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Нейрональный метаболизм в совершенствовании кетогенного рациона в болезненной модели мыши, вызванной бортезомибами.

ВВЕДЕНИЕ: Кетогенная диета (КД) может способствовать целому ряду неврологических заболеваний, включая различные виды периферической нейропатии. Бортезомиб (ББТ) — первая химиотерапия для лечения множественной миэломы, оказывает нейротоксичное воздействие и часто вызывает периферическую нейропатию, вызванную химиотерапией. ЦЕЛИ: Здесь мы проверили, может ли KD предотвратить возникновение небольшой волокнистой нейропатии и поведенческой гиперчувствительности, связанной с периферической нейропатией (BIPN), вызванной бортезомибами. МЕТОДЫ: C57BL/мужчина6 Мышей кормили либо стандартной диетой, либо

Early administration of remdesivir plus convalescent plasma therapy is effective to treat COVID-19 pneumonia in B-cell depleted patients with hematological malignancies.

Patients with hematological malignancies (HMs) are at a higher risk of developing severe form and protracted course of COVID-19 disease. We investigated whether the combination of viral replication inhibition with remdesivir and administration of anti-SARS-CoV-2 immunoglobulins with convalescent plasma (CP) therapy might be sufficient to treat B-cell-depleted patients with COVID-19. We enrolled 20 consecutive patients with various HMs with profound B-cell lymphopenia and COVID-19 pneumonia between December 2020 and May 2021. All patients demonstrated undetectable baseline anti-SARS-CoV-2 immun

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… White blood cells were stained by anti-CD19-PE and anti-CD3-APC (Becton Dickinson, Franklin Lakes, NJ, USA). … In case of anti-CD20 therapy, 500,000 dual-color–labeled leukocytes were acquired on a FASCanto II flow cytometer (Becton Dickinson, Franklin Lakes, NJ, USA), while 100,000 events were analyzed in the follow-up samples. Results were expressed as the percentage of lymphocytes. …

Risk mitigation in divers with persistent (patent) foramen ovale.

In this issue, Anderson and colleagues report follow-up of divers who were found to have a persistent (patent) foramen ovale (PFO) or, in eleven cases, an atrial septal defect (ASD). In most divers diagnosis followed an episode of decompression illness (DCI). The efficacy of closure of the PFO/ASD in preventing future DCI was compared with conservative diving. They reported that in the closure group the occurrence of confirmed DCI decreased significantly compared with pre-closure, but in the conservative group this reduction was not significant. It is believed there are three requirements for

Anti-tumor activities of the new oral pan-RAF inhibitor, TAK-580, used as monotherapy or in combination with novel agents in multiple myeloma.

Many RAS pathway inhibitors, including pan-RAF inhibitors, have shown significant anti-tumor activities in both solid and hematological tumors. The pan