Результаты поиска

Exosomes-loaded with traditional Chinese medicine constituents: a novel therapeutic avenue for aplastic anemia.

Aplastic anemia (AA) is a life-threatening blood disorder characterized by bone marrow failure and pancytopenia. Treatments such as immunosuppressive therapy (IST) and allogeneic hematopoietic stem cell transplantation (allo-HSCT) have limitations, including poor long-term remission and donor shortages. Exosomes, nanosized extracellular vesicles, are promising drug delivery systems (DDS) due to their biocompatibility, targeted delivery, and capacity to penetrate biological barriers. Traditional Chinese medicine (TCM) regulates hematopoiesis and immunity but faces challenges with delivery. This

Совпадение в полном тексте источника
… Techniques like sonication and surface modification are employed to optimize cargo encapsulation and tumor-targeting capabilities [ 25 , 26 ]. … , enabling the entry of TCM small molecules (e.g., ginsenosides or podophyllotoxin derivatives) into exosomes, while sonication disrupts lipid bilayers to improve drug-loading capacity [ 153 ]. …

Co-Encapsulation of Multiple Antineoplastic Agents in Liposomes by Exploring Microfluidics.

The inherent complexity of cancer proliferation and malignancy cannot be addressed by the conventional approach of relying on high doses of a single powerful anticancer agent, which is associated with poor efficacy, higher toxicity, and the development of drug resistance. Multiple drug therapy (MDT) rationally designed to target tumor heterogeneity, block alternative survival pathways, modulate the tumor microenvironment, and reduce toxicities would be a viable solution against cancer. Liposomes are the most suitable carrier for anticancer MDT due to their ability to encapsulate both hydrophil

Совпадение в полном тексте источника
… Pisani et al. compared different active loading methods (pH gradient, freeze–thaw method, sonication, electroporation) for the encapsulation of proteins in liposomes synthesized by means of the microfluidic technique. … The desired release behavior of the encapsulated drugs can be achieved by incorporating multifunctionality in the liposomes, such as stimuli-responsiveness to various internal (pH, reactive oxygen species (ROS), glucose levels, or enzymes) or external stimuli (temperature, sonication, or light). …

Advances in Drug Delivery and Biomaterials: Facts and Vision.

Drug delivery and biomaterials are different fields of science but, at the same time, are tightly related and intertwined. The 2018 CRS Italy Chapter Annual Workshop aims to explore recent advances in design and development in these areas. Many colleagues from Europe participated to the Workshop, stimulating the discussion. To foster the discussion on recent research and networking opportunities, especially among younger attendees, all poster-presenting authors were asked to provide a short talk. The very friendly and stimulating atmosphere allowed the attendees to explore new frontiers and ta

Совпадение в полном тексте источника
… Several pre-formulative studies were carried out (i) to drastically reduce mechanical and energetic stress factors caused by preparative procedures (e.g., sonication); (ii) to optimize the volume of liquid phases; and (iii) to choose type of surfactant and concentration. … As a method, the homogenization followed by high-frequency sonication was used. After dialysis, NLC dispersion was evaluated in terms of drug loading (DL, 2.2% w / w ) and recovery (DR, 88% w / w ). …

Targeting HDACs with dacinostat amplifies radiation-induced DNA damage via sustained phosphorylated p53 and repair inhibition in aggressive meningioma.

