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Curcumin and Its Derivatives as Anticancer Agents in Head and Neck Cancer: Molecular Mechanisms and Preclinical Evidence

Head and neck cancer (HNC), particularly oral squamous cell carcinoma (OSCC), remains a major clinical challenge due to its aggressive behavior, high recurrence rates, and frequent resistance to conventional therapies. Natural compounds, especially curcumin and its derivatives, have gained increasing attention as potential anticancer agents due to their ability to target multiple molecular pathways involved in tumor progression. This review critically evaluates the current preclinical and translational evidence supporting curcumin and its derivatives as monotherapeutic agents in HNC, with part

Targeting the HPGDS-PGD2 axis to treat Th17 cell-mediated autoimmunity in multiple sclerosis.

Th17 cells are central drivers of autoimmune pathology, including multiple sclerosis (MS), yet the endogenous metabolic signals that shape their pathogenic differentiation remain incompletely defined. Prostaglandin D₂ (PGD2) is traditionally viewed as an extracellular mediator in allergic diseases. Here, we identify PGD2 as a cell-intrinsic antagonist of the nuclear receptor PPARγ, thereby promoting Th17 cell differentiation and exacerbating EAE pathology. PGD2 is selectively elevated in patients with MS compared with healthy individuals. Within the MS cohort, PGD2 correlates with Th17 cell fr

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… Mouse Naïve CD4 + T cells were sorted by a biotin selection kit (Miltenyibiotec, 130-104-454) from spleen or LN of C57BL/6 mice. … Human T cells were isolated from peripheral blood cells by Ficoll gradient centrifugation (Ficoll-Paque PLUS, GE Healthcare) and then sorted by a biotin selection kit (Miltenyibiotec, 130-096-533). …

The various therapeutic applications of the medical isotope holmium-166: a narrative review.

Over the years, a broad spectrum of applications of the radionuclide holmium-166 as a medical isotope has been established. The isotope holmium-166 is attractive as it emits high-energy beta radiation which can be used for a therapeutic effect and gamma radiation which can be used for nuclear imaging purposes. Furthermore, holmium-165 can be visualized by MRI because of its paramagnetic properties and by CT because of its high density. Since holmium-165 has a natural abundance of 100%, the only by-product is metastable holmium-166 and no costly chemical purification steps are necessary for pro

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… 2012 ; Nogueira and Campos 2016 ; Hosseini et al. 2013 ; Valente and Campos 2010 ; Won et al. 2005 ) Membranes | (Nogueira and de Campos 2012 ) Generator | DTPA complex | (Smith et al. 1995 ) Macroaggregates | (Makela et al. 2003a ; Park et al. 1996 ; McLaren et al. 1990 ; Sledge et al. 1977 ) DTPA-Biotin … Labeling of biotin with [166Dy]Dy/166Ho as a stable in vivo generator system. Int J Pharm. 2003;255(1–2):129–138. doi: 10.1016/S0378-5173(03)00052-8. Foote H, Jr, Landon H, Sailor V. Slow neutron resonances in holmium, thulium, and lutetium. Phys Rev. 1953;92(3):656. doi: 10.1103/PhysRev.92.656. …

Biomarkers in Multiple Sclerosis: An Up-to-Date Overview

During the last decades, the effort of establishing satisfactory biomarkers for multiple sclerosis has been proven to be very difficult, due to the clinical and pathophysiological complexities of the disease. Recent knowledge acquired in the domains of genomics-immunogenetics and neuroimmunology, as well as the evolution in neuroimaging, has provided a whole new list of biomarkers. This variety, though, leads inevitably to confusion in the effort of decision making concerning strategic and individualized therapeutics. In this paper, our primary goal is to provide the reader with a list of the

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… Moreover, a small series of patients with MS found decreased CSF and serum levels of the vitamin biotin, in comparison to healthy controls [ 88 ]. 6.3. Biomarkers of Cellular Subpopulations 6.3.1. … Cerebrospinal fluid levels of biotin in various neurological disorders. Acta Neurologica Scandinavica. 1999;99(6):387–392. doi: 10.1111/j.1600-0404.1999.tb07369.x. 89. Kuenz B, Lutterotti A, Ehling R, et al. Cerebrospinal fluid B cells correlate with early brain inflammation in multiple sclerosis. …

Диметил фумарат отменяет гепатоцеллюлярный карциномный рост, подавляя ось Nrf2/Bcl-xL и повышая эффективность сорафениба.

