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In vitro synergistic cytoreductive effects of zoledronic acid and radiation on breast cancer cells.

IntroductionBisphosphonates are mostly used in the treatment of bone metastases. They have been shown to act synergistically with other chemotherapeutic agents. It is not known, however, whether similar synergistic effects exist with radiation on breast cancer cells.MethodsHuman MCF-7 breast cancer cells were treated with up to 100 microM zoledronic acid, were irradiated with up to 800 cGy or were exposed to combinations of both treatments to determine the antiproliferative effects of zoledronic acid and radiation.ResultsZoledronic acid and radiation caused a dose-dependent and time-dependent

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… MCF-7 cells were maintained in RPMI 1640 medium supplemented with 10% heat-inactivated fetal calf serum, and with 2 mM L-glutamine, 100 μg/ml streptomycin and 100 U/ml penicillin in a 37°C incubator with a humidified air and 5% CO 2 atmosphere. The culture medium was changed twice a week. …

Antibiotics: Conventional Therapy and Natural Compounds with Antibacterial Activity-A Pharmaco-Toxicological Screening.

Antibiotics are considered as a cornerstone of modern medicine and their discovery offers the resolution to the infectious diseases problem. However, the excessive use of antibiotics worldwide has generated a critical public health issue and the bacterial resistance correlated with antibiotics inefficiency is still unsolved. Finding novel therapeutic approaches to overcome bacterial resistance is imperative, and natural compounds with antibacterial effects could be considered a promising option. The role played by antibiotics in tumorigenesis and their interrelation with the microbiota are sti

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… In 1943, Selman Waksman discovered streptomycin, the first aminoglycoside compound isolated from actinomycetes and the first antibiotic remedy for tuberculosis [ 11 ]. … The success of penicillin for the treatment of pneumonia, streptomycin for tuberculosis and chloramphenicol for typhoid fever was monumental; thus, their side effects remained almost invisible [ 45 ]. …

Expression and clinical significance of microRNA-1246 in human oral squamous cell carcinoma.

BackgroundMicroRNAs (miRNAs) are small, non-coding RNAs that may function as oncogenes or tumor suppressors. Previous studies have shown that the expression level of miR-1246 was enhanced in multiple types of cancers. However, the expression of miR-1246 in human oral squamous cell carcinoma (OSCC) and its prognostic values remain unclear.Material and methodsQuantitative reverse-transcriptase polymerase chain reaction (qRT-PCR) was used to analyze the expression of miR-1246 in 106 pairs of matched normal and tumor tissue samples. The chi-square test was used to examine the associations between

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… Cell culture The oral squamous cell carcinoma cell lines (SCC25 and CAL27) were maintained in Dulbecco’s modified Eagle’s medium (Gibco, Grand Island, NY, USA) containing 10% fetal calf serum (Gibco), 100 U/L penicillin, and 10 mg/L streptomycin. …

Тонаберсат усиливает цитотоксичность в глиобластоме с помощью темозоломида путем нарушения межклеточной связи через коннексин 43 ингибирование.

Клетки глиобластомы зависят от коннексина 43 (Cx43) на основе данных о разрыве (GJs) для межклеточной связи, позволяющей им интегрироваться в широко разветвленную злокачественную сеть. Несмотря на многообещающие перспективы новой целевой терапии, отсутствие клинически осуществимых ингибиторов GJ препятствует их внедрению в клинической практике. В настоящем исследовании мы изучили тонаберсат (ТО) — лекарство, вызывающее кровоизлияние в мозг и имеющее свойства GJ-ингиберия, с точки зрения его способности разобрать межклеточную связь в сетях глиобластомы. Для изучения функциональной межклеточной

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… cultured in DMEM/F12 medium (Gibco, Waltham, MA, USA) supplemented with EGF (0.2 μg·mL −1 ) (Biomol, Hamburg, Germany), FGF (0.1 μg·mL −1 ) (Miltenyi Biotec GmbH, Bergisch Gladbach, Germany), B27 supplement without Vitamin A (1%) (Gibco), the antifungal agent amphotericin B (2%) (Gibco) and penicillin/streptomycin … The suspension cells were induced to differentiate by transferring them into ventilated cell culture flasks (Corning™ Falcon™, New York, NY, USA) containing DMEM medium (Gibco) supplemented with Fetal Calf Serum (FCS) (10%) (Gibco) and penicillin/streptomycin (1%). …

TH1-позиция наивной CD4-T субпопуляции клеток отражает антиопухоль и аутоиммунную болезнь.

