Результаты поиска

Potential of Lycii Radicis Cortex as an Ameliorative Agent for Skeletal Muscle Atrophy.

Lycii Radicis Cortex (LRC) is a traditional medicine in East Asia with various beneficial effects, including antioxidant, anti-inflammatory, anti-tumor, anti-diabetic, and anti-depressant properties. However, its potential effects on skeletal muscle atrophy have not been studied. In this study, the protective effects of LRC extract (LRCE) on dexamethasone (DEX)-induced muscle atrophy were investigated in C2C12 myotubes and mice. We evaluated the effect of LRCE on improving muscle atrophy using a variety of methods, including immunofluorescence staining, quantitative polymerase chain reaction (

Совпадение в полном тексте источника
… The amount of 150 kg of dried LRC was extracted two times with 2100 L of 50% ethanol at a temperature of 85 ± 5 °C for 6 h, additionally containing 1.6% citric acid. Afterward, the extract was evaporated in vacuo at 65 °C. …

Targeting homologous recombination and telomerase in Barrett's adenocarcinoma: impact on telomere maintenance, genomic instability and tumor growth.

Homologous recombination (HR), a mechanism to accurately repair DNA in normal cells, is deregulated in cancer. Elevated/deregulated HR is implicated in genomic instability and telomere maintenance, which are critical lifelines of cancer cells. We have previously shown that HR activity is elevated and significantly contributes to genomic instability in Barrett's esophageal adenocarcinoma (BAC). The purpose of this study was to evaluate therapeutic potential of HR inhibition, alone and in combination with telomerase inhibition, in BAC. We demonstrate that telomerase inhibition in BAC cells incre

Совпадение в полном тексте источника
… The cells were then washed again as described above and stained overnight in solution containing 1 mg/ml X-gal, 40 mM citric acid/sodium phosphate (pH 6), 5 mM potassium ferrocyanide, 150 mM NaCl, and 2 mM MgCl2. …

Окисляющий стресс и воспаление в патогенезе неврологических расстройств: механизмы и последствия.

Нейрозащита — это активный подход к защите нервной системы, включая мозг, спинной мозг и периферийные нервы, путем предотвращения или ограничения повреждения нервных клеток и других компонентов. Она защищает центральную нервную систему от повреждений от острых и прогрессивных нейродегенеративных расстройств. Окисляющий стресс, дисбаланс между естественными механизмами защиты тела и образованием реактивных видов кислорода, имеет решающее значение для развития неврологических расстройств. Из-за высокой скорости метаболизма и потребления кислорода мозг особенно уязвим к окисляющему стрессу. Чрезм

Совпадение в полном тексте источника
… Biochemical analysis of postmortem AD brains revealed impaired citric acid cycle enzyme function, correlated with clinical status, and linked to reduced brain metabolism 121 . In NDDs, mitochondrial dysfunction is a significant concern. …

Taste and smell disturbances in patients with chronic oral graft vs. host disease: An observational study.

BackgroundA common complication of allogeneic hematopoietic stem cell transplantation (alloHSCT) is chronic oral graft vs. host disease (cGvHD). Oral cGvHD may present as mucosal lesions, salivary gland dysfunction, and trismus. Moreover, taste and smell ability may be affected, but the prevalence, nature and severity of altered taste and smell function, and their impact on quality of life (QoL) are understudied.AimTo identify the prevalence, nature, and severity of taste and smell disturbances, their impact on QoL and to assess whether altered taste/smell ability is associated with oral mucos

Совпадение в полном тексте источника
… The 16 taste strips are impregnated with four different flavors in different concentrations: sweet (0.05, 0.1, 0.2, or 0.4 g/ml sucrose), salty (0.016, 0.04, 0.1, or 0.25 g/ml sodium chloride), sour (0.05, 0.09, 0.165, or 0.3 g/ml citric acid) or bitter (0.0004, 0.0009, 0.0024, and 0.006 g/ml quinine …

Metabolic Reprogramming of Cancer Cells and Therapeutics Targeting Cancer Metabolism.

BackgroundCancer metabolism is a field focused on the unique alterations in metabolic pathways that occur in cancer cells, distinguishing them from the metabolic processes in normal cells.MethodsAn extensive review of the current literature on the metabolic adaptation of cancer cells was carried out in the current study.ResultsThe rapidly proliferating cells require high levels of molecules, such as glucose, amino acids, lipids, and nucleotides, along with increased energy demand (ATP). These requirements are met through alterations in the processes involving glucose, amino acid, lipid, and nu

Совпадение в полном тексте источника
… Since ACLY is typically overexpressed in a range of cancers, one cancer treatment option is to alter the enzyme's citric acid binding site through indirect transcyclic citric acid‐binding. …

Metabolic reprogramming in cancer and senescence.