Гепатоклеточная карцинома (ГК) характеризуется плохим прогнозом и остается одной из основных причин смертности от рака во всем мире. Усовершенствованный КЦУК регулируется несколькими методами лечения первой линии, включая ингибиторы тиросина киназы (TKI) и иммунную терапию (mAb-PD-1 и mAb-VEGF). Вместе с тем эффективность лечения КЦХВ зачастую недолговечна. Недавние исследования показали, что активация траектории Nrf2-Bcl-xL способствует плохому прогнозу у группы пациентов с КЦХВ. Здесь мы обнаружили, что диметилфумарат (DMF), лекарство, используемое для лечения псориаза и множественного склер

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… Terminal deoxytransferase-mediated dUTP-biotin nick-end labeling (TUNEL-Alexa 640) assay was used to detect cell apoptosis in GFP-expressing cell lines. Cells were fixed with 4% paraformaldehyde for 20 min. …

The Potential Role of Proinflammatory Cytokines and Complement Components in the Development of Drug-Induced Neuropathy in Patients with Multiple Myeloma.

The launch of novel chemotherapeutic agents-in particular, proteasome inhibitors and immunomodulatory drugs-dramatically changed multiple myeloma (MM) therapy, improving the response rate and prolonging progression-free survival. However, none of the anti-MM drugs are deprived of side effects. Peripheral neuropathy (PN) seems to be one of the most pressing problems. Despite extensive research in this area, the pathogenesis of drug-induced peripheral neuropathy (DiPN) has not yet been fully elucidated. In the present study, we aimed to assess the potential relationship between proinflammatory f

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… Then, the detection-biotin-antibody cocktail (50 µL/well) was added to the plate and incubated for 1 h under the same conditions as in the first step. In the last step, Streptavidin–PE was added and incubated for 30 min under the same conditions as in the first step. …

The Adiponectin-AdipoR1 Axis Mediates Tumor Progression and Tyrosine Kinase Inhibitor Resistance in Metastatic Renal Cell Carcinoma.

The survival of patients diagnosed with metastatic renal cell carcinoma (RCC) is still limited and the current targeted therapies are only partially effective. Herein, we investigated the clinical value and functions of adiponectin receptors (AdipoR1 and AdipoR2) in metastatic renal cell carcinoma (RCC) patients treated with tyrosine kinase inhibitors (TKIs). A total of 127 mRCC patients treated with first-line TKIs between 2008 and 2017 at a single institution were collected. AdipoR1 and AdipoR2 expression was assessed by immunohistochemistry. AdipoR1 was positively expressed in 87.4% (111/12

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… The sections were subsequently incubated with corresponding secondary antibodies at room temperature for 1 hour, followed by peroxidase-streptavidin-biotin complex and diaminobenzidine to visualize the proteins. …

Bone Marrow Stromal Antigen 2 Is a Novel Plasma Biomarker and Prognosticator for Colorectal Carcinoma: A Secretome-Based Verification Study.

