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Beyond the Classroom: The School-To-Prison Pipeline as a Public Health Crisis.

The School-to-Prison Pipeline (STPPL) is primarily positioned in the discourse relating to education and criminal justice yet its public health implications remain underexplored. This commentary draws the connections between STPPL and social determinants of health on Macro, Mezzo, and Micro levels. The STPPL disrupts access to mental health care, nutritional programs, and safety systems embedded within schools. Exclusionary practices such as suspensions and expulsions disconnect students from critical services that promote psychological stability and physical well-being Obsuth et al. (2024). N…

Prussian blue modified carbon black nanoparticles enabling record sensitivity of hydrogen peroxide electrochemical sensors.

We report on the deposition of Prussian blue nanoparticles onto the carbon black supports in course of reduction of FeCl3/K3Fe(CN)6 equimolar mixture by hydrogen peroxide. This one-pot synthesis eliminates the use of volatile organic solvents as well as additional synthetic or mixing steps. With an increase of carbon black loading, the hydrodynamic size of the resulting nanocomposites displays the well-defined maximum and reaches the plateau at 115 ± 10 nm. Modified electrodes were fabricated by simple drop-casting of the nanoparticle suspensions onto the surface of screen-printed carbon struc…

Clinical applications of tacrolimus in the treatment of ocular diseases: a narrative literature review.

PURPOSE: To narratively review clinical use of topical and periocular tacrolimus in ophthalmology, with emphasis on allergic keratoconjunctivitis (VKC/AKC), ocular GVHD, post‑keratoplasty prophylaxis, and selected keratitis/uveitis indications. METHODS: Narrative search of PubMed/Scopus through October 2025 focusing on prospective, randomized, and comparative studies of topical/periocular tacrolimus; recent formulation advances (nanoemulsions, in‑situ gels, cyclodextrin complexes) were included. RESULTS: Across VKC/AKC, tacrolimus (0.03-0.1% ointment; 0.05-0.1% solutions/suspensions) improve…

Системы реагирования на полимеразы в режиме реального времени для обнаружения и дифференцирования неклассифицированных вирусов семейства фенуивирида.

Семья Фенавирида, являющаяся частью порядка Hareaviriales, включает в себя арбовирусы и вирусы, связанные с артроподом, с песчаниками, комарами и клещами в качестве основных векторов. Исторически только фенуивирузы, передающиеся через песчаные/москито, были связаны с человеческими заболеваниями, однако появление сильной лихорадки с синдромом тромбоцитопенсии (СФТС) выявило потенциальные возможности фенуивирузы, переносимые тиками, в качестве патогенов человека. Недавние открытия новых вирусов, связанных с артроподом, некоторые из которых остаются неклассифицированными, подчеркивают необходимос

Совпадение в аннотации оригинала
The family Phenuiviridae, part of the order Hareavirales, includes arboviruses and arthropod-associated viruses, with sandflies, mosquitoes, … fying and differentiating unclassified viruses within the Phenuiviridae family. In this study, tick suspensions containing phenuiviruses, previously obtained during the screening of ticks from various regions of Russia using pan-phenuivirus primers, were used. Specific primers and probes were designed to differentiate five Phenuiviridae viruses of genera Uuku …

Establishment and characterization of cMYB-expressing human salivary adenoid cystic carcinoma cell lines (UM-HACC-14, UM-HACC-6) and matching patient-derived xenograft model (UM-PDX-HACC-14).

OBJECTIVE: Limited availability of authentic human adenoid cystic carcinoma (ACC) cell lines has hindered progress in understanding mechanisms underpinning the biology of this disease and the development of safe and effective therapies. STUDY DESIGN: Surgical human ACC specimens (UM-HACC-6, UM-HACC-14) were dissociated into single cell suspensions and cultured in fibronectin-coated flasks. Alternatively, tumor fragments were transplanted subcutaneously into female immunodeficient (SCID) mice to establish patient-derived xenograft tumors (PDX; UM-PDX-HACC-14). RESULTS: Both ACC cell lines sho…

The effect of ionic redistributions on the microwave dielectric response of cytosol water upon glucose uptake.

