Результаты поиска

[Effect of moxibustion-induced deqi on hippocampal autophagy function in APPswe/PSEN1dE9 transgenic Alzheimer's disease mice].

OBJECTIVE: To investigate the effect of moxibustion on autophagy regulation under deqi (arrival of qi ) versus non-deqi moxibustion, using APPswe/PSEN1dE9 transgenic Alzheimer's disease (AD) model mice. METHODS: Forty SPF-grade male APPswe/PSEN1dE9 transgenic mice were randomly divided into a model group (n=10) and a moxibustion group (n=30) at a ratio of 1∶3. Ten SPF-grade male C57BL/6 mice served as the control group. In the moxibustion group,gentle moxibustion was operated at the site around 3 cm far from "Dazhui" (GV14), 40 min each intervention, once daily and for 28 consecutive days. Th…

Folic acid protects against Toxoplasma gondii antigen-induced vascular injury via the CD36-autophagy-lysosomal axis.

Whether and how pathogen-derived antigens themselves drive vascular injury remains an unresolved question in infection biology and cardiovas … ike phenotype. This shift enhances CD36‑dependent antigen uptake and subsequently perturbs the macroautophagy/autophagy-lysosomal pathway (ALP), culminating in cellular homeostasis disruption. Interestingly, prophylactic folic acid (FA) ameliorates T. gondii STAg-induced vascular injury. FA acts by suppressing CD36 expression, thereby limiting STAg uptake a …

LINC01186-mediated deubiquitination of ACSL4 reprograms lipid metabolism to increase autophagy-mediated angiogenesis in colorectal cancer.

Angiogenesis supports colorectal cancer (CRC) proliferation and metastasis, yet how lipid metabolic reprogramming intersects with angiogenes … , eicosapentaenoic acid, and docosahexaenoic acid. Notably, elevated PUFA levels protect VEGFA from autophagy-lysosomal degradation by reducing autophagic flux, resulting in VEGFA overexpression and consequent promotion of angiogenesis. The absence of LINC01186 or ACSL4 impairs this signaling cascade, reduces PUFA levels, reactivates the autophagic degradat …

GPX3 activates autophagy to protect cochlear spiral ganglion neurons from injury in age-related hearing loss.

Age-related hearing loss (ARHL) represents the most common sensory disorder among the elderly population. Degeneration of spiral ganglion ne … ificantly decreased, accompanied by increased oxidative stress and apoptosis with aging. Meanwhile, autophagy levels were downregulated in SGNs with increased age. Targeted upregulation of GPX3 in SGNs markedly reduced the hearing threshold, improved wave I amplitude, and alleviated oxidative stress and apoptosis in SGNs. Interestingly, overexpression of GP …

Отличительные механизмы аутопсии при стрессовом воздействии с помощью вирусных штаммов с высоким и низким коэффициентом псевдообеспечения.

Вирус псевдорабии (ППП) является одним из наиболее значительных патогенов, угрожающих свиньи, и его инфекция повлекла за собой значительные экономические потери для свиноводства во всем мире. В этом исследовании использовались два штамма PRV, изолированных нашей лабораторией: штамм GXLB-2015, натуральный рекомбинант между штаммом PRV-типа и штаммом Bartha-K61 вакцины, который демонстрирует более сильную вирулентность, и штамм GXGG-2016, классический генотип II, штамм PRV с естественным удалением 69-амино кислоты в гене ТЗ, который демонстрирует слабую вирулентность и непатогенен в мышах. Сосре

Совпадение в аннотации оригинала
Pseudorabies virus (PRV) is one of the most significant pathogens threatening the swine industry, and its infection has caused substantial e … irulence and is non-pathogenic in mice. Focusing on endoplasmic reticulum stress (ERS) and cellular autophagy, this research explored the molecular mechanisms underlying the significant difference in virulence between these two PRV strains. The results revealed that infection with both strains induced noticeable ERS and autophagy, both of which inhibited vi …

Targeting autophagy in oral squamous cell carcinoma chemoresistance: molecular mechanisms, therapeutic strategies, and emerging nanotherapeutic approaches.

Autophagy is a lysosome-dependent recycling process that maintains cellular homeostasis and helps cells adapt to therapeutic stress. In oral squamous cell carcinoma (OSCC), dysregulated autophagy may promote chemoresistance by supporting metabolic adaptation, removing damaged cellular components, and limiting treatment-induced cell death. Its effects are nevertheless context dependent, as autophagy can also interact with apoptosis, ferroptosis, and other cytotoxic pathways. This review summarizes molecular mechanisms linking autophagy to OSCC chemoresistance, focusing on non-coding RNAs, p53/T…

miR-486-5p suppresses autophagy in hepatocellular carcinoma via activation of the AKT/mTOR signalling pathway.

