Результаты поиска

Molecular and serological evidence of spotted fever group rickettsiae and Borrelia co-infection in Ixodes ricinus and humans in north-eastern Poland.

Tick-borne spotted fever group (SFG) rickettsioses are considered emerging vector-borne infectious diseases with potentially severe consequences for public health, particularly in the temperate regions of Europe. The aim of the study was to determine the prevalence and the diversity of Rickettsia spp. in questing Ixodes ricinus ticks in north-eastern Poland, evaluate the serological evidence of exposure to SFG rickettsiae among adult residents of this region, as well as to determine the level of SFG rickettsiae co-infection with Borrelia spirochaetes in ticks and humans. In a total of 4090 tic…

Changes in composition and concentration of differential metabolites in root exudates are associated with aluminum-tolerance of Ricinus communis under a high CO2 environment.

Root exudates are the most direct performance for plants responding to adverse environments, and are also important media for materials exchange, energy transmission and information communication between the roots and rhizosphere. However, how plant roots and exudates respond to aluminum (Al) stress under elevated CO2 concentration (eCO2) is still unclear. Ricinus communis is a famous oilseed crop throughout the world, which has strong tolerance to metal contaminated soil. In the present study, root physiological changes and the exudates of this species under aluminum stress and eCO2 based on …

Evaluation of molluscicidal activity on Biomphalaria glabrata (Say, 1818) and phytochemical characterization of hydroalcoholic extract of leaves of Ricinus communis L. (EUPHORBIACEAE).

Schistosomiasis is a parasitic infection of great prevalence worldwide, affecting 250 million people in 78 countries. Faced with this problem, studies that seek to analyze molluscicidal activity from plant extracts have stood out. The present work aimed to obtain the phytochemical characterization and investigate the molluscicidal activity in the hydroalcoholic extract of Ricinus communis leaves on Biomphalaria glabrata. The hydroalcoholic extract was prepared by macerated with solvent ethanol P.A 96%, followed by filtration and concentration in rotary evaporator. Next, five groups of snails w…

Exposure of dogs and cats to Borrelia miyamotoi infected Ixodes ricinus ticks in urban areas of the city of Poznań, west-central Poland.

Borrelia miyamotoi is an emerging human pathogen that causes a relapsing fever-like disease named B. miyamotoi disease. The bacterium belongs to the relapsing fever borreliae, and similar to spirochetes of the Borrelia burgdorferi sensu lato group, it is transmitted only by hard ticks of the Ixodes ricinus complex. To date, B. miyamotoi has not been demonstrated to cause illness in dogs or cats, and is poorly documented in veterinary medicine. The aim of this study was to determine the B. miyamotoi presence in (i) host-seeking ticks and (ii) engorged Ixodes sp. ticks collected from dogs and ca…

Analysis of the distribution of the tick Ixodes ricinus L. (Acari: Ixodidae) in a nature reserve of western Germany using Geographic Information Systems.

From May to November 2003, at five selected sites of the 'Siebengebirge', a nature reserve near Bonn, Germany, data on microclimate, pedology, plant sociology and tick abundance were collected weekly and correlated. A total of 2832 host-seeking Ixodes ricinus (2660 nymphs and 172 adults) were collected, with maximal abundance in June and a minor second increase in abundance in August. The spatial and temporal variation in tick abundance was closely related to the air temperature, relative humidity, soil water content and specific vegetation type. Increases in tick abundances in individual habi…

Transstadial and transovarial persistence of Babesia divergens DNA in Ixodes ricinus ticks fed on infected blood in a new skin-feeding technique.

Although Babesia divergens is the the principal confirmed zoonotic Babesia sp. in Europe, there are gaps in our knowledge of its biology and transmission by the tick Ixodes ricinus. In order to reproduce the part of the parasite cycle that occurs in the vector, an in vitro animal skin feeding technique on blood containing in vitro cultivated B. divergens was developed. Parasite DNA was detected in all samples of salivary glands of nymphs and adults that had fed on parasitized blood as larvae and nymphs, respectively, indicating acquisition as well as a transtadial persistence of B. divergens. …

Распространяется среди членов группы Borrelia burborferi sensu lato и Anaplasmaciae в клещах Ixodes ricinus в Эльзасе, в центре эндемичности Lime borrelios во Франции.

