Результаты поиска

Phalangeal quantitative ultrasound technology and dual energy X-ray densitometry in patients with primary hyperparathyroidism: influence of sex and menopausal status.

Fifty-one patients with surgically proven primary hyperparathyroidism (PHPT), 11 males and 40 females, mean age+/-SD: 55.9+/-14.1 years, and 58 age- and sex-matched normal subjects were studied. The femoral and L(2)-L(4) bone mineral density (BMD; Hologic QDR 4500 C), as well as quantitative ultrasonometry (QUS; DBM-Sonic 1200) of the phalanges of both hands were measured in patients and controls. QUS measurements included amplitude-dependent speed of sound (AD-SoS), and other parameters derived from the graphic trace: signal dynamics (Sdy), first wave amplitude (FWA), bone transmission time (

Множество миеломных клеточных липидов повышают целевость и эффективность нанолипосомных формул In Vitro.

Множественная миелома (MM) — рак крови, характеризующийся накоплением аномальных плазменных клеток в костном мозге (BM). Неопластическое расширение постепенно нарушает нормальное производство клеток крови, вытесняя здоровые кровеносные клетки. Болезнь характеризуется неизмеримо дифференцированными клетками В, которые выделяют антитела как часть адаптивной иммунной реакции. Одной из серьезных угроз, с которой сталкивается терапия малых молекул наркотиков для MM, является отсутствие селективной наркодиагностики, что в конечном счете приводит к недостаточному накоплению лекарств для целевых клето

Совпадение в полном тексте источника
… Sonication significantly reduced general particle sizes for LE-modified and control nanoliposomes, producing more uniform populations with time. The measurements for all sonicated drug-loaded nanoliposomes revealed two different effects on molecular size. … Nanoliposomes were sonicated using an Ultrasonic Bath- Branson model 5800 purchased from Fisher Scientific (Hanover Park, IL, USA). Sonication times varied as a function of the components in preparations (typically between 30 and 60 min). …

Hedgehog signaling in cancer stem cells: a focus on hematological cancers.

The stem cell paradigm was first demonstrated in hematopoietic stem cells. Whilst classically it was cytokines and chemokines which were believed to control stem cell fate, more recently it has become apparent that the stem cell niche and highly conserved embryonic pathways play a key role in governing stem cell behavior. One of these pathways, the hedgehog signaling pathway, found in all organisms, is vitally important in embryogenesis, performing the function of patterning through early stages of development, and in adulthood, through the control of somatic stem cell numbers. In addition to

Towards Precision Oncology: The Role of Smoothened and Its Variants in Cancer.

The G protein-coupled receptor Smoothened (Smo) is a central signal transducer of the Hedgehog (Hh) pathway which has been linked to diverse forms of tumours. Stimulated by advancements in structural and functional characterisation, the Smo receptor has been recognised as an important therapeutic target in Hh-driven cancers, and several Smo inhibitors have now been approved for cancer therapy. This receptor is also known to be an oncoprotein itself and its gain-of-function variants have been associated with skin, brain, and liver cancers. According to the COSMIC database, oncogenic mutations o

Обобщение и оценка воздействия нанопчастей аторвастатина и аторвастатина на вызванную ими демиелинацию Cuprizone: модуляция прохода с сигналами Nrf2/NF-kB.

Множественный склероз (MS) является хроническим воспалительным заболеванием центральной нервной системы (CNS), характеризующимся повреждением миелинской оболочки и, со временем, постепенным неврологическим расстройством. Статины привлекли внимание к исследованиям в области MS из-за их антивоспалительных и нейрозащитных эффектов. ЦЕЛИ: В этом исследовании оценивалось, может ли аторвастатин улучшить поведение и гистопатологические изменения мозга мужчины C57BL/6 Мыши. Все группы мышей, за исключением контрольной группы, которая получила нормальное питание, были накормлены 0.2% (КПЗ) в их ежеднев

Совпадение в полном тексте источника
… Preparation of Nano-Atorvastatin Nano-atorvastatin was prepared using a modified thin-film hydration and sonication method. Briefly, 72 mg of lecithin and 24 mg of atorvastatin calcium were dissolved in 2.4 mL of chloroform to obtain a homogeneous lipid–drug mixture. … To achieve the desired nanoparticle size, the suspension underwent a multistep sonication process: 1 minute of bath sonication, followed by 4 to 5 minutes of probe sonication (Sonopuls Ultrasonic Homogenizer HD 2070, Berlin), and a final 20 minutes of bath sonication. …

AF1q inhibited T cell attachment to breast cancer cell by attenuating Intracellular Adhesion Molecule-1 expression.