Autophagy is controlled by a complex signalling network and is closely linked to the initiation and progression of hepatocellular carcinoma (HCC). However, how microRNAs (miRNAs) regulate autophagy in HCC remains incompletely understood. In this study, we identified microRNA-486-5p (miR-486-5p) as a tumour-associated microRNA that is enriched in circulating exosomes derived from patients with HCC. Elevated miR-486-5p promoted proliferation and suppressed apoptosis in HCC cell lines under stress conditions. Mechanistically, miR-486-5p directly targets the 3' untranslated region of the phosphata…

Inhibition of NHE1 Overcomes Temozolomide-Resistance in Glioblastoma via ROS-AKT/ERK Axis-Mediated Autophagy Suppression.

Glioblastoma (GBM) is the most aggressive primary brain tumor in adults, with temozolomide (TMZ) resistance being a major barrier to improved clinical outcomes. Dysregulated autophagy is closely associated with TMZ resistance in GBM, but the role of sodium/hydrogen exchanger 1 (NHE1)-a key regulator of intracellular pH homeostasis-in this process remains uncharacterized. We established a TMZ-resistant GBM cell line (U251TR) and observed that U251TR cells displayed higher autophagic activity, increased reactive oxygen species (ROS) levels, and reduced apoptotic rates compared to parental U251 c…

Umbilical cord mesenchymal stem cell-derived extracellular vesicles improve excessive autophagy of granulosa cells through METTL3.

Polycystic ovary syndrome (PCOS) is a prevalent endocrine disorder impacting women's fertility. We assessed the effect of umbilical cord mesenchymal stem cell-derived extracellular vesicles (UC-MSC-EVs) on PTEN-induced kinase 1 (PINK1)/Parkin-mediated excessive autophagy of ovarian granulosa cells (GCs) through methyltransferase-like 3 (METTL3). Human ovarian GC line KGN was cultured and treated with dehydroepiandrosterone (DHEA) and UC-MSC-EVs. Cell apoptosis and viability, autophagy-related protein levels, adenosine triphosphate (ATP) and mitochondrial membrane potential (MMP) level, and mic…

FTO-Mediated m6A Demethylation of CCL2 Promotes Gastric Cancer Progression via Autophagy and Oxidative Stress Regulation.

BACKGROUND: Gastric cancer (GC) remains a leading cause of cancer-related mortality worldwide due to late-stage diagnosis and therapeutic resistance. Oxidative stress and autophagy play paradoxical roles in GC progression, yet their regulatory mechanisms remain incompletely understood. This study investigates the molecular interplay between chemokine CCL2, oxidative stress, autophagy, and m6A RNA demethylase fat mass and Obesity-associated protein (FTO) in GC pathogenesis. METHODS: Oxidative stress-related genes were identified through bioinformatics analysis using the GeneCards and GEO datab…

Hepatitis C Virus NS5A Inhibits YAP1-HSC70 Interaction, Thereby Preventing YAP1 Degradation Via Chaperone-Mediated Autophagy.

Hepatitis C virus (HCV) infection induces chaperone-mediated autophagy (CMA), where the HCV NS5A protein promotes the binding of the molecular chaperone HSC70 to substrate proteins containing a KFERQ motif. HSC70 recognizes this pentapeptide motif and transports substrates to lysosomes for degradation. In this study, we identified a KFERQ motif (324ELLRQ328) in the YAP1 protein, a key regulator of the Hippo pathway, and examined its interaction with HSC70 during HCV infection. To determine whether HSC70 directly binds to this motif, we generated four YAP1 mutants with specific alterations in t…

Formosanin C Induces ROS/p38-Mediated Cell Death While Also Promoting Protective Autophagy in Pancreatic Cancer.

PDAC, also known as pancreatic ductal adenocarcinoma, is a highly aggressive form of cancer that currently lacks efficient treatment options … as also accountable for cell death initiated by FC. Interestingly, FC also induced a cytoprotective autophagy in PDAC cells; blocking autophagy further enhanced the inhibited cell proliferation. Experiments conducted in living organisms demonstrated that FC inhibited the proliferation of PDAC tumors in mice. FC suppresses PDAC tumor growth through ROS/p38-m …

Hypoxia inhibits intervertebral disc degeneration by maintaining autophagy circadian rhythm via the HIF1α-PER2-mTOR pathway.

INTRODUCTION: Disruption of the circadian rhythm (CR) and autophagy in intervertebral discs contributes to intervertebral disc degeneration (IDD) progression. However, the circadian regulation of autophagy requires further investigation. OBJECTIVES: We observed the expression of circadian proteins and autophagic markers of nucleus pulposus (NP) cells followed a diurnal rhythmic pattern in vivo and in vitro. METHODS: NP tissues were collected from light/dark cycle-shifted rats and IDD patients of varying severity. CR and ECM-related proteins were analyzed by immunohistochemistry and western b…

Grandifloridin D: A Potent Antiausterity Agent Targeting Pancreatic Cancer Cells via Akt/mTOR and Autophagy Inhibition.

