Результаты поиска

Guggulsterone Induces Apoptosis in Multiple Myeloma Cells by Targeting High Mobility Group Box 1 via Janus Activated Kinase/Signal Transducer and Activator of Transcription Pathway.

Multiple myeloma (MM) is a hematological disorder characterized by the abnormal expansion of plasma cells in the bone marrow. Despite great advances over the past three decades in discovering the efficacious therapies for MM, the disease remains incurable for most patients owing to emergence of drug-resistant cancerous cells. Guggulsterone (GS), a phytosteroid, extracted from the gum resin of guggul plant, has displayed various anticancer activities in vitro and in vivo; however, the molecular mechanisms of its anticancer activity have not been evaluated in MM cells. Therefore, in this study,

Совпадение в полном тексте источника
… After staining, the cells were then analyzed using the BD LSRFortessa cell analyzer (BD Biosciences, Franklin Lakes, NJ, USA). 2.9. … The samples were analyzed by a BD LSRFortessa cell analyzer (BD Biosciences, Franklin Lakes, NJ, USA). 2.14. Statistical Analysis The statistical analysis was performed using GraphPad Prism software version 7.0 (San Diego, CA, USA). …

Бета Адренергическая сигнализация как терапевтическая цель для автономного иммунитета.

Мы недавно определили молекулярный механизм с использованием бета-адренергических рецепторов 1 и 2 (β1/2) при разработке лимфоцитов TH17. Фармакологические и генетические ингибиции этих рецепторов в сочетании, но не отдельно, сказались на способности Т-лимфоцитов производить профламматический интерleукин 17А (IL-17A) и вместо этого способствовали производству защитных клеток Трега, которые скрывают инфлюкин-10 (IL-10). Вместе с тем остается неясным, может ли этот механизм регулирования использоваться в качестве нового терапевтического подхода к аутоиммунным расстройствам при посредничестве Т-л

Совпадение в полном тексте источника
… Cells were incubated at 37°C for 4 hours in RPMI media supplemented with phorbol 12-myristate-13-acetate (PMA; 10 ng/mL), ionomycin (0.5 mg/mL), and BD GolgiPlug Protein Transport Inhibitor (containing brefeldin A; 1 mg/mL; BD Biosciences, Franklin Lakes, NJ, USA). … Cells were assessed using a 4-laser Attune NxT flow cytometer (Thermo Fisher Scientific, Houston, TX, USA), while data was analyzed using FlowJo analysis software (BD Biosciences, Franklin Lakes, NJ, USA). …

B-Cell и T-Cell популяции в периферальной крови, связанные с лечением Ocrelizumab.

СПРАВОЧНАЯ ИНФОРМАЦИЯ/AIM: Ocrelizumab, a CD20-targeting Моноклональные антитела используются для лечения множественных склерозов. Цель этого исследования заключалась в изучении полезности периферийных поднаборов кровеносных клеток для прогнозирования реакции на орлезизумаб при повторном переносе множественных склерозов (RRMS). ПАТИЕНТЫ И МЕТОДЫ: Thirty-one пациенты, у которых IRMS устойчиво к первичным иммуномодуляционным агентам, были зачислены в учебные заведения, и были приняты последующие меры в отношении 12 Лечение при окролизумабском лечении за месяцы. За деятельностью, связанной с забо

Совпадение в полном тексте источника
… The cells were then incubated with fluorescently labeled monoclonal antibodies: anti-human CD25-APC, CD45RA-fluorescein isothiocyanate (FITC), CD49d-phycoerythrin (PE)/Cyanine5, CD197 (CCR7)-PE/Cyanine5, CD19-APC (BD Biosciences, Franklin Lakes, NJ, USA), CD5-PE/Cyanine5, CD183 (CXCR3)-PE/Cyanine5, CD3 … -Alexa Fluor 700, and CD20-APC/Cyanine7 (BD Pharmingen, Franklin Lakes, NJ, USA); CD4-BV785, CD8-BV510, CD27-BV711, CD127-BV421, CD24-BV421, IgD-APC/Cyanine7, CD38-Alexa Fluor 700, CD138-BV510, CD196 (CCR6)-BV711, CD185 (CXCR5)-APC/Cyanine7, and CD279 PD1-BV650 (Biolegend, San Diego, CA, USA) for 20 …

Prognostic Value of PSMB5 and Correlations with LC3II and Reactive Oxygen Species Levels in the Bone Marrow Mononuclear Cells of Bortezomib-Resistant Multiple Myeloma Patients.