China appears to consistently lag behind developed countries like the US, Japan, and the nations of Europe in the development of pharmaceuticals, putting China in an embarrassing situation. In fact, China is still dependent on foreign imports for most highly effective cures to major diseases such as cancer, diabetes, hepatitis, and neurodegenerative disease. There is no denying the fact that governmental support, and especially a signifi cant amount of fi nancial support and political assistance to include government restructuring, is needed for the establishment of new drug Research and Devel

Проверьте оптические датчики на основе молекулярного распознавания SSWNT для воспламенительного цитокина TNF-α.

TNF-α (Tumor Necrosis Factor) — цитокиновый цитокин, который усиливает подстрекательскую реакцию и поощряет вербовку лейкоцитов. TNF-α производится главным образом с помощью активированных макрофагов, в частности, в ответ на инфекцию, воспаление или повреждение тканей. С учетом его центральной роли в нормальном и ненормальном иммунном реагировании он является объектом целого ряда терапевтических средств, таких, как адалимумаб и этицерцеп. TNF-α также является прогнозирующим и диагностическим биомаркером, связанным с Rheumatoid Artritis, болезнью Альцгеймера, множественным склерозом, нескольким

Совпадение в полном тексте источника
… nanotubes (SWCNT) (NanoIntegris Technologies, Boisbriand, Quebec) were suspended in solution separately with seven oligonucleotide sequences, described below ( Table 1 ; Integrated DNA Technologies, Coralville, IA), at a 1:2 SWCNT:ssDNA mass ratio in 1X PBS as previously described. 13 The sample was sonicated … When the aptamer sequences were introduced to SWCNT during sonication, they may exist in non-optimal conformations on the surface of the nanotube. We sought to assess aptamer-based sensor function in conditions which would promote optimal aptamer folding after sonication and purification. …

Germline loss-of-function variant in the E3 ubiquitin ligase TRAF2 in a young adult patient with medulloblastoma: a case report.

We identified a rare heterozygous germline loss-of-function variant in the tumor necrosis factor receptor-associated factor 2 (TRAF2) in a young adult patient diagnosed with medulloblastoma. This variant is located within the TRAF-C domain of the E3 ubiquitin ligase protein and is predicted to diminish the binding affinity of TRAF2 to upstream receptors and associated adaptor proteins. Integrative genomics revealed a biallelic loss of TRAF2 via partial copy-neutral loss-of-heterozygosity of 9q in the medulloblastoma genome. We further performed comparative analysis with an in-house cohort of 2

Downregulation of SLC40A1 leads to iron accumulation in fibrotic lung fibroblasts.

Iron is an essential nutrient for almost all organisms. However, excess iron generates reactive oxygen species and causes tissue injuries. Iron is implicated in idiopathic pulmonary fibrosis (IPF). In this study, we examined iron accumulation in fibrotic lung fibroblasts and the underlying mechanisms. We hypothesize that the downregulation of Solute Carrier Family 40 Member 1 (SLC40A1) results in iron accumulation in lung fibroblasts of IPF patients. Using a Prussian Blue iron staining, we found that iron accumulated in the fibrotic region of the lungs from IPF patients and bleomycin- and asbe

Структурное и механистическое профилирование модулятуры Hurr1 vidofludimus позволяет проектировать лиганд на основе структуры.

