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[Paradoxical reaction to antituberculosis treatment in an immunocompetent girl with pulmonary tuberculosis].

Paradoxical reaction to antituberculosis treatment is rare in paediatric population. We report a 9-year-old girl with high fever and productive cough for the last three weeks. Tuberculine test and Quantiferon were positive, erythrocyte sedimentation rate was 64 mm/h, culture and polymerase chain reaction for M. tuberculosis negative, and chest X ray showed a widened right mediastinum. Tuberculosis was diagnosed, therefore treatment with standard doses of rifampicin, isoniazid, pyrazinamide and ethambutol was started. Twenty-one days later she presented high fever with no other symptoms, worsen…

Nuclear miR-320 Mediates Diabetes-Induced Cardiac Dysfunction by Activating Transcription of Fatty Acid Metabolic Genes to Cause Lipotoxicity in the Heart.

RATIONALE: Diabetes mellitus is often associated with cardiovascular complications, which is the leading cause of morbidity and mortality am … profiled the expression of microRNAs (miRNAs) by microarray and quantitative reverse transcription polymerase chain reaction on db/db mice and identified miR-320 as a key miRNA associated with the disease phenotype. We next established the clinical relevance of this finding by showing the upregulation of the same miRNA in the failing heart of patients with diabetes mellit …

Integrated hepatic transcriptome and metabolome reveal the mechanisms of Jiangtang Tiaozhi formula on improving glycolipid metabolic disorder.

BACKGROUND: Glycolipid metabolic disorder includes a series of chronic diseases that are closely associated with disturbances in both glucos … f JTTZF. Liver tissues were analyzed using transcriptomics and metabolomics. Real-time quantitative polymerase chain reaction validated key gene expression. RESULTS: Metabolomics data revealed that JTTZF significantly regulated HFD-induced alterations in glycolipid metabolism, with notable changes in pathways such as the pentose phosphate pathway, steroid hormone biosynth …

Identification and validation of programmed cell death-related biomarkers in severe pneumonia: A diagnostic observational study using bioinformatics analysis.

Severe pneumonia is the leading cause of mortality in individuals with lung disease. Programmed cell death (PCD) is implicated in various pa … s. Expression levels of the biomarkers were further validated by reverse transcription-quantitative polymerase chain reaction. Four biomarkers - BCL2, CDKN2D, DYRK2, and S100A8 - were identified. Functional similarity analysis highlighted strong functional parallels among these biomarkers. Notably, these 4 biomarkers were involved in key processes such as graft-versus-host …

A phase 2 multicenter, prospective, randomized, double-blind study to assess the clinical and antiviral efficacy and safety of nitazoxanide for the treatment of norovirus in hematopoietic stem cell and solid organ transplant recipients.

Norovirus (NoV) may cause or contribute to chronic diarrhea among immunocompromised hosts. Neither vaccines nor antiviral therapies are available to prevent or treat NoV infection. We conducted a National Institute of Health-sponsored multicenter, prospective, randomized, double-blind study of nitazoxanide (NTZ) for the treatment of NoV-infected adult transplant patients between 2018 and 2021. Subjects with a positive stool NoV polymerase chain reaction within 14 days of enrollment and active gastrointestinal symptoms were randomly assigned (1:1) to NTZ or placebo (P) for 56 consecutive doses …

Bioinformatics analysis identifies biomarkers associated with T-cell depletion in systemic lupus erythematosus.

Systemic lupus erythematosus (SLE) is a chronic autoimmune disease that affects multiple organ systems. In SLE, T-cell subsets are closely a … s, molecular regulatory network analysis, molecular docking, and reverse transcription-quantitative polymerase chain reaction validation. IFIT3, IFIH1, CXCL10, and STAT2 were identified as biomarkers. The nomogram demonstrated good predictive performance. These biomarkers were mainly involved in biological processes such as ribosome biogenesis and negative regulation of vi …

Variance expression of MUC17 and mucin histochemistry in the gastrointestinal tract of the desert-adapted Euphrates jerboa (Jaculus euphraticus).

BACKGROUND: Euphrates jerboa is a desert-adapted animal that survives in extreme arid conditions in Iraq and other countries. This might be due to specialized gastrointestinal (GI) adaptations that allow this animal to survive hard conditions. AIM: This study aimed to investigate the morphological, histochemical, and molecular features of the esophagus, stomach, and duodenum. METHODS: Traditional histomorphology, histochemistry, and real-time polymerase chain reaction targeting the membrane-bound mucin (MUC17) gene were conducted. RESULTS: Morphometry revealed a short esophagus, a J-shaped …

NUDT1 aggravates intestinal epithelial barrier injury through oxidative stress in ulcerative colitis.

