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Cancer-Related Alopecia Risk and Treatment.

Opinion statementCancer-related alopecia (CRA) presents a significant challenge for many patients undergoing cancer treatment, often affecting their psychological well-being and sense of identity. In my opinion, the optimal management of CRA requires a proactive, personalized approach that prioritizes both prevention and regrowth, while taking into account the type of cancer therapy, patient goals, and overall clinical context. For patients receiving chemotherapy, especially taxane- or anthracycline-based regimens, scalp cooling should be offered as a first-line preventative option whenever fe

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… Various compounds, particularly biotin and specific hair growth supplements, have been explored for regrowth, although research has largely focused on patients with androgenetic alopecia and alopecia areata rather than patients experiencing cancer-related alopecia [ 76 , 77 ]. … Nutritional supplements such as biotin and nutraceuticals are widely used, though evidence supporting their efficacy remains limited. …

Витамины в профилактике и терапии нейродегенеративных заболеваний.

Нейродегенеративные заболевания, такие, как болезнь Альцгеймера (AD), болезнь Паркинсона (PD), амиотропический боковой склероз (ALS) и множественный склероз (MS), которые являются следствием постепенной потери нейронной функции и структуры, вызывают значительное когнитивное расстройство. Заболеваемость этими заболеваниями среди населения мира постоянно растет в результате старения населения. Хотя генетические и экологические факторы чаще всего упоминаются в качестве патогенетических факторов этих заболеваний, все больше фактов свидетельствует о важной роли надлежащего питания в профилактике и

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… Vitamin B7, also known as biotin, is crucial for metabolism as a coenzyme for carboxylases that aid in synthesizing fatty acids, amino acids, and glucose. … The positive effects of high-dose vitamin B7 in progressive MS are thought to stem from the increased activity of biotin-dependent enzymes involved in myelin fatty acid synthesis and mitochondrial energy metabolism [ 31 ]. …

The effects of 1 alpha,25-dihydroxyvitamin D(3) on matrix metalloproteinase and prostaglandin E(2) production by cells of the rheumatoid lesion.

INTRODUCTION: 1alpha,25-dihydroxyvitamin D(3)[1alpha,25(OH)(2)D(3)], the biologically active metabolite of vitamin D3, acts through an intra … Samples were embedded in paraffin, and examined for presence of VDRs by immunolocalisation using a biotinylated antibody and alkaline-phosphatase-conjugated avidin-biotin complex system. Cultured synovial fibroblasts and chrondrocytes were treated with either 1alpha,25(OH)(2)D(3) or interleukin (IL)-1beta, or both. Conditioned medium was assayed for MMP …

Advances and Prospects of Prolamine Corn Protein Zein as Promising Multifunctional Drug Delivery System for Cancer Treatment.

Zein is a type of prolamine protein that is derived from corn, and it has been recognized by the US FDA as one of the safest biological materials available. Zein possesses valuable characteristics that have made it a popular choice for the preparation of drug carriers, which can be administered through various routes to improve the therapeutic effect of antitumor drugs. Additionally, zein contains free hydroxyl and amino groups that offer numerous modification sites, enabling it to be hybridized with other materials to create functionalized drug delivery systems. However, despite its potential

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… coupling of biotin and ATRA to the zein shell had the highest internalization and cytotoxicity in A549 lung cancer cells compared to single biotin-targeted nanocarriers and non-targeted nanocarriers. … vitro: The coupling of biotin and ATRA to the zein shell had the highest internalization and cytotoxicity in A549 lung cancer cells compared to single biotin-targeted nanocarriers and non-targeted nanocarriers. …

Applications of Aggregation-Induced Emission Materials in Immunology: From Diagnostics to Immunotherapy.

