Результаты поиска

Combining advanced 3D spheroid-based skin models with deep-learning-based image analysis enables in-depth investigation of keratinocyte differentiation and barrier function.

To date, organotypic skin models represent the gold standard for preclinical dermatological and toxicological studies. However, they are variable in quality and require long maturation times and many cells, mainly of primary origin. We propose dermal-epidermal spheroids as an alternative model that balances the physiological relevance and throughput. Alongside the corresponding full thickness skin models, three different fibroblast/keratinocyte coculture spheroids were generated. These studies used the commonly employed HaCaT cells as well as two recently immortalized keratinocyte cell lines, …

Molecular Profiling and Selective Pro-Apoptotic Activity of a Pruning-Derived Lavandula dentata Leaf-Surface Extract in Colorectal Cancer Cells.

Lavandula dentata is a medicinal and aromatic plant rich in specialised secondary metabolites, but the biomedical potential of leaf-surface … ity in a concentration-dependent manner, with an IC50 of 27.8 ± 1.049 μg/mL, whereas MDA-MB-231 and HaCaT cells were less sensitive. Mechanistic analyses in HCT116 cells showed increased early and late apoptotic populations, mitochondrial membrane depolarisation, and enhanced cleavage of caspase-9, caspase-3, and PARP. These findings indicate that a chemicall …

Antineoplastic Activity of Podophyllotoxin and Juniper Extracts Encapsulated in MPEG-b-PLA Diblock Copolymer Micelles in Cutaneous Squamous Carcinoma Cells.

Nanotechnology offers alternative approaches to the discovery of anticancer drugs. Hydrophobic bioactive components can be included in the c … assessment, reactive oxygen species, and apoptosis induction by the loaded nanocarriers in A-431 or HaCaT cells were analyzed by the comet assay, FACS, Hoechst DNA staining, Laurdan generalized polarization, and other methods. As a result of various cellular processes induced by the PPT component-loaded nanoparticles, effector caspase-3 and caspase-7 activati …

Functional Characterisation of Surfactant Protein A as a Novel Prophylactic Means against Oncogenic HPV Infections.

Human papillomavirus (HPV) infection poses a significant health challenge, particularly in low- and middle-income countries (LMIC), where li … l accumulation in macrophages and HaCaT keratinocytes but also resulted in a decreased infection of HaCaT cells, which was further decreased when co-cultured with innate immune cells. An analysis of human innate immune cell cytokine profiles revealed a significant inflammatory response upon SP-A exposure, potentially contributing to the overall inhibition of …

PepFect14 mediates the delivery of mRNA into human primary keratinocytes and in vivo.

mRNA-based vaccines and candidate therapeutics have great potential in various medical fields. For the delivery of mRNA into target cells an … athways, we show that PF14-mRNA nanoparticles primarily entered HeLa cells via macropinocytosis and HaCaT cells via both macropinocytosis and clathrin-mediated endocytosis, while none of the blockers significantly affected the delivery into primary keratinocytes. Finally, subcutaneous injection of PF14-mRNA nanoparticles before inducing mouse irritant contact …

Psidium guajava L. hydroethanolic extract as endodontic irrigant: phytochemical analysis, antioxidant activity, antimicrobial action and biocompatibility.

BACKGROUND: The search for novel antimicrobial agents in Endodontics is constant to overcoming persistent infections. Psidium guajava L. is a medicinal plant little explored in Endodontics. The aim of this study was to produce hydroethanolic extract of P. guajava L. and to evaluate its phytochemical composition, antimicrobial and antibiofilm action against standard and clinical strains of Enterococcus faecalis and Candida albicans, and cytotoxicity and genotoxicity on human keratinocyte cultures (HaCaT cells). The findings provide new insights into the potential of P. guajava as an alternative…

Qingxiong Ointment and its Active Ingredient, Shikonin Treat Psoriasis through HIF-1 Signaling Pathway.

BACKGROUND: Psoriasis is a common chronic inflammatory skin disorder. Qingxiong ointment (QX) is a natural medicinal combination frequently employed in clinical treatment of psoriasis. However, the active ingredients of QX and its precise mechanisms of improving psoriasis remain unclear. This study elucidated the effects of QX on an Imiquimod (IMQ)-induced mouse model of psoriasis while also exploring the regulation of the active ingredient of QX, shikonin, on the HIF-1 signaling pathway in HaCaT cells. METHODS: A mouse model of psoriasis was established through topical application of IMQ, an…

SIRT6 Aggravates Psoriasiform Skin Inflammation Through Modulation of the SOCS2-STAT1/3 Pathway.

