Результаты поиска

Quantitative determination of ethyl carbamate in alcoholic beverages using gas chromatography coupled to ion mobility spectrometer.

Ethyl carbamate (EC), a carcinogen in alcoholic beverages, poses a potential hazard to human health. This study developed a gas chromatography-Fourier deconvolution ion mobility spectrometry (GC-FDIMS) method with ammonia dopant for EC quantification. The anhydrous MgSO4 dehydration-assisted dichloromethane extraction of EC in alcoholic beverages simplified the sample pretreatment. The IMS system was established by the FD technique to improve the resolution and sensitivity. The results showed that the limit of detection (LOD) and limit of quantification (LOQ) for EC were 0.34 ng/mL and 1.16 ng

Fluorescent biosensor for lead ion detection based on GR-5 DNAzyme and self-hybridization chain reaction.

BACKGROUND: Lead ion (Pb2+) is a typical heavy metal pollutant, and the water and food contaminated by lead may pose a potential threat to t … tive Pb2+ detection system demonstrates significant potential for a wide range of practical applications in both environment and food monitoring. In addition to its specific application for Pb2+ detection, by introducing different DNAzymes, this SHCR system can be applied for the detection of other heavy metal ions.

DoE based development and optimization of ion sensitive in situ nanoemulgel containing bimatoprost for sustained ocular delivery.

BACKGROUND: The primary treatment for glaucoma involves the chronic instillation (daily) of intraocular pressure (IOP)-lowering eye drops to … ignificant decrease in IOP with the developed formulation (NEG). Other than this, adverse drug reactions (ADR) associated with anti-glaucoma medication, i.e., dry eye and conjunctival hyperemia, were addressed by developing NEG with omega-3 fatty acid and HA. CONCLUSION: This novel formulation offers a competitive option to traditional eye drops becau …

Controlled Magnesium Ion Delivery via Mg-Sputtered Nerve Conduit for Enhancing Peripheral Nerve Regeneration.

Autologous nerve grafting remains the gold standard for treating peripheral nerve injuries; however, it is constrained by limited donor nerve availability, the need for secondary surgeries, and sensory loss at the donor site. Biodegradable material-based nerve conduits have emerged as a promising alternative to address these limitations and enhance nerve regeneration. Among these materials, magnesium stands out due to its exceptional biocompatibility, biofunctionality, and neuroprotective properties. Despite its potential, magnesium's rapid corrosion rate and the need for controlled ion releas…

Effect of the combination of ion-pairs strategies and permeation enhancers on iguratimod transdermal patch against rheumatoid arthritis.

Iguratimod (IGU) is a novel anti-rheumatic drug that has anti-inflammatory effects, inhibits bone destruction, and promotes bone formation. … 720 (backing layer), with component ratios optimized through BBD experiments. The resulting formulations increase the IGU loading and skin penetration of the patch. Then the mechanism of permeation promotion of POCC was investigated in terms of the drug and skin. The molecular interactions between POCC and IGU ion-pairs were investigated by ex vitro ar …

Advanced nitric oxide-activated calcium overload and zinc ion-amplified oxidative imbalance in ferroptosis/apoptosis.

BACKGROUND: Ferroptosis, a programmed cell death driven by lipid peroxidation, relies on an oxidative imbalance. However, tumor cells counteract the effects of oxidative imbalance through adaptive defense mechanisms and metabolic reprogramming, thereby limiting the therapeutic efficacy of ferroptosis. RESULTS: An antitumor strategy was designed to synergistically induce ferroptosis and apoptosis by targeting the release of nitric oxide (NO) and zinc ions (Zn2+), effectively disrupting the redox homeostasis and antioxidant capacity of tumor cells. ZIF-8 degradation in tumor cells triggers Zn2+…

Design and synthesis of a carbohydrate-derived chemosensor for selective Ni(II) ion detection: A turn-off approach.

Nickel, an essential transition metal, plays a vital role in biological systems and industries. However, exposure to nickel can cause severe health issues, such as asthma, dermatitis, pneumonitis, neurological disorders, and cancers of the nasal cavity and lungs. Due to nickel's toxicity and extensive industrial use, efficient sensors for detecting Ni2+ ions in environmental and biological contexts are essential. Carbohydrates, with their inherent water solubility and biocompatibility, are ideal for constructing chemosensors. Incorporating a pyridyl group enhances the selectivity and sensitivi…

Relative biological effectiveness and neural stem cell fate in carbon ion-irradiated human brain organoids.

