терапевтических агентов, используемых для лечения множественного склероза (MS). В настоящее время Управление по контролю за продуктами питания и лекарствами/Европейское медицинское агентство (FDA/EMA) утвердило четыре модулятора S1P: финголимод, сипонимоз, понизимод и озанимод. Помимо их хорошо зарекомендовавших себя иммунодуляционных свойств, накапливаемые данные свидетельствуют о том, что эти молекулы оказывают прямое воздействие на клетки потомков олигодендроцитов (ОРК) и зрелые олигодендроциты, содействуя ремонту миелина с помощью различных механиз

Совпадение в аннотации оригинала
Sphingosine-1-phosphate (S1P) receptor modulators are a class of therapeutic agents used for the treatment of multiple sclerosis (MS). There … show enhanced OPC differentiation and process extension, while in vivo models demonstrate increased remyelination, improved myelin protein expression, and functional recovery. Imaging studies in people with MS suggest increased myelin repair over time. Potential mechanisms underlying the observed effects involve selective modulation of S1P receptor subtypes (pa …

Buyang Huanwu decoction promotes gray and white matter remyelination by inhibiting Notch signaling activation in the astrocyte and microglia after ischemic stroke.

ETHNOPHARMACOLOGICAL RELEVANCE: Ischemic stroke causes damages to both gray and white matter, resulting in long-term motor impairments. Myelin repair is a promising strategy for poststroke motor rehabilitation. Buyang Huanwu Decoction (BHD) is a classical traditional Chinese medicine formula for managing the sequelae of ischemic stroke. Whether BHD benefits gray and white matter remyelination following stroke remains to be elucidated. AIM OF THE STUDY: The present study aimed to investigate the effects of BHD on the gray and white matter remyelination following ischemic stroke and further exp…

Quantitative magnetic resonance imaging reflects different levels of histologically determined myelin densities in multiple sclerosis, including remyelination in inactive multiple sclerosis lesions.

Magnetic resonance imaging (MRI) of focal or diffuse myelin damage or remyelination may provide important insights into disease progression and potential treatment efficacy in multiple sclerosis (MS). We performed post-mortem MRI and histopathological myelin measurements in seven progressive MS cases to evaluate the ability of three myelin-sensitive MRI scans to distinguish different stages of MS pathology, particularly chronic demyelinated and remyelinated lesions. At 3 Tesla, we acquired two different myelin water imaging (MWI) scans and magnetisation transfer ratio (MTR) data. Histopatholog…

Decellularized optic nerve functional scaffold transplant facilitates directional axon regeneration and remyelination in the injured white matter of the rat spinal cord.

Axon regeneration and remyelination of the damaged region is the most common repair strategy for spinal cord injury. However, achieving good outcome remains difficult. Our previous study showed that porcine decellularized optic nerve better mimics the extracellular matrix of the embryonic porcine optic nerve and promotes the directional growth of dorsal root ganglion neurites. However, it has not been reported whether this material promotes axonal regeneration in vivo. In the present study, a porcine decellularized optic nerve was seeded with neurotrophin-3-overexpressing Schwann cells. This f…

A porcine acellular nerve matrix membrane with dual neurotrophic and immunomodulatory functions accelerates peripheral nerve regeneration by coordinating Schwann cell activation and remyelination.

Repairing peripheral nerve injuries remains challenging because it requires biomaterials that can simultaneously modulate the adverse immune … egeneration characterized by orderly axonal regrowth, coordinated Schwann cell activity, and robust remyelination, achieving a near-complete myelinated nerve fiber regeneration rate. By synergistically integrating structural, neurotrophic, and immunomodulatory functions into a single biomimetic platform, SNCM actively orchestrates a pro-regenerative microenviro …

Recombinant rat heat shock protein 70 (rrHSP70) may promote remyelination by regulating microglia/macrophages and oligodendrocytes in a toxin-induced demyelination animal model.

Heat shock protein 70 (HSP70) can assemble with other extracellular matrix proteins to form deoxycholate-insoluble aggregates in multiple sclerosis (MS) lesions, impairing oligodendrocyte (OLG) maturation and remyelination. However, soluble HSP70 supports myelination. HSP70 may also promote bone marrow-derived macrophages (BMDMs) toward classically and alternatively activated phenotypes, which may regulate OLG maturation. Given the roles of HSP70 in OLG maturation and in shaping microglial/BMDM phenotypes in MS lesions, we investigated how HSP70 in microglia/macrophages regulates OLG maturatio…

Local low dose curcumin treatment improves functional recovery and remyelination in a rat model of sciatic nerve crush through inhibition of oxidative stress.

Traumatic injuries to peripheral nerves are frequent, however, specific pharmacological treatments are currently lacking. Curcumin has antioxidant, anti-inflammatory and neuroprotective properties but high oral doses are required for therapeutic use, particularly due to its low bioavailability. The aim of the present study was to investigate the effects of local and continuous treatment using low curcumin doses on functional recovery and nerve regeneration after rat sciatic nerve crush (SNC). Curcumin was administered by osmotic pumps with a catheter delivering the drug at the injury site (0.2

Оптический анализ функционального воздействия демиелинации и ремиэлизации Cuprizone на межсферную нервную коммуникацию в антериорной кортекс через Corpus Callosum.