The lack of standard systemic treatments in meningioma presents a therapeutic challenge, making radiotherapy a crucial intervention for recurrent, high-grade, or inoperable tumours. Nonetheless, the efficacy of radiotherapy is constrained by the inherent radioresistance of meningiomas, attributed to their proficient DNA damage repair and robust cell cycle checkpoint regulation. These biological barriers, along with anatomical constraints of avoiding damage to adjacent neural tissues, restrict dose escalation and therapeutic gains. Histone deacetylases (HDACs) are mediators of radioresistance i

Совпадение в полном тексте источника
… Nuclei from 1 × 10 7 cells were isolated, extracted, sonicated for 30 s and centrifuged to yield a crude nuclear extract. Protein concentration was measured via the BCA assay kit. … Spheroids underwent three freeze-thaw cycles using liquid nitrogen and a 37 °C water bath, followed by sonication (XUBA1 Ultrasonic Bath, Grant Instruments, Cambridge, UK) for 2-min sessions twice, with a 1-min ice resting period in between. …

Recent Progress of Exosomes in Multiple Myeloma: Pathogenesis, Diagnosis, Prognosis and Therapeutic Strategies.

Multiple myeloma (MM) is a hematological malignancy that is still incurable. The bone marrow microenvironment (BMM), with cellular and non-cellular components, can create a favorable environment for the survival, proliferation and migration of MM cells, which is the main reason for the failure of MM therapies. Many studies have demonstrated that exosomes play an important role in the tumor-supportive BMM. Exosomes are nanoscale vesicles that can be released by various cells. Some exosomes contribute to the pathogenesis and progression of MM. MM-derived exosomes act on different cells in the BM

Совпадение в полном тексте источника
… Active encapsulations include electroporation [ 77 ], sonication [ 78 ], freeze and thaw cycles [ 79 ], click chemistry [ 80 ], extrusion [ 81 ] and incubation with saponin [ 82 ] ( Table 3 ). Notably, these methods result in different encapsulation rates and stability of drugs in exosomes or EMs. … Electroporation | Electrical field creates small pores in the exosome membrane, and then, drugs or nucleotides can subsequently diffuse into the interior of the exosomes via the pores. | This method can load large molecules. | This method may cause the aggregation of RNA; Low drug-loading efficiency. | [ 77 ] | Sonication …

Panobinostat reduces hypoxia-induced cisplatin resistance of non-small cell lung carcinoma cells via HIF-1α destabilization.

BackgroundLung cancer is one of the most frequent cancer types and the leading cause of cancer death worldwide. Cisplatin is a widely used chemotherapeutic for non-small cell lung carcinoma (NSCLC), however, its positive effects are diminished under hypoxia. We wanted to determine if co-treatment with cisplatin and histone deacetalyse (HDAC) inhibitor panobinostat can reduce hypoxia-induced cisplatin resistance in NSCLC cells, and to elucidate mechanism involved.MethodsExpression status of different HDACS was determined in two cell lines and in tumor tissue from 20 patients. Cells were treated

Совпадение в полном тексте источника
… (C, D) For protein analysis cryo-preserved lung tumor and non-malignant lung tissue was homogenized by blender, followed by sonication (3 x 5 seconds on ice) and centrifugation at 13000 rpm for 10 min. at 4°C in order to remove insoluble cell-debris. … Samples were homogenized by sonication performed three times for 5 seconds on ice. Protein samples were separated by SDS-PAGE and transferred onto nitrocellulose membrane (BioRad Industries, Hercules, CA, USA). …

Инженерная иммунологическая ниша направляет терапевтическое развитие в модели прогрессивного множественного склероза.

Первичный прогрессивный множественный склероз (MS) представляет собой аутоиммунное заболевание, вызывающее демиелионацию и имеющее лишь один класс лечения, одобренного УЛХ, — истощение клеток В. Новейшее лечение может возникнуть в результате более глубокого понимания взаимодействия между различными типами клеток в заболевших тканях на протяжении всего процесса развития. Первоначально мы описываем инженерную иммунологическую нишу (ИН) на основе биоматериалов как суррогатную для заболевших тканей функцию иммунных клеток и фенотипную динамику на протяжении хронической прогрессивной модели MS мыши

Совпадение в полном тексте источника
… This solution was sonicated at 100% amplitude for 30 s before adding 10 mL of 1% w/v poly(ethylene-alt-maleic anhydride) (PEMA) in water and a subsequent sonication. …

Venetoclax enhances DNA damage induced by XPO1 inhibitors: A novel mechanism underlying the synergistic antileukaemic effect in acute myeloid leukaemia.