BackgroundThe cancer cell secretome has been recognized as a valuable reservoir for identifying novel serum/plasma biomarkers for different cancers, including colorectal cancer (CRC). This study aimed to verify four CRC cell-secreted proteins (tumor-associated calcium signal transducer 2/trophoblast cell surface antigen 2 (TACSTD2/TROP2), tetraspanin-6 (TSPAN6), bone marrow stromal antigen 2 (BST2), and tumor necrosis factor receptor superfamily member 16 (NGFR)) as potential plasma CRC biomarkers.MethodsThe study population comprises 152 CRC patients and 152 controls. Target protein levels in

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… After removing the liquid, 100 μ L of biotin-antibody was added to each well and incubated at 37°C for 1 hour. After adequate aspiration and washing, 100 μ L of HRP-avidin was added to each well and incubated at 37°C for 1 hour. Repeat the aspiration/wash process, and add 90 μ L of TMB substrate. …

Overexpression of GPER1 suppressed esophageal carcinoma growth via activating cAMP pathway.

G protein-coupled estrogen receptor 1 (GPER1) has extensively verified as a tumor regulator in various types of cancers. However, its role in esophageal cancer (EC) remains largely unclear. In this study, the expression and prognostic prediction value of GPER1 in EC was analyzed by using TCGA database and was verified in EC cells and fresh tissues. The results showed that GPER1 is decreased in EC cells and tissues, and lower GPER1 expression is associated with poor overall survival of EC patients. CCK-8 assay and flow apoptosis cytometry were applied to measure the ability of proliferation and

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… Briefly, 50 μL cell supernatant and 50 μL biotin antigen working solution were mixed and incubate at 37°C for 30 min. Next, 50μL avidin -HRP was added and incubated at 37°C for 30 min. …

Когнитивное расстройство, настроение и усталость в различных подтипах склероза: a one- последующее исследование.

Множественные подтипы склероза (MS) - релапсирующие реактивные (RRMS), вторичные прогрессивные (SPMS) и первичные (PPMS) - были связаны с различными профилями когнитивных нарушений с прогрессивными подтипами, в отличие от RRMS, с указанием дополнительных дефицитов в более широко распространенных областях. Исследования были в основном сосредоточены на РУМС, в результате чего СМПС и СМПС были недооценены из-за их более низкой распространенности и ограниченной лечебной направленности. Данные о взаимозависимости между когнитивным расстройством, настроением и усталостью с течением времени также нед

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… since onset (years) 11.82 8.75 18.23 8.30 10.82 7.25 F (2, 63) = 5.39, p  =.007 Time since diagnosis (years) 8.18 6.22 14.64 8.09 6.14 4.49 F (2, 63) = 10.4, p  < 0.001 Current therapy No therapy 5 22.7% 9 40.9% 12 54.6% First line 13 59.1% 5 22.7% Second line 4 18.2% 3 13.6% Third line 2 9.1% 7 31.8% Biotin …

Recent Trends in anti-tumor mechanisms and molecular targets of celastrol.

Celastrol, a compound derived from traditional Chinese medicine, has therapeutic effects and has been used to treat inflammation-related diseases, cancer, cardiovascular diseases, and neurodegenerative diseases. However, current reviews lack a comprehensive and systematic summary of the anti-tumor mechanisms and molecular targets of celastrol. For this reason, this paper reviews the anticancer properties of celastrol and the molecular mechanisms underlying its anticancer effects. This paper primarily focuses on the mechanism of action of celastrol in terms of inhibition of cell proliferation a

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… Klaić and colleagues synthesized and developed an active biotinated celastrol probe and performed affinity pull-down experiments with PANC-1 cell lysates to identify cell targets of celastrol, in which Annexin II and eEF1A were identified. … Chen et al. combined biotin-labeled celastrol (bio-celastrol) with recombinant proteins on a HuProtTM human protein microarray to identify potential celastrol-binding proteins in gastric cancer cells. …

Selinexor, a selective inhibitor of nuclear export, shows anti-proliferative and anti-migratory effects on male germ cells in vitro.

