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Histopathological Study of Breast Cancer at the Oran University Hospital Center on a Population from Western Algeria.

UNLABELLED: Breast cancer (BC) is the most common cancer among women worldwide and also in Algeria. Despite therapeutic progress, it remains a cancer with serious physical and psychological consequences. MATERIALS AND METHODS: The objective of this study was to perform a histological analysis on 244 patients from the western Algerian population who were receiving treatment at the Oran University Hospital. RESULTS AND DISCUSSION: The findings of our study indicates that women are more likely to be affected, with a predominance of tumors in the right breast (77%), we also found that (72%) of p…

Occurrence of Leishmania spp. in phlebotomine sand flies and dogs in Guelma region, North-eastern Algeria.

Leishmania spp. are sand fly-borne parasitic protozoa of worldwide distribution that may severely affect the health and welfare of dogs as well as of other mammalian species, including humans. Algeria is among the most affected countries, counting several cases of Leishmania infantum infection in humans and dogs. Here, we assessed the occurrence of Leishmania species in both phlebotomine sand fly and dogs in the Guelma region, Northeast of Algeria. Sand flies were collected from July to September 2022, followed by a survey of canine leishmaniasis (CanL) from September 2023 to February 2024. Ad…

Rabies in the Endemic Region of Algeria: Knowledge, Attitude and Practice (KAP) Survey among University Students.

(1) Background: Rabies is a neglected zoonosis considered to be one of the most significant health threats in the world, responsible of thousands of deaths globally. Algeria is one of the main countries of concern, with more than 15 deaths and more than 100,000 bites by rabid-suspected animals being reported annually. This study was conducted to evaluate the level of knowledge, attitudes and practice (KAP) toward this disease among university students in the endemic region of Algeria. (2) Methods: a cross-sectional online survey was conducted between June 2023 and March 2024 using a self admin…

Molecular detection and MST genotyping of Coxiella burnetii in ruminants and stray dogs and cats in Northern Algeria.

Aiming at identifying the reservoir and contamination sources of Coxiella burnetii in Northern Algeria, we investigated the molecular presence of the bacterium in 599 samples (blood, placenta, liver, spleen, and uterus) collected from cattle, sheep, dogs and cats. Our qPCR results showed that 15/344 (4.36%) blood samples and six/255 (2.35%) organ specimens were positive for C. burnetii. In cattle, three (4%) blood and liver samples were positive. In sheep, one blood (1.19%) and 3 (8.57%) placenta samples were positive. At the Algiers dog pound, 8 (10%) and 3 (5%) blood samples were qPCR positi…

Correlation of biological cervical cancer with its demographic and obstetric parameters in Ain Defla region, Algeria.

This study aims to determine the prevalence of cervical dysplasia and its relationship to biological and demographic characteristics. A transversal observational study is based on 236 conventional smears collected in Algeria. A Pap smear was taken, fixed and then stained using Papanicolaou staining. The interpretation of the results was done using the Bethesda 2014 system. Some of the patients with abnormal smears had colposcopy and, if necessary, a biopsy. Other patients with low-grade lesions were recommended to have their smears resumed 6 months or 1 year later. Cytological analysis was per…

Молекулярная идентификация и оценка генетического разнообразия коксиэллоподобных эндосимбионтов, переносимого клещами скота в Алжире.

Напоминающие коксиеллу бактерии представляют собой большую группу еще до изолата и характеризуют бактерии филогенетически близко к агенту Q лихорадки, Coxiella signetii, и часто ассоциируются с иксоидными клещами по всему миру. Цель этого исследования состояла в том, чтобы оценить присутствие эндосимбионтов, подобных коксиелле, в клещах и описать их генетическое разнообразие в различных видах клещей, поражающих скот в Алжире. Из трех мест было собрано в общей сложности 765 клещей. При отборе 20% отобранных клещей (147/765) было выявлено, что в 51,7% (76/147). Секвенирование частичных 16S rRNA

Совпадение в аннотации оригинала
Coxiella-like bacteria are a large group of yet-to-isolate and characterize bacteria phylogenetically close to the agent of Q fever, Coxiell … CLE) in ticks and to describe their genetic diversity in different tick species infesting cattle in Algeria. A total of 765 ticks were collected from three locations. The screening of 20 % of sampled ticks (147/765) exhibited the presence of Coxiella-like in 51.7 % (76/147). The sequencing of partial 16S rRNA and the GroEl genes showed an identity higher …

First report of Chlamydophila abortus infection in the dromedary camel (Camelus dromedarius) population in eastern Algeria.