Acute myeloid leukaemia (AML) is a highly heterogeneous haematologic malignancy with poor prognosis. We previously showed synergistic antileukaemic interaction between exportin 1 (XPO1) inhibitor KPT-330 (Selinexor) and Bcl-2 inhibitor venetoclax (ABT-199) in preclinical models of AML, which was partially meditated by Mcl-1, although the full mechanism of action remains unknown. In this study, using real-time RT-PCR and Western blot analysis, we show that inhibition of XPO1 via KPT-330 or KPT-8602 (Eltanexor) decreases the mRNA and protein levels of c-Myc, CHK1, WEE1, RAD51 and RRM2. KPT-330 a

Совпадение в полном тексте источника
… Western blot analysis AML cells were lysed by sonication in 10 mM Tris‐Cl, pH 7.0, containing 1% SDS, protease inhibitors and phosphatase inhibitors (Roche Diagnostics, Indianapolis, IN, USA). … Nuclei were resuspended and sonicated, and then subjected to Western blot analysis. Experiments were repeated three times independently. Representative blots are shown. …

NPI-0052 and γ-radiation induce a synergistic apoptotic effect in medulloblastoma.

Medulloblastoma (MB) is the most common malignant solid paediatric brain tumour. The standard treatment for MB is surgical resection of the tumour, radiation and chemotherapy. This therapy is associated with high morbidity and adverse side effects. Hence, more targeted and less toxic therapies are vitally needed to improve the quality of life of survivors. NPI-0052 is a novel proteasome inhibitor that irreversibly binds the 20S proteasome subunit. This compound has anti-tumour activity in metastatic solid tumours, glioblastoma and multiple myeloma with a good safety profile. Importantly, NPI-0

Calpastatin phosphorylation regulates radiation-induced calpain activity in glioblastoma.

Glioblastoma (GBM) is an aggressive, malignant brain tumor that inevitably develops resistance to conventional chemotherapy and radiation treatments. In order to identify signaling pathways involved in the development of radiation resistance, we performed mass spectrometry-based phospho-proteomic profiling of GBM cell lines and normal human astrocytes before and after radiation treatment. We found radiation induced phosphorylation of a number of proteins including calpastatin, specifically in GBM stem cells (GSCs). Herein, we focused on calpastatin, an endogenous inhibitor of calpain proteases

Эволюционно сохраненная созвездие функциональных резинов программирует реагирующую на вирус судьбу человека (эпи)генома.

Здоровье человека зависит от запутанной защитной клеточной реакции на микробные патогены, такие как вирусы. Несмотря на предпринятые значительные усилия, (эпи)геномические механизмы, используемые клетками человека для адаптации программ защитного генного экспрессия к микробным атакам, по-прежнему недостаточно изучены, главным образом из-за значительного отсутствия регистрации в vivo функциональных модулей резного регулирования (КРМ) генома человека. Здесь мы представляем реагирующую на вирус судьбу человека (эпи)генома, описываемого в наивных и инфицированных клетках функциональными геномами,

Совпадение в полном тексте источника
… Chromatin was isolated by sequential incubation in extraction buffers, followed by sonication. In detail, 45–60 × 10 6 cells were collected and washed twice with ice-cold 1× PBS. … Pellets were diluted in sonication Buffer (10 mM Tris-Cl, pH 8.0, 1 mM EDTA), and chromatin filaments were sheared to a range of 200–2000 bp using a Covaris S220 Sonicator, followed by two sequential steps of purification by centrifugation for 8 min at 4°C as follows: first, a low-speed round (2000 rpm …

Targeting Cancer Stem Cell Markers or Pathways: A Potential Therapeutic Strategy for Oral Cancer Treatment.