The sensitivity of cytosol water's microwave dielectric (MD) response to D-glucose uptake in Red Blood Cells (RBCs) allows the detailed study of cellular mechanisms as a function of controlled exposures to glucose and other related analytes like electrolytes. However, the underlying mechanism behind the sensitivity to glucose exposure remains a topic of debate. In this research, we utilize MDS within the frequency range of 0.5-40 GHz to explore how ionic redistributions within the cell impact the microwave dielectric characteristics associated with D-glucose uptake in RBC suspensions. Specific…

Isolation of single cells from human uterus in the third trimester of pregnancy: myometrium, decidua, amnion and chorion.

During pregnancy, interactions between uterine immune cells and cells of the surrounding reproductive tissues are thought to be vital for re … nt. The protocols can be completed in tandem and take approximately 4-5 h, resulting in single-cell suspensions that contain viable leucocytes, and non-immune cells in sufficient numbers for single-cell analysis approaches such as flow cytometry and single cell RNA sequencing (scRNAseq).

Scalable production and complete biophysical characterization of poly(ethylene glycol) surface conjugated liposome encapsulated hemoglobin (PEG-LEH).

Particle encapsulated hemoglobin (Hb)-based oxygen (O2) carriers (HBOCs) have clear advantages over their acellular counterparts because of … l suitability and efficacy as an RBC replacement. Our results demonstrate that PEG-LEH nanoparticle suspensions manufactured via a high-pressure cell disruptor have Hb concentrations comparable to whole blood (~12 g/dL) and possess other desirable characteristics, which may permit their use as potential lifesaving O2 therapeutics.

In Vitro Transformation and Selection of Treponema pallidum subsp. pallidum.

Although the isolation of Treponema pallidum subsp. pallidum (T. pallidum) from a syphilis patient dates to 1912, for the duration of the 20 … asic Protocol 1: Transformation of T. pallidum Support Protocol 1: Quantification of T. pallidum in suspensions using dark-field microscopy Support Protocol 2: Counting cells using a hemacytometer Basic Protocol 2: Selection, initial passaging, and expansion of transformed cultures Basic Protocol 3: Isolation of a clonal strain through limiting dilution.

Effects of Pediococcus pentosaceus LI05 on immunity and metabolism in germ-free rats.

Many Pediococcus spp. have health-promoting benefits, and Pediococcus pentosaceus LI05 is one such species that was proved to be beneficial in previous studies. Our research aimed to determine the immune and metabolic effects of P. pentosaceus LI05 on germ-free rats. Germ-free rats were gavaged with P. pentosaceus LI05 suspensions (1 × 109 CFU) for 2 weeks, and 3 weeks later, blood, spleen, intestine and liver samples were gathered for metabolome, intestine morphology, immunity, and transcriptomics analyses. Oral gavage of P. pentosaceus LI05 reduced the bodyweight of rats, which manifested as…

Characterization 0.1 wt.% Nanomaterial/Photopolymer Composites with Poor Nanomaterial Dispersion: Viscosity, Cure Depth and Dielectric Properties.

Notably, 3D printing techniques such as digital light processing (DLP) have the potential for the cost-effective and flexible production of … propyl alcohol, and ethanol) before adding photopolymer and continuing dispersion. For all prepared suspensions, a reduction in viscosity is observed, which is favorable for 3D printing. In contrast, the addition of 0.1 wt.% nanomaterials, even with poor dispersion, leads to curing depth reduction up to 90% compared to pristine photopolymer, where the nanomat …

Bone Marrow Immunohistochemistry and Flow Cytometry in the Diagnosis of Malignant Hematologic Diseases With Emphasis on Lymphomas: A Comparative Retrospective Study.

We aim to evaluate the degree of agreement between immunohistochemistry (IHC) and flow cytometry (FC) in the diagnosis of malignant hematologic diseases, mainly lymphomas. A total of 260 bone marrow biopsies, 255 bone marrow aspirates, and 5 other suspensions of 260 patients used for diagnosis of a hematologic malignancy between 2009 and 2012 with both, IHC and FC, were retrospectively analyzed. Overall there is a substantial degree of agreement (κ=0.69) between IHC and FC. Chronic lymphocytic leukemia/small lymphocytic lymphoma, mature T-cell neoplasms, acute leukemias, and myelodysplastic sy…

Characterization of Chicken Leukocyte Subsets from Lymphatic Tissue by Flow Cytometry.