Chlamydiosis is caused by an obligate intracellular gram-negative bacterium of the genus Chlamydophila which is a zoonotic pathogen. The objectives of the study were to identify the seroprevalence of antibodies against Chlamydophila abortus in dromedary camel herds from four districts in eastern Algeria, as well as to estimate the association between seroprevalence and certain factors present at the animal and herd levels. Blood samples were collected from a random sample of animals within each of 82 camel herds. Serum samples were subjected to a C. abortus ELISA test, and association between …

Isolation and identification of Legionella spp. from hot spring water in Algeria by culture and molecular methods.

AIMS: Due to infectious risk associated with the presence of Legionella in warm water, we determined the prevalence of living Legionella spp. in hot spring water in Algeria. METHODS AND RESULTS: Detection of Legionella by culture was done by using two methods, direct culture on agar plates and co-culture with amoeba. Fifty samples were taken from different hot springs in northern Algeria, including swimming pools, showers and thermal sources. Legionella pneumophila serotypes were predominant, accounting for 60% of positive samples. Direct method allowed the isolation of 13 L. pneumophila only…

Fasciolosis due to Fasciola hepatica in ruminants in abattoirs and its economic impact in two regions in Algeria.

and February 2009. The aim of the study was to determine the prevalence of infected livers with fascioliasis and to evaluate the economic loss due to the condemnation of livers infected with fascioliasis. A total of 3,457 cattle, 6,161 sheep and 5,764 goats were slaughtered and examined in El Tarf abattoir, north Algeria (humid climate), and 2,151 cattle, 5,724 sheep, 351 goats and 582 camels in Ouargla abattoir, south Algeria (saharan climate). In El Tarf region, the prevalence of fascioliasis infection was 26.7±2.5%, 6.5±0.4% and 2.5±0.2% in cattle, sheep and goats, respectively (P< 0.001). …

Association of CAT-262C/T with the concentration of catalase in seminal plasma and the risk for male infertility in Algeria.

Catalase (CAT) plays a central role in the protection of different cell types against the deleterious effects of hydrogen peroxide. In human, CAT is implicated in many physiological and pathological conditions including idiopathic male infertility. In this study we examined the association between CAT levels in seminal plasma with different sperm parameters and with CAT-262 C/T polymorphism and their risk for idiopathic male infertility in Algeria. Semen and blood samples were obtained from 111 infertile males and 104 fertile controls from the region of Eastern Algeria following informed conse…

Serogrouping and antibiotic resistance of Escherichia coli isolated from broiler chicken with colibacillosis in center of Algeria.

AIM: Colibacillosis is considered as one of the major bacterial infections in avian pathology. The excessive use of antibiotics reduced their effectiveness, which eventually led to the risk of emergence of antibiotic resistance. The aim of this study was to isolate, identify and serotype the pathogenic Escherichia coli strains and to determine their antibiotic susceptibility. MATERIALS AND METHODS: A total of 180 samples from different organs of broilers with colibacillosis lesions were collected (liver, spleen, lung, and heart) in center of Algeria. The isolation and identification of E. col…

Detection of relapsing fever Borrelia spp., Bartonella spp. and Anaplasmataceae bacteria in argasid ticks in Algeria.

BACKGROUND: Argasid ticks (soft ticks) are blood-feeding arthropods that can parasitize rodents, birds, humans, livestock and companion animals. Ticks of the Ornithodoros genus are known to be vectors of relapsing fever borreliosis in humans. In Algeria, little is known about relapsing fever borreliosis and other bacterial pathogens transmitted by argasid ticks. METHODOLOGY/PRINCIPAL FINDINGS: Between May 2013 and October 2015, we investigated the presence of soft ticks in 20 rodent burrows, 10 yellow-legged gull (Larus michahellis) nests and animal shelters in six locations in two different …

Prevalence of Fasciola hepatica in Galba truncatula detected by Multiplex PCR in the province of El Tarf (Algeria).

The prevalence of Fasciola hepatica infection in the snail Galba truncatula was studied for the first time in Algeria using Multiplex PCR. A total of 722 individuals were collected from 11 typical habitats (temporary ponds, road ditches and puddles) distributed in five localities of the El Tarf province, known for endemicity of fascioliasis. Snails were divided in 75 groups and DNA extraction was performed using Chelex® (Biorad, Nazareth Eke). Two primers were used, the first is specific for 124 bp DNA fragment of Fasciola sp and the second one represents the ITS-2 lymnaeids sequence (500-600 …

Molecular characterization of Echinococcus granulosus sensu stricto and Echinococcus canadensis in humans and livestock from Algeria.

