Результаты поиска

Role of endothelins on lymphocyte accumulation in allergic pleurisy.

Endothelins participate in different aspects of inflammatory reactions, including edema formation and eosinophil accumulation in allergic reaction. In this study, we demonstrated a role for endogenous endothelins in eosinophil and T lymphocyte recruitment and cytokine secretion in a murine model of allergic inflammation. Intrathoracic stimulation with endothelin-1 triggered a neutrophil accumulation at 4 h, concomitant with an increase of CD4+ and CD8+ T lymphocyte populations. Antigen challenge in sensitized animals leads to an increase in eosinophil and mononuclear cell numbers at 24 h. Trea

Differences in Type 1 and Type 2 intracytoplasmic cytokines, detected by flow cytometry, according to immunosuppression (cyclosporine A vs. tacrolimus) in stable renal allograft recipients.

Recent multicenter, randomized clinical trials have shown that in renal transplant patients tacrolimus (FK506) was more efficient than cyclo … od mononuclear cells (PBMCs) with phorbol myristate acetate (PMA) and ionomycin, in the presence of monensin, in 14 healthy volunteers (controls) and in 14 renal transplant patients. The immunosuppression of the latter was based either on CsA (n = 7) or on FK506 (n = 7). Cytokine-expressing T-cell frequencies were assessed immediately pretransplantation (D …

Degradation of radioiodinated B cell monoclonal antibodies: inhibition via a FCgamma-receptor-II-mediated mechanism and by drugs.

Our aim is to treat patients with B cell malignancies with radioimmunotherapy using monoclonal antibodies (mAb) such as CD19, CD20 and CD22. In this study we investigated the rate of internalization and catabolism of these mAb. After 24 h at 37 degrees C, 20%-25% of initially cell-bound (125)I-CD19 mAb and (125)I-CD22 mAb was degraded in B cells, whereas almost no degredation occurred after binding of (125)I-CD20 mAb. For B cells expressing Fcgamma receptor II (FcgammaRII), isotype-dependent degradation was noted as the CD19 IgGl mAb showed an enhanced degradation rate compared to the switch v

Inhibitors of clathrin-dependent endocytosis enhance TGFbeta signaling and responses.

Clathrin-dependent endocytosis is believed to be involved in TGFbeta-stimulated cellular responses, but the subcellular locus at which TGFbeta induces signaling remains unclear. Here, we demonstrate that inhibitors of clathrin-dependent endocytosis, which are known to arrest the progression of endocytosis at coated-pit stages, inhibit internalization of cell-surface-bound TGFbeta and promote colocalization and accumulation of TbetaR-I and SARA at the plasma membrane. These inhibitors enhance TGFbeta-induced signaling and cellular responses (Smad2 phosphorylation/nuclear localization and expres

Induction of autophagy promotes fusion of multivesicular bodies with autophagic vacuoles in k562 cells.

Morphological and biochemical studies have shown that autophagosomes fuse with endosomes forming the so-called amphisomes, a prelysosomal hybrid organelle. In the present report, we have analyzed this process in K562 cells, an erythroleukemic cell line that generates multivesicular bodies (MVBs) and releases the internal vesicles known as exosomes into the extracellular medium. We have previously shown that in K562 cells, Rab11 decorates MVBs. Therefore, to study at the molecular level the interaction of MVBs with the autophagic pathway, we have examined by confocal microscopy the fate of MVBs

Revolutionizing ovarian cancer therapy by drug repositioning for accelerated and cost-effective treatments.

Drug repositioning, the practice of identifying novel applications for existing drugs beyond their originally intended medical indications, stands as a transformative strategy revolutionizing pharmaceutical productivity. In contrast to conventional drug development approaches, this innovative method has proven to be exceptionally effective. This is particularly relevant for cancer therapy, where the demand for groundbreaking treatments continues to grow. This review focuses on drug repositioning for ovarian cancer treatment, showcasing a comprehensive exploration grounded in thorough in vitro

Совпадение в полном тексте источника
… Monensin is primarily used as a veterinary antibiotic and feed additive for livestock, especially in the prevention and control of coccidiosis. The use of Monensin in human medicine is not authorized, and it is not prescribed for human consumption. … Monensin was shown to regulate the expression molecules linked to the epithelial-mesenchymal transition (EMT) and the mitogen-activated protein kinase (MEK)-extracellular signal-regulated kinase (ERK) pathway ( 61 ). …

Drug Repurposing Opportunities in Pancreatic Ductal Adenocarcinoma.