BACKGROUND AND PURPOSE: Heavy ion therapy offers a promising approach for treating brain tumors due to the high precision of ion beams and biological effectiveness. However, the mechanisms underlying contrast-enhanced brain lesions, which occur more frequently after ions than conventional X-rays, remain to be elucidated. MATERIALS AND METHODS: Cerebral organoids were generated from human embryonic stem cells and exposed to high-energy carbon-ions at early (day 20) and late (day 80) developmental stages. Analyses of gene expression were performed using RT-PCR analysis, and protein expression w…

A Dosimetric Evaluation of the Feasibility and Robustness of Ultra-hypofractionated Carbon Ion Radiotherapy Planning for the Treatment of Prostate Cancer.

BACKGROUND/AIM: This study investigated the dosimetric feasibility and robustness of treatment planning for ultra-hypofractionated carbon ion radiotherapy (CIRT) for prostate cancer, in which the dose to urethra was reduced. PATIENTS AND METHODS: The prescribed dose of 40 Gy (relative biological effectiveness: RBE) was given in 4 fractions using dose constraints based on X-ray stereotactic body radiotherapy studies: urethra Dmax <38 Gy (RBE), bladder V40 <10 cc, and rectum D2cc <38 Gy (RBE). The treatment planning method for ultra-hypofractionated CIRT was developed using the simulation compu…

Prophylactic intestinal resection following carbon-ion radiotherapy for locally recurrent rectal cancer: a case report.

Treatment of locally recurrent rectal cancer (LRRC) after surgery is often complex and challenging. A 52-year-old man received emergency surgery (Hartmann's procedure) for bowel perforation caused by a huge sigmoid colon cancer, followed by treatment for concurrent advanced lower rectal cancer with neoadjuvant chemoradiotherapy and abdominoperineal resection. A solitary lung metastasis emerged afterwards, and was surgically removed. However, the patient developed LRRC in front of the sacrum. As surgical resection for the local recurrence was considered too invasive, carbon-ion radiotherapy (CI

Митохондрийская эндоплазмическая ретикулумная мембрана и ионный обмен кальция: новое направление для старения и нейродегенеративных заболеваний.

Митохондрийские эндоплазмические ретикулумные мембраны служат важнейшими сигнальными узлами, координирующими связь между эндоплазмическим ретикулумом и митохондрией, и играют центральную роль в ионном обмене кальциями. Этот динамический интерфейс регулирует ключевые клеточные процессы, включая биоэнергетический метаболизм, апоптоз, автофагию и реакцию на стресс. Регулирование переноса кальция, связанного с митохондрией эндоплазмической ретикулумной мембраной, может нарушить внутриклеточный гомеостаз, что приводит к дисфункции митохондриала, окислительному стрессу и нейрональной смерти, которые

Совпадение в аннотации оригинала
Mitochondria-associated endoplasmic reticulum membranes serve as crucial signaling hubs mediating communication between the endoplasmic reti … are hallmarks of aging and neurodegenerative diseases. This review systematically examines the functions of protein complexes within mitochondria-associated endoplasmic reticulum membranes and the pathogenic mechanisms of calcium signaling regulated by these membranes in neurodegenerative disorders. It places particular emphasis on structural alteratio …

Wing expansion functional analysis of ion transport peptide gene in Sogatella furcifera (Horváth) (Hemiptera: Delphacidae).

Ion transport peptide (ITP), a superfamily of arthropod neuropeptides, serves a crucial role in regulating various physiological processes such as diuresis, ecdysis behavior, and wing expansion. However, the molecular characteristics, expression profile, and role of ITP in Sogatella furcifera are poorly understood. To elucidate the characteristics and biological function of ITP in S. furcifera, we employed reverse transcription-polymerase chain reaction (RT-PCR) and RNA interference (RNAi) methods. The identified SfITP gene encodes 117 amino acids. The expression of SfITP gradually increased f

Is motherhood still possible after pelvic carbon ion radiotherapy? A promising combined fertility-preservation approach.

INTRODUCTION: Preserving the endocrine and reproductive function in young female cancer patients undergoing pelvic radiation is a significant challenge. While the photon beam radiation's adverse effects on the uterus and ovaries are well established, the impact of pelvic carbon ion radiotherapy on women's reproductive function is largely unexplored. Strategies such as oocyte cryopreservation and ovarian transposition are commonly recommended for safeguarding future fertility. METHODS: This study presents a pioneering case of successful pregnancy after carbon ion radiotherapy for locally advan

Optimal radiation dose to induce an abscopal effect by combining carbon-ion radiotherapy and anti-CTLA4 antibody.

