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Pregnancy Deciduosis Mimicking Abdominal Tuberculosis and Peritoneal Carcinomatosis: A Case Report.

Background and clinical significance: Gestational deciduosis is a benign, progesterone-related ectopic decidual reaction that commonly invol … abundant eosinophilic cytoplasm in a perivascular and septal distribution. The cells showed diffuse vimentin, CD10, and progesterone-receptor expression and lacked epithelial and mesothelial marker expression, supporting diffuse peritoneal deciduosis. The postoperative course was uncomplicated, and pelvic ultrasonography at 40 days postpartum showed no res …

Gastroblastoma: a case report and literature review.

OBJECTIVE: Gastroblastoma (GB) is an extremely rare biphasic malignant embryonal tumor of the stomach, primarily seen in children, with limi … ntiation. Immunohistochemistry (IHC) results showed positivity for Pan-Cytokeratin (PCK), CD56, and Vimentin, with a low Ki-67 index (5%). A final diagnosis of GB (pT4aN2aM0) was confirmed. The patient did not receive adjuvant therapy postoperatively and showed no signs of tumor recurrence or metastasis at 14 months of follow-up. CONCLUSION: The diagnosis …

Resolution of Recurrent Severe Hypoglycemia After Surgical Excision of a Primary Hepatic Neoplasm of Uncertain Histogenesis in a Dog: A Case Report.

Primary hepatic neoplasms in dogs, while uncommon, are occasionally associated with severe hypoglycemia, although the underlying mechanism m … asional cytoplasmic vacuolation, and no unequivocal lipoblasts. The neoplastic cells showed diffuse vimentin immunoreactivity, weak cytoplasmic S-100 immunoreactivity in a subset of cells, and no immunoreactivity for smooth-muscle actin or hepatocyte paraffin 1. These findings raised the possibility of adipocytic differentiation but were insufficient to es …

Построение олигодендроцитов, подобных прекурсорам, из эмбриональных фибробатластов мыши с использованием miR-7a Имитация.

ЦЕЛИ: Разработка эффективных стратегий повышения эффективности мер по ликвидации последствий по-прежнему является одной из основных терапевтических проблем в связи с такими демиелинизирующими расстройствами, как множественный склероз (МС). МикроРНК (миРНК) стали мощным регулятором клеточной судьбы. Было показано, что miR-7a , в значительной степени выражается в олигодендроцитных клетках-прекурсорах (ОПК). Цель этого исследования заключалась в изучении вопроса о том, является ли завышенное выражение мнений miR-7a :: может перепрограммировать эмбриональные фибробатласты мыши (МЭФ) в похожие на O

Совпадение в аннотации оригинала
OBJECTIVES: Developing effective strategies to enhance remyelination remains a major therapeutic challenge in demyelinating disorders such a … -like cells and oligodendrocytes in vitro. MATERIALS AND METHODS: MEFs were cultured, confirmed by vimentin immunostaining, and transfected with miR-7a mimics using LipofectamineTM 3000 or LipofectamineTM RNAiMAX reagent. Transfection efficiency was assessed by qRT-PCR analysis of miR-7a expression levels. Cells were then cultured in OPC induction medium …

Super-enhancer-associated LINC00963 promotes metastasis of gastric cancer through epithelial-mesenchymal transition.

BACKGROUND: In clinical practice, gastric cancer (GC) is a common malignancy with high morbidity. Accumulating research has revealed that ln … ZEB1 proteins, and the protein expression levels of several marker proteins related to EMT, such as Vimentin, N-cadherin were observed to decrease (Fig 1). CONCLUSION: This study demonstrated that the super-enhancer-associated LINC00963 promoting tumor metastasis in gastric cancer through EMT.

A facile liquid biopsy assay for highly efficient CTCs capture and reagent-less monitoring of immune checkpoint PD-L1 expression on CTCs with non-small cell lung cancer patients.

Programmed cell death 1 ligand 1 (PD-L1) immunotherapy holds a pivotal role in lung cancer treatment. However, current methods for monitorin … cer (NSCLC) patients. This assay was achieved by fabricating two high-performance probes: EpCAM and Vimentin dual-aptamer modified nitrogen-doped carbon quantum dots probe (E/V-apt-N-CQDs) and hairpin PD-L1 aptamer coupled with gold nanoparticles (PD-L1-apt-AuNPs). The E/V-apt-N-CQDs probe effectively captured two types of CTC models (H1299 and A549) exhib …

SARS-CoV-2 infection disrupts syncytial and endothelial integrity and alters PLGF levels in the placenta.