Pancreatic ductal adenocarcinoma (PDAC) is considered one of the deadliest tumors worldwide. The diagnosis is often possible only in the latter stages of the disease, with patients already presenting an advanced or metastatic tumor. It is also one of the cancers with poorest prognosis, presenting a five-year survival rate of around 5%. Treatment of PDAC is still a major challenge, with cytotoxic chemotherapy remaining the basis of systemic therapy. However, no major advances have been made recently, and therapeutic options are limited and highly toxic. Thus, novel therapeutic options are urgen

Совпадение в полном тексте источника
… Given its toxicity, monensin is not used in humans; however, it has been studied for drug repurposing in different types of malignancies, including pancreatic cancer [ 4 ]. … In PDAC xenograft mouse models, monensin impaired tumor growth via the EGFR pathway [ 65 ]. 3.1.5. …

Tripchlorolide induces autophagy in lung cancer cells by inhibiting the PI3K/AKT/mTOR pathway and improves cisplatin sensitivity in A549/DDP cells.

Tripchlorolide (T4) has been shown to induce A549 lung cancer cell death predominantly by activating an autophagy pathway. However, the underlying mechanism remains unclear. Herein, we demonstrated that compared with T4 treatment alone, pretreatment with wortmannin (an inhibitor of phosphatidylinositol 3-kinase), perifosine (an inhibitor of AKT) or rapamycin (an inhibitor of mTOR) combined with a subsequent T4 treatment significantly impaired the cell viability of A549 and A549/DDP lung cancer cells. We found that either treatment scheme markedly reduced the activity of P13K and AKT. Expressio

Совпадение в полном тексте источника
… 56 24264886 10.1007/s10495-013-0918-3 7 Meng Y Wang L Chen D Chang Y Zhang M Xu JJ Zhou R Zhang QY LAPTM4B: an oncogene in various solid tumors and its functions Oncogene 2016 35 6359 27212036 10.1038/onc.2016.189 PMC5161753 8 Choi HS Jeong EH Lee TG Kim SY Kim HR Kim CH Autophagy inhibition with monensin … enhances cell cycle arrest and apoptosis induced by mTOR or epidermal growth factor receptor inhibitors in lung cancer cells Tuberc Respir Dis (Seoul) 2013 75 9 17 23946753 10.4046/trd.2013.75.1.9 PMC3741474 9 Choi HS Jeong EH Lee TG Kim SY Kim HR Kim CH Autophagy inhibition with monensin enhances …

Enumeration, functional responses and cytotoxic capacity of MAIT cells in newly diagnosed and relapsed multiple myeloma.

Mucosal-associated invariant T (MAIT) cells are T cells that recognise vitamin-B derivative Ag presented by the MHC-related-protein 1 (MR1) antigen-presenting molecule. While MAIT cells are highly abundant in humans, their role in tumour immunity remains unknown. Here we have analysed the frequency and function of MAIT cells in multiple myeloma (MM) patients. We show that MAIT cell frequency in blood is reduced compared to healthy adult donors, but comparable to elderly healthy control donors. Furthermore, there was no evidence that MAIT cells accumulated at the disease site (bone marrow) of t

Совпадение в полном тексте источника
… /v) Penicillin (100 U/ml), Streptomycin (100 μg/ml), Glutamax (2 mM), sodium pyruvate (1 mM), nonessential amino acids (0.1 mM), HEPES buffer (15 mM), pH7.2-7.5 (all from Invitrogen, Life Technologies) and 2-mercaptoethanol (50 μM, Sigma)) in the presence of 10ng/ml PMA, 1 μg/ml ionomycin and 1/500 Monensin … In the final 6 hr of culture, monensin (1:1500) and Brefeldin A (1:1000; both BD Biosciences) were added to the cultures. Cells were then harvested and stained as above. For Len/ E. coli stimulation assays, no monensin was added. …

Сердечно-сосудистые заболевания

Аннотация не предоставлена источником. Откройте карточку для перехода к публикации.

Совпадение в полном тексте источника
… Abnormal echocardiographic findings have been reported during experimental intoxication with monensin sodium by Varga and coworkers. … Decreased cardiac chamber size and altered left ventricular function were evident from 48 to 120 hours after administration when monensin was fed daily at approximately 25 to 50 times therapeutic levels. …

Безопасность и эффективность добавки корма, состоящей из Enterococcus lactisNICIMB 10415 (Cylactin) для всей птицы, предназначенной для жирения или выращивания для слоения/бридинга и декоративных птиц, для всех суидов, телят и других видов червей, предназначенных для сжижения или выращивания (DSM Dutal Products Ltd).