BackgroundSelinexor (SLX), a selective inhibitor of nuclear export (SINE), has been shown to interfere with nuclear export mechanisms and to exert antitumor effects in a variety of cancer cell types. It is known to regulate multiple fundamental cellular processes, including the DNA damage response, cell proliferation, and stress signaling pathways. Nevertheless, its potential effects on reproductive cells remain inadequately characterized. The present study was aimed to investigate the cytotoxic, apoptotic, anti-proliferative and anti-migratory effects of SLX on GC1 (spermatogonia) and GC2 (sp

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… Following PBS washes, secondary antibodies conjugated with biotin-streptavidin-peroxidase (Cat#: TP-125-HL, Thermo, UK) were added and incubated for 30 min. …

Inhibition of MALAT1 facilitates ROS accumulation via the Keap1/HO-1 pathway to enhance photodynamic therapy in secondary hyperparathyroidism.

The prevalence of secondary hyperparathyroidism (SHPT) in advanced chronic kidney disease (CKD) exceeds 80 %. Our previous study indicated that photodynamic therapy (PDT) has potential for treating SHPT. Long noncoding RNA (lncRNA) is involved in various oxidative stress and apoptotic processes, but the molecular mechanism remains unreported. In this study, we found that PDT induced apoptosis in SHPT through reactive oxygen species (ROS) accumulation. The expression of metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) and heme oxygenase 1 (HO-1) within SHPT was upregulated after

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… Biotin-labeled sense and antisense MALAT1 probes were supplied by Genecreate Biological Engineering Co., LTD. The probes were mixed with streptavidin beads (Thermo Fisher, USA) in total cell lysate and shaken at 4 °C overnight. …

Porphyromonas gingivalis усугубляет экспериментальный аутоиммунный энцефаломиэлит, проводя дифференциацию TH1 с помощью сигнала ZAP70/NF-IB.

Множественный склероз (MS) характеризуется хроническим воспалением и обезвоживанием в центральной нервной системе (CNS), главным образом в результате ненормальной активации периферической иммунной системы, в частности клеток TH1. Как основной патоген в периодонтрите, Porphyromonas gingivalis (P. gingivalis) связан с повышенным риском множественных склерозных прогрессий; однако его роль в центральной нервной системе воспаления остается неясной. В этом исследовании мы стремились определить, поощряет ли P. gingivalis периферическую дифференциацию клеток TH1 по сигнальному маршруту ZAP70/NF-InB, т

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… After washing, the membrane was incubated with a biotinylated goat anti-rabbit secondary antibody [Biotin-SP AffiniPure Goat Anti-Rabbit IgG (H + L), Jackson ImmunoResearch, USA; 1:200,000] for 1 h at 37°C, followed by 0.1 µg/mL peroxidase-conjugated streptavidin (Jackson ImmunoResearch, USA). …

Progressive multiple sclerosis: Evaluating current therapies and exploring future treatment strategies.

Progressive forms of multiple sclerosis (MS) include primary progressive MS (PPMS) and secondary progressive MS (SPMS). Unlike relapsing-remitting MS (RRMS), progressive MS is recognized by relentless progression with accumulating disability, rare to no relapses nor new activity on MRIs. Clinically, neurologic worsening in MS can occur in the relapsing-remitting (RRMS) phase of disease due to incomplete recovery from neuroinflammatory relapses. However, a progressive disease course is the dominant factor related to accumulating disability. There is persistent central nervous system (CNS) compa

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… For example, biotin and opicinumab showed early potential but failed to demonstrate significant benefit in later-stage trials, and domperidone increased prolactin without impacting disease progression [ [107] , [108] , [109] ]. …

Thalidomide attenuates radiation-induced apoptosis and pro-inflammatory cytokine secretion in oral epithelial cells by promoting LZTS3 expression.

