Результаты поиска

Supplementing Ca salts of soybean oil to late-gestating beef cows: impacts on performance and physiological responses of the offspring.

This experiment compared the performance and physiological responses of the offspring from cows supplemented with Ca salts of soybean oil (C … atty acid-binding protein and stearoyl-CoA desaturase) and myogenic (myogenic differentiation 1 and myogenin) genes in the longissimus muscle (LM) compared with CON. No treatment differences in birth BW, weaning BW, and final preconditioning BW were noted (P ≥ 0.36). Average daily gain and final BW in the feedyard were greater (P ≤ 0.05) in steers from CSS …

The phosphodiesterase-4 inhibitor roflumilast reverts proteolysis in skeletal muscle cells of patients with COPD cachexia.

Peripheral muscle weakness and mass loss are characteristic features in severe chronic obstructive pulmonary disease (COPD). We hypothesized … r-1 was upregulated, whereas that of myocyte-specific enhancer factor 2C, myogenic differentiation, myogenin, myostatin, atrogin-1, and muscle RING-finger protein-1 was downregulated. In myotubes of severe COPD patients with cachexia, roflumilast-induced cAMP signaling exerts beneficial effects by targeting muscle protein breakdown (tyrosine release), alon …

Parotid gland solitary fibrous tumor: a case report and clinicopathologic review of 22 cases from the literature.

Solitary fibrous tumors (SFTs) are rare tumors in the head and neck, and even more so in the parotid gland. The mass-like clinical presentat … S100 protein, cytokeratin, EMA, CAM5.2, smooth muscle actin, muscle specific actin, desmin, MYOD1, myogenin, CD117, GFAP, CD31, FVIII-RAg, collagen IV, p63, p53, calponin, caldesmon, CD56, NFP, and ALK-1. The principle differential diagnoses include pleomorphic adenoma, myoepithelioma, nodular fasciitis, schwannoma, fibromatosis coli, spindle cell "sarcom …

Fasudil improves survival and promotes skeletal muscle development in a mouse model of spinal muscular atrophy.

BACKGROUND: Spinal muscular atrophy (SMA) is the leading genetic cause of infant death. It is caused by mutations/deletions of the survival motor neuron 1 (SMN1) gene and is typified by the loss of spinal cord motor neurons, muscular atrophy, and in severe cases, death. The SMN protein is ubiquitously expressed and various cellular- and tissue-specific functions have been investigated to explain the specific motor neuron loss in SMA. We have previously shown that the RhoA/Rho kinase (ROCK) pathway is misregulated in cellular and animal SMA models, and that inhibition of ROCK with the chemical

[Effect of electroacupuncture on amyotrophia and expression of myogenic differentiation-related genes of gastrocnemius in rats with chronic constriction injury of sciatic nerve].

OBJECTIVE: To explore the effect of electroacupuncture(EA) on amyotrophia and expression of paired box7(Pax7), myogenic differentiation antigen-1 (Myod1), myogenin (Myog), myosin heavy chain- Ⅱa (Myh2), myosin heavy chain-Ⅱx (Myh1) and myosin heavy chain-Ⅰ (Myh7) of denervated gastrocnemius in rats with chronic constriction injury (CCI) of the right sciatic nerve, so as to explore its mechanisms underlying postponing development of amyotrophia. METHODS: Sixty-six SD adult male rats were randomly divided into sham operation (sham) group (n＝24), model group (n＝24) and EA group (n＝18). The dener…

Aging-related hyperphosphatemia impairs myogenic differentiation and enhances fibrosis in skeletal muscle.

BACKGROUND: Hyperphosphatemia has been related to the development of sarcopenia in aging mice. We describe the intracellular mechanisms involved in the impairment of the myogenic differentiation promoted by hyperphosphatemia and analyse these mechanisms in the muscle from older mice. METHODS: C2 C12 cells were grown in 2% horse serum in order to promote myogenic differentiation, in the presence or absence of 10 mM beta-glycerophosphate (BGP) for 7 days. Troponin T, paired box 7 (Pax-7), myogenic factor 5 (Myf5), myogenic differentiation 1 (MyoD), myogenin (MyoG), myocyte enhancer factor 2 (ME…

[DICER1-mutant primary intracranial sarcoma: analysis of five cases].

Objective: To investigate the clinicopathological characteristics and differential diagnosis of DICER1-mutant primary intracranial sarcoma. … of vimentin and variable expression of myogenic marker (desmin), with or without focal MyoD1 and/or Myogenin expression. Four tumors exhibited diffuse, strong expression of TLE1 and p53, while only three tumors showed loss of ATRX (nuclear) expression. Two cases showed mosaic loss of H3K27me3 expression in neoplastic cells. The Ki-67 proliferation index wa …

Selective Small-Molecule AdipoR1 Agonist 3-Hydroxy Pterocarpan Salt (CDRI-1709S) Ameliorates Skeletal Muscle Atrophy.