Наив CD4 T клетки традиционно рассматриваются как тщеславное, однородное, отдыхающее население, но возникающие данные свидетельствуют об их неоднородности, которая может иметь решающее значение для понимания контекста заболеваний и терапевтических последствий. В этом исследовании мы идентифицируем отдельные подгруппы как в муринных, так и в наивных наивных наивных CD4 T клеток с помощью одноклеточных, РНК-секва (scRNA-seq), с особым акцентом на подгруппе, которая выражает сверхвысокий уровень интерлейкина-7 рецептора (IL-7Rsup-hi), наряду с CD97, IL-18R и LI6C. Эта подгруппа, отсутствующая в т

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… In vitro cytotoxicity assay (CCK-8) B16F10 melanoma cells were seeded in 96-well plates with 100 μL of complete DMEM (10% FBS, 1% penicillin-streptomycin) at 37 °C, 5% CO 2 . … B16F10 and B16-OVA cells are cultured in complete DMEM (10% FBS, 1% penicillin/streptomycin). …

CD38 Is Robustly Induced in Human Macrophages and Monocytes in Inflammatory Conditions.

Macrophages and their monocyte precursors mediate innate immune responses and can promote a spectrum of phenotypes from pro-inflammatory to pro-resolving. Currently, there are few markers that allow for robust dissection of macrophage phenotype. We recently identified CD38 as a marker of inflammatory macrophages in murine in vitro and in vivo models. However, it is unknown whether CD38 plays a similar marker and/or functional role in human macrophages and inflammatory diseases. Here, we establish that CD38 transcript and protein are robustly induced in human macrophages exposed to LPS (±IFN-γ)

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… Human Monocyte Cell Lines THP-1 cells and U937 cells acquired from American Type Culture Collection (TIB-202 and CRL-10389, respectively) were cultured in complete RPMI, i.e., RPMI 1640 (Corning, Cellgro) supplemented with 10% fetal bovine serum (FBS) (Invitrogen), 1% penicillin/streptomycin (Cellgro … ), and 50 µM 2-mercaptoethanol (Sigma-Aldrich) (THP-1 cells) or with 10% FBS, 1% penicillin/streptomycin (U937 cells) at 37°C, 5% CO 2 . …

ATF3/SLC31A1-Mediated Cuproptosis Contributes to Bortezomib-Induced Peripheral Neurotoxicity and Intervention by (-)-Epigallocatechin Gallate.

Bortezomib (BTZ), the first-generation proteasome inhibitor, has been approved for the treatment of relapsed, refractory, and newly diagnosed multiple myeloma. Despite its remarkable antitumor efficacy, BTZ treatment is severely limited by a high incidence of systemic adverse reactions, primarily due to its non-selective cytotoxicity toward rapidly dividing normal cells and its potent neurotoxic effects on peripheral neurons. Bortezomib-induced peripheral neurotoxicity (BIPN) manifests as neuropathic pain and sensory abnormalities, affecting up to 31% to 64% of patients and limiting BTZ's clin

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… (Wuhan, China) with an STR document, and cultured in DMEM media (10569010, Gibco, New York, NY, USA) supplemented with 10% fetal bovine serum (16140071, Gibco, New York, NY, USA) and 1% penicillin/streptomycin. … The Mino cell lines were bought from the Procell Life Science & Technology Co., Ltd. with an STR document and cultured in RPMI-1640 (11875093, Gibco, New York, NY, USA) + 20% fetal bovine serum (16140071, Gibco, New York, NY, USA) + 1% penicillin/streptomycin, which were all in a 37 °C and 5% CO 2 incubator …

Polyphyllin I Inhibits the Metastasis of Cervical Cancer Through the Regulation of the β-Catenin Signaling Pathway.