Proteasome inhibitors (PIs) constitute the most common type of induction treatment for multiple myeloma. Interactions between the proteasome, autophagy, and reactive oxygen species (ROS) have been shown in the past, thus emphasizing the need for a better understanding of the underlying pathophysiology. For this study, bone marrow mononuclear cells from 110 myeloma patients were collected at different disease stages. PSMB5 and LC3I/II protein levels were determined using Western blot, proteasome proteolytic activity (PPA) with spectrofluorometry, and ROS with flow cytometry. PSMB5 accumulation

Совпадение в полном тексте источника
… , Franklin Lakes, NJ, USA) to evaluate disease aggressiveness, and a mouse Anti-CD34 linked to phycoerythrin-cyanine5 (Anti-CD34PeCy5) (clone 581, BD Biosciences, Franklin Lakes, NJ, USA) as a lymphohematopoietic stem cell marker. … Flow cytometry analysis was performed using the BD FACS Calibur™ cell cytometer (BD Biosciences, Franklin Lakes, NJ, USA), and 400,000 cells were acquired per sample for the analysis. …

Range-wide Yangtze freshwater dolphin expedition: The last chance to see Baiji?

BACKGROUND, AIM AND SCOPE: There are two species of fresh water cetaceans surviving in the Yangtze River system in China: Baiji (Lipotes vex … rvation efforts in the natural reserves, and even in the entire Yangtze River system, including the lakes, should not be ignored or abandoned at any time. The activities contributing to the conservation of the Baiji and the porpoise in the wild have the incidental effect of benefiting the entire Yangtze ecosystem and other rare threatened species. The d …

Dexamethasone co-medication in cancer patients undergoing chemotherapy causes substantial immunomodulatory effects with implications for chemo-immunotherapy strategies.

The glucocorticoid (GC) steroid dexamethasone (Dex) is used as a supportive care co-medication for cancer patients undergoing standard care pemetrexed/platinum doublet chemotherapy. As trials for new cancer immunotherapy treatments increase in prevalence, it is important to track the immunological changes induced by co-medications commonly used in the clinic, but not specifically included in trial design or in pre-clinical models. Here, we document a number of Dex -induced immunological effects, including a large-scale lymphodepletive effect particularly affecting CD4+ T cells but also CD8+ T

The 2026 Bundibugyo Ebola Outbreak: A Warning for Global Preparedness for Future Epidemics.

Dear Editor, The 2026 Bundibugyo Ebolavirus (BDBV) outbreak has once again demonstrated that the threat of emerging diseases remains a major … e that blanket travel restrictions and border closures are impractical. As communities in the Great Lakes region routinely cross national borders for trade and healthcare, coordinated regional surveillance and timely information sharing are likely to be more effective than broad border closures in mitigating disease transmission[8]. Third, the protectio …

Pancreatic Proteolytic Enzymes and Cancer: New Support for an Old Theory.

In 1905, the embryologist John Beard first proposed that pancreatic proteolytic enzymes had potential as a treatment for cancer. His theories were dismissed by the medical world a decade later, but various practitioners have kept the concept alive through the publication of case reports of cancer patients treated with pancreatic proteolytic enzymes. In the last 2 decades, studies of the role of proteases in physiology have made it clear that they do more than digest food. This article reviews the history of the clinical use of pancreatic proteolytic enzymes in cancer treatment, and recent rese

Cardio-Oncology and Multi-Imaging Modalities.