BACKGROUND: Nudix hydrolase 1 (NUDT1) plays a crucial role in tumours, where it helps limit cellular damage caused by reactive oxygen species. However, the exact function of NUDT1 in ulcerative colitis (UC) is not well understood. METHODS: NUDT1 expression in the intestinal mucosal tissues of patients with UC was analysed using quantitative reverse transcription polymerase chain reaction. A public database was used to analyse the expression of selected signature genes of interest in patients with UC with different NUDT1 expression levels. TH588, a potent NUDT1 inhibitor, was used to treat int…

Differentially expressed circular RNAs and their diagnostic value in children with allergic rhinitis.

BackgroundWe aimed to elucidate the pathogenesis of allergic rhinitis by analyzing aberrantly expressed circular RNAs and exploring their diagnostic value.MethodsBlood samples were collected from allergic rhinitis patients and healthy controls, and circular RNA microarray was performed. The expression of differentially expressed circular RNAs was verified via real-time quantitative reverse transcription polymerase chain reaction, and these RNAs were selected for Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses. Spearman's correlation analysis was used to evaluate the relation…

Re-emergence of Diphtheria in Guinea: An Outbreak Analysis of Vaccination and Disease Control Perspectives.

BACKGROUND: This study presented the key characteristics of patients who tested positive for diphtheria during the outbreak in the Republic of Guinea in 2023 and assessed the influence of some risk factors on disease development. METHODS: The clinical diagnosis of diphtheria was confirmed by detecting diphtheria toxin genes in nasopharyngeal samples collected from suspected patients via 2 reverse transcription-quantitative polymerase chain reaction tests. Bivariate analyses with the χ2 test and the Fisher's exact test were conducted to explore possible associations between diphtheria positivi…

Rare Presentation of Posttransplant Lymphoproliferative Disease in a Pediatric Liver Transplant Recipient: Plasmablastic Lymphoma Complicated With Hemophagocytic Lymphohistiocytosis.

A 3-year-old female patient, who had received a liver transplant from her father 1 year previously to treat biliary atresia, was admitted with fever and pancytopenia. History showed Epstein-Barr virus polymerase chain reaction positivity detected in the patient 3 months earlier; the patient received reduced immunosuppression in doses of tacrolimus, and valganciclovir was administered. Physical exa-mination showed lymphadenopathies at the cervical, axillary and inguinal regions with 2 × 2 cm at diameter, splenomegaly of 5 cm, and fever of 39 °C. Considering the clinical setting of immune suppre…

An SNP-based diagnostic method for Brucella S2 vaccine strain infections.

BACKGROUND: Brucellosis, a zoonotic bacterial infection caused by Brucella species, exhibits a global distribution. The Brucella S2 vaccine strain is known to cause brucellosis. Current serological antibody assays cannot distinguish between infections caused by the S2 strain and those caused by wild-type Brucella. OBJECTIVE: To develop a diagnostic method capable of specifically detecting S2 vaccine strain infections. METHODS: Two probes were designed targeting single nucleotide polymorphism (SNP) loci upstream of the sugar ABC gene; quantitative polymerase chain reaction (qPCR) and droplet …

Seroprevalence of Bovine Brucellosis in Cattle in District Bannu, Khyber Pakhtunkhwa, Pakistan.

Brucellosis is an infectious and highly contagious zoonotic disease caused by species of the Brucella genus, and it holds significant econom … tween infection and various risk factors. All positive samples were tested for pathogens using AMOS polymerase chain reaction. The overall prevalence of brucellosis was 18.75% through the Rose Bengal plate test and 8% by indirect ELISA. AMOS polymerase chain reaction confirmed the presence of Brucella abortus in four ELISA-positive animals. Statistical analysis using the c …

Prevalence, Virulence and Antimicrobial Resistance of Vibrio cholerae in Aquatic Products and Aquaculture Environment in Shanghai.

In this study, we isolated 214 Vibrio cholerae strains from aquatic (shrimp, crab, grass carp, and crucian carp) and their cultured environment in Shanghai, China. The virulence, serotype, and antimicrobial susceptibility were tested, and polymerase chain reaction (PCR) was used to detect antimicrobial resistance genes. Enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC-PCR) was employed for cluster analysis of the isolated strains. The results showed that V. cholerae was found in 47.9% (114/238) of aquatic samples, with the highest detection rate in shrimp (81.1%)…

Assessment of the Initial Acquisition of Streptococcus mutans in Infants Born to Pregnant Women Attending the Lagos University Teaching Hospital.