Aggregation-induced emission (AIE) materials, due to their unique ability to significantly enhance optical emission in aggregated states, have demonstrated vast potential in immunology, particularly in early disease diagnosis and immunotherapy. AIE materials can serve as highly efficient fluorescent probes for biomarker detection, providing critical insights for early diagnosis. Additionally, they can act as cancer vaccines, enhancing the effectiveness of immunotherapy through photodynamic therapy, photothermal therapy, or activation of immune cells. This review highlights the applications of

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… Liu et al. synthesized a biotin-conjugated H4TCPE as an AIE-active molecule and assembled it onto the surface of mesoporous silica nanoparticles, resulting in biotin-enriched H4TCPE dendritic mesoporous silica nanoparticles (BDMSNs) ( Figure ). … Xiong et al. introduced a novel two-step fluorescence immunoassay that employs antibody/streptavidin dual-labeled polystyrene nanobeads in conjunction with biotin-labeled G-quadruplex/hemin DNAzymes exhibiting peroxidase-like activity. …

Increased Circulating Soluble Junctional Adhesion Molecules in Systemic Sclerosis: Association with Peripheral Microvascular Impairment.

Systemic sclerosis (SSc, scleroderma) is a severe disease characterized by peripheral microcirculation abnormalities manifesting with Raynaud's phenomenon, nailfold videocapillaroscopic (NVC) changes, and even ischemic digital ulcers (DUs) that are often refractory to treatments. In the wake of previously described associations between the circulating levels of soluble junctional adhesion molecules (sJAMs) and SSc clinical features, here, we measured sJAM-A and sJAM-C levels by enzyme-linked immunosorbent assay in serum samples from a large case series of 110 SSc patients and 85 healthy contro

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… Assay for Serum sJAM-A Serum levels of sJAM-A were quantified by commercial quantitative colorimetric sandwich ELISA using a 96-well microtiter plate precoated with a capture antibody specific to sJAM-A and a biotin-conjugated detection antibody (catalog no. … The liquid was then removed without washing, and the wells were incubated for 1 h at 37 °C with 100 µL of detection reagent A (biotin-conjugated detection antibody). …

Radiometals in Imaging and Therapy: Highlighting Two Decades of Research.

The present article highlights the important progress made in the last two decades in the fields of molecular imaging and radionuclide therapy. Advancements in radiometal-based positron emission tomography, single photon emission computerized tomography, and radionuclide therapy are illustrated in terms of their production routes and ease of radiolabeling. Applications in clinical diagnostic and radionuclide therapy are considered, including human studies under clinical trials; their current stages of clinical translations and findings are summarized. Because the metalloid astatine is used for

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… In 1999, Paganelli et al. published the first clinical preliminary results of [ 90 Y]Y-DOTA-biotin for the treatment of high-grade gliomas ( n = 48) based on biotin–streptavidin chemistry and showed tumor reduction (>25–100%) in 25% of patients; in 16% of these, the response lasted for at least a year … In 2000, a phase II clinical trial of [ 90 Y]Y-DOTA-biotin was reported in patients with metastatic colon cancer [ 239 ]. …

Клемастин фумарат ускоряет аккумуляцию инвалидности в прогрессивном множественном склерозе, усиливая пироптоз.

Множественный склероз (MS) — иммунопосредованное демиелинизирующее заболевание CNS. Клемастин фумарат, внезапный антигистамин и мускариновый блокатор рецепторов, обладает ремиелионирующим потенциалом в MS. Климастичная рука была добавлена в текущее клиническое исследование платформы, нацеленное на остаточную активность на основе точности, биомаркированной комбинированной терапии множественных склерозов (TRAP-MS) (Clinical Procures.gov). NCT03109288), для выявления сигнатуры ремиелинирования цереброспиновой жидкости (CSF) и для сбора данных о безопасности клемастина у пациентов независимо от ре

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… The cells were stabilized for 24 hours in comp:OM1 medium (DMEM/F12 containing B27 supplement [1×], N2 supplement [1×], GlutaMax [1×], nonessential amino acids [1×], 2-mercaptoethanol, 25 μg/mL insulin, 60 ng/mL T3, 100 ng/mL biotin, 1 μM cAMP, 20 ng/mL PDGF-AA, 5 ng/mL FGF-basic, 100 nM retinoic acid … Next the cells were differentiated for 8 days in complete maturation medium (DMEM/F12 containing B27 supplement [1×], N2 supplement [1×], GlutaMax [1×], nonessential amino acids [1×], 2-mercaptoethanol, 25 μg/mL insulin, 60 ng/mL T3, 100 ng/mL biotin, 1 μM cAMP, 5 ng/mL NT-3, 10 ng/mL IGF-3 and 1 μg/ …

Chemical Proteomics and Phenotypic Profiling Identifies the Aryl Hydrocarbon Receptor as a Molecular Target of the Utrophin Modulator Ezutromid.