Determining the abundance of leukocyte subtypes, including lymphocyte subpopulations, not only in blood but also in lymphatic tissues, is in … cells among intraepithelial lymphocytes in chicken cecal tonsils. For this, we prepared single-cell suspensions of spleen and isolated intraepithelial lymphocytes from cecal tonsils without density centrifugation, and performed antibody staining of cells without subsequent washing steps to prevent cell loss and falsification of obtained cell counts. © 2020 Th …

Anti-pathogenic activity of graphene nanomaterials: A review.

Graphene and its derivatives are promising candidates for a variety of biological applications, among which, their anti-pathogenic propertie … ity of graphene flakes, as well as the concentration, contact time and the pH value of the graphene suspensions used in related microbial tests. We discuss the possible surface and edge interactions between bacterial cells and graphene nanomaterials, which cause antibacterial effects such as membrane/oxidative/photothermal stresses, charge transfer, entrapmen …

Synthesis and Characterization of Long-Acting Darunavir Prodrugs.

Antiretroviral therapy (ART) has improved the quality of life in patients infected with HIV-1. However, complete viral suppression within an … 1DRV and M2DRV, were synthesized as lipophilic and hydrophobic prodrugs and stabilized into aqueous suspensions designated NM1DRV and NM2DRV. The formulations demonstrated enhanced intracellular prodrug levels with sustained drug retention and antiretroviral activities for 15 and 30 days compared to native DRV formulation in human monocyte-derived macrophages …

Cross-reactivity in serological tests for brucellosis: a comparison of immune response of Escherichia coli O157:H7 and Yersinia enterocolitica O:9 vs Brucella spp.

According to European Union (EU) regulations, the serological tests for the eradication of bovine and ovine brucellosis are the Rose Bengal Test, Complement Fixation Test, and i-ELISA. These methods, also recommended by the World Organization for Animal Health (OIE) for international trades, have limitations related to the use of suspensions of smooth Brucellae or LPS extracts. Limitations include false-positive serological reactions to brucellosis, which in turn impedes accurate diagnosis in some herds. False positive reactions should be considered carefully during the final stages of an erad…

Development of local strontium ranelate delivery systems and long term in vitro drug release studies in osteogenic medium.

It has been recognized that the operative stabilization of osteoporotic fractures should be followed up with an appropriate osteoporosis tre … d within 121 days. In vitro cell viability evaluation approved no cytotoxic effects of microcapsule suspensions and extracts.

Recombinant anthrax protective antigen: Observation of aggregation phenomena by TEM reveals specific effects of sterols.

Negatively stained transmission electron microscope images are presented that depict the aggregation of recombinant anthrax protective antigen (rPA83 monomer and the PA63 prepore oligomer) under varying in vitro biochemical conditions. Heat treatment (50°C) of rPA83 produced clumped fibrils, but following heating the PA63 prepore formed disordered aggregates. Freeze-thaw treatment of the PA63 prepore generated linear flexuous aggregates of the heptameric oligomers. Aqueous suspensions of cholesterol microcrystals were shown to bind small rPA83 aggregates at the edges of the planar bilayers. Wi…

Varying the counter ion changes the kinetics, but not the final structure of colloidal gels.

We show that, while the gelation of colloidal silica proceeds much faster in the presence of added KCl than NaCl, the final gels are very similar in structure and properties. We have studied the gelation process by visual inspection and by small angle X-ray scattering for a range of salt and silica particle concentrations. The characteristic times of the early aggregation process and the formation of a stress-bearing structure with both salts are shown to collapse onto master curves with single multiplicative constants, linked to the stability ratio of the colloidal suspensions. The influence …

Developing functionalized Fe3O4-Au nanoparticles: a physico-chemical insight.

Nanotechnology for biomedicine has recently attracted increasing interest from the scientific community. In particular, among the different … be here an optimized and efficient functionalization protocol to obtain stable nanoparticle aqueous suspensions, which can be easily exported to other kinds of metal and metal-oxide NPs and used to develop biocompatible systems. Furthermore, reliable information that could be useful for researchers working in this field is extensively discussed.