Cervical cancer ranks as the fourth most prevalent cancer and cause of cancer-related mortality among women globally. It exhibits a recurrence/metastasis rate of approximately 30% and a dismal 5-year survival of only 17% in metastatic cases. Despite significant advancements in surgical techniques, chemoradiotherapy, and targeted therapies, effective treatment options for metastatic cervical cancer remain limited. This study explored Polyphyllin I (PPI), which is a monomeric compound derived from the Rhizoma of Paris Polyphyllin, as a potential inhibitor of cervical cancer metastasis. Mechanist

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… The HO-8910PM cell line was cultured in RPMI 1640 medium with 10% FBS, 100 U/mL penicillin, and 100 mg/mL streptomycin at 37 °C in 5% CO 2 . The SiHa and C33A cell lines were cultured in MEM medium with 10% FBS, 100 U/mL penicillin, and 100 mg/mL streptomycin at 37 °C in 5% CO 2 . 4.4. …

From Nature to Synthetic Compounds: Novel 1(N),2,3 Trisubstituted-5-oxopyrrolidines Targeting Multiple Myeloma Cells.

The insurgence of drug resistance in treating Multiple Myeloma (MM) still represents a major hamper in finding effective treatments, although over the past decades new classes of drugs, such as proteasome inhibitors and immunomodulatory drugs, have been discovered. Recently, our research team, within a Nature-Aided Drug Discovery project, isolated from Hibiscus Sabdariffa L. calyces the secondary metabolite called Hib-ester which possesses antiproliferative properties against human multiple myeloma RPMI 8226 cells, reduces migration and cell invasion and inhibits proteasome without neurotoxic

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… Cell Culture Human multiple myeloma RPMI 8226 cells were cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum, 1% L-glutamine and 1% penicillin and streptomycin (Euroclone, Italy). … Human glioblastoma U87-MG cells were cultured in DMEM low glucose supplemented with 10% fetal bovine serum, 1% L-glutamine and 1% penicillin and streptomycin (Euroclone, Italy). Cells were incubated in a humidified incubator at 37 °C and 5% CO 2 . …

Bisphosphonate Treatment Ameliorates Chemotherapy-Induced Bone and Muscle Abnormalities in Young Mice.

Chemotherapy is frequently accompanied by several side effects, including nausea, diarrhea, anorexia and fatigue. Evidence from ours and other groups suggests that chemotherapy can also play a major role in causing not only cachexia, but also bone loss. This complicates prognosis and survival among cancer patients, affects quality of life, and can increase morbidity and mortality rates. Recent findings suggest that soluble factors released from resorbing bone directly contribute to loss of muscle mass and function secondary to metastatic cancer. However, it remains unknown whether similar mech

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… Cell Lines Murine C2C12 skeletal myoblasts (ATCC, Manassas, VA) were grown in high glucose DMEM supplemented with 10% FBS, 100 U/ml penicillin, 100 mg/ml streptomycin, 100 mg/ml sodium pyruvate, 2 mM L-glutamine, and maintained at 37°C in 5% CO 2 , as shown in Pin et al. ( 40 ). … These long bones cortical preparations were then cultured ex vivo in 10% FBS and 1% penicillin/streptomycin (P/S)-αMEM for 48 h. CM was collected and stored at −20°C. …

Radiation-induced lung damage promotes breast cancer lung-metastasis through CXCR4 signaling.

Radiotherapy is a mainstay in the postoperative treatment of breast cancer as it reduces the risks of local recurrence and mortality after both conservative surgery and mastectomy. Despite recent efforts to decrease irradiation volumes through accelerated partial irradiation techniques, late cardiac and pulmonary toxicity still occurs after breast irradiation. The importance of this pulmonary injury towards lung metastasis is unclear. Preirradiation of lung epithelial cells induces DNA damage, p53 activation and a secretome enriched in the chemokines SDF-1/CXCL12 and MIF. Irradiated lung epith

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… EA.hy926, MDA-MB-231GFP_luc, and 4T1_luc cells were maintained in DMEM culture medium supplemented with 10% fetal bovine serum, 100 U/mL penicillin, 100 μg/mL streptomycin (Invitrogen, Waltham, MA, USA), and 2.5 μg/mL fungizone (Bristol-Myers, Squibb, Belgium). … Beas-2B cells were maintained in MEM culture medium supplemented with 0,05% L-glutamine (w/v), 10% fetal bovine serum, 100 U/mL penicillin, 100 μg/mL streptomycin (Invitrogen), and 2.5 μg/mL fungizone. EA.hy926, 4T1_luc and Beas-2B were incubated with 5% CO 2 . …

MGluR5 Agonist, CHPG, увеличивает число дифференцированных человеческих олигодендроцитов.