Cuprizone (CPZ) — широко используемый токсин, который вызывает демиелинизирующие заболевания в моделях животных, производя множественный склероз (MS), похожий на патологию в грызунах. CPZ является одним из немногих токсинов, которые вызывают демиелинацию и последующее воспоминание после прекращения ее применения. В этом исследовании рассматриваются функциональные последствия демиэлизации, вызванной КПЗ, и последующее восстановление нейронной связи в передней коре сингула (АКК) с уделением особого внимания соединению между полушариями через корпус каллосум (CC). Используя широкополосную высокос

Совпадение в аннотации оригинала
Cuprizone (CPZ) is a widely used toxin that induces demyelinating diseases in animal models, producing multiple sclerosis (MS)-like pathology in rodents. CPZ is one of the few toxins that triggers demyelination and subsequent remyelination following the cessation of its application. This study examines the functional consequences of CPZ-induced demyelination…

Impact of Intraventricular Human Adipose-Derived Stem Cells Transplantation with Pregnenolone Treatment on Remyelination of Corpus Callosum in A Rat Model of Multiple Sclerosis.

OBJECTIVE: Multiple sclerosis (MS) is known as a nerve tissue disorder, which causes demyelination of central nervous system (CNS) fibers. Cell-based treatment is a novel strategy for the treatment of demyelinating diseases such as MS. Adipose-derived stem cells (ADSCs) have neuroprotective and neuroregenerative effects and pregnenolone as a neurosteroid has remarkable roles in neurogenesis. We intend to examine the impact of intraventricular transplantation of human ADSCs and systemic injection of pregnenolone on the remyelination of a rat model cuprizone-induced demyelination. MATERIALS AND…

Zuo Gui Wan Promotes Remyelination in Multiple Sclerosis by Attenuating MAPK-Mediated Microglial M1 Polarization, an Integrated Network Pharmacology and Experimental Validation Study.

PURPOSE: Zuo Gui Wan (ZGW) shows clinical potential in treating multiple sclerosis (MS), but its underlying mechanism of action remains elus … havioral performance was assessed using the rotarod, elevated plus-maze, and tail suspension tests. Remyelination was evaluated via LFB staining, TEM, and the expression of oligodendrocyte markers (Olig2, CNPase, MOG). Microglial activation/polarization (Iba-1, iNOS, CD86, Arg-1, CD206) and MAPK pathway proteins (p-ERK1/2, p-p38, and p-JNK) were quantified by W …

Demyelination/remyelination and expression of interleukin-1β, substance P, nerve growth factor, and glial-derived neurotrophic factor during trigeminal neuropathic pain in rats.

The etiology of trigeminal neuropathic pain is not clear, but there is evidence that demyelination, expression of cytokines, neuropeptides, … ell proliferation, and increased immunoreactivity of IL-1β and SP. Our data show that demyelination/remyelination processes are related to the development of pain behavior. IL-1β may have effects both in ganglia and nerves, while SP may be an important mediator at the nerve endings. Additionally, low levels of GDNF may produce impaired signaling, which may be i …

Monoclonal remyelination-promoting natural autoantibody SCH 94.03: pharmacokinetics and in vivo targets within demyelinated spinal cord in a mouse model of multiple sclerosis.

Chronic inflammatory demyelination of the central nervous system is usually incompletely repaired. However, we previously reported that in v … lesions as detected by light and electron microscopic autoradiography. These findings suggest that remyelination-promoting autoantibodies could act within the demyelinating lesion of the central nervous system by binding to the oligodendrocyte, myelin, or axon.

Taohong Siwu Decoction switches the detrimental/reparative phenotypes of astrocytes via RhoA/ROCK2/Profilin-1 axis to bolster oligodendrogenesis and remyelination after ischemic stroke.

ETHNOPHARMACOLOGICAL RELEVANCE: Promoting remyelination is crucial for recovery after ischemic stroke and astrocyte polarization toward detrimental A1 or reparative A2 phenotypes shapes the post-stroke microenvironment. Taohong Siwu Decoction (THSWD) has neuroprotective effects, but its impact on remyelination and astrocyte polarization remains unclear. RhoA/ROCK2/Profilin-1 (PFN1) signals may be key regulators. AIM OF THE STUDY: This research investigated whether THSWD promoted post-stroke remyelination by modulating astrocytic A1/A2 polarization via RhoA/ROCK2/PFN1 axis. MATERIALS AND METH…

Panax quinquefolius saponins promote remyelination via orchestrating HMGCS1-NPC1-MAL-mediated lipid metabolism and rebalancing JAK-STAT signaling in a cuprizone-induced demyelination model.

ETHNOPHARMACOLOGICAL RELEVANCE: Panax quinquefolius L. is traditionally used as a "Qi-tonifying and Yin-nourishing" herb for weakness and limb flaccidity, symptoms described as "Feng fei" or flaccidity syndrome. These manifestations partially resemble motor dysfunction in multiple sclerosis (MS). Panax quinquefolius Saponins (PQS), are major bioactive constituents, but their effects on demyelination and related molecular changes remains unclear. AIM OF THE STUDY: To investigate the effects of PQS on demyelination and explore associated changes in inflammatory signaling and lipid metabolism.