В центре биомедицинских исследований находится фактор нейрозащиты, связанный с ядерным рецептором 1 (Nurr1, NR4A2) для его многообещающей нейрозащитной роли в болезни Паркинсона, болезни Альцгеймера и множественном склерозе. Его деятельность может контролироваться лигандами, предлагающими доступ к фармакологической модуляции Hurr1. Вместе с тем связующие эпитопы и механизмы молекулярной активации синтетических активаторов Hurr1 по-прежнему остаются труднодостижимыми, но имеют важное значение для продвижения к новым лекарствам лигандов Hurr1. Здесь мы охарактеризовали диссоциацию Hurr1 димера и

Совпадение в полном тексте источника
… Cells were kept on ice and disrupted by sonication in the presence of 1 mM ATP, DNAse I, 10 mM MgSO 4 , EDTA-free cOmplete™ protease inhibitor cocktail (F. Hoffmann-La Roche AG, Basel, Switzerland), and lysozyme. Sonication was performed in cycles of 5 s on/10 s off at 35% for 18 min. … Cells were kept on ice and disrupted by sonication in the presence of EDTA-free cOmplete™ protease inhibitor cocktail (F. Hoffmann-La Roche AG, Basel, Switzerland). Sonication was performed in cycles of 5 s on/10 s off at 35% for 18 min. …

Molecular classification of gastric cancer: Towards a pathway-driven targeted therapy.

Gastric cancer (GC) is the third leading cause of cancer mortality worldwide. Although surgical resection is a potentially curative approach for localized cases of GC, most cases of GC are diagnosed in an advanced, non-curable stage and the response to traditional chemotherapy is limited. Fortunately, recent advances in our understanding of the molecular mechanisms that mediate GC hold great promise for the development of more effective treatment strategies. In this review, an overview of the morphological classification, current treatment approaches, and molecular alterations that have been c

Emerging agents that target signaling pathways to eradicate colorectal cancer stem cells.

Colorectal cancer (CRC) represents the third most commonly diagnosed cancer and the second leading cause of cancer death worldwide. The modern concept of cancer biology indicates that cancer is formed of a small population of cells called cancer stem cells (CSCs), which present both pluripotency and self-renewal properties. These cells are considered responsible for the progression of the disease, recurrence and tumor resistance. Interestingly, some cell signaling pathways participate in CRC survival, proliferation, and self-renewal properties, and most of them are dysregulated in CSCs, includ

Эгоистические нейрофармацевтики: открытие кровеносного барьера для лечения следующего поколения.

Блокирование мозга крови (ББД) представляет собой огромную проблему в нейрофармакологии, ограничивая доставку терапевтических агентов в мозг. Экзосомы, нанокарьеры природы, стали многообещающим решением из-за их биосовместимости, низкой иммуногенности и врожденной способности пересекать ББД. Тщательное изучение анатомии и физиологии ББД свидетельствует о сложности доставки неврологических наркотиков и подчеркивает ограниченность традиционных методов. В настоящем обзоре изучается потенциал нейрофармацевтики с экзосомной силой, при этом особое внимание уделяется их структурным и функциональным с

Совпадение в полном тексте источника
… Prominent active loading strategies include electroporation, sonication, extrusion, and emerging methods such as freeze-thaw cycles and chemical conjugation. … Sonication utilizes ultrasonic waves to temporarily disrupt the exosomal membrane, facilitating the encapsulation of therapeutic agents [ 111 ]. …

Targeted therapy for advanced Basal-cell carcinoma: vismodegib and beyond.

Basal-cell carcinoma is a commonly occurring skin malignancy that has the potential to progress into locally invasive or resistant disease, as well as spread distantly. Due to advances in the molecular understanding of the disease over the last two decades, it has been discovered that the Hedgehog pathway plays an important role in the pathogenesis of this disease and can be exploited as a treatment target. Several agents that inhibit the Hedgehog pathway have reached clinical studies and one drug, vismodegib, has recently been US Food and Drug Administration (FDA) approved based on clinical a

Nuclear Heparanase Regulates Chromatin Remodeling, Gene Expression and PTEN Tumor Suppressor Function.

Heparanase (HPSE) is an endoglycosidase that cleaves heparan sulfate and has been shown in various cancers to promote metastasis, angiogenesis, osteolysis, and chemoresistance. Although heparanase is thought to act predominantly extracellularly or within the cytoplasm, it is also present in the nucleus, where it may function in regulating gene transcription. Using myeloma cell lines, we report here that heparanase enhances chromatin accessibility and confirm a previous report that it also upregulates the acetylation of histones. Employing the Multiple Myeloma Research Foundation CoMMpass datab

Controlled Drug Delivery Systems for Oral Cancer Treatment-Current Status and Future Perspectives.