В связи с высокой распространенностью борелиоза в Эльзасе, на северо-востоке Франции, мы провели в 2003-2004 годах расследование по трем кантонам этого региона, с тем чтобы определить плотность клещей иксодов, инфицированных Боррелией бурдорфери sensu lato и Anaplasmaciae. Самая высокая плотность немфов, инфицированных B. burborferi sensu lato в Манстере и Гебвиллере, где заболеваемость была высокой, была одной из самых высоких в Европе (105 и 114 на 100 м(2), соответственно). С другой стороны, максимальная плотность инфицированных имфлей была низкой в кантоне Даннемари (5/100 м(2)), где забол

Совпадение в аннотации оригинала
Due to the high Lyme borreliosis incidence in Alsace, in northeastern France, we investigated in 2003-2004 three cantons in this region in order to determine the density of Ixodes ricinus ticks infected by Borrelia burgdorferi sensu lato and Anaplasmataceae. The peak density of nymphs infected by B. burgdorferi sensu lato at Munster and Guebwiller, where the…

Evidence for high activity of xylem parenchyma and ray cells in the interface of host stem and Agrobacterium tumefaciens-induced tumours of Ricinus communis.

Rapidly developing tumours at hypocotyls of Ricinus communis, induced by Agrobacterium tumefaciens strain C58, were characterized by strong differentiation of vascular bundles and their functional connection to the host bundles. The stem/tumour interface showed increased xylem, with numerous vessels accompanied by multiseriate unlignified rays. To know how nutrients efficiently accumulate in the tumour sink tissue, cell electropotentials (E(m)) in cross-sections were mapped. The measured cells were identified by injected Lucifer Yellow. Xylem and phloem parenchyma cells and stem/tumour-located…

Ультраструктурная локализация гемолимфа-лектина в гемоцитах и других тканях твердого клеща Иксодеса (Acari; Chelicerata).

Было предложено, чтобы Лектины функционировали как молекулы распознавания образов в беспозвоночных иммунных механизмах. Был описан лектин из … том, что гемолимифный лектин может функционировать как молекула распознавания в иммунной системе I. ricinus. Еще одной функцией может быть защита яиц, покрытых секрециями органов Гена. Лектиновая активность также может быть связана с передачей борелия бурдорфери, причинно-следственного агента боррелиоза Лайма, и вируса энцефалита, переносимого клещей.

The Occurrence of Borrelia burgdorferi sensu lato in Ixodes ricinus Ticks Collected from Nature-Educational and Tourist Trails in the Poprad Landscape Park.

Throughout Europe, including Poland, Ixodes ricinus ticks are the main vector of numerous pathogenic agents that pose a serious threat to public health. Southern Poland attracts many tourists with its scenic landscapes and abundant recreational opportunities. These areas are ideal habitats for wild fauna, which serve as the main reservoirs and hosts for these pathogens and ticks. The large population and biodiversity of these hosts facilitate the proliferation of ticks. The aim of this study was to determine the potential exposure of humans to ticks and tick-borne pathogens such as Borrelia bu…

Distribution of Ixodes ricinus ticks and prevalence of tick-borne encephalitis virus among questing ticks in the Arctic Circle region of northern Norway.

This study investigated the geographical distribution of Ixodes ricinus and prevalence of the tick-borne encephalitis virus (TBEV) in northern Norway. Flagging for questing I. ricinus ticks was performed in areas ranging from Vikna in Nord-Trøndelag County, located 190km south of the Arctic Circle (66.3°N), to Steigen in Nordland County, located 155km north of the Arctic Circle. We found that ticks were abundant in both Vikna (64.5°N) and Brønnøy (65.1°N). Only a few ticks were found at locations ∼66°N, and no ticks were found at several locations up to 67.5°N. Real-time PCR (RT-PCR) analyses …

Detection of Rickettsia helvetica in Ixodes ricinus infesting wild and domestic animals and in a botfly larva (Cephenemyia stimulator) infesting roe deer in Germany.