INTRODUCTION: SARS-CoV-2 infection during pregnancy has been associated with an increased risk for several pregnancy-related disorders, part … AA levels were observed. Finally, SARS-CoV-2-infected placentas exhibited a significant decrease in vimentin expression. DISCUSSION: SARS-CoV-2 infection negatively impacts placental integrity in the form of increased syncytial knots, dysregulated RAS components, and endothelial damage. Since all these features are similarly disrupted in PE, this could be …

The therapeutic effect of zinc oxide nanoparticles against lipopolysaccharides on the rats' retina.

BACKGROUND: Lipopolysaccharide (LPS), a potent endotoxin, triggers oxidative stress and inflammatory cascades that contribute to retinal deg … tochemically for markers of oxidative stress, inflammation, and apoptosis (including PCNA, p53, and vimentin), while gut microbiota composition was assessed by 16S rRNA sequencing. RESULTS: LPS exposure caused marked retinal degeneration, elevated oxidative and inflammatory markers (mTOR, COX-2, Caspase-3, MDA, and NO), and significant gut microbiota dysb …

Clinicopathological and molecular features of so-called low-grade oncocytic fumarate hydratase-deficient renal cell carcinoma: a study of 5 cases.

Low-grade oncocytic fumarate hydratase-deficient renal cell carcinoma (FHdRCC), resembling succinate dehydrogenase-deficient RCC morphologic … , while the remaining cases exhibited both FH and 2SC positivity. All cases were positive for PAX8, Vimentin, P504s, and SDHB, but negative for CD117, CA9, CK20, ALK, and cathepsin-K. CK7 and TFE3 positivity were seen in 2 and 4 of 5 cases, respectively. The whole exome sequencing and multiplex PCR-based next generation sequencing identified pathogenic or …

YWHAG mediated epithelial-mesenchymal transition facilitates tumor progression and metastatic spread in non-small cell lung cancer.

BACKGROUND: YWHAG, a 14-3-3 protein family member, has been implicated in tumorigenesis, yet its role in lung cancer progression and underly … YWHAG. Mechanistically, YWHAG knockdown suppressed EMT (increased E-cadherin, decreased N-cadherin/Vimentin), proliferation, and migration, while overexpression exerted opposite effects. YWHAG interacted with LRRK2 to activate the PI3K/AKT pathway, and LRRK2 overexpression rescued PI3K/AKT suppression post-YWHAG knockdown. In mice, lung-specific YWHAG kno …

Effect of two artificial photoperiods on male domestic cat reproduction.

A thorough understanding of the effect of exogenous light could offer a natural method for reproduction manipulation in the domestic cat. Th … centage was higher in SP in both the nucleus (P < 0.05) and cytoplasm (P < 0.05). No differences in vimentin immunoreactivity were found between groups.

Pentagalloyl glucose reverses chemoresistance in triple-negative breast cancer via EMT inhibition and miRNA modulation.

Triple-negative breast cancer (TNBC) presents a major clinical challenge due to its aggressive behavior, limited targeted therapies, and fre … EMT reversal effect was observed through upregulation of E-cadherin and downregulation of ZEB1 and vimentin, accompanied by reduced cell migration and invasion. miRNA profiling revealed the upregulation of miR-200c-3p and other tumor-suppressive miRNAs. Notably, overexpression of miR-200c-3p alone enhanced chemosensitizing and DOX-induced apoptosis in MDA …

MBD2 promotes epithelial-to-mesenchymal transition (EMT) and ARDS-related pulmonary fibrosis by modulating FZD2.

OBJECTIVE: To investigate the role and underlying mechanism of Methyl-CpG binding domain protein 2 (MBD2) in the pathogenesis of acute respiratory distress syndrome (ARDS)-related pulmonary fibrosis. METHODS: Murine models for ARDS-related pulmonary fibrosis were established in wildtype or MBD2 knockout mice, expressions of MBD2 were determined with immunohistochemistry (IHC), immunofluorescence, and western blot. Epithelial-to-mesenchymal transition (EMT) was detected with determined with decreased expression of E-cadherin and increased expressions of N-cadherin, Vimentin, and α-smooth muscl…

Pulmonary fibrosis in post-COVID-19: epithelial-mesenchymal transition as a key mechanism and target for therapy.