Cancer stem cells (CSCs) are a small subset of cancer cells with stem cell-like properties, self-renewal potential, and differentiation capacity into multiple cell types. Critical genetic alterations or aberrantly activated signaling pathways associated with drug resistance and recurrence have been observed in multiple types of CSCs. In this context, CSCs are considered to be responsible for tumor initiation, growth, progression, therapeutic resistance, and metastasis. Therefore, to effectively eradicate CSCs, tremendous efforts have been devoted to identify specific target molecules that play

Histone Deacetylase Inhibitors in Pediatric Brain Cancers: Biological Activities and Therapeutic Potential.

Brain cancers are the leading cause of cancer-related deaths in children. Biological changes in these tumors likely include epigenetic deregulation during embryonal development of the nervous system. Histone acetylation is one of the most widely investigated epigenetic processes, and histone deacetylase inhibitors (HDACis) are increasingly important candidate treatments in many cancer types. Here, we review advances in our understanding of how HDACis display antitumor effects in experimental models of specific pediatric brain tumor types, i.e., medulloblastoma (MB), ependymoma (EPN), pediatric

Мукоадгезивные наноэмульсии для доставки нозы в мозг диметилфумарат: разработка, характеристика и доказательство согласия

Фон. Диметилфумарат (ДМП) является утвержденным терапевтическим агентом для лечения множественного склероза, известным своими противовоспалительными и нейрозащитными свойствами. Однако его пероральное управление часто сопровождается побочными эффектами желудочно-кишечного кишечника, что ограничивает его терапевтический потенциал. В настоящем исследовании была разработана новая формула наноэмульсии (NE), которая была охарактеризована с целью усиления воздействия ДМП в цереброспинарной жидкости (CSF) после внутринационального администрирования. Методы: Состав был подготовлен методом самоэмульсиф

Совпадение в полном тексте источника
… The resulting emulsion was centrifuged at 7000 rpm for 3 min (Mini centrifuge LLG-uniCFUGE5, LLG Labware, Meckenheim, Germany), and subsequently sonicated (Sonic Dismembrator, Fisher Scientific, Waltham, MA, USA, 3 mm probe) for 18 min at 20% amplitude, with a pulsed cycle of 10 s on and 10 s off. … The final sample underwent ultrasonication (Sonic Dismembrator, Fisher Scientific, 3 mm probe) for 15 min at 20% amplitude, using a pulsed cycle of 10 s on and 10 s off. …

A Recent Review on Cancer Nanomedicine.

Cancer is one of the most prevalent diseases globally and is the second major cause of death in the United States. Despite the continuous efforts to understand tumor mechanisms and various approaches taken for treatment over decades, no significant improvements have been observed in cancer therapy. Lack of tumor specificity, dose-related toxicity, low bioavailability, and lack of stability of chemotherapeutics are major hindrances to cancer treatment. Nanomedicine has drawn the attention of many researchers due to its potential for tumor-specific delivery while minimizing unwanted side effects

Совпадение в полном тексте источника
… Several techniques, including electroporation, transfection, incubation, extrusion, sonication, thermal shock, freeze–thaw cycles, hypotonic dialysis, and pH gradient method, have been explored to load exosomes with diverse chemotherapeutic drugs and other bioactive compounds [ 175 ]. … RAW 264.7 mouse macrophage-derived exosomes were loaded with paclitaxel via incubation at room temperature, electroporation, or mild sonication, and the free drug was removed from the exoPTX by size exclusion chromatography. …

Functions and Therapeutic Potentials of Long Noncoding RNA in Skeletal Muscle Atrophy and Dystrophy.

Skeletal muscle is the most abundant tissue in the human body and is responsible for movement, metabolism, energy production and longevity. Muscle atrophy is a frequent complication of several diseases and occurs when protein degradation exceeds protein synthesis. Genetics, ageing, nerve injury, weightlessness, cancer, chronic diseases, the accumulation of metabolic byproducts and other stimuli can lead to muscle atrophy. Muscular dystrophy is a neuromuscular disorder, part of which is caused by the deficiency of dystrophin protein and is mostly related to genetics. Muscle atrophy and muscular

Indian Ayurvedic medicine: Overview and application to brain cancer.