BACKGROUND: Skeletal muscle atrophy is a frequent comorbidity of metabolic disorders and chronic diseases, and despite its high prevalence, … 2C12 myoblast differentiation into mature myotubes, accompanied by increased expression of MyoD and myogenin (p < 0.05). Treated myotubes were protected against cytokine-, Dex- and nutrient-deprivation-induced atrophy through suppression of atrogenes Atrogin-1 and MuRF-1 (p < 0.01), restoration of myogenic markers (p < 0.05) and prevention of Dex-induced f …

Androgen-dependent pathology demonstrates myopathic contribution to the Kennedy disease phenotype in a mouse knock-in model.

Kennedy disease, a degenerative disorder characterized by androgen-dependent neuromuscular weakness, is caused by a CAG/glutamine tract expansion in the androgen receptor (Ar) gene. We developed a mouse model of Kennedy disease, using gene targeting to convert mouse androgen receptor (AR) to human sequence while introducing 113 glutamines. AR113Q mice developed hormone and glutamine length-dependent neuromuscular weakness characterized by the early occurrence of myopathic and neurogenic skeletal muscle pathology and by the late development of neuronal intranuclear inclusions in spinal neurons.

Isolation of mesenchymal stem cells from shoulder rotator cuff: a potential source for muscle and tendon repair.

The self-healing potential of each tissue belongs to endogenous stem cells residing in the tissue; however, there are currently no reports mentioned for the isolation of human rotator cuff-derived mesenchymal stem cells (RC-MSCs) since. To isolate RC-MSCs, minced rotator cuff samples were first digested with enzymes and the single cell suspensions were seeded in plastic culture dishes. Twenty-four hours later, nonadherent cells were removed and the adherent cells were further cultured. The RC-MSCs had fibroblast-like morphology and were positive for the putative surface markers of MSCs, such a

Pharmacological hypogonadism impairs molecular transducers of exercise-induced muscle growth in humans.

BACKGROUND: The relative role of skeletal muscle mechano-transduction in comparison with systemic hormones, such as testosterone (T), in reg … 3 fold, P = 0.0005, ES = 0.72; IGF-1Ec: Z: 2 fold, P > 0.99 vs. P: 4.7 fold, P = 0.0005, ES = 0.68; myogenin: Z: 1.3 fold, P > 0.99 vs. P: 2.7 fold, P = 0.002, ES = 0.72), RNA/DNA (Z: 0.47 ± 0.03 to 0.53 ± 0.03, P = 0.31 vs. P: 0.50 ± 0.01 to 0.64 ± 0.04, P = 0.003, ES = 0.72), and RNA/ASP (Z: 5.8 ± 0.4 to 6.8 ± 0.5, P > 0.99 vs. P: 6.5 ± 0.2 to 8.9 ± 1.1, …

Adult-type fibrosarcoma: A reevaluation of 163 putative cases diagnosed at a single institution over a 48-year period.

Adult-type fibrosarcoma (FS) was once considered the most common adult sarcoma, but is now considered a diagnosis of exclusion. No recent se … CK, S100, Melan A, HMB-45, CD34, TLE1, CD31, HHV8, smooth muscle actin, desmin, ALK1, CD99, Myo-D1, myogenin, c-kit, INI1, CD21, p63, calretinin, WT1, and TTF1. Fluorescence in situ hybridization analysis for SYT gene rearrangement was done in 6 putative CK-negative synovial sarcomas (SS). Revised diagnoses were based on clinical, morphologic, IHC, and mol …

Umbelliferone attenuates diabetic sarcopenia by modulating mitochondrial quality and the ubiquitin-proteasome system.

BACKGROUND: Sarcopenia, characterized by the progressive loss of skeletal muscle mass, strength, and physical performance, is traditionally … function in diabetes. Umbelliferone significantly upregulated key myogenic markers, including MyoD, myogenin, Myh2, and skeletal muscle myosin, thereby promoting myoblast differentiation into myotubes. In diabetic mice, umbelliferone treatment enhanced grip strength and lean mass, highlighting its potential to restore overall muscle function. Furthermore, …

Functions and Therapeutic Potentials of Long Noncoding RNA in Skeletal Muscle Atrophy and Dystrophy.

Skeletal muscle is the most abundant tissue in the human body and is responsible for movement, metabolism, energy production and longevity. Muscle atrophy is a frequent complication of several diseases and occurs when protein degradation exceeds protein synthesis. Genetics, ageing, nerve injury, weightlessness, cancer, chronic diseases, the accumulation of metabolic byproducts and other stimuli can lead to muscle atrophy. Muscular dystrophy is a neuromuscular disorder, part of which is caused by the deficiency of dystrophin protein and is mostly related to genetics. Muscle atrophy and muscular

Совпадение в полном тексте источника
… LncRNA Myoparr is transcribed from the upstream region of the myogenin gene. It has similar expression patterns but divergent promoters [ 62 ]. Myoparr is enriched only in the nucleus. Interference with Myoparr significantly reduces the level of myogenin. … Knockdown of Myoparr significantly reduces the myogenin level and blocks denervation‐induced skeletal muscle atrophy in mice [ 62 ]. …

[Early stages of myogenesis as seen through the action of the myf-5 gene].