Pulmonary fibrosis is a serious threat to global health, especially in after math of covid-19 infection, eventually results in fibrotic remo … e TGF-β/Smad, NF-κB pathways that maintain EMT via downregulation of E-Cadherin and upregulation of vimentin, fibronectin and α-SMA. SARS-CoV spike-protein binds to AEC-2 cells initiates cytokine storm followed by amplified NF-κB pathway, and oxidative stress. Elevated activation of this pathway increases Snail, Slug, Twist expression that leads to EMT. Mo …

Targeting the lncRNA GAS5/TLR4/NLRP3 signaling cascade inhibits endometrial stromal cell pyroptosis and prevents the progression of intrauterine adhesions.

Intrauterine adhesion (IUA) poses a serious threat to women's health, and its specific pathogenesis has not yet been elucidated. Our study f … wn of GAS5 improved endometrial injury and uterine adhesions, decreased the levels of TIMP1, α-SMA, Vimentin, and COL1A1, but elevated MMP9 level to reduce excessive accumulation of extracellular matrix (ECM), and inhibited the expression of NLRP3, cleaved caspase-1, GSDMD, and nuclear p65 to ameliorate pyroptosis in IUA model mice. As confirmed by bioinfo …

In vitro and in silico investigation of the critical role of GABAB receptor activation in human breast cancer and its natural agonistsIn vitro and in silico investigation of the critical role of GABAB receptor activation in human breast cancer and its natural agonists.

γ-aminobutyric acid type B (GABAB) receptors, a class of G protein-coupled receptors, serve pivotal roles in modulating cellular signaling p … d clonogenicity in a dose-dependent manner. Moreover, baclofen treatment led to a downregulation of vimentin expression, whereas the expression of β-catenin was upregulated, indicating suppression of EMT in MDA-MB-231 cells. Notably, the phosphorylation levels of Akt and ERK1/2 remained unaltered, suggesting that the inhibitory effects mediated by baclofen …

Levosimendan mitigates renal fibrosis via TGF-β1/Smad axis modulation in UUO rats.

Chronic kidney disease (CKD) is characterized by kidney fibrosis involving epithelial-mesenchymal transition (EMT), and extracellular matrix … fibrosis in LVS-treated rats. Moreover, LVS treatment remarkably downregulated the levels of α-SMA, vimentin, p-Smad 2/3 and upregulated E-cadherin in UUO rats, decreased Smad 4, collagen I, β-catenin, and MMP-7-mediated ECM and increased Smurf 2 and Smad 7 in NRK-52E cells. LVS inhibits EMT and ECM turnover via TGF-β1/Smad axis modulation, highlighting th …

Therapeutic potential of tadalafil in acetic acid-induced gastric ulcer in rats: mechanisms and outcomes.

Gastric ulcers, a prevalent gastrointestinal disorder marked by mucosal damage, sometimes resist complete healing with existing therapies. T … n, and the expression of nuclear factor kappa B (NF-κB, a protein complex regulating inflammation), vimentin, and proliferating cell nuclear antigen (PCNA, a marker of cell proliferation). Additionally, mRNA levels of AMP-activated protein kinase (AMPK), endothelial nitric oxide synthase (eNOS), and inducible nitric oxide synthase (iNOS), along with tissue …

Harmine attenuates renal fibrosis via Twist1 suppression: A novel anti-fibrotic strategy for chronic kidney disease with efficacy/safety profiling.

Renal fibrosis, a pathological hallmark of chronic kidney disease (CKD), accounts for over 40 % of end-stage renal disease cases globally, w … fibrosis. In vitro, Harmine dose-dependently suppressed hypoxia-induced EMT in HK-2 cells, reducing vimentin expression and restoring E-cadherin at 5 μmol/L and 10 μmol/L. In murine models, Harmine (2.5, 5, 10 mg/kg/day) attenuated renal fibrosis in unilateral ischemia-reperfusion injury (uIRI) and unilateral ureteral obstruction (UUO) models. Safety profi …

Circ_0090100 induces AHNAK expression to inhibit trophoblast cell proliferation and invasion and accelerate cell apoptosis by segregating miR-139-5p in preeclampsia.

The etiology of preeclampsia (PE) is complex and is known to involve the expression of circular RNAs (circRNAs). Among these, the function o … , respectively. Western blot analysis was performed to detect the protein expression of N-cadherin, Vimentin, and AHNAK. Dualluciferase reporter assay and RNA immunoprecipitation (RIP) assay were conducted to elucidate the relationships between circ_0090100, miR-139-5p, and AHNAK. We found that the expression of circ_0090100 and AHNAK was upregulated, whil …