Ayurveda is the traditional medicine system of India, and has been in practice for millennia. It is a traditional approach that uses 1000's of different plant preparations in various combinations for treatment of human ailments, including cancer. Ethnopharmacological and phytochemical analyses are now elucidating the bioactive constituents of the different plant species and herbal formulations, including ashwagandha, curcumin, guduchi, triphala, and others. To provide an overview of: 1) the ethnopharmacology of Ayurveda and several of its most important plant species and formulations, includin

Biomedical Promise of Sustainable Microwave-Engineered Symmetric Curcumin Derivatives.

Curcumin is a polyphenol of the Curcuma longa plant, which can be used for various medicinal purposes, such as inflammation and cancer treatment. In this context, two symmetric curcumin derivatives (D1-(1E,6E)-1,7-bis(4-acetamidophenyl)hepta-1,6-diene-3,5-dione and D2-p,p-dihydroxy di-cinnamoyl methane) were obtained by the microwave-based method and evaluated for their antitumoral effect on human cervix cancer in comparison with toxicity on non-tumoral cells, taking into account that they were predicted to act as apoptosis agonists or anti-inflammatory agents. The HeLa cell line was incubated

Shilajit mitigates chemotherapeutic drug-induced testicular toxicity: Study on testicular germ cell dynamics, steroidogenesis modulation, and Nrf-2/Keap-1 signaling.

BACKGROUND: Medications, including chemotherapeutic drugs, contribute to male infertility as external factors by inducing oxidative stress in testicular cells. Shilajit is a naturally occurring bioactive antioxidant used in Ayurvedic medicine to treat a variety of ailments. OBJECTIVE: This study examines the potential of Shilajit to counteract the negative effects of the chemotherapeutic drug cyclophosphamide (CPA) on testicular germ cell dynamics. MATERIAL AND METHODS: Male Parkes mice received single intraperitoneal CPA injection (200 mg/kg BW) on day one, followed by daily supplementation

Совпадение в полном тексте источника
… Daily sperm production (DSP) To evaluate the effects of Shilajit supplementation on DSP in CPA-exposed mice, elongated spermatids (step 14–16 spermatids) resistant to sonication were counted according to Meistrich and van Beek (1993). … In brief, one testis was homogenized in 1ml of chilled distilled water and sonicated for 1.5 min. The sample was counted in a haemocytometer under a phase contrast microscope at 400X magnification. In mice spermatogenesis, developing spermatids spend 4.84 days in step 14–16. …

RXR Agonists Enhance Lenalidomide Anti-Myeloma Activity and T Cell Functions while Retaining Glucose-Lowering Effect.

Retinoid X receptor (RXR) heterodimerizes with the PPAR nuclear hormone receptor and regulates its downstream events. We investigated the effects of RXR agonists (LG100754, bexarotene, AGN194204, and LG101506) on lenalidomide's anti-myeloma activity, T cell functions, and the level of glucose and lipids in vivo. Genetic overexpression and CRISPR/Cas9 knockout experiments were conducted in multiple myeloma (MM) cell lines and Jurkat T cell lines to determine the roles of CRBN in RXR-agonist mediated effects. A xenograft mouse model of MM was established to determine the combination effect of LG

Совпадение в полном тексте источника
… The cells were lysed via brief sonication and then spun down to remove the cell debris. The total protein in the cell lysates was quantified using a Dc protein estimation kit (Bio-Rad, Hercules, CA, USA) with bovine serum albumin (BSA) as a standard. … The resulting pellets were sonicated, and the chromatin solution was precleared with 30 μL of ChIP-grade protein G magnetic beads (#9006; Cell Signaling Technology, Danvers, MA, USA). …