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Selection of attenuated dengue 4 viruses by serial passage in primary kidney cells. V. Human response to immunization with a candidate vaccine prepared in fetal rhesus lung cells.

A dengue 4 (strain H241, PDK35-TD3 FRhL p3) vaccine attenuated by passage in primary dog kidney cells followed by passage and final vaccine preparation in DBS-FRhL-2 cells was tested in five yellow fever-immune volunteers. Only two volunteers seroconverted by producing hemagglutination-inhibiting and neutralizing antibodies. Mild illness, compatible with dengue infection was found only in the individuals who later developed antibodies. Both volunteers developed a rash by the 8th day following vaccination, coinciding with a slight elevation in temperature and leukopenia. Additionally, several s…

Infantile gastroenteritis: a clinical study of reovirus-like agent infection.

In a clinical study of 32 infants with symptoms from infections with the human reovirus-like agent (R.I.A.) identified by electron microscopy (E.M.) of faecal extracts, a fairly consistent clinical pattern was found in 30 who had a gastroenteritis-like illness. The disease was usually mild, affecting mainly infants less than 2 years and males more commonly than females. The incubation period appeared to be 48-72 hours; and the onset was sudden, often with vomiting in the first 1-2 days of the illness. Loose yellow-green offensive stools without blood or mucus developed after a variable time, a

The impact of insecticide-resistance on control of vectors and vector-borne diseases.

A questionnaire inquiring into the nature of schemes for the insecticidal control of disease vectors, the development of resistance in these … rely affected control both of many insect species and of the diseases they transmit (e.g., malaria, yellow fever, filariasis, typhus, plague). Certain other public health problems (onchocerciasis, Chagas' disease, trypanosomiasis, leishmaniasis) have not so far been greatly affected by resistance, but it is difficult to be sure of the continued reliability of …

Identification of multiple arbovirus infections/exposure in Northeastern Brazil using a multiplex microsphere immunoassay.

Several mosquito-borne arboviruses, including dengue (DENV), Zika (ZIKV), West Nile (WNV), yellow fever (YFV), and chikungunya (CHIKV) viruses, have caused disease outbreaks of public health concern in the tropics and subtropics. The overlap distribution of these arboviruses and cross-reactivities of antibodies to orthoflavivirus envelope protein underscore the need for a reliable and convenient serological test to distinguish these arboviruses in endemic countries. We developed a multiplex IgG microsphere immunoassay (MIA) using 13 antigens to test 8 panels of samples with well-documented arb…

High Aedes spp. larval indices in Kinshasa, Democratic Republic of Congo.

BACKGROUND: Dengue, yellow fever, chikungunya and Zika are among the most important emerging infectious vector-borne diseases worldwide. In the Democratic Republic of Congo (DRC), increases in cases of dengue and outbreaks of yellow fever and chikungunya have been reported since 2010. The main vectors of these arboviruses, Aedes aegypti and Aedes albopictus, have been reported in DRC, but there is a lack of detailed information on their presence and spread to guide disease control efforts. METHODS: In 2018, two cross-sectional surveys were conducted in Kinshasa province (DRC), one in the rain…

The 2014 FIFA World Cup: communicable disease risks and advice for visitors to Brazil--a review from the Latin American Society for Travel Medicine (SLAMVI).

The next FIFA World Cup will be held in Brazil in June-July 2014. Around 600,000 international visitors and participants (as well over 3 mil … enge. Dengue poses a significant risk in all states, including the host cities. Vaccination against yellow fever is recommended except for travelers who will only visit coastal areas. Travelers visiting high-risk areas for malaria (Amazon) should be assessed regarding the need for chemoprophylaxis. Chikunguya fever may be a threat for Brazil, given the presenc …

Rapid laboratory confirmation of the 2025 Sudan Virus Disease (Svd) index case in Uganda.