Early detection and the rise of targeted cancer treatment have led to increased overall survival and decreased mortality among cancer patients. As the cancer survivor population ages, there is an increased risk for cardiovascular disease due to pre-existing comorbidities, deconditioning during therapy, or the natural progression of aging. Furthermore, with emerging oncologic therapies, there is an increased recognition of their potential cardiovascular toxicities. Indeed, heart disease is the leading cause of death in cancer survivors, which may reflect upon both the success of novel oncologic

N-Methyl Pyrrolidone (NMP) Alleviates Lipopolysaccharide (LPS)-Induced Inflammatory Injury in Articular Chondrocytes.

BACKGROUND Studies on the chondrocyte inflammatory injury are very important for understanding the pathogenesis and clinical treatment of osteoarthritis (OA). Evidence suggests that N-methyl pyrrolidone (NMP) may be used as an adjuvant therapy alongside established methods of OA treatment. This study investigated the effect of NMP on chondrocyte inflammatory injury and explored the underlying molecular mechanism. MATERIAL AND METHODS To mimic the inflammatory injury in vitro, the articular chondrocyte line ATDC5 was simulated with lipopolysaccharide (LPS). ATDC5 cells were treated with various

Совпадение в полном тексте источника
… Apoptotic assay Apoptotic cells were analyzed using the Annexin V-FITC/PI apoptosis detection kit (BD Biosciences, Franklin Lakes, NJ, USA) per the manufacturer’s instructions. … Flow cytometry (FACSCalibur; Becton-Dickinson, Franklin Lakes, NJ, USA) was used to analyzed the apoptosis of cells. Tests were repeated at least 3 times. …

Toxicity management strategies for next-generation novel therapeutics in multiple myeloma.

The therapeutic options available for patients with multiple myeloma have greatly expanded over the past decade and incorporating these novel agents into routine clinical practice has significantly improved outcomes. The next generation of therapeutics is available for relapsed and refractory patients either as standard of care or in clinical trial, and these drugs represent a generational paradigm shift. Patients now have access to a multitude of novel immunotherapeutics, including monoclonal antibodies, an antibody-drug conjugate, chimeric antigen receptor T-cells (CAR-T), and bispecific T-c

Integration of Patient-Reported Outcome Measures in the Electronic Health Record: The Veterans Affairs Experience.

PurposeThere are growing efforts to integrate patient-reported outcome (PRO) data into electronic health records (EHRs) to bring together disparate sources of patient information and improve medical care. PRO measures can be used to assess cancer symptom presence and severity. Integrating PRO tools in EHRs can alert providers to address symptoms, which is an essential component of comprehensive oncology care.MethodsWe modified a PRO used to measure cancer and end-of-life symptoms, the Edmonton Symptom Assessment System to create the Veteran Symptom Assessment System (VSAS). VSAS was implemente

Lower-Risk Myelodysplastic Syndrome (MDS) Patients Exhibit Diminished Proteasome Proteolytic Activity and High Intracellular Reactive Oxygen Species (ROS) Levels.

Myelodysplastic syndromes (MDS) constitute a heterogeneous group of clonal hematopoietic stem cell disorders characterized by ineffective hematopoiesis and an elevated risk of transformation to acute myeloid leukemia (AML). Available disease-modifying treatment approaches are limited. The ineffectiveness of proteasome inhibitors (PIs) in MDS patients is currently investigated, although it is unclear whether they rapidly develop resistance to PIs or whether proteasome proteolytic activity (PPA) is constitutively lower in the hematopoietic cells of these patients, thus limiting treatment effecti

Совпадение в полном тексте источника
… The samples were analyzed with a FACSCalibur™ cytometer (BD Biosciences, Franklin Lakes, New Jersey, United States). For each sample, 200,000 ungated events were acquired. … The CD34+ population was gated using an anti-CD34 mouse IgG antibody conjugated with Alexa Fluor 647 (Thermo Fisher Scientific, Waltham, Massachusetts, United States), and the data were analyzed using FlowJo (BD Biosciences, Franklin Lakes, New Jersey, United States). …

Progression-Free Survival of a Patient with Advanced Hepatocellular Carcinoma Treated with Adoptive Cell Therapy Using Natural Killer Cells: A Case Report.