The rising trend in global cancer incidence has caused widespread concern, one of the main reasons being the aging of the global population. Statistical data show that cancer incidence and mortality rates show a clear upward trend with age. Although there is a commonality between dysregulated nutrient sensing, which is one of the main features of aging, and metabolic reprogramming of tumor cells, the specific regulatory relationship is not clear. This manuscript intends to comprehensively analyze the relationship between senescence and tumor metabolic reprogramming; as well as reveal the impac

Совпадение в полном тексте источника
… In the metabolic study of senescent cells, researchers found that senescent cells were able to further utilize oxidative phosphorylation metabolites such as citric acid, α‐ketoglutarate fumarate, and malic acid to enhance cellular mitochondrial respiration and generate large amounts of ROS, which ultimately … The primary metabolic processes taking place within mitochondria include the citric acid cycle, the breakdown of fatty acids through β‐oxidation, and the generation of ATP via oxidative phosphorylation. …

Alterations in macrophage polarization in the craniofacial and extracranial skeleton after zoledronate application and surgical interventions - an in vivo experiment.

PURPOSE: Medication-related osteonecrosis occurs exclusively in the jaw bones. However, the exact pathogenesis of medication-related osteonecrosis of the jaw (MRONJ) and the unique predisposition of the jaw bones have not been elucidated, making its treatment a challenge. Recent evidence indicates that macrophages might play a pivotal role in MRONJ pathogenesis. The aim of the present study was to compare the macrophage populations between the craniofacial and extracranial skeleton and to investigate the changes induced by zoledronate (Zol) application and surgical interventions. MATERIALS AN

Совпадение в полном тексте источника
… In the next step, slides were boiled in citric acid buffer (pH 6.0, Thermo Fisher Scientific, Waltham, Massachusetts, US) at 100°C for 30 min to retrieve antigens und cooled down for 30 min at room temperature. Then, slides were immersed in washing buffer. …

Generation and characterization of mAb 61H9 against junctional adhesion molecule-a with potent antitumor activity.

Junctional adhesion molecule-A (JAM-A) is an adhesion molecule that exists on the surface of certain types of cells, including white blood cells, endothelial cells, and dendritic cells. In this study, the cDNA sequences of JAM-A-Fc were chemically synthesized with optimization for mammalian expression. Afterward, we analyzed JAM-A protein expression through transient transfection in HEK293 cell lines. Mice were immunized with JAM-A-Fc protein, and hybridoma was prepared by fusing myeloma cells and mouse spleen cells. Antibodies were purified from the hybridoma supernatant and four monoclonal s

Совпадение в полном тексте источника
… Protein A resin, equilibration, and washing with PBS buffer (pH 7.5), then elution with 20 mM citric acid buffer (pH 2.7). Analysis was carried out SDS-PAGE of fractions of interest, qualitative and quantitative. …

A novel dual HDAC and HSP90 inhibitor, MPT0G449, downregulates oncogenic pathways in human acute leukemia in vitro and in vivo.

Acute leukemia is a highly heterogeneous disease; therefore, combination therapy is commonly used for patient treatment. Drug-drug interaction is a major concern of combined therapy; hence, dual/multi-target inhibitors have become a dominant approach for cancer drug development. HDACs and HSP90 are involved in the activation of various oncogenic signaling pathways, including PI3K/AKT/mTOR, JAK/STAT, and RAF/MEK/ERK, which are also highly enriched in acute leukemia gene expression profiles. Therefore, we suggest that dual HDAC and HSP90 inhibitors could represent a novel therapeutic approach fo

Узкие полифенолы в питании свиноматок: обновление.

Окисляющий стресс является потенциально критическим фактором, влияющим на производительность гектарирующих и кормящих свиней. Полифенолы представляют собой большой класс вторичных метаболитов растений, обладающих мощными антиоксидантами. Все полифенолы структурно характеризуются ароматическими кольцами с множеством гидроксилов водорода; они делают полифенолы идеальными атомами водорода и электронными донорами для нейтрализации свободных радикалов и других реактивных видов кислорода. В течение последнего десятилетия все большее внимание уделялось полифенолам в качестве функциональных кормовых д

Совпадение в полном тексте источника
… There were 632 genes differentially expressed in the liver of lactating sows compared to non-lactating sows; these altered genes are mainly related to amino acid metabolism, fatty acid metabolism, the citric acid cycle, and peroxisome proliferator-activated receptor (PPAR) signaling ( Rosenbaum et al …

Metabolic features of myeloma cells in the context of bone microenvironment: Implication for the pathophysiology and clinic of myeloma bone disease.

