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Anti-inflammatory control of human skin keratinocytes by targeting nuclear transport checkpoint.

BACKGROUND: In the two common inflammatory skin diseases, Atopic Dermatitis (AD) and Psoriasis (Ps), keratinocytes (KCs) respond to immune insults through activation of proinflammatory transcription factors (TFs) and their translocation to the cell's nucleus. Therein, the TFs induce expression of genes encoding mediators of skin inflammation. The Nuclear Transport Checkpoint Inhibitors (NTCIs) were developed to regulate nuclear translocation of activated TFs, the essential step of inflammatory response. This new class of cell-penetrating peptide therapeutics controls inflammation caused by all

A Murine Model of Vesicant-Induced Acute Lung Injury.

Burn injuries including those caused by chemicals can result in systemic effects and acute lung injury (ALI). Cutaneous exposure to Lewisite, a warfare and chemical burn agent, also causes ALI. To overcome the limitations in conducting direct research on Lewisite-induced ALI in a laboratory setting, an animal model was developed using phenylarsine oxide (PAO) as a surrogate for Lewisite. Due to lack of a reliable animal model mimicking the effects of such exposures, development of effective therapies to treat such injuries is challenging. We demonstrated that a single cutaneous exposure to PAO

Genital HSV-1 DNA detection is associated with a low inflammatory profile in HIV-uninfected South African women.

OBJECTIVES: Genital herpes simplex virus (HSV) infections are common in South Africa and worldwide. While HSV-2 is known to cause genital lesions, HSV-1 is better known to cause oral infections. Due to the global rise in genital HSV-1 infections, we aimed to compare the genital cytokine environment associated with HSV-1 and HSV-2 infections and their relation to the proinflammatory genital immune environment associated with HIV risk in African women. METHODS: HSV-1 and HSV-2 DNA were detected by quantitative real-time PCR in menstrual cup specimens collected from 251 HIV-negative women partic

Plasma Inflammatory Biomarkers Associated with Advanced Liver Fibrosis in HIV-HCV-Coinfected Individuals.

BACKGROUND: HIV and HCV coinfection leads to accelerated liver fibrosis, in which microbial translocation and systemic inflammation might play important roles. OBJECTIVE: This study aimed to provide an extensive profile of the plasma microbial translocation and inflammation biomarkers associated with advanced liver fibrosis among HIV-HCV-coinfected patients. METHODS: This cross-sectional study recruited 343 HIV-HCV-coinfected patients on combination antiretroviral therapy (cART) from a rural prefecture of Yunnan province in Southwest China. The plasma concentrations of sCD14 and 27 cytokines

Intracoronary Administration of Allogeneic Adipose Tissue-Derived Mesenchymal Stem Cells Improves Myocardial Perfusion But Not Left Ventricle Function, in a Translational Model of Acute Myocardial Infarction.

BACKGROUND: Autologous adipose tissue-derived mesenchymal stem cells (ATMSCs) therapy is a promising strategy to improve post-myocardial infarction outcomes. In a porcine model of acute myocardial infarction, we studied the long-term effects and the mechanisms involved in allogeneic ATMSCs administration on myocardial performance. METHODS AND RESULTS: Thirty-eight pigs underwent 50 minutes of coronary occlusion; the study was completed in 33 pigs. After reperfusion, allogeneic ATMSCs or culture medium (vehicle) were intracoronarily administered. Follow-ups were performed at short (2 days afte

Elevated levels of circulating interleukin-6 and transforming growth factor-beta1 in patients with metastatic prostatic carcinoma.

PURPOSE: Specific cytokines have been found to be secreted by and influence the growth of prostate cancers in cell culture. Interleukin-6 (IL-6), tumor necrosis factor alpha (TNFalpha), granulocyte macrophage-colony stimulating factor (GM-CSF) and transforming growth factor-beta1 (TGF-beta1) have all been closely associated with prostate cancer. We analyzed the levels of these cytokines in the systemic circulation of patients with varying stages of prostate cancer compared to controls. MATERIALS AND METHODS: Serum IL-6, TNFalpha and GM-CSF were measured using commercially available enzyme lin

Tumor-host interaction: analysis of cytokines, growth factors, and tumor-infiltrating lymphocytes in ovarian carcinomas.

The host-tumor interaction may play an important role in determining tumor progress. Recent studies have shown that this interaction can be influenced by the release of soluble factors by tumor cells and tumor-infiltrating lymphocytes (TIL). The aim of our study is to characterize the nature of cytokines and growth factors and their relationship to the cellular infiltrates in 16 patients with ovarian cancer using reverse transcriptase-polymerase chain reaction (RT-PCR) and immunohistochemistry. Total RNA from 20 malignant and 10 benign specimens were used to assay for expression of 12 cytokine

Antithyroid drug-induced agranulocytosis: clinical experience with ten patients treated at one institution and review of the literature.

