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Identification of broad anti-coronavirus chemical agents for repurposing against SARS-CoV-2 and variants of concern.

Endemic human coronaviruses (hCoVs) 229E and OC43 cause respiratory disease with recurrent infections, while severe acute respiratory syndrome (SARS)-CoV-2 spreads across the world with impact on health and societies. Here, we report an image-based multicycle infection procedure with α-coronavirus hCoV-229E-eGFP in an arrayed chemical library screen of 5440 clinical and preclinical compounds. Toxicity counter selection and challenge with the β-coronaviruses OC43 and SARS-CoV-2 in tissue culture and human airway epithelial explant cultures (HAEEC) identified four FDA-approved compounds with ora

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… SARS-CoV-2 Delta (NH-RIVM27142/2021) | RIVM (through EVaG) | N/A Chemicals, Peptides, and Recombinant Proteins Cell culture reagents | / | / DMEM medium | Sigma-Aldrich | Cat #D6429 Non-essential amino acids (NEAA) | Sigma-Aldrich | Cat #M7145 Fetal Calf Serum (FBS) | Gibco | Cat #10270-106 Penicillin-streptomycin …

Эпигенетическая ось Лактата-H4K12la-HDAC1 регулирует переход к микроглиальному состоянию во время экспериментального аутоиммунного энцефаломиелита у женских мышей.

Микроглиальная функциональная пластичность формируется метаболическим и эпигенетическим перепрограммированием, но то, как эти процессы регулируют автоиммунитет центральной нервной системы, остается неясным. Мы находим, что уровень цереброспинарной жидкости лактат коррелирует с множественным склерозом. Используя женские модели мыши экспериментального аутоиммунного энцефаломиэлита, аккумуляция спинного лакта приводит к стойкой микроглиальной гистоне лактилации, связывающей с гликолитической активацией. Микроглиавое исключение лактата дегидрогеназы А уменьшает эту лактиляцию и усугубляет остроту

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… PM150410) with 10% FBS and 1% penicillin–streptomycin. Cells were maintained in an incubator at 37 °C supplemented with 5% CO2. For experimental treatments, HMC3 cells were stimulated with lipopolysaccharide (LPS, 1 μg mL⁻¹) and interferon-gamma (IFN-γ, 100 ng mL⁻¹) to induce an inflammatory state. …

Площадь метилирования ДНК клеток цереброспинальной жидкости в множестве склероза: исследование эпигеномной ассоциации.

Множественный склероз (MS) является хроническим воспалительным заболеванием центральной нервной системы, в которой метилация ДНК может увязывать генетические и экологические факторы риска. Methods: Мы профилировали содержание метилации ДНК по всему геному в клетках цереброспиновой жидкости (CSF) у людей с MS (pwMS) и совпадающими контрольными устройствами. Дифференцированные метилированные позиции (DMP) и регионы (DMR) были интегрированы с транскриптомными данными, аннотациями клеточного хроматина Т и анализом путей. Выражение гамма-протокадерина (ПХДГ) было оценено в первичных подмножествах C

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… For in vitro T cell stimulation, PBMCs were thawed, washed and cultured in R10 medium consisting of RPMI-1640 supplemented with 10% FBS, 1% l -glutamine and 1% penicillin-streptomycin. …

RNA метилтрансферазы METTL8 устанавливает m3C32 в митохондриальном tRNASThr/Ser (UCN) для оптимизации структуры trNA и митохондриального перевода.