Duchenne muscular dystrophy (DMD) is a fatal muscle-wasting disease arising from mutations in the dystrophin gene. Upregulation of utrophin to compensate for the missing dystrophin offers a potential therapy independent of patient genotype. The first-in-class utrophin modulator ezutromid/SMT C1100 was developed from a phenotypic screen through to a Phase 2 clinical trial. Promising efficacy and evidence of target engagement was observed in DMD patients after 24 weeks of treatment, however trial endpoints were not met after 48 weeks. The objective of this study was to understand the mechanism o

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… A click reaction is performed to ligate a fluorophore or enrichment tag, such as biotin, to the probe. … Figure 4 Target validation using immunoblotting and fluorescence. a) Immunoblot of AfBPP samples following treatment of human DMD myoblast cells and biotin enrichment. …

Advanced Diagnostic Techniques

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… Numerous tissues have endogenous avidin-biotin activity that must be blocked before adding biotinylated reagents in avidin-biotin detection systems. … Current IHC methods can be divided into avidin-biotin or non–avidin-biotin systems. After incubation with the primary antibody, a secondary antibody specific for the primary antibody (secondary reagent) is added. For avidin-biotin systems, the secondary reagent is biotinylated. …

TSP-1 interaction with RANK and OPG: implications for bone remodeling and osteolytic bone metastasis.

Excessive bone disruption, driven by upregulation of bone-degrading osteoclasts, occurs in several pathologies, including breast cancer osteolytic bone metastasis, a condition associated with poor prognosis and diminished quality of life for patients. The matricellular protein thrombospondin-1 (TSP-1) plays pleiotropic roles in physiological and pathological remodeling of several tissues, including bone, and in shaping the microenvironment of primary tumors and metastasis. This study aimed to explore the role of TSP-1 in bone remodeling associated with osteolytic bone metastasis. We have ident

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… After washing, plates were incubated with DELFIA Enhancement Solution, and bound biotin-labeled ligands were quantified measuring time-resolved fluorescence using a Victor3 multilabel plate reader (PerkinElmer). … In other experiments, biotin-labeled OPG (0.1 μg) was incubated with recombinant HTRA1 (R&D System, 0.1 µg) for 24 h at 37 °C. Degradation of OPG was evaluated by western blot analysis with ExtrAvidin-Peroxidase. …

The Emergence of Universal Immune Receptor T Cell Therapy for Cancer.

Chimeric antigen receptor (CAR) T cells have shown great success in the treatment of CD19+ hematological malignancies, leading to their recent approval by the FDA as a new cancer treatment modality. However, their broad use is limited since a CAR targets a single tumor associated antigen (TAA), which is not effective against tumors with heterogeneous TAA expression or emerging antigen loss variants. Further, stably engineered CAR T cells can continually and uncontrollably proliferate and activate in response to antigen, potentially causing fatal on-target off-tumor toxicity, cytokine release s

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… This can either be done through the use of natural binding partners, such as avidin-biotin or leucine zippers, or through binding of an anti-tag CAR to its cognate antigen tag. … Further work on the biotin-specific platform led to the use of a high affinity monomeric streptavidin extra cellular domain ( 53 ). …

CAR T-cell therapy in mature lymphoid malignancies: clinical opportunities and challenges.