Adoptive cell therapy (ACT) is a promising treatment that is considered safe and efficient. Natural killer (NK) cells play an important role in the innate immune system and destroy target cells such as tumor cells without prior sensitization. Here, we report a 59-year-old man with advanced diffuse hepatocellular carcinoma (HCC) who underwent 17 courses of NK cell treatment from March 2017 to July 2018. Although he presented with progressive disease, his hydrothorax and ascites decreased, and his state of mind, appetite and quality of life were markedly improved after treatment versus at admiss

Совпадение в полном тексте источника
… The cells were incubated in the dark for 15 minutes and washed once with PBS, and the cell purity was detected by an Accuri C6 flow cytometer (BD Biosciences, Franklin Lakes, NY, USA). Cell viability was also detected with flow cytometry using FITC-7-amino-actinomycin D (7-AAD) staining. … To assess crucial activation and inhibitory markers, we analyzed the activating receptors CD226 and CD314 (PE-Mouse Anti-Human, CD226; APC-Mouse Anti-Human, CD314; both, Becton, Dickinson and Company, NJ, Franklin Lakes) and the inhibitory receptor CD158a (FITC-Mouse Anti-Human, CD158a, Becton, Dickinson …

The Transcription Axes ERK-Elk1, JNK-cJun, and JAK-STAT Promote Autophagy Activation and Proteasome Inhibitor Resistance in Prostate Cancer Cells.

The rapid emergence of resistance limits the application of proteasome inhibitors against solid tumors, despite their effectiveness in the treatment of hematological malignancies. Resistant phenotypes are complex and multifaceted, and, thus, the mechanisms involved have not been adequately described. In this study, a Bortezomib-resistant prostate cancer cell line is created by using the PC-3 cell as a prostate carcinoma model of high metastatic potential. The main biochemical differences and adaptations exhibited by the resistant cells revolve around apoptosis evasion, autophagy induction (fun

Совпадение в полном тексте источника
… Afterward, they were stained with propidium iodide (PI) and Annexin V-FITC (BD Biosciences, Franklin Lakes, NJ, USA) for 15 min at room temperature in the dark [ 52 ]. … The subsequent analysis was performed with the FlowJo V10 software (BD Biosciences, Franklin Lakes, NJ, USA). 2.5. …

Влияние краткосрочной прогрессивной физической тренировки на активность заболевания у пациентов с подозрительной множественной миеломой и моноклональной гамма-патологией неопределенного значения: односторонний пилотный исследование.

ОБЪЕКТ: Высокий уровень физической активности связан с уменьшением риска развития крови опухоли множественной миеломы (ММ). ММ предшествует асимптоматическим стадиям моноклональной гамма-патологии неопределенного значения (MGUS) и дымящей множественной миеломы (SMM), которые клинически управляются методом наблюдения. Случайный исследователь (N = 1) бывшего высшего спортсмена в возрасте 44 лет показал, что многофакторная программа физических упражнений обратила активность SMM. С учетом результатов этого предыдущего тематического исследования в настоящем экспериментальном исследовании сначала ра

Совпадение в полном тексте источника
… Viability was determined using a fixable viability stain (FVS620, BD Biosciences, Franklin Lakes, NJ, USA). … All antibodies were purchased from BD Biosciences (Franklin Lakes, NJ, USA) with the exception of anti-KLRG1 which was purchased from BioLegend (San Diego, CA, USA). …

Allele-specific vitamin D receptor binding is associated with pediatric-onset multiple sclerosis.

BACKGROUND AND OBJECTIVES: The genetic basis of adult-onset multiple sclerosis (MS) is well-studied, but less is known about pediatric-onset MS (pedMS), comprising approximately 5% of all MS onsets. Mendelian randomization (MR) studies have demonstrated evidence for a causal association between MS and both 25-hydroxyvitamin D [25(OH)D] serum levels and genetic variation related to vitamin D receptor (VDR) binding. The objective was to identify whether VDR binding variants (VDR-BVs) previously implicated in adult-onset MS were associated with pedMS using genetic instrumental variables (GIVs).