Модифицированные нуклеотиды в tRNA являются важными определяющими факторами сворачивания, структуры и функции. Здесь мы идентифицируем METTL8 как митохондриальный белок и активный РНК метилтрансферазы, ответственный за установку m3C32 в митохондриале человека (mt-)tRNAThr и mt-tRNAser (UCN). METTL8 имеет перекрестные связи с антикодоновой стволовой петлей (ASL) многих мт-тРНК в клетках, в связи с чем возникает вопрос о том, каким образом достигается целевая специфичность метилирования. Расчленение элементов распознавания mt-tRNA выявило наличие U34G35 и t6A37/(ms2)i6A37, которые одновременно п

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… Human cell culture HEK293 Flp-In T-Rex cells (ThermoFisher Scientific) were cultured at 37 °C in 5% CO 2 in DMEM (Gibco) supplemented with 10% foetal calf serum (Merck) and 1% penicillin/streptomycin (Gibco) according to standard protocols. …

Coexistence of Autoantibodies against the Golgi Complex and Ro52 Antigen in a Patient with Nonspecific Interstitial Pneumonia.

Nonspecific interstitial pneumonia (NSIP) is often associated with connective tissue diseases (CTD). The diagnosis of NSIP was confirmed in a 63-year-old man by high-resolution computed tomography and an open lung biopsy. Anti-Golgi complex autoantibodies (AGA) and anti-Ro52 antibodies were simultaneously detected at high concentrations. Autoantibodies to aminoacyl-tRNA synthetases (ARS) were negative. The patient was treated with corticosteroids for six months. During the seven-year follow-up, NSIP had a slow progression and patient had not developed the clinical features of CTD. The present

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… Osteitis tuberculosa multiplex cystica: its treatment with streptomycin and promizole. J Pediatr. 1949;35:625–9. Warshauer DM. Splenic sarcoidosis. Semin Ultrasound CT MRI. 2007;28:21–7. Yanardağ H, et al. Bone cysts in sarcoidosis: what is their clinical significance? …

Epidemiology of infections in cancer patients.

Although major advances in the care of cancer patients over the past several decades have resulted in improved survival, infectious complications remain a significant cause of morbidity and mortality. To successfully identify, treat, and prevent infections, a comprehensive understanding of risk factors that predispose to infection and of commonly encountered pathogens is necessary. In addition, clinicians must keep abreast of the changing epidemiology of infections in this population. As therapeutic modalities continue to evolve, as established pathogens become increasingly drug resistant, and

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… The mice were given different oral doses of streptomycin-resistant E. coli for ease of detection, and persistent colonization was determined by the evaluation of fecal flora. …

Therapeutic Modulation of Autophagy in Leukaemia and Lymphoma.

Haematopoiesis is a tightly orchestrated process where a pool of hematopoietic stem and progenitor cells (HSPCs) with high self-renewal potential can give rise to both lymphoid and myeloid lineages. The HSPCs pool is reduced with ageing resulting in few HSPC clones maintaining haematopoiesis thereby reducing blood cell diversity, a phenomenon called clonal haematopoiesis. Clonal expansion of HSPCs carrying specific genetic mutations leads to increased risk for haematological malignancies. Therefore, it comes as no surprise that hematopoietic tumours develop in higher frequency in elderly peopl

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… Elaiophylin, a macrolide antibiotic extracted from Streptomycin melanosporus, is a potent autophagy inhibitor [ 215 ]. In p53 mutant MM cells, Elaiphylin demonstrated an anti-myeloma activity in vitro and in vivo. …

Advanced Technologies for Large Scale Supply of Marine Drugs.

Marine organisms represent a source of unique chemical entities with valuable biomedical potentialities, broad diversity, and complexity. It is essential to ensure a reliable and sustainable supply of marine natural products (MNPs) for their translation into commercial drugs and other valuable products. From a structural point of view and with few exceptions, MNPs of pharmaceutical importance derive from the so-called secondary metabolism of marine organisms. When production strategies rely on marine macroorganisms, harvesting or culturing coupled with extraction procedures frequently remain t

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… It has proven useful for the industrial scale supply of many different antibacterials (Erythromycin, Vancomycin, Streptomycin, penicillins, and cephalosporins), antifungals (Griseofulvin and Amphotericin B), antiparasitics (Avermectins and Fumagillin), and immunosuppressants (Tacrolimus, Cyclosporine …

Engineering with Biomedical Sciences Changing the Horizon of Healthcare-A Review.