BACKGROUND: On January 30, 2025, the Uganda Ministry of Health declared an outbreak of Sudan virus disease (SVD) following laboratory confir … ) confirmation, result validation, and reporting. RESULTS: The sample tested negative for Marburg, Yellow fever, Rift Valley fever, and Crimean-Congo Hemorrhagic Fever viruses. However, it tested positive for SUDV. Four parallel tests utilizing the Real Star® Filovirus Screen RT-PCR Kit 1.0 (Altona Diagnostics GmbH, Hamburg, Germany) on CFX96 Bio-Rad platform …

Participation of 14-3-3ε and 14-3-3ζ proteins in the phagocytosis, component of cellular immune response, in Aedes mosquito cell lines.

BACKGROUND: Better knowledge of the innate immune system of insects will improve our understanding of mosquitoes as potential vectors of diverse pathogens. The ubiquitously expressed 14-3-3 protein family is evolutionarily conserved from yeast to mammals, and at least two isoforms of 14-3-3, the ε and ζ, have been identified in insects. These proteins have been shown to participate in both humoral and cellular immune responses in Drosophila. As mosquitoes of the genus Aedes are the primary vectors for arboviruses, causing several diseases such as dengue fever, yellow fever, Zika and chikunguny…

A community-based prospective cohort study of dengue viral infection in Malaysia: the study protocol.

BACKGROUND: Globally, dengue infections constitute a significant public health burden. In recent decades, Malaysia has become a dengue hyper … dengue infection, vaccination history against other flaviviruses such as Japanese encephalitis and Yellow fever, and the family history of dengue infection will also be collected. In addition, a mosquito surveillance will be carried out simultaneously in recruitment areas to determine the molecular taxonomy of circulating vectors. DISCUSSION: The research fi …

Vaccine Platforms to Control Arenaviral Hemorrhagic Fevers.

Arenaviruses are rodent-borne emerging human pathogens. Diseases caused by these viruses, e.g., Lassa fever (LF) in West Africa and South Am … acting vaccine or post-exposure prophylaxis would be best. The 2(nd) vaccine technology is based on Yellow Fever (YF) 17D vaccine. We designed YF17D-based recombinant viruses expressing LASV glycoproteins (GP) and showed protective efficacy of these recombinants. In the current study we developed a novel technology to clone LASV nucleocapsid within YF17D C gen …

Advances in mosquito olfaction: Genetic, genomic, and behavioral approaches in Aedes aegypti.

Mosquito behavior has long been studied because of its importance in the spread of human disease. More recently, mosquitoes have emerged as a model for studying olfaction, given their exquisite ability to locate human hosts. Host seeking unfolds through a series of behavioral phases, including activation, flight, landing, blood feeding, and oviposition, making it well suited for study across multiple levels. In this review, we focus on methodological advances that have shaped the study of mosquito host-seeking behavior, with a focus on the Yellow Fever mosquito, Aedes aegypti. We highlight thr…

Dissecting the host determinants of orthoflavivirus infection using QIC-seq.

Orthoflaviviruses are genetically related, yet cause distinct disease patterns ranging from hepatitis and vascular shock syndrome to encepha … challenge. Using a CRISPR library focused on select ER-proteostasis genes, we show that dengue and yellow fever viruses are strictly dependent on subunits of the oligosaccharyltransferase complex, while the more distantly related West Nile and Langat viruses are dependent on components of the ER-associated degradation machinery. Our data further shows virus-i …

Advances and Challenges in Vaccine Development for West Nile Virus (WNV) Infection.

West Nile Virus (WNV) belongs to the orthoflavivirus genus and is part of the Flaviviridae family, which includes the Japanese encephalitis virus, Dengue virus, Zika virus, and yellow fever virus. WNV circulates among birds and mosquitoes, posing infection risks to humans and mammals. The significant rise in WNV's geographic spread and infection rates over the past five decades has prompted urgent public health concerns, driving the need for accelerated vaccine research. The development of a vaccine for WNV infection presents several challenges, primarily due to the virus's complex biology, th…

Факторы, связанные с присутствием COVID-19 среди педиатрического населения в больницах: тематическое исследование.