Oral squamous cell carcinoma (OSCC), which encompasses the oral cavity-derived malignancies, is a devastating disease causing substantial morbidity and mortality in both men and women. It is the most common subtype of the head and neck squamous cell carcinoma (HNSCC), which is ranked the sixth most common malignancy worldwide. Despite promising advancements in the conventional therapeutic approaches currently available for patients with oral cancer, many drawbacks are still to be addressed; surgical resection leads to permanent disfigurement, altered sense of self and debilitating physiologica

Совпадение в полном тексте источника
… Since these particles were difficult to solubilize, they were suspended in water or saline after sonication. Iron–salen particles were characterized using DLS and TEM, showing size ranged 1.2–3 µm for unsonicated particles and 60–800 nm for sonicated particles. … These nanoparticles were sonicated for 30 min and were suspended in normal saline. …

The osteogenic niche-targeted arsenic nanoparticles prevent colonization of disseminated breast tumor cells in the bone.

Up to 70% of patients with late-stage breast cancer have bone metastasis. Current treatment regimens for breast cancer bone metastasis are palliative with no therapeutic cure. Disseminated tumor cells (DTCs) colonize inside the osteogenic niches in the early stage of bone metastasis. Drug delivery into osteogenic niches to inhibit DTC colonization can prevent bone metastasis from entering its late stage and therefore cure bone metastasis. Here, we constructed a 50% DSS6 peptide conjugated nanoparticle to target the osteogenic niche. The osteogenic niche was always located at the endosteum with

Совпадение в полном тексте источника
… The w/o/w double emulsion was formed via sonication for 2 min. The As 3+ and Mn 2+ were allowed to react in the inner water phase to form As-Mn nanocrystals. Dichloromethane was removed via rotary evaporation at 40 °C. … Bone marrow contents in femurs were flushed out or removed via sonication. The metaphyseal and diaphyseal bones were separated and dried in an oven at 80 °C. …

Multiple myeloma cancer stem cells.

Multiple myeloma (MM) remains incurable despite much progress that has been made in the treatment of the disease. MM cancer stem cell (MMSC), a rare subpopulation of MM cells with the capacity for self-renewal and drug resistance, is considered to lead to disease relapse. Several markers such as side population (SP) and ALDH1+ have been used to identify MMSCs. However, ideally and more precisely, the identification of the MMSCs should rely on MMSCs phenotype. Unfortunately the MMSC phenotype has not been properly defined yet. Drug resistance is the most important property of MMSCs and contribu

Recent Advances on the Pathobiology and Treatment of Multiple Myeloma.

Worldwide experts in the field of multiple myeloma (MM) have promptly answered to the call in the Special Issue entitled "Recent advances on the pathobiology and treatment of multiple myeloma", submitting basic, translational or clinical works under the form of original article, review or perspective [...].

Lower-Risk Myelodysplastic Syndrome (MDS) Patients Exhibit Diminished Proteasome Proteolytic Activity and High Intracellular Reactive Oxygen Species (ROS) Levels.

Myelodysplastic syndromes (MDS) constitute a heterogeneous group of clonal hematopoietic stem cell disorders characterized by ineffective hematopoiesis and an elevated risk of transformation to acute myeloid leukemia (AML). Available disease-modifying treatment approaches are limited. The ineffectiveness of proteasome inhibitors (PIs) in MDS patients is currently investigated, although it is unclear whether they rapidly develop resistance to PIs or whether proteasome proteolytic activity (PPA) is constitutively lower in the hematopoietic cells of these patients, thus limiting treatment effecti

Совпадение в полном тексте источника
… Proteasome activity assay Total proteins were extracted from the cells, using sonication and incubation in a buffer solution containing 50 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), 20 mM potassium chloride (KCl), 5 mM magnesium chloride and water (MgCl 2 ·H 2 O), and 1 mM dithiothreitol … Western blot analysis Cells were lysed, using radioimmunoprecipitation assay (RIPA) buffer supplemented with phosphatase and protease inhibitors, and the cell contents were homogenized using sonication. Total protein concentration was estimated with the Bradford assay. …