Radiation-induced oral mucositis (RIOM) is a prevalent oral complication that occurs in individuals undergoing radiotherapy or radiation treatment for head and neck tumors. The presence of oral mucosal rupture and ulcerative lesions, which are the defining features of this condition, can significantly affect the quality of life of patients. Additionally, it can interfere with tumor therapy and contribute to an unfavorable prognosis. Current evidence suggests that cellular inflammation and programmed cell death are important factors in disease development. Moreover, thalidomide (THD) has been r

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… The slides treated with streptavidin biotin HRP complex were further treated with DAB chromogenic agent and stained with hematoxylin. Statistical analysis Data are expressed using mean ± standard deviation from a minimum of three distinct experiments. …

Synthetic nickel-containing superoxide dismutase attenuates para-phenylenediamine-induced bladder dysfunction in rats.

Para (p)-phenylenediamine and its toxic metabolites induce excess reactive oxygen species formation that results in bladder voiding dysfunction. We determined the effects of synthetic Ni-containing superoxide dismutase mimics and the role of oxidative stress in p-phenylenediamine-induced urinary bladder dysfunction. P-phenylenediamine (60 μg/kg/day) was intraperitoneally administered for 4 weeks to induce bladder injury in female Wistar rats. Synthetic Ni-containing superoxide dismutase mimics, WCT003 (1.5 mg/kg) and WCT006 (1.5 mg/kg), were then intraperitoneally administered for 2 weeks. Tra

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… Secondary antibodies (Super Sensitive TM Non-Biotin polymer HRP IHC) were used for detection (BioGenex, San Ramon, CA, USA). The signal was visualized by incubation with liquid diaminobenzidine tetrahydrochloride. …

Отчет о ДИД 1st Ежегодный Всемирный конгресс биомедицинской инженерии, проводимый в Сиане, Китай, 9-11 ноябрь 2017.

Мы рады представить на этом заседании резюме доклада "ДИД" 1st Всемирный конгресс биомедицинской инженерии 2017которая была проведена в Сиане в Китае [...].

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… Utilizing this technology, we investigate two different approaches for functionalizing label-free optical waveguide based biosensors: (a) surface modification with amine-based functional polymers (biotin-modified polyethylenimine (PEI-B)) employing active ester chemistry and (b) modification with dextran … Both kinds of capture molecules were immobilized on the PEI-B by means of streptavidin-biotin affinity binding. …

8(th) Annual European Antibody Congress 2012: November 27-28, 2012, Geneva, Switzerland.

The 8th European Antibody Congress (EAC), organized by Terrapin Ltd., was again held in Geneva, Switzerland, following on the tradition established with the 4th EAC. The new agenda format for 2012 included three parallel tracks on: (1) naked antibodies; (2) antibody drug conjugates (ADCs); and (3) bispecific antibodies and alternative scaffolds. The meeting started and closed with three plenary lectures to give common background and to share the final panel discussion and conclusions. The two day event included case studies and networking for nearly 250 delegates who learned of the latest adva

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… Conjugation with maleimide-PEG2-biotin resulted in up to 50–60% conjugation efficiency. It was found that incomplete conjugation was due to formation of mixed disulfide bonds with existing cysteines in the inter-chain disulfide bonds. Dr. … Lloyd showed results with new THIOMAB variants, which lack inter-chain disulfide bonds, yielding over 90% conjugation with maleimide-PEG2-biotin. …

Связь между окисляющим стрессом, митохондриальной дисфункцией, железным метаболизмом и микроглией в множестве склерозов: обзор нарации.

В последние годы в патогенезе множественного склероза особое внимание уделяется митохондриальным процессам, влияющим на начало заболевания. Окисляющий стресс будет одним из последствий митохондриальной дисфункции, и его воздействие на ткани мозга хорошо описано. Микроглия, как макрофаж мозга, имеет важную функцию в удалении нежелательных метаболитов, а также железа, который является усилителем окислительного стресса. Существуют новшества с точки зрения связи между этими процессами, которые в большей степени перенаправляют исследования на сам процесс нейродегенерации, с тем чтобы больше не дела

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… Biotin deficiency has been observed in multiple sclerosis. The use of oxygen is still being considered in therapy [ 7 ]. It has been found that TNF-α affects oxidative phosphorylation via calcium levels. …