The frequency, predisposing factors and course of agranulocytosis (granulocytes < 250/microliter) secondary to antithyroid drugs were studied in a cohort of 1256 continuously treated outpatients with hyperthyroidism during the 15 year period from 1973 to 1987. Two cases of agranulocytosis were detected; the frequency was 0.18% (95%-confidence intervals, 0.0-0.44%). This prevalence appears to be lower than reported in previous studies (up to 1.8%). For other adverse drug reactions, there was a clear-cut relationship to initial thionamide dose and to the body mass index; most reactions occurred

Expression of FLT3 receptor and response to FLT3 ligand by leukemic cells.

The novel hematopoietic growth factor FLT3 ligand (FL) is the cognate ligand for the FLT3, tyrosine kinase receptor (R), also referred to as FLK-2 and STK-1. The FLT3R belongs to a family of receptor tyrosine kinases involved in hematopoiesis that also includes KIT, the receptor for SCF (stem cell factor), and FMS. the receptor for M-CSF (macrophage colony- stimulating factor). Restricted FLT3R expression was seen on human and murine hematopoietic progenitor cells. In functional assays recombinant FL stimulated the proliferation and colony formation of human hematopoietic progenitor cells, i.e

Rescue Plerixafor-based Mobilization and Outcomes of Salvage High-dose Chemotherapy in Relapsed/Refractory Germ Cell Tumors: A Large Single-center Experience.

BACKGROUND: High-dose chemotherapy (HDCT) with autologous peripheral blood progenitor cell (PBPC) support represents a potentially curative … nd August 2023 who underwent PBPC mobilization with plerixafor in combination with chemotherapy and granulocyte colony-stimulating factor. The primary endpoint was successful CD34+ cell collection. Secondary endpoints included number of leukapheresis procedures, feasibility of HDCT, and survival outcomes. RESULTS: Twenty-two patients were included. All patients had poor-prognosis rel …

Outcomes of an Alpha-DC-1 Dendritic Cell-Based Vaccine Clinical Trial in Patients with Low-Tumor-Burden High-Risk Ovarian Carcinoma.

Background/Objectives: High-grade serous ovarian cancer (HGSOC) is usually discovered in advanced stages and often relapses shortly after initial conventional therapy. Survival in HGSOC patients might be improved with the use of novel immune therapies, which potentiate autologous anti-tumor responses. Dendritic cells (DCs) are potent antigen-presenting cells that can initiate immune responses, activate cytotoxic T cells and drive T-cell differentiation. This pilot trial evaluated the safety and efficacy of a unique DC vaccine (α-DC-1) in relapsed, advanced HGSOC patients with minimal tumor bur

Impact of age-related maculopathy susceptibility 2, high-temperature requirement A serine peptidase 1, and complement factor H genetic variants on clinical phenotypes of age-related macular degeneration.

PURPOSE: Age-related macular degeneration (AMD) is a leading cause of irreversible vision loss in the elderly. This study investigated the a … Cytokine profiling showed significantly reduced levels of erythropoietin (EPO) in AMD patients and granulocyte colony-stimulating factor (G-CSF) in controls (P = 0.044 and P = 0.023). CONCLUSION: This study establishes novel genotype-phenotype correlations for ARMS2, HTRA1, and CFH SNPs in a North Indian cohort, linking heterozygous/homozygous risk alleles to distinct AMD clinical f …

The VIVA Regimen (Vinorelbine, Ifosfamide, Vincristine, and Actinomycin-D) for High-Risk Rhabdomyosarcoma: Feasibility and Outcomes From a Single-Institution Study.

INTRODUCTION: Rhabdomyosarcoma is a highly chemosensitive tumor; however, high-risk and metastatic cases require more effective systemic treatments. This study evaluates the feasibility and efficacy of the "VIVA" regimen (vinorelbine-ifosfamide-vincristine-actinomycin D), which incorporates weekly vinorelbine into the induction phase of standard therapy. METHODS: Between 2020 and 2025, 45 patients (median age 14 years) with high-risk or metastatic rhabdomyosarcoma were treated at the Pediatric Unit of the Istituto Nazionale Tumori in Milan. The VIVA regimen added intravenous vinorelbine (25 m

Цитоплания и инфекция после цитакабагена автолейкеля в сильно обработанном реанимированном или рефрактном миеломе.