Skeletal muscles in the vertebrate body are derived from the somites, epithelial spheres of cells which segment from the paraxial mesoderm i … uperfamily of helix-loop-helix transcription factors. Another member of the myogenic factor family, myogenin, is subsequently expressed and leads to muscle cell differentiation with activation of the downstream muscle-specific genes. Dorsalization of the somite and subsequent myogenesis depends on the presence of axial structures and dorsal ectoderm. The W …

Cellular and molecular mechanisms of muscle atrophy.

Skeletal muscle is a plastic organ that is maintained by multiple pathways regulating cell and protein turnover. During muscle atrophy, proteolytic systems are activated, and contractile proteins and organelles are removed, resulting in the shrinkage of muscle fibers. Excessive loss of muscle mass is associated with poor prognosis in several diseases, including myopathies and muscular dystrophies, as well as in systemic disorders such as cancer, diabetes, sepsis and heart failure. Muscle loss also occurs during aging. In this paper, we review the key mechanisms that regulate the turnover of co

Совпадение в полном тексте источника
… These studies demonstrated that myogenin activates the expression of the E3 ubiquitin ligases atrogin-1 and MuRF1, and that myogenin knockout mice fail to upregulate atrogin-1 and MuRF1 following denervation and are protected from atrophy. … A recent study revealed an unexpected connection between TNFα signaling and myogenin on MuRF1 and atrogin-1 expression: TNFα treatment causes upregulation of myogenin, MuRF1 and atrogin-1. …

Дело о амеланотической плазмоцитоидной меланоме с сильным CD138, имитируя многократную миелому - доклад о деле.

Фон и объектив Melanomas имеют различные патологические особенности, которые имитируют другие опухоли, такие, как лимфома, саркома и даже слабо различимая карцинома. Самый недавно выявленный вариант — вариант плазмоцитоида — является необычным вариантом, который может выглядеть как одиночная опухоль или метастатическое заболевание. Из-за его редкости эпидемиологический профиль этого варианта не является достаточно точным. Этот случай иллюстрирует диагностический вызов плазмоцитоидного имитатора клеток, который редко встречается в повседневной практике. 49-year-old Человек подавал многочисленны

Совпадение в полном тексте источника
… Myogenin staining was negative ( Figure 3 ). During hospital admission, laboratory results showed a decreased calcium level (1.35 mmol/L) and normal tumor marker levels (AFP, CEA, PSA, and Ca 19-9). … (F) Cell nuclei show negative staining for myogenin (magnification 40×). Discussion Malignant melanomas may show a variety of cytomorphological characteristics, architectural patterns, and stromal alterations. …

Isolation of internal and external sphincter progenitor cells from the human anal sphincter with or without radiotherapy.

AIM: We aimed to isolate and propagate internal and external anal sphincter progenitor cells from the human anal sphincter, with or without radiotherapy, for tailored cell therapy of faecal incontinence. METHODS: Sphincter progenitor cells were isolated from normal internal and external anal sphincters collected from 10 patients with rectal cancer who had undergone abdominoperineal resection with (n = 6) or without (n = 4) preoperative chemoradiotherapy. The isolated cells and differentiated muscle fibres were identified using immunofluorescence assay, western blotting and reverse transcripti

Melittin, a honeybee venom derived peptide for the treatment of chemotherapy-induced peripheral neuropathy.

Chemotherapy-induced peripheral neuropathy (CIPN) is the most prevalent neurological complication of cancer treatment which involves sensory and motor nerve dysfunction. Severe CIPN has been reported in around 5% of patients treated with single and up to 38% of patients treated with multiple chemotherapeutic agents. Present medications available for CIPN are the use of opioids, nonsteroidal anti-inflammatory agents, and tricyclic antidepressants, which are only marginally effective in treating neuropathic symptoms. In reality, symptom reappears after these drugs are discontinued. The pathogene

Совпадение в полном тексте источника
… Melittin elevates the expression of muscle regeneration factors like myogenin, myoblast determination protein 1 and alpha smooth muscle actin. It suppresses the development of pro-inflammatory cytokines and improves the histological changes of damaged tissue. … Melittin elevates the expression of muscle regeneration factors like myogenin, myoblast determination protein 1 and alpha smooth muscle actin. It suppresses the development of pro-inflammatory cytokines and improves the histological changes of damaged tissue. …

Plasticity and function of human skeletal muscle in relation to disuse and rehabilitation: Influence of ageing and surgery.

In order to study the influence of disuse and aging on skeletal muscle homeostasis, different human models were employed. Effects of chronic … own to promote skeletal muscle hypertrophy and myogenic stem cell proliferation (IGF-Ea, MGF, MyoD, myogenin, HGF). Moreover, an age-specific regulation in myostatin mRNA expression was observed, characterized by an amplified increase in aging skeletal muscle with immobilisation that was followed by less down-regulation during the subsequent phase of re-lo …