ЦЕЛЬ: Анализ факторов, связанных с присутствием COVID-19 среди педиатрического населения в больницах. МЕТОД: Это было семейное исследование, проведенное с медицинскими записями детей в возрасте до 14 лет. Считается, что педиатрическая популяция с положительным тестом COVID-19, а педиатрическая популяция с отрицательным тестом COVID-19 - контрольным показателем. В каждом случае использовался контроль, общий объем которого составил 486 медицинских записей. Был проведен деформационный анализ, бивариатный анализ и логистическая регрессия.Результаты: К числу переменных, связанных с появлением COVID

Potential distribution of Aedes vittatus as an invasive species in North America.

The recent detection of populations of the mosquito Aedes vittatus in the Dominican Republic and Cuba has raised concerns over its potential for broader invasion in the Western Hemisphere, and particularly on the North American mainland. This species has been associated with the maintenance and transfer of yellow fever, Zika, chikungunya, and dengue viruses on its native distributional area. A previous study identified parts of North America with environments apparently suitable for the species, but different modeling approaches can give different results. Using models calibrated across the re…

Assessment of flavivirus RNA stability and infectivity in various water environments.

INTRODUCTION: Flaviviruses such as dengue virus (DENV), Zika virus (ZIKV), Japanese encephalitis virus (JEV), and Yellow fever virus (YFV) are mosquito-borne RNA viruses causing major public health threats in major parts of the world. While DENV and ZIKV have been detected in urine samples, data on the presence and stability of flaviviruses in the water environment are limited. METHODS: In this study, we determined the stability and infectivity of flavivirus in different water environments by utilizing RT-qPCR and plaque assay to explore the feasibility of environmental detection and surveill…

Метод многокритерийного анализа решений для дифференциального диагноза тропических фебрильных заболеваний.

В настоящем документе используется аналитический иерархический процесс (AHP) для повышения точности дифференциального диагноза женских заболеваний, особенно в тропических регионах, где неправильное диагностирование может иметь серьезные последствия. Миграция работников здравоохранения из развивающихся стран привела к тому, что прифронтовые медицинские работники (ПЗС) использовали неадекватные протоколы для диагностики сложных медицинских условий. В рамках этого исследования была внедрена новаторская система поддержки принятия решений по медицинским вопросам (ССРЗ), в которую были включены факт

Совпадение в аннотации оригинала
This paper employs the Analytical Hierarchy Process (AHP) to enhance the accuracy of differential diagnosis for febrile diseases, particular … from 85.4% to 96.9% for various diseases, surpassing physicians' predictions for Lassa, Dengue, and Yellow Fevers. The MDSS is recommended for use by FHWs in communities lacking medical experts, facilitating timely and precise diagnoses, efficient application of diagnostic test kits, and reducing overhead expenses for administrators.

Acute Fatty Liver of Pregnancy: A Diagnostic Challenge.

Acute fatty liver of pregnancy (AFLP) is a rare, catastrophic disease affecting women in the third trimester of pregnancy or the postpartum period. We report a case of 24-year-old G2A1 with GA of 35 weeks who presented with amenorrhea, nausea, fever, vomiting, headache and yellowness of eyes. The patient was diagnosed with severe preeclampsia with Intrauterine death (IUD) with hemolysis elevated liver enzymes low platelets (HELLP). Investigations showed hypoglycemia, low platelet count, and raised liver enzymes with an altered coagulation profile. The patient was kept in the Medicine Intensive

Terpenic Constituents of Essential Oils with Larvicidal Activity against Aedes Aegypti: A QSAR and Docking Molecular Study.

Aedes aegypti is a vector for the arbovirus responsible for yellow fever, Zika and Chikungunya virus. Essential oils and their constituents are known for their larvicidal properties and are strong candidates for mosquito control. This work aimed to develop a quantitative structure-activity study and molecular screening for the search and design of new larvicidal agents. Twenty-five monoterpenes with previously evaluated larvicidal activity were built and optimized using computational tools. QSAR models were constructed through genetic algorithms from the larvicidal activity and the calculation…