В феврале Управление по контролю за продуктами питания и лекарственными препаратами одобрило автолекцию Ciltaakabtagene (cilta-cel). 2022 для лечения повторной/реактивной множественной миэломы после four циклы терапии. По поводу CARTITUDE-1 в ходе судебного разбирательства, ≥3 Широко распространены цитопентия и инфекции. Здесь мы пытались охарактеризовать цитопению и инфекцию после вливания цита-села в стандартную систему ухода. Это многоцентральное, ретроспективное исследование включало 105 - пациенты, получившие цилта-сел; 91 Днем 90 и 49 Днем 180 о последующей деятельности. Класс должности

Совпадение в аннотации оригинала
Ciltacabtagene autoleucel (cilta-cel) was approved by the Food and Drug Administration in February 2022 for the treatment of relapsed/refrac … grade thrombocytopenia at apheresis was associated with grade ≥3 cytopenia at both days 30 and 90. Granulocyte colony-stimulating factor was administered to 65%, transfusion support to 38%, thrombopoietin agonists to 10%, intravenous immunoglobulins to 52%, and CD34+ stem cell boosts to 9.5% of patients. Infections occurred in 49% of patients and were severe in 32%. Earlier infection …

Safe Peripheral Stem Cell Collection in Pediatric Sibling Donors: A Single-Centre Experience From South India.

BACKGROUND: Peripheral blood stem cell (PBSC) collection from healthy pediatric sibling donors is increasingly preferred for allogeneic hema … collection at a single center in South India between June 2019 and April 2024. All donors received granulocyte colony-stimulating factor (G-CSF) 10 μg/kg/day for 5 days. Plerixafor (0.24 mg/kg) was administered on day 4 in donors with a donor-recipient weight ratio (DRWR) < 0.75. Apheresis was performed on day 5 using the Spectra Optia continuous mononuclear cell collection protocol. …

Chronic Neutropenia in Children: A Single-Center Experience.

OBJECTIVE: To analyze the characteristics, etiology, diagnostic evaluation, management, follow-up, and outcomes of children with chronic neutropenia treated at a tertiary care pediatric hospital in Croatia. MATERIALS AND METHODS: We retrospectively reviewed the demographic, clinical, and laboratory data of 48 children (23 males and 25 females; median age 7.75 months [IQR 5.13-11.75]) diagnosed with chronic neutropenia between 2013 and 2021. RESULTS: The median absolute neutrophil count (ANC) at presentation was 487/μL (IQR 198.5-837.5), and 52% of the patients had severe neutropenia. Autoimm

Normothermic Machine Perfusion Preserves Endothelial Clearance, Modulates Inflammation After Prolonged Warm Ischemia in Liver Grafts.

BACKGROUND: Prolonged warm ischemia time (WIT) increases susceptibility to ischemia-reperfusion injury in liver grafts. Viability assessment during normothermic machine perfusion (NMP) mainly targets hepatocyte and cholangiocyte function, whereas the contribution of liver sinusoidal endothelial cells (LSECs) is less explored. We evaluated whether clearance of hyaluronan (HA) and N-terminal propeptide of procollagen type III (PIIINP) reflects LSEC clearance function and predicts inflammatory activation during NMP. METHODS: In a porcine model with 45 min of WIT, livers were randomized to either

LA-GM-CSF, a Long-Acting Cytokine Mitigates and Prevents H-ARS Mediated Lethality in Mice Exposed to Total Body Gamma Radiation.

Widespread uses of nuclear materials increase the risk of accidental or intentional radiation exposure, which can result in acute radiation syndrome (ARS). Hematopoietic ARS (H-ARS) occurs at relatively low doses and is potentially lethal without intervention. While several FDA-approved cytokine-based radiomitigators exist, many require repeated dosing, complicating deployment in mass-casualty scenarios. This study evaluated a novel long-acting, murine-reactive granulocyte-macrophage colony-stimulating factor (LA-GM-CSF; mPDM608) as a prophylactic and mitigative countermeasure for H-ARS. Male

Peri-Transfer Glucocorticoid Therapy in Oocyte-Donation IVF Bridging the Immunological Gap.

In vitro fertilisation via oocyte donation is a unique reproductive technique in which the embryo is fully separate from the receiver. This … inutive and non-random, often integrating glucocorticoids with other therapies such as antibiotics, granulocyte colony-stimulating factor, or endometrial damage. These designs inhibit the dissociation of the independent impact of glucocorticoids. Recent comprehensive randomised studies on recurrent implantation failure further demonstrate the lack of advantages in live births and high …

Mecapegfilgrastim for Prophylaxis of Immunochemotherapy-Induced Neutropenia in Patients With Diffuse Large B-Cell Lymphoma: A Multicenter Pilot Trial.

BACKGROUND: This study evaluated the efficacy and safety of mecapegfilgrastim for preventing immunochemotherapy-induced neutropenia in treat … ly reduced grade ≥ 3 neutropenia; nadir neutrophil counts were higher and the need for short-acting granulocyte colony-stimulating factor was lower in the mecapegfilgrastim group (all nominal p ≤ 0.05). No cases of febrile neutropenia occurred in the mecapegfilgrastim group, compared to one case in the control group. Objective response rates were 75.0% (95% CI: 55.1, 89.3) and 57.1% ( …