Предыдущие исследования на взрослых мышах показывают, что 2-хлор-5-гидроксифениловый гликин (CHPG) MGluR5 сокращает потери в миелине, вызванные купризоной. Этот эффект частично привязывается CHPG к рецепторам mGluR5 для реактивных астроцитов, что приводит к высвобождению нервно-партрофного фактора мозга (BDNF), что приводит к увеличению миелина и уменьшает поведенческий дефицит. Однако остается неясным, оказывает ли CHPG аналогичное благотворное воздействие на клетки человека. Для решения этой проблемы мы изучили воздействие CHPG на клетки человека, используя как индуцированные плюрипотентными

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… Penicillin-Streptomycin was omitted from N2 medium, HGF from PDGF medium, and HEPES from Glia medium. …

Targeting neuroinflammation: 3-monothiopomalidomide a new drug candidate to mitigate traumatic brain injury and neurodegeneration.

BACKGROUND: Traumatic Brain Injury (TBI) is a major risk factor for neurodegenerative disorders such as Parkinson's disease (PD) and Alzheimer's disease (AD), with neuroinflammation playing a critical role in the secondary cell death that exacerbates the initial injury. While targeting neuroinflammation holds significant therapeutic promise, clinical trials of available anti-inflammatory agents have fallen short. 3-Mono-thiopomalidomide (3-MP), a novel immunomodulatory imide drug (IMiD), was designed to curb inflammation without the adverse effects of traditional IMiDs and was evaluated across

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… This cell line was obtained from the Korean Cell Line Bank (catalog no. 30105; Seoul, Korea), grown in DMEM (supplemented with 10% FBS, penicillin 100 U/mL and streptomycin 100 μg/mL, and maintained at 37 °C and 5% CO 2 ). … These were grown in RPMI media supplemented with 10% FBS, penicillin 100 U/mL, and streptomycin 100 mg/mL, and maintained at 37 °C and 5% CO 2 . …

Combinatorial targeting of cancer bone metastasis using mRNA engineered stem cells.

BACKGROUND: Bone metastases are common and devastating to cancer patients. Existing treatments do not specifically target the disease sites and are therefore ineffective and systemically toxic. Here we present a new strategy to treat bone metastasis by targeting both the cancer cells ("the seed"), and their surrounding niche ("the soil"), using stem cells engineered to home to the bone metastatic niche and to maximise local delivery of multiple therapeutic factors. METHODS: We used mesenchymal stem cells engineered using mRNA to simultaneously express P-selectin glycoprotein ligand-1 (PSGL-1)

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… MSC were cultured in Minimum Essential Medium-α enriched with 15% FBS, 2% l -glutamine, and 1% penicillin-streptomycin solution. … Hughes (Department of Cellular and Molecular Biosciences, University of California, Irvine) and cultured in M-199 medium supplemented with 10% FBS, 2% l -glutamine, 1% penicillin-streptomycin solution, and 50 μg/mL ECGS. MDA-MB231 LucF/RFP were cultured without CO 2 . …

[Tuberculosis in Iceland. 1976].

Because of signs of tuberculous lesions in old skeletons it can be stated with certainty that tuberculosis has occurred in the country short … ttle over 90 percent. In the year 1952, when specific tuberculosis medical treatment was initiated (streptomycin, isoniazid and PAS) the death rate dropped to 14 per 100,000 population and the next year further down to 9 and since 1956 it never exceeded 5 per 100,000. From the year 1962 the tuberculosis mortality has never been over 2 per 100,000 population. T …

Negative Regulation of Cell Adhesion as a Driver of Brain Metastasis in NSCLC Patients with EGFR Amplification.