The advent of chimeric antigen receptor T-cell (CAR T-cell) therapy has revolutionized the treatment paradigm of various hematologic malignancies. Ever since its first approval for treatment of acute lymphoblastic leukemia (ALL) in 2017, CAR T-cell therapy has been found to be efficacious in various other lymphoid malignancies, with recent approvals in diffuse large B-cell lymphoma (DLBCL) and mantle cell lymphoma (MCL). Although CAR T-cell therapeutics offer a novel immunotherapeutic approach to treat otherwise refractory malignancies, the plethora of studies/products and complexities in manu

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… The BBIR CAR system uses a biotinylated antigen-specific molecule, which tags tumor antigen containing tumor cells for recognition by the biotin-binding T-cell with an extracellular avidin domain ( Figure 4A ). … Antigen specific biotinylated molecules, which can couple with biotin binding immune receptor. (Adapted from Urbanska K, et al. Cancer Res. 2012); (B) Structure of SUPRA CAR. …

Antibody-based drug delivery systems for cancer therapy: Mechanisms, challenges, and prospects.

In recent years, antibody-based cancer therapy has emerged as one of the efficient therapeutic strategies, such as immune checkpoint inhibitors (ICIs), angiogenesis inhibitors, antibody-drug conjugates (ADCs), multi-specific antibodies, and chimeric antigen receptor T (CAR-T) cells, among others. To date, various drug delivery platforms have been developed to improve the bioavailability, delivery convenience, and reduced toxicity towards increased therapeutic efficacy of antibodies. Herein, we emphasize the clinical manifestations of various antibody-based tumor therapies, highlighting their m

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… General methods include coupling antibodies to materials using biotin-binding proteins, Fc-binding proteins, and aptamers. Therein, avidin and streptavidin proteins offer strong binding forces to biotin, which are not easily affected by external conditions, such as pH and temperature 67 . … In addition, Fc-binding peptides and protein G/A are simpler ways to immobilize antibodies than biotin-binding proteins. Notably, the mild reaction conditions and simple purification process can maintain the activity and stability of the antibody 68 . …

Перспективы Талкетамаба и его полезности в лечении множественной миеломы: безопасность, эффективность и место в терапии.

Несмотря на недавние достижения, большинство пациентов с множественной миэломой в конечном итоге развивают сопротивление имеющимся методам лечения, подчеркивая необходимость новых терапевтических стратегий. G Класс рецептора с протеиновыми соединениями C 5 Член D (GPRC5D) стал жизнеспособной новой терапевтической целью для клеток миэломы, которая привела к клиническому развитию талькетамаба, первого GPRC5D-directed bi специфичный Т-активист (TCE). В августе Талкетамаб получил ускоренное утверждение 2023 Управление по контролю за продуктами питания и лекарствами. Помимо ожидаемой краткосрочной

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… Other strategies such as mineral and vitamin supplementation with zinc and biotin, salivary stimulants, and oral hydration have been used. Dose modifications, including reductions, delays, or skips, were helpful management strategies for dysgeusia. … Emollients are commonly used, as well as vitamin E oil, systemic hydration, biotin, and protective nail coverings (eg socks and gloves). Avoidance of nail hardeners, acrylics, and gels is recommended as these may increase the risk of onychomycosis. …

Острый инфекционный мононуклеоз порождает стойкие, функциональные антитела EBNA-1 с высокой поперечной реактивностью до альфа-кристаллической бета.

Мы исследуем масштаб, специфичность и функциональные свойства ядерного антигена 1 (EBNA-1) вируса Эпштейн-Барр (EBV) у молодых людей в ходе первичной инфекции. Обнаружены специфические связующие антитела EBNA-1, а также антитела, способные к осаждению клеточного фагоцитоза (ADCP) и дополняющего антитела (ADCD). Эти антитела в первую очередь направлены против региона EBNA-1, который, как известно, вызывает перекрестные антитела для нескольких самопептидов. У лиц, имеющих по крайней мере один аллеле HLA-DRB1*15:01, отмечаются высшие связующие и ADCD антитела EBNA-1. Альфакристалллин бета (КРИАБ)

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… Due to limitations in multiplexing, antibody-dependent cellular phagocytosis (ADCP) assays were performed on only three peptides using a neutravidin-biotin interaction system. … IgG3 PE [HP6050] Southern Biotech Cat #9210–09, RRID:AB_2796701 Biological samples LowTox Guinea Pig Complement CedarLane Labs Cat # CL4051 Chemicals, peptides, and recombinant proteins Human soluble FcγR2A Duke University Custom Order Human soluble FcyR3A Duke University Custom Order LC-LC-Sulfo-NHS Biotin …

Emerging Target Discovery Strategies Drive the Decoding of Therapeutic Power of Natural Products and Further Drug Development: A Case Study of Celastrol.