Ixodes ricinus is a well-known vector of different human pathogens including Rickettsia helvetica. The role of wild mammals in the distribution and probable maintenance of Rickettsia in nature is still to be determined. We therefore investigated various parasites from different wild mammals as well as companion animals for the presence of Rickettsia. A total of 606 I. ricinus, 38 Cephenemyia stimulator (botfly larvae), one Dermacentor reticulatus, 24 Haematopinus suis (hog lice) and 30 Lipoptena cervi (deer flies) were collected from free-ranging animals during seasonal hunting, and from compa…

Long-term study of the prevalence of Borrelia burgdorferi s.l. infection in ticks (Ixodes ricinus) feeding on blackbirds (Turdus merula) in NE Poland.

Seeking evidence to confirm that blackbirds (Turdus merula) may be involved in environmental maintenance of Borrelia burgdorferi s.l. (the causative agent of Lyme borreliosis), we conducted a long-term study over three separate 2-year periods, together embracing a span of almost 20 years, all in the same area in northeastern Poland. We examined a total of 78 blackbirds and collected 623 Ixodes ricinus ticks feeding on them. The tick infestation prevalence was found to be very high (89.7 %). Among all ticks collected, 9.8 % individuals were infected with B. burgdorferi s.l. spirochetes. We foun…

Which forest bird species are the main hosts of the tick, Ixodes ricinus, the vector of Borrelia burgdorferi sensu lato, during the breeding season?

Wild birds are important hosts for vector-borne pathogens, especially those borne by ticks. However, few studies have been conducted on the role of different bird species within a community as hosts of vector-borne pathogens. This study addressed individual and species factors that could explain the burden of Ixodes ricinus on forest birds during the reproductive periods of both vectors and hosts. The goal was to identify which bird species contribute the most to the tick population at the community level. Birds were mist-netted on four plots in 2008 and on seven plots in 2009 in two forests (…

Density of questing Ixodes ricinus nymphs and adults infected by Borrelia burgdorferi sensu lato in Switzerland: spatio-temporal pattern at a regional scale.

Lyme borreliosis, the most important vector-borne disease in the Northern hemisphere, causes health problem for populations in endemic areas. In the present study, the density of questing Ixodes ricinus ticks and their infection with Borrelia burgdorferi sensu lato (sl) was examined in 11 areas located on the Swiss Plateau and in an alpine valley. From 1999 to 2001, free-living I. ricinus ticks were collected on a monthly basis by flagging vegetation in these areas. Each tick was examined for the presence of B. burgdorferi sl using direct fluorescent antibody assay, and for isolation of the ba…

Tick-borne encephalitis virus prevalence in Ixodes ricinus ticks collected in high risk habitats of the south-Bohemian region of the Czech Republic.

A survey for tick-borne encephalitis virus in trades ricinus ticks was conducted in May 2000 in two districts of the South-Bohemian region of the Czech Republic with a high occurrence of tick-borne encephalitis (TBE) in humans. Homogenized ticks were tested on PS cells, which were examined for any cytopathic effect, plaque assay and indirect fluorescent antibody test (IFT). The IFT has proved to be the most sensitive and most rapid test to demonstrate the virus in ticks. TBE virus was found in 17 of 187 pooled samples. consisting of a total of 2,968 ticks. The mean minimum infection rate was 0…

Change in phenotype of tick-borne encephalitis virus following passage in Ixodes ricinus ticks and associated amino acid substitution in the envelope protein.

Serial passage of an uncloned tick-borne encephalitis virus (strain 4387 isolated from the liver and lungs of a bank vole) in Ixodes ricinus ticks, was accompanied by gradual reduction in virulence of the virus, as indicated by transmission of virus by infected ticks feeding on laboratory mice. After the 7th serial passage in ticks (strain 4387/7), 95% of mice survived the bite of infected ticks. The surviving infected mice showed either no or only low viraemia although virus could be isolated from the brains of some mice 14 and 30 days after commencement of tick feeding, implying that the tic…

Meta-transcriptomic profiling of Ixodes ricinus and Ixodes persulcatus ticks from Finland reveals diverse RNA viromes, community structuring, and emerging viral lineages.

Tick-borne viruses (TBVs) threaten human and animal health in northern Europe, where Ixodes ricinus and Ixodes persulcatus are expanding their range. In Finland, the genetic diversity, population structure, and tick-associated RNA virome of these species remain insufficiently characterized. Characterizing tick-borne viruses in Finland is essential to detect emerging pathogens at the northern edge of tick expansion, where climate-driven ecological change may facilitate the spread of novel viruses with potential risks to human and animal health. We applied single-tick metatranscriptomic approach…