Multiple myeloma (MM) is a hematological malignancy characterized by the accumulation of malignant plasma cells (PCs) into the bone marrow (BM). The complex interaction between the BM microenvironment and MM PCs can lead to severe impairment of bone remodeling. Indeed, the BM microenvironment exerts a critical role in the survival of malignant PCs. Growing evidence indicates that MM cells have several metabolic features including enhanced glycolysis and an increase in lactate production through the upregulation of glucose transporters and enzymes. More recently, it has been reported that MM ce

Совпадение в полном тексте источника
… Citric acid and bone metabolism. Biochem J (1952) 52(2):260–5. doi: 10.1042/bj0520260 70. Esen E, Chen J, Karner CM, Okunade AL, Patterson BW, Long F. Wnt-Lrp5 signaling induces warburg effect through Mtorc2 activation during osteoblast differentiation. …

Monoclonal Antibodies-Anchored Quantum Dots-Based Delivery Strategies for Glioblastoma Treatment: Challenges and Applications.

Treatment of glioblastoma multiforme (GBM) has been a great challenge before medical fraternity since last century owing to a median survival of less than 15 months, despite of intensive therapy. Neurosurgeries, intense chemotherapy, advanced radiotherapy, and targeted therapies have bought some extension to the life of GBM patients. Combination and targeted therapies could bring a concrete approach to tackle the complexities of GBM treatment. Monoclonal antibodies (mAbs) have already proved their potential, owing to their high affinity and target-specificity, as a promising cancer immunothera

Совпадение в полном тексте источника
… The lateral size of citric acid monohydrate (C.A.) QDs (CA-GQDs) was 6.36 nm before PEGylation, and it increased by approximately 24.10 nm after PEGylation. Cane sugar (C.S.) GQDs had a larger particle size (136.75 nm), which increased above 200 nm after PEGylation. …

Iron in the Tumor Microenvironment-Connecting the Dots.

Iron metabolism and tumor biology are intimately linked. Iron facilitates the production of oxygen radicals, which may either result in iron-induced cell death, ferroptosis, or contribute to mutagenicity and malignant transformation. Once transformed, malignant cells require high amounts of iron for proliferation. In addition, iron has multiple regulatory effects on the immune system, thus affecting tumor surveillance by immune cells. For these reasons, inconsiderate iron supplementation in cancer patients has the potential of worsening disease course and outcome. On the other hand, chronic im

Совпадение в полном тексте источника
… In addition, cellular iron availability impacts on mitochondrial respiration, ATP (for adenosine triphosphate) and mitochondrial radical formation, but also controls cellular metabolism and aerobic glycolysis via its regulatory effects on citric acid cycle enzymes ( 7 , 8 ). … Iron-dependent changes in cellular energy metabolism: influence on citric acid cycle and oxidative phosphorylation . Biochim Biophys Acta ( 1999 ) 1413 : 99 – 107 . 10556622 10.1016/s0005-2728(99)00088-2 8. Volani C Doerrier C Demetz E Haschka D Paglia G Lavdas AA . …

Piperine: From Green Extraction to Clinical Translation-A Review.

BACKGROUND: Piperine has diverse pharmacological and bioenhancing activities, but poor aqueous solubility, variable exposure, interaction liability, and discontinuity across production, formulation, and clinical evaluation limit translation. This review integrates these frequently separated stages. METHODS: A structured narrative PubMed search covered English-language studies published from 1 January 2021 to 27 April 2026, supplemented by ClinicalTrials.gov records checked through 13 June 2026, backward citation searching, and selected earlier seminal studies. Evidence was synthesized by expe

Dasatinib as a bone-modifying agent: anabolic and anti-resorptive effects.

BACKGROUND: Bone loss, in malignant or non-malignant diseases, is caused by increased osteoclast resorption and/or reduced osteoblast bone formation, and is commonly associated with skeletal complications. Thus, there is a need to identify new agents capable of influencing bone remodeling. We aimed to further pre-clinically evaluate the effects of dasatinib (BMS-354825), a multitargeted tyrosine kinase inhibitor, on osteoblast and osteoclast differentiation and function. METHODS: For studies on osteoblasts, primary human bone marrow mensenchymal stem cells (hMSCs) together with the hMSC-TERT

Совпадение в полном тексте источника
… In vivo model For in vivo studies, dasatinib powder was dissolved in sterile 80 mM citric acid pH 2.1 to make a 10 mg/mL stock solution and then further dilutions were made in 80 mM sodium citrate pH 3.1. Thirty 5-week-old female CD1 healthy mice were housed at our Animal Care Facility. …

Glutamine and cancer: metabolism, immune microenvironment, and therapeutic targets.