PURPOSE: To investigate the role and molecular mechanisms of the deacetylase SIRT6 in psoriasis and evaluate its potential as a therapeutic target for topical treatment. METHODS: The expression of SIRT6 in psoriatic lesions and healthy skin was analyzed using datasets from the GEO database. In vitro, Human Immortalized Epidermal Cells (HaCaT) were stimulated with TNF-α to establish a psoriasis-like cellular model. SIRT6 was overexpressed or knocked down in HaCaT cells to evaluate its effects on keratinocyte inflammation, proliferation, and migration. Integrated transcriptomic analysis and sub…

Syringic acid suppresses Cutibacterium acnes-induced inflammation in human keratinocytes via regulating the NLRP3/caspase-1/IL-1β signaling axis by activating PPARγ/Nrf2-antioxidant pathway.

BACKGROUND: Our previous studies have demonstrated a strong relationship betweenCutibacterium acnes(C. acnes), oxidative stress, and acne in … cell apoptosis, ROS, CAT, SOD, and other inflammatory variables in the heat-killed C. acnes-treated HaCaT cells. Next, to induce mice with acne inflammation, ICR mice were given an intradermal injection of live C. acnes into their right ears. The effect of SA on this inflammation was then examined. Moreover, we explored the mechanism of SA on PPARγ/Nrf2 and N …

Усовершенствованная последовательность транскриптомов и искусственный интеллект/машинное обучение (AI/ML) в имитируемом псориазом воспалении кератиноцитов человека.

ВВЕДЕНИЕ: Несмотря на то, что модель Keratinocyte HaCAT использовалась в предыдущих исследованиях для изучения воздействия антипсориатических агентов, по-прежнему отсутствует полное понимание механизма имитируемого имимодом распространения и трансдукции сигналов в псориазных кератиноцитах. ЦЕЛИ: Данное исследование было направлено на изучение молекулярных механизмов и путей, связанных с воспалением, подобным псориазу, в результате IMQ в кератиноцитах человека. МАТЕРИАЛЫ И МЕТОДЫ: Клетки HACAT подвергались воздействию различных концентраций IMQ, чтобы вызвать воспаление, аналогичное тому, котор

Совпадение в аннотации оригинала
INTRODUCTION: Although the HaCaT keratinocyte model has been used in previous research to study the effects of antipsoriatic agents, there i … ated with psoriasis-like inflammation caused by IMQ in human keratinocytes. MATERIALS AND METHODS: HaCaT cells were exposed to different concentrations of IMQ to induce inflammation similar to that observed in psoriasis. Cell viability was evaluated using the MTT assay and cell morphology was examined using phase-contrast microscopy. Gene expression profiles …

Differential Effects of Histidine and Histidinamide versus Cysteine and Cysteinamide on Copper Ion-Induced Oxidative Stress and Cytotoxicity in HaCaT Keratinocytes.

Metal chelators are used for various industrial and medical purposes based on their physicochemical properties and biological activities. In … SO4-induced ROS production, glutathione oxidation, lipid peroxidation, and protein carbonylation in HaCaT cells, whereas cysteine and cysteinamide had no such effects. Bovine serum albumin (BSA) showed copper-chelating activity at 0.5-1.0 mM (34-68 mg mL-1). Histidine, histidinamide, and BSA at 0.5-1.0 mM enhanced the viability of cells exposed to CuCl2 or Cu …

Polar microalgae extracts protect human HaCaT keratinocytes from damaging stimuli and ameliorate psoriatic skin inflammation in mice.

BACKGROUND: Polar microalgae contain unique compounds that enable them to adapt to extreme environments. As the skin barrier is our first li … eater ability to scavenge free radicals than did non-polar microalgae and enhanced the viability of HaCaT cells, a human keratinocyte cell line, following exposure to UVB radiation or psoriatic cytokines. These extracts also reduced barrier integrity damage and decreased mRNA levels of inflammatory cytokines in psoriatic HaCaT cells. Treatment with KSF0041 ex …

Therapeutic effects of myricetin on atopic dermatitis in vivo and in vitro.

BACKGROUND: Myricetin (Myr) is a flavonoid compound that exist widely in many natural plants. Myr has been proven to have multiple biologica … ) and ears of the mice were collected for mechanistic study. In addition, TNF-α and IFN-γ-activated HaCaT cells were used to investigate the underlying mechanism. RESULTS: Our data demonstrated that Myr alleviated the symptoms of AD by exerting anti-inflammatory and anti-allergic functions in vivo. We found that Myr treatment suppressed ear swelling and IgE …

Overexpression of Wilms' tumor 1 in skin lesions of psoriasis is associated with abnormal proliferation and apoptosis of keratinocytes.