Brain metastases are strongly associated with a poor prognosis. Experimental animal models have provided valuable insights into the complex biology underlying brain metastasis, and translating these findings could pave the way for innovative management strategies for patients with brain metastases. Between May 2019 and June 2023, twenty-four lung cancer patients and thirty patients with brain metastases from lung cancer were enrolled at Guangdong Sanjiu Brain Hospital. Next-generation targeted panel sequencing (NGS) was performed on lung cancer tissue and surgical specimens from brain tumors f

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… Cells were grown in DMEM supplemented with 10% fetal bovine serum, 1% penicillin-streptomycin, and 10 µL/mL of corresponding antibiotics to ensure selection of transduced cells. Cells were kept at 37 ℃ and 5% CO 2 . All cells used for in vivo and in vitro experiments were between passages 1–10. …

Akkermansia моноколонизация модифицирует микроглию и астроциты конкретным штаммом.

Микроглиа и астроциты являются основными глиальными клетками центральной нервной системы (КНС), и их функция определяется множеством факторов. Поступили сообщения о регулировании CNS glia микробиотой, хотя роль конкретных бактерий не была определена. Мы колонизировали мышей, свободных от микробов, с штаммом Akkermansia muciniphila (AmT) и романом A. muciniphila штамм BWH-H3 (Am-H3), изолированным от предмета с множественным склерозом, и по сравнению с мышами, колонизированными с штаммом BWH-E5 (Bc), изолированными от здорового контрольного предмета. Затем мы изучили влияние этих бактерий на ми

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… were homogeized and passed through a 70 μm mesh filter on a 96-well culture plate containing complete media composed of: v:v; Iscove’s modified dulbecco’s medium (Gibco, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (FBS; Thermo Fisher Scientific, Waltham, MA, USA), 1% penicillin/streptomycin …

The Synthetic Collagen-Binding Peptide NIPEP-OSS Delays Mouse Myeloma Progression.

Multiple myeloma (MM) is the second most common hematological malignancy. It is a clonal B-cell disorder characterized by the proliferation of malignant plasma cells in the bone marrow, the presence of monoclonal serum immunoglobulin, and osteolytic lesions. An increasing amount of evidence shows that the interactions of MM cells and the bone microenvironment play a significant role, suggesting that these interactions may be good targets for therapy. The osteopontin-derived collagen-binding motif-bearing peptide NIPEP-OSS stimulates biomineralization and enhances bone remodeling dynamics. Due

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… They were cultured at 37 °C at 5% CO 2 in RPMI 1640 medium supplemented with 15% fetal bovine serum (FBS) + 1× penicillin/streptomycin + 1× Glutmax (Gibco, Life Technologies, Carlsbad, CA, USA). …

Knockdown of lncRNA AL928768.3 inhibits multiple myeloma cell proliferation by inducing cell cycle arrest in G0/G1 phase.

BackgroundMultiple myeloma (MM) is a B-lymphocyte-derived malignancy. It ranks as the second most common hematological malignancy, with relatively high morbidity and mortality. However, the molecular mechanisms of MM occurrence and development remain elusive. This study found that long non-coding RNA AL928768.3 (lncRNA AL) was abnormally expressed in MM samples. However, the effect and molecular mechanism of lncRNA AL on the occurrence and development of MM remains unclear.MethodsBone marrow fluids of MM patients (n=54) and volunteers (n=13) were collected and CD138+ cells were isolated. The e

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… The cells were cultured in RPMI-1640 medium (10% fetal bovine serum) containing 100 U/mL penicillin and 100 mg/mL streptomycin and maintained in a 37 °C incubator with 5% CO 2 and saturated humidity. …

Bisphosphonate-related osteonecrosis. Application of adipose-derived stem cells in an experimental murine model.

BACKGROUND: Bisphosphonate-related osteonecrosis of the jaw is a pathological condition without effective established treatment and preventive strategies. The aim of this study was to analyse the effect of adipose-derived stem cells (ASC) in an experimental murine model of osteonecrosis. MATERIAL AND METHODS: 38 Wistar rats were injected intraperitoneally with zoledronic acid. After treatment, upper jaw molars were extracted. The animals were randomly assigned to one of two groups. In the control group, saline solution was applied over the alveolar sockets after the tooth extractions. In the

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… After filtering through a 70-µm nylon mesh, the cells were cultured in DMEM (Gibco BRL) containing 10% FBS (Gibco BRL) and 1% penicillin-streptomycin (Gibco BRL). The medium was changed to remove nonadherent cells 24 h after seeding and every 3–4 days thereafter. …