По просьбе Европейской комиссии ЕФСА было предложено представить научное заключение по оценке Цилактин в качестве зоотехнической добавки. Активным агентом присадки является Enterococcus lactis NCIMB 10415, и three Разрешенные в настоящее время составы присадок: Cylactin (LBC G35), Cylactin (LBC ME10) и Cylactin (LBC ME20) плюс. В настоящее время эта добавка разрешена в ЕС для использования в птице (цыплятах и мелких видах птицы для жиров, цыплят и мелких видов, выращиваемых для стирания), телятах и детях для выращивания и жирения, свиней, кормовых и откормленных поросят и свиней для откорма. В

Совпадение в полном тексте источника
… The use is permitted in feed containing the authorised coccidiostats: decoquinate, monensin sodium, robenidine hydrochloride, diclazuril or semduramycin. Chickens reared for laying | ME10 ME20 plus | 1. … The use is permitted in feed containing the authorised coccidiostats: monensin sodium, diclazuril, lasalocid A sodium or salinomycin sodium. Minor poultry species for fattening and reared for laying | ME10 ME20 plus | 1. …

Blockage of Autophagy for Cancer Therapy: A Comprehensive Review.

The incidence and mortality of cancer are increasing, making it a leading cause of death worldwide. Conventional treatments such as surgery, radiotherapy, and chemotherapy face significant limitations due to therapeutic resistance. Autophagy, a cellular self-degradation mechanism, plays a crucial role in cancer development, drug resistance, and treatment. This review investigates the potential of autophagy inhibition as a therapeutic strategy for cancer. A systematic search was conducted on Embase, PubMed, and Google Scholar databases from 1967 to 2024 to identify studies on autophagy inhibito

Совпадение в полном тексте источника
… Monensin, Protein Source and Protein Levels for Growing Steers J. Anim. Sci. 1979 48 474 479 10.2527/jas1979.483474x 120. Markowska A. Kaysiewicz J. Markowska J. Huczyński A. Doxycycline, Salinomycin, Monensin and Ivermectin Repositioned as Cancer Drugs Bioorg. Med. Chem. … Monensin Induces PC-3 Prostate Cancer Cell Apoptosis via ROS Production and Ca2+ Homeostasis Disruption Anticancer Res. 2016 36 5835 5844 10.21873/anticanres.11168 27793906 122. Miura K. Kawano S. Suto T. Sato T. Chida N. Simizu S. …

Сердечно-сосудистые заболевания

Аннотация не предоставлена источником. Откройте карточку для перехода к публикации.

Совпадение в полном тексте источника
… Toxins Ionophores such as monensin and lasolocid are capable of damaging myocardial and skeletal muscle when ingested in toxic amounts. Improper mixing of ionophores into rations is the most common error that may lead to toxicity, but accidental exposure to concentrated products also is possible. …

< < Элотузумаб > > CD16-Independent NK Спутниковая цитотоксичность против ячеек миеломы путем регулирования нескольких NK-подкрепляющих генов

Множественная миелома (ММ) — неразрешимая гематологическая порча, вызванная аномалиями в плазменных клетках. Комбинированная терапия с использованием антител и эффектов естественного убийцы (НК), которые являются врожденными иммунными клетками с безопасной и мощной антитумбурной активностью, является перспективным подходом к онкологической иммунной терапии и может усилить антитумберные эффекты. Elotuzumab (Elo) — антитело с иммунной оптимистикой, предназначенное для семейства молекул лимфоцитной активации. 7 (SLAMF7) на поверхности клеток MM и NK. Мы подтвердили, что Эло активно пропагандирует

Совпадение в полном тексте источника
… Purified isotype IgG1 mAb (#401402), anti-human PE-conjugated anti-CD319 (#331806) and anti-CD279 (PD-1, #367403), PE/Cy7-conjugated anti-CD16 (#302016) antibodies, and monensin solution (#420701) were purchased from BioLegend (San Diego, CA, USA). … Next, an inhibitor monensin was added (1 : 1000) and the cells were incubated for an additional 3 hr. …

Akkermansia моноколонизация модифицирует микроглию и астроциты конкретным штаммом.

Микроглиа и астроциты являются основными глиальными клетками центральной нервной системы (КНС), и их функция определяется множеством факторов. Поступили сообщения о регулировании CNS glia микробиотой, хотя роль конкретных бактерий не была определена. Мы колонизировали мышей, свободных от микробов, с штаммом Akkermansia muciniphila (AmT) и романом A. muciniphila штамм BWH-H3 (Am-H3), изолированным от предмета с множественным склерозом, и по сравнению с мышами, колонизированными с штаммом BWH-E5 (Bc), изолированными от здорового контрольного предмета. Затем мы изучили влияние этих бактерий на ми

Совпадение в полном тексте источника
… Splenocytes for cytokine analysis were stimulated at 37 °C for 4 h with PMA/ionomycin and GolgiStop containing monensin (1:1000; BD) prior to being centrifuged at 1500 rcf for 5 min and decanted. …

Биоразлагаемый электроспун PLGA Nanofibers-Encapsolated Trichinella Sphiralis Antigens защищает от повторного использования экспериментального аутоиммунного энцефаломилита и связанного с ним микробиота дисбиоз.