Высоконасыщенная, длинноцепная жирная кислотная кетогенная диета негативно влияет на визуальную и моторно-сенсорную функцию в предклинической модели множественного склероза.

ВВЕДЕНИЕ: Множественный склероз (MS) представляет собой нейродегенеративное состояние, приводящее к демилеляции центральной нервной системы. Визуальное расстройство, уменьшение слоя сетчатки нервно-паралитического слоя и нарушение электрической функции в клетках сетчатки на протяжении всего периода развития болезни являются полезными маркерами успеха лечения. Текущие исследования, посвященные изучению воздействия кетогенного рациона (КД) в качестве котерапии, показывают многообещающие антивоспалительные свойства, однако исследования по-прежнему ограничены различиями в экспериментальной структу

Совпадение в полном тексте источника
… Louis, MO, USA) and complete Freund’s adjuvant (Sigma Aldrich) which contained desiccated Mycobacterium tuberculosis H37Ra (200 µg; BD Difco, Franklin Lakes NJ, USA). The mixture was injected subcutaneously at two spots along the back. … Prior to starting, mice were dark-adapted overnight then anesthetized with intraperitoneal injection of ketamine (30 mg/kg, Mylan, Canonsburg, PA, USA), xylazine (5 mg/kg, Akorn Inc., Lake Forest, IL, USA), and acepromazine (2.3 mg/kg, Rattlesnake Drugs, Scottsdale, AZ, USA). …

NAT10 Regulates LPS-Induced Inflammation via Stabilization of N4-Acetylated PTX3 mRNA in Human Dental Pulp Stem Cells.

Severe dental pulp inflammation can lead to tissue lysis and destruction, underscoring the necessity for effective treatment of pulpitis. N-acetyltransferase 10 (NAT10)-mediated N4-acetylcytidine (ac4C) modification has recently emerged as a key regulator in inflammatory processes. However, whether NAT10 affects the inflammatory response in human dental pulp stem cells (hDPSCs) remains unelucidated. In this study, elevated NAT10 expression was observed in pulpitis tissues and LPS-stimulated hDPSCs. Knockdown of NAT10 led to reduced inflammatory gene expression and lower reactive oxygen species

Совпадение в полном тексте источника
… Cellular analysis was performed using a flow cytometer (BD Biosciences, Franklin Lakes, NJ, USA). 4.6. Immunohistochemistry The healthy and inflamed human dental pulp was processed for immunohistochemical analysis. Following dehydration and paraffin embedding, 5 μm sections were prepared. … Flow cytometry (LSRFortessa, Becton Dickinson, Franklin Lakes, NJ, USA) was used to identify labeled cells, and fluorescence emission was analyzed by an inverted fluorescence microscope (Olympus IX83 + DP74, Tokyo, Japan) equipped with a TRITC filter set. 4.14. …

MicroRNA changes with macro potential contribute to secondary immunodeficiency in chronic lymphocytic leukemia during epstein barr virus reactivation.

Chronic lymphocytic leukemia (CLL) is characterized by immune dysfunctions driven by miRNA deregulation and the activation of immune checkpoint pathways, which contribute to disease progression and secondary immunodeficiency (SID). This study examines the interplay between miRNA expression profiles, Epstein-Barr virus (EBV) reactivation, and immune checkpoint pathways in the context of small intestine disease (SID) development in chronic lymphocytic leukemia (CLL). Patients were stratified into groups based on the presence of SID and EBV reactivation. Comprehensive analyses included miRNA prof

Совпадение в полном тексте источника
… To enhance the stability of the antibodies, assessed using a violet laser, BD Horizon™ Brilliant Stain Buffer (BD, Franklin Lakes, NJ, USA) was used. … Erythrocytes were removed with lysis buffer (BD, Franklin Lakes, NJ, USA), and cells were washed with BD Pharmingen™ Stain Buffer (BSA) (BD, Franklin Lakes, NJ, USA). …