Celastrol (CEL) is a natural pentacyclic triterpenoid demonstrating significant therapeutic properties against various diseases. However, the ambiguity of target information poses a significant challenge in transitioning CEL from a traditional remedy to a modern pharmaceutical agent. Recently, the emerging target discovery approaches of natural products have broadened extensive avenues for uncovering comprehensive target information of CEL and promoting its drug development. Herein, diverse target discovery strategies are overviewed for the pharmacological and toxicological studies of CEL, inc

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… Presently, the commonly used CEL probes are biotinylated probes and bioorthogonal probes, primarily synthesized through a covalent reaction involving the carboxyl group at the C‐20 position with a biotin or bioorthogonal group (Figure 2B,C ). … Additionally, the inclusion of a free biotin control is essential to eliminate potential interference from biotin labeling on NPs, thereby ensuring the precision of target identification. …

Understanding Mesangial Pathobiology in AL-Amyloidosis and Monoclonal Ig Light Chain Deposition Disease.

Patients with plasma cell dyscrasias produce free abnormal monoclonal Ig light chains that circulate in the blood stream. Some of them, termed glomerulopathic light chains, interact with the mesangial cells and trigger, in a manner dependent of their structural and physicochemical properties, a sequence of pathological events that results in either light chain-derived (AL) amyloidosis (AL-Am) or light chain deposition disease (LCDD). The mesangial cells play a key role in the pathogenesis of both diseases. The interaction with the pathogenic light chain elicits specific cellular processes, whi

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… Avidin-biotin technique, diaminobenzidine as marker. Original magnification ×700. (h–j) In vivo rat model of LCDD. (h) Periodic acid–Schiff (PAS) stain showing expanded mesangial with increased PAS-positive extracellular matrix (circles). … Avidin-biotin technique, diaminobenzidine as marker. Original magnification ×700. (h–j) In vivo rat model of LCDD. (h) Periodic acid–Schiff (PAS) stain showing expanded mesangial with increased PAS-positive extracellular matrix (circles). …

Адипоцитный β-гидроксибутират обеспечивает метаболическую стойкость в множестве миеломы.

Множественная миелома (ММ) остается неизлечимым раком крови. Ожирение является известным фактором риска, но то, как адипоциты способствуют прогрессу MM, не в полной мере понятно. Здесь мы обнаруживаем метаболический перекресток между адипоцитами и клетками MM, который способствует выживанию клеток MM под глюкозой. Мы показываем, что ограничение глюкозы активирует АМПК, срывает связь HSP90-IRF4 и делает IRF4 восприимчивым к протеасомной деградации при помощи TRIM21. Парадоксально, но такой же стресс стимулирует адипоциты к производству β-гидроксибутирата (β-OHB). Клетки MM используют β-OHB при

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… To biochemically validate these findings, we designed biotin-conjugated peptides spanning IRF4 residues 74-101, including an acetylated K87 variant (Biotin-K87ac) and an unmodified control (Biotin-K87). … Pulldown assays using lysates from HEK293T cells expressing Flag-HSP90 and Strep-TRIM21 demonstrated that Biotin-K87ac precipitated significantly more HSP90 and less TRIM21 than Biotin-K87 (Supplementary Fig. 6h ), providing direct evidence that K87 acetylation promotes HSP90 binding and disrupts TRIM21 …

Dietary interventions for multiple sclerosis-related outcomes.

BackgroundMultiple sclerosis (MS) is a common demyelinating disease of the central nervous system. Although the exact pathogenesis remains u … (10 trials); dietary programmes (3 trials); and other dietary supplements (e.g. acetyl L-carnitine, biotin, creatine, palmitoylethanolamide, probiotic, riboflavin) (6 trials). In three trials comparing PUFAs with monounsaturated fatty acids (MUFAs), the evidence was very uncertain concerning difference in relapses (risk ratio (RR) 1.02, 95% confidence in …