BACKGROUND AND PURPOSE: Although carbon-ion radiotherapy (CIRT) has led to good outcomes, controlling metastasis is still crucial for improving overall survival. This study aimed to evaluate the effectiveness of by two combinations, one of CIRT and anti-CTLA4 antibody, the other of CIRT and anti-PD-1 antibody, applied at different radiation doses for distal tumour and metastasis suppression. MATERIALS AND METHODS: Murine cancer cells (colon carcinoma Colon-26 cells for experiments and osteosarcoma LM8 cells for verification) were grafted into both sides of the hind legs of syngeneic mice. Rig…

Rectal Gas-Induced Dose Changes in Carbon Ion Radiation Therapy for Prostate Cancer: An In Silico Study.

PURPOSE: This study aims to determine dosimetric influence of rectal gas in carbon ion radiation therapy (CIRT) for prostate cancer and to e … This created a dose distribution simulation irradiated with the residual rectal gas. Among 12 fractions (fx) for prostate cancer CIRT, different residual rectal gas fx were used to develop 12 dose distributions, each of which was compared with that in the initial plan. Clinical target volume (Dmin, D99.5%), rectum, and rectal wall (V95%, V80%) paramet …

Evaluation of ER;CR:YSGG Laser and Remineralization Agents on Mineral Density and Ion Levels of Primary and Permanent Enamel.

Objective: The aim of this study is to evaluate the efficacy of two different fluoride varnishes used alone or in combination with laser treatment on permanent and primary tooth enamel. Methods: Ninety-six primary and 96 permanent molar samples were divided into six groups. The levels of calcium, phosphorus, fluoride, and silver ions of each sample were analyzed using energy-dispersive X-ray spectroscopy (EDS). Six different treatments were applied to 12 different groups (n = 15) as control (g1/G1), fluoride varnish (g2/G2), casein phosphopeptide-amorphous calcium phosphate (CPP-ACP)-fluoride …

Non-targeted volatilomics for the authentication of saffron by gas chromatography-ion mobility spectrometry and multivariate curve resolution.

In the present contribution, a novel non-targeted volatilomic study based on headspace GC-IMS (HS-GC-IMS) was developed for the authentication and geographical origin discrimination of saffron. In this regard, multivariate curve resolution-alternating least squares (MCR-ALS) was employed to recover the pure GC elution and IMS profiles of saffron metabolites. Iranian saffron samples from seven important areas were analyzed by HS-GC-IMS. The resulting second-order GC-IMS datasets were organized in a augmented matrix and processed using MCR-ALS with various constraints. The MCR-ALS resolved GC pr

Ion Release and Apatite Formation of Resin Based Pit and Fissure Sealants Containing 45S5 Bioactive Glass.

The purpose of this study was to evaluate a resin based pit and fissure sealant containing 45S5 bioactive glass (BAG) by examining its ion release, pH variation, and apatite-forming properties. To prepare the experimental materials, 45S5 BAG, used as a filler, was incorporated into the light curable resin matrix at concentrations of 0 (control), 12.5, 37.5, and 50.0 wt.%. Ion release, pH variation, and apatite formation (Raman spectrometer and scanning electron microscopy-energy-dispersive X-ray spectrometry measurements) were performed. While no ions were released from the control group, the …

Strong ion gap and anion gap corrected for albumin and lactate in patients with sepsis in the intensive care unit.

INTRODUCTION: Metabolic acidosis is very common amongst critically ill sepsis patients partly due to the presence of unmeasured ions in serum. These ions can be detected by anion gap (AG) or strong ion gap (SIG) concentration values. The purpose of this study is to assess the correlation and potential agreement of the two methods in critically ill patients with sepsis. MATERIALS AND METHODS: The present is a retrospective study including septic patients admitted to the Intensive Care Unit from December 2014 to July 2016. The [SIG] and the [AG] corrected for albumin and lactate ([AGcl]) were c…

Diabetes-induced alteration of metal ion levels declines the activity of MMPs to decrease aortic aneurysm risk.

AIMS: Diabetes mellitus (DM) links the risk of cardiovascular diseases. Inverse to the enhanced expression of matrix metalloproteinases (MMPs), the development of aortic aneurysm is lower in diabetic population. We examined the hypothesis that DM-induced alteration of metal ion levels declines the activity of MMPs to decrease aortic aneurysm risk. METHODS & RESULTS: By culturing vascular smooth muscle cells (VSMCs) or macrophages with different concentrations of glucose in the medium, we confirmed that high glucose significantly increased the expression of fibronectin and CTGF in VSMCs, and i…