In the dynamic realm of healthcare, the convergence of engineering and biomedical sciences has emerged as a pivotal frontier. In this review we go into specific areas of innovation, including medical imaging and diagnosis, developments in biomedical sensors, and drug delivery systems. Wearable biosensors, non-wearable biosensors, and biochips, which include gene chips, protein chips, and cell chips, are all included in the scope of the topic that pertains to biomedical sensors. Extensive research is conducted on drug delivery systems, spanning topics such as the integration of computer modelin

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… Gold nanoparticles (AuNPs) contribute to biosensing by facilitating the detection of various antibiotics through calorimetry, including amoxicillin, chloramphenicol, kanamycin, tetracycline, oxytetracycline, and streptomycin [ 66 ]. …

Anti-Infective-Associated AKI: A Narrative Review of the Epidemiology, Mechanisms, Risk Factors, Biomarkers, Clinical Course, Monitoring, Prevention, and Therapeutic Strategies.

Acute kidney injury (AKI) occurs commonly in hospitalized patients, especially patients in intensive care units (ICUs). Medications are among the major causative factors of AKI. This narrative review addressed and updated different aspects of anti-infective-associated AKI, including amphotericin B, cidofovir, foscarnet, polymyxins, vancomycin, and aminoglycosides. There is no standard definition or operational criteria to describe anti-infective-associated AKI. Characteristically, it usually occurs during the first two weeks of treatment and is typically dose dependent. Functional resolution o

TB AND OTHER CHEST INFECTIONS

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… . • Nevirapine, efavirenz, Ritonavir, Rifampicin, Isoniazid, pyrazinamide are the hepatotoxic drugs • Streptomycin caused SNHL in two patients Conclusion: Improvements in both ATT and ART drugs is needed to shorten the duration of TB treatment, with no drug-drug interactions, and very minimal toxicities …

Abstracts from the 8th Drug Hypersensitivity Meeting (DHM)

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… Piperacillin Labeled 4 0.39 Rifampicin Not labeled 4 0.39 Vancomycin Labeled 4 0.39 Clindamycin Labeled 2 0.19 Fluconazole Labeled 2 0.19 Imipenem Labeled 2 0.19 Metronidazole Labeled 2 0.19 Nevirapine Labeled 2 0.19 Tetracycline Not labeled 1 0.10 Azithromycin Labeled 1 0.10 Floxacillin Not approved 1 0.10 Streptomycin … Vancomycin | Labeled | 4 | 0.39 Clindamycin | Labeled | 2 | 0.19 Fluconazole | Labeled | 2 | 0.19 Imipenem | Labeled | 2 | 0.19 Metronidazole | Labeled | 2 | 0.19 Nevirapine | Labeled | 2 | 0.19 Tetracycline | Not labeled | 1 | 0.10 Azithromycin | Labeled | 1 | 0.10 Floxacillin | Not approved | 1 | 0.10 Streptomycin …

P941 – P1197

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Chest and Mediastinum

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Invited Lectures : Overviews Purinergic signalling: past, present and future.

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Conference Proceedings - 6th International Conference on Molecular Diagnostics and Biomarker Discovery (MDBD 2022): Building Resilience in Biomedical Research : Penang, Malaysia. 11-13 October 2022.

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How histone modifications influence cellular radiosensitivity: Pharmaceutically targeting epigenetic regulators as a promising avenue to overcome radioresistance.

Cancer remains the leading cause of mortality worldwide. Radiotherapy (RT), a cornerstone of oncological treatment for over a century, has achieved great success in various cancers. However, radioresistance remains the primary factor leading to the failure of radiotherapy. Histone modifications in cancer cells are known to play a pivotal role in regulating radiosensitivity by modulating chromatin structure, either by loosening or tightening it. Here, we provide a comprehensive summary of the link between aberrant histone modifications and radiation resistance across various cancer and normal t

Abstracts for NAPCON 2025

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Late Breaking Orals.

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Diseases of the Nervous System

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