Glutamine is the most abundant amino acid in human serum, and it can provide carbon and nitrogen for biosynthesis, which is crucial for proliferating cells. Moreover, it is widely known that glutamine metabolism is reprogrammed in cancer cells. Many cancer cells undergo metabolic reprogramming targeting glutamine, increasing its uptake to meet their rapid proliferation demands. An increasing amount of study is being done on the particular glutamine metabolic pathways in cancer cells.Further investigation into the function of glutamine in immune cells is warranted given the critical role these

Совпадение в полном тексте источника
… Entry of glucose- and glutamine-derived carbons into the citric acid cycle supports early steps of HIV-1 infection in CD4 T cells. Nat Metab. 2019;1(7):717–30. 10.1038/s42255-019-0084-1. 57. Ma G, et al. …

Navigating Stomatologic Complications Secondary to Antineoplastic Agents-A Comprehensive Review.

This review systematically examines the oral complications associated with conventional and novel anti-cancer therapies. It highlights that while molecularly targeted agents including monoclonal antibodies targeting the vascular endothelial growth factor and its receptor, the epidermal growth factor receptor, tyrosine kinase inhibitors, and immune checkpoint inhibitors tend to exhibit a lower overall toxicity profile compared to traditional cytotoxic chemotherapeutics, they are nonetheless linked to significant oral adverse events. These complications encompass inflammatory mucosal reactions k

Совпадение в полном тексте источника
… Ascorbic, malic, and citric acid-rich foods can enhance saliva flow for radiation xerostomia. However, their acidity might harm oral tissues, worsen mucositis, and aid tooth demineralization [ 73 , 74 ]. 3.2.2. …

Clinical and Therapeutic Implications of Male Obesity.

The prevalence of obesity, a disorder linked to numerous comorbidities and metabolic complications, has recently increased dramatically worldwide and is highly prevalent in men, even at a young age. Compared to female patients, men with obesity more frequently have delayed diagnosis, higher severity of obesity, increased mortality rate, and only a minority of obese male patients are successfully treated, including with bariatric surgery. The aim of this review was to present the current state of knowledge about the clinical and therapeutic implications of obesity diagnosed in males.

Совпадение в полном тексте источника
… It is worth mentioning that cellulose and citric acid in an oral, non-systemic, superabsorbent hydrogel are used in conjunction with diet and exercise to aid weight management in obese or overweight adults. …

Properties of Approved Antitumour Chinese Herbal Medicines: Integrating Evidence and Tradition.

The properties and meridian affinities of Chinese herbal medicine are closely associated with their therapeutic efficacy. This review focuses on approved antitumour Chinese medicines listed in the Pharmacopoeia of the People's Republic of China (2020 Edition, Volume I), highlighting their key components. Statistical methods were used to analyse the properties, flavours and channel tropisms of antitumour traditional Chinese medicine (TCM), including both proprietary formulations and commercially available individual herbs. Distinctive patterns were identified by comparing herb profiles with con

Биоразлагаемый электроспун PLGA Nanofibers-Encapsolated Trichinella Sphiralis Antigens защищает от повторного использования экспериментального аутоиммунного энцефаломилита и связанного с ним микробиота дисбиоз.

ЦЕЛЬ: " Тричинелла спиралис " создала сложные механизмы иммуномодуляции при посредничестве экскременто-секретарских продуктов (ESL1), которые позволяют ей выжить в принимающей стране. Следовательно, антигены ESL1 обладают прекрасным потенциалом для лечения аутоиммунных заболеваний, таких, как множественный склероз (MS). Однако вопрос о том, могут ли своевременные контролируемые поставки антигенов ESL1 в vivo, как и в случае естественных инфекций, повысить его терапевтический потенциал в отношении MS, до сих пор неизвестен.Для этого мы ввели антигены ESL1 в полихлорированные (лактидно

Совпадение в полном тексте источника
… Additionally, the slides were prepared for epi-fluorescent microscopy by antigen retrieval in citrate buffer (10mM Citric Acid, 0.05% Tween 20, pH 6.0) at 95°C for 20 min, followed by spontaneous cooling to room temperature. …