Psoriasis vulgaris (PV) is a chronic inflammatory skin disease, which is characterized by the abnormal proliferation and apoptosis of keratinocytes. Previous studies have demonstrated that transcription factor Wilms' tumor 1 (WT1) is involved in a number of pathophysiological processes, including organ development, tumorigenesis and cell proliferation. However, the role of WT1 in PV remains unclear. In the present study, WT1 expression was analyzed by reverse transcription‑quantitative polymerase chain reaction and western blot analyses. WT1 was stably overexpressed or inhibited in HaCaT cells…

Protective effect of Juglans regia L. against ultraviolet B radiation induced inflammatory responses in human epidermal keratinocytes.

BACKGROUND: Juglans regia L. has a history of traditional medicinal use for the treatment of various maladies and have been documented with … on, prevented ROS generation, lipid peroxidation and restored the activity of antioxidant status in HaCaT cells. Moreover, MEJR pretreatment significantly prevented UVB activated inflammatory markers like TNF-α, IL-1, IL-6, NF-κB, COX-2 in HaCaT. CONCLUSION: The present findings suggest that MEJR exhibit photoprotective effects and hence it may be useful for …

Repurposed Drugs Celecoxib and Fmoc-L-Leucine Alone and in Combination as Temozolomide-Resistant Antiglioma Agents-Comparative Studies on Normal and Immortalized Cell Lines, and on C. elegans.

Glioblastoma multiforme therapy remains a significant challenge since there is a lack of effective treatment for this cancer. As most of the examined gliomas express or overexpress cyclooxygenase-2 (COX-2) and peroxisome proliferator-activated receptors γ (PPARγ), we decided to use these proteins as therapeutic targets. Toxicity, antiproliferative, proapoptotic, and antimigratory activity of COX-2 inhibitor (celecoxib-CXB) and/or PPARγ agonist (Fmoc-L-Leucine-FL) was examined in vitro on temozolomide resistant U-118 MG glioma cell line and comparatively on BJ normal fibroblasts and immortalize

Molecular signaling of the HMGB1/RAGE axis contributes to cholesteatoma pathogenesis.

UNLABELLED: Cholesteatoma represents progressive expansion of the keratinizing squamous epithelium in the middle ear with subsequent chronic … 7/27 (26 %) of normal skin specimens were weakly positive for RAGE. Proliferation and migration of HaCaT cells incubated with HMGB1 were up-regulated (p < 0.05). HMGB1 also prevented HaCaT cell apoptosis and induced activation of several molecular signaling pathways in keratinocytes. The data suggest that in cholesteatoma, HMGB1 released from stressed or nec …

Essential Oil of Matricaria chamomilla Alleviate Psoriatic-Like Skin Inflammation by Inhibiting PI3K/Akt/mTOR and p38MAPK Signaling Pathway.

BACKGROUND: The traditional Matricaria chamomilla L. has been used to treat dermatitis for thousands of years. Due to emerging trends in alt … as correlated with downregulating PI3K/Akt/mTOR and p38MAPK pathways activation mediated by MCEO in HaCaT cells treated with IL-22/TNF-α/LPS. Skin inflammation was evaluated based on the PASI score, HE staining, and relative inflammatory cytokine levels. The results showed that MCEO could significantly contribute to inflammatory skin disease treatment. CONCL …

Kuwanon A from Morus alba L. Alleviates H2O2-Induced Oxidative Damage in HaCaT Keratinocytes by Inhibiting Ferroptosis and Enhancing Antioxidant Capacity.

The root bark of Morus alba L. is commonly used as a natural antioxidant; however, its active constituents and underlying molecular mechanisms remain unclear. In this study, a bioactivity-guided isolation approach was employed to identify antioxidant substances from the root bark of Morus alba L. and to investigate their protective effects against oxidative damage in HaCaT cells. Using techniques such as silica gel column chromatography and semi-preparative HPLC, combined with NMR and HR-ESI-MS analysis, 22 compounds were isolated and identified from the dichloromethane extract of Morus alba L…

Assessment of antifungal and anti-biofilm potential of essential oil active constituents alone and in combinatorial mode of limonene and linalool against Candida albicans and Candida tropicalis.

Opportunistic Candidiasis is rising among immunocompromised and elderly patients, with increasing antifungal resistance. This study evaluate … kidney (HEK-293) and human keratinocyte (HaCaT) cell lines. A co-culture model of Candida spp. and HaCaT cells was developed to study host-pathogen interactions and antifungal efficacy. Results highlight EO-ACs, especially limonene and linalool, as promising alternatives against resistant Candida infections.