In Algeria, previous studies investigated genotypes of Echinococcus granulosus sensu lato in animals and identified E. granulosus sensu stricto (s.s.) genotypes G1 and G3 whereas Echinococcus canadensis genotype G6 was only reported from dromedary cysts. Molecular data on human cystic echinococcosis (CE) were limited. We implemented a large genotyping study of hydatid cysts from humans and livestock animals to specify CE's molecular epidemiology and the genetic diversity in Algeria. Fifty-four human CE cysts from patients predominantly admitted in surgical units from Mustapha Hospital, Algiers…

Prevalence and characterization of extended spectrum β-lactamases in Klebsiella-Enterobacter-Serratia group bacteria, in Algeria.

OBJECTIVES: The authors had for aim to assess the local epidemiology, antibiotic resistance, and molecular typing of expanded spectrum betalactamase producing Klebsiella, Enterobacter, and Serratia (ESBL KES). MATERIALS AND METHODS: Two hundred and seven strains of the KES group were isolated in the microbiology laboratory of the Annaba Ibn Rochd hospital in 2009. The antibiotic resistance (diffusion method and MIC) was tested and ESBL detection was performed as recommended by the Clinical Laboratory Standard Institute (CLSI). The characterization of genes for resistance to β-lactams (CTX-M-1

Genetic characterization of Fasciola hepatica from Tunisia and Algeria based on mitochondrial and nuclear DNA sequences.

Fasciolosis caused by Fasciola spp. (Platyhelminthes: Trematoda: Digenea) is considered the most important helminth infection of ruminants in tropical countries, causing considerable socioeconomic problems. In the present study, samples identified morphologically as Fasciola hepatica from sheep and cattle from different geographical locations of Tunisia and Algeria were genetically characterised by sequences of the first (ITS-1), the 5.8S and second (ITS-2) Internal Transcribed Spacers (ITS) of nuclear ribosomal DNA (rDNA) and mitochondrial Cytochrome c Oxidase subunit I (COI) gene. Comparison…

Real-time PCR assay for the identification of cutaneous Leishmania parasite species in Constantine region of Algeria.

We evaluated the use of real-time PCR for the identification of cutaneous Leishmania species. The assay, based on the melting curve analysis of fluorescent products, allows the discrimination of four culture adapted strains (L. infantum MHOM/TN/80/IPT1, L. major MHOM/SU/73/5-ASKH, L. donovani MHOM/IN/80/DD8 and L. tropica MHOM/SU/74/K27). One hundred and twenty-nine skin lesions, spotted on filter paper, were collected from patients consulting for suspicion of cutaneous leishmaniasis (CL) at the parasitology laboratory of Constantine Hospital (Algeria). Ninety-seven (75.2%) of the samples anal…

Genotypic and phenotypic characterization of potential virulence of intestinal avian Escherichia coli strains isolated in Algeria.

In order to characterize potential pathogenic Escherichia coli strains isolated from diarrheic hens and chickens originating from intensive battery rearing in North Algeria, the presence of a large range of virulence factors and markers was studied in 50 strains by DNA-DNA hybridization on colonies and phenotypic tests. The sequences we focused on were those coding for adhesins F5, F41, F17, Pap, Afa, and Sfa; intimin Eae; and toxins STa, STb, LT1, Stx1, Stx2, CNF1, and CNF2. The phenotypes explored were the colicins, aerobactin, hemolysins, and hemagglutinin production and serum resistance. T…

At least five polymorphic mutants account for the prevalence of glucose-6-phosphate dehydrogenase deficiency in Algeria.

The electrophoretic mobility and level of enzyme activity of glucose-6-phosphate dehydrogenase (G6PD) was established in 100 unrelated Algerian males with G6PD deficiency. DNA from these subjects was analysed for the presence of certain known G6PD mutations by the appropriate restriction enzyme digestion of fragments amplified by the polymerase chain reaction. Where the mutation could not be identified in this way, the samples were subjected to single-strand conformation polymorphism analysis and abnormal fragments were sequenced. In this way, eight different mutations have been identified, of…

[A new enzymatic variant of Leishmania infantum Nicolle, 1908, agent of cutaneous leishmaniasis in northern Algeria].

For the first time, the pathogenic agent of cutaneous leishmaniasis in the North of Algeria has been identified as Leishmania infantum s.1. The parasite was found to be a newly discovered enzymatic variant (zymodeme 24) differing by two electromorphs from a variant isolated in France (zymodeme 11) from the same type of lesion. Until now, the dermotropic zymodemes of Algeria and France have not been seen in cases of visceral leishmaniasis of the Mediterranean Basin.