The hypovascular nature of pancreatic tumors creates a nutrient-scarce, hypoxic microenvironment, yet pancreatic cancer cells adapt by alter … inase B (Akt) and mammalian target of rapamycin (mTOR) signaling pathway while also suppressing the autophagy-related proteins microtubule-associated protein 1 light chain 3 (LC3). These results suggest that grandifloridin D is a promising antiausterity agent for pancreatic cancer drug development.

Sphingosine kinase 2 deficiency impairs VLDL secretion by inhibiting mTORC2 phosphorylation and activating chaperone-mediated autophagy.

Hepatic very low-density lipoprotein (VLDL) is essential for maintaining lipid metabolism in the liver. Sphingosine kinases (SphKs) are esse … y results in accelerated degradation of the SEC22B, STX5A, and GS28 proteins via chaperone-mediated autophagy (CMA), impeding VLDL transport to the Golgi apparatus. MYH1485, a specific activator of mTOR, induces mTORC2 phosphorylation, thereby inhibiting the degradation of SNARE complexes by CMA and counteracting the lipid accumulation induced by SphK2 defi …

Multi-omics study reveals differential expression and phosphorylation of autophagy-related proteins in autism spectrum disorder.

Our multi-omics study investigated the molecular mechanisms underlying autism spectrum disorder (ASD) using Shank3Δ4-22 and Cntnap2-/- mouse models. Through global- and phospho- proteomics of the mouse cortex, we focused on shared molecular changes and found that autophagy was particularly affected in both models. Global proteomics identified a small number of differentially expressed proteins that significantly impact postsynaptic components and synaptic function, including key pathways such as mTOR signaling. Phosphoproteomics revealed unique phosphorylation sites in autophagy-related protei…

Unveiling the nexus between environmental exposures and testicular damages: revelations from autophagy and oxidative stress imbalance.

Recent evidence consolidates the deleterious impact of environmental exposure on testicular damage. Environmental exposures can instigate testicular toxicity, causing damage to the Sertoli-Sertoli cell-mediated blood-testis barrier (BTB) integrity, alterations in hormone levels orchestrated by aberrant Leydig cells, and disruption of spermatogenesis. Despite diverse study designs and methodologies, a consensus is emerging on how environmental factors induce oxidative stress by elevating ROS levels, affecting autophagy through pathways such as the ROS-mediated mTOR signaling pathway, ultimately…

Targeting LKB1-AMPK-SIRT1-induced autophagy and mitophagy pathways improves cerebrovascular homeostasis in APP/PS1 mice.

BACKGROUND: Alzheimer's disease (AD) is the most common and severe degenerative disorder of the central nervous system in the elderly, profo … irectly bind to AMPK and LKB1 proteins, activating the LKB1-AMPK-SIRT1 signaling pathway, promoting autophagy and mitochondrial autophagy, and alleviating oxidative stress damage in endothelial cells. CONCLUSIONS: BZBS enhances autophagy-related activity, decreases Aβ deposition, and improves endothelial cell homeostasis through the activation of the LKB1- …

Berberine alleviates Alzheimer's disease by activating autophagy and inhibiting ferroptosis through the JNK-p38MAPK signaling pathway.

INTRODUCTION: Alzheimer's disease (AD) is a neurodegenerative disease characterized by amyloid beta (Aβ) deposition, phosphorylated Tau protein aggregation, inflammation, and neuronal damage. Neuronal autophagy plays an important role in ameliorating central nervous system diseases such as AD. As an emerging form of iron-dependent cell death, ferroptosis has attracted great attention in the field of neurodegenerative diseases. Berberine (BBR), a natural alkaloid, has demonstrated excellent in inflammation reduction, inhibition of Aβ production, and neuroprotection, making it a potential candid…

MicroRNA-361-3p Regulates Autophagy and Apoptotic Processes by Regulating PI3K/Akt Signaling in Parkinson's Disease.

MicroRNAs are closely related to the pathogenesis of Parkinson's disease (PD). The purpose of this study was to explore the effects of microRNA (miR)-361-3p on apoptosis and autophagy in 1-methyl-4-phenylpyridinium ion (MPP+) induced PD cell models, and its potential mechanisms. SH-SY5Y cells were induced by 1.0 mM MPP+ for 24 h to establish a PD cell model in vitro, and the expression level of miR-361-3p was regulated by cell transfection. Cell viability was detected by the Cell Count Kit (CCK)-8 method. Apoptosis was evaluated by flow cytometry. Inflammatory factors were detected by enzyme-l…