ЦЕЛЬ: " Тричинелла спиралис " создала сложные механизмы иммуномодуляции при посредничестве экскременто-секретарских продуктов (ESL1), которые позволяют ей выжить в принимающей стране. Следовательно, антигены ESL1 обладают прекрасным потенциалом для лечения аутоиммунных заболеваний, таких, как множественный склероз (MS). Однако вопрос о том, могут ли своевременные контролируемые поставки антигенов ESL1 в vivo, как и в случае естественных инфекций, повысить его терапевтический потенциал в отношении MS, до сих пор неизвестен.Для этого мы ввели антигены ESL1 в полихлорированные (лактидно

Совпадение в полном тексте источника
… Intracellular staining of IFN-γ, IL-10 and IL-17 was performed after 6-hour activation of the cell with phorbol-12-myristate-13-acetate (PMA, 20  ng/mL) and ionomycin (500  ng/mL) in the presence of monensin (2 µM) (all from Sigma-Aldrich). … Seven days before the immunisation, animals were implanted with either PLGA/ESL1 or sham-operated, as described, and then isolated from CNS and activated with PMA (20ng/mL) /Ca Ionophore (500ng/mL) and Monensin 3 µM for 4h before staining for flow cytometry. …

Targeting Autophagy with Natural Products as a Potential Therapeutic Approach for Cancer.

Macro-autophagy (autophagy) is a highly conserved eukaryotic intracellular process of self-digestion caused by lysosomes on demand, which is upregulated as a survival strategy upon exposure to various stressors, such as metabolic insults, cytotoxic drugs, and alcohol abuse. Paradoxically, autophagy dysfunction also contributes to cancer and aging. It is well known that regulating autophagy by targeting specific regulatory molecules in its machinery can modulate multiple disease processes. Therefore, autophagy represents a significant pharmacological target for drug development and therapeutic

Совпадение в полном тексте источника
… Docking and Fusion Stage Inhibitors Polyether ionophores, such as monensin and nigericin, are produced by Streptomyces species and are potent antimicrobial and anticancer agents that belong to a large class of naturally occurring polyketides [ 76 ]. … A number of studies have revealed that monensin and nigericin have been linked to autophagy and cell death via the interference from the fusion of autophagosome and lysosome, and thus blocks the maturation of the autophagic process. …

Короткоцепные жирные кислоты в множестве склероза: связаны с инвалидностью, количество повреждений Т2 и провокационный профиль.

ЦЕЛЬ: Произошло изменение состава кишечного микробиота у пациентов с множественным склерозом (pwMS) в том, что касается здорового контроля (HC). Было предложено, чтобы метаболиты микроорганизмов, такие, как короткоцепные жирные кислоты (СКФА), сыграли определенную роль в этой болезни. Таким образом, проанализировать связь СКФА с клиническими и радиологическими параметрами заболевания и с теми, которые связаны с подстрекательской реакцией иммунной системы. МЕТОДЫ: Многоцентральное ретроспективное исследование по перекрестному сечению. Кроме того 161 ПТМС и 130 В него были включены НС. С помощью

Совпадение в полном тексте источника
… Louis, MO), in the presence of 2 μg/mL Brefeldin A and 2.1 μM Monensin (BD Biosciences), during 4 h. After incubation, PBMC were washed with PBS and stained for 30 min at 4°C in the dark with appropriate amounts of monoclonal antibodies recognizing the surface antigens (Table  S1 ). …

Exogenous α-ketoglutarate Modulates Redox Metabolism and Functions of Human Dendritic Cells, Altering Their Capacity to Polarise T Cell Response.

Alpha-ketoglutarate (αKG) emerged as a key regulator of energetic and redox metabolism in cells, affecting the immune response in various conditions. However, it remained unclear how the exogenous αKG modulates the functions of dendritic cells (DCs), key cells regulating T-cell response. Here we found that non-toxic doses of αKG display anti-inflammatory properties in human APC-T cell interaction models. In a model of monocyte-derived (mo)DCs, αKG impaired the differentiation, and the maturation of moDCs induced with lipopolysaccharide (LPS)/interferon (IFN)-γ, and decreased their capacity to

Совпадение в полном тексте источника
… To detect intracellular cytokines in T cells, the same co-cultures were treated with PMA/ionomycin and monensin (2 μM, Sigma-Aldrich) for the last 4 h, and then prepared for the flow cytometry analysis. … D) A representative analysis of intracellular cytokines is shown, within CD8 + and CD4 + (CD8 - ) T cells co-cultured with mature moDCs (mDCs) as in (C) and stimulated with PMA/ Ca ionophore/ monensin for the last 4h of cultures. …