AIM: Streptococcus mutans has been strongly associated with dental caries. The mother, the primary caregiver in the first year of life is th … es collection from neonates at birth and six weeks, investigated by cultural microbial analysis and polymerase chain reaction to detect S. mutans specific species genes. RESULTS: Total of 24 mother-child pairs assessed. Mothers mean age; 31.29 (+/- 4.6) years. Neonates' mean gestational age; 38.79 (+/-1.4) weeks, with 14 (58.3%) females and 10 (41.7) males. Microbiologica …

Detection of human herpesvirus 7 in conjunctival samples collected from individuals recovering from conjunctivitis.

PURPOSE: Although it is often reported that adenovirus is the most common etiology for infectious conjunctivitis, a recent multi-center clinical study found that adenovirus was confirmed by polymerase chain reaction in only 16% of cases presenting with acute conjunctivitis. Here, we investigated the hypothesis that a member of Herpesviridae could be the underlying etiology in some non-adenoviral cases of conjunctivitis. METHODS: Molecular assays for Herpes Simplex 1 and 2 (HSV-1, HSV-2) and Human Herpesvirus 6A, 6B and 7 (HHV-6A, HHV-6B, HHV-7) were performed on conjunctival samples collected…

Evaluating the Impact of Ultrasonic Irrigation on Bacterial Levels and Activity Following Chemomechanical Procedures.

INTRODUCTION: This single-arm interventional trial aimed to investigate the efficacy of ultrasonic irrigation as a supplementary disinfection approach after chemomechanical procedures (CMP) using molecular techniques based on ribosomal RNA (rRNA) and rRNA genes (referred to as DNA). METHODS: Samples were collected from 35 single-rooted teeth with radiographic evidence of apical periodontitis. Samples were taken after gaining root canal access (S1), (S2), and ultrasonic irrigation (S3). DNA-targeted quantitative polymerase chain reaction using universal primers was used to estimate total bacte…

[The prevalence of mutations underlying development of Helicobacter pylori resistance to antibiotics in Kazan].

BACKGROUND: One of the reasons for the decrease of Helicobacter pylori eradication effectiveness is its resistance to antibiotics. AIM: To … ucosal biopsies was performed using PureLink Genomic DNA Mini Kits (Thermo Fisher Scientific, USA). Polymerase chain reaction was performed using primers specific for the V-region of the 23S gene and the A subunit DNA gyrase encoding gyrA gene region. The sequencing of obtained DNA fragments was performed on 3730 DNA Analyzer. The sequences were searched for point mutation …

Characterization of Acute Myeloid Leukemia With RUNX1/RUNX1T1 Gene Rearrangement: Clinical, Hematological, and Morphological Features.

OBJECTIVES: This study aimed to determine the frequency of RUNX1/RUNX1T1 gene rearrangement in acute myeloid leukemia (AML) patients by polymerase chain reaction (PCR) and analyze their clinical, hematological, and morphological features of positive patients. PATIENTS AND METHODS: A cross-sectional study was conducted in which newly diagnosed patients with AML were included in the study. A total of 101 AML cases were calculated from the World Health Organization (WHO) formula. Sysmex Hematology Analyzer XP-100 (Sysmex Corporation, Kobe, Japan) performed a complete blood picture of these posit…

Молекулярная распространенность и филогеничность вирусов Тика-Борна в мясных и дешевых продуктах в Республике Корея.

В Соединенных Штатах и Европе недавно произошло резкое увеличение числа случаев обнаружения вирусов у животных скота и скотобойни. Хотя в Корее поступили сообщения о случаях заболевания клещами, пищевое загрязнение от вирусов клеща еще не было расследовано. В связи с этим в ходе этого исследования были исследованы случаи распространения в мясных и молочных продуктах острой лихорадки с вирусом тромбоцитопения (SFTSV), вирусом энцефалита, передаваемым клещами, и вирусом геморрагической лихорадки Крыма-Конго (CSFV). В 2021 - 2022 годах на корейском рынке розничной торговли было собрано в общей сл

Совпадение в аннотации оригинала
Tick-borne virus detection in livestock and slaughterhouse animals has recently surged in the United States and Europe. Although cases of pa … llected from a ranch in Anseong-si, Gyeonggi-do, from 2021 to 2022. Real-time reverse transcription-polymerase chain reaction (RT-PCR), nested reverse transcription polymerase chain reaction (RT-nPCR), virus cultivation, and sequence analysis were conducted. SFTSV was detected in 1.53% (3/195) beef and 0.76% (1/130) goat meat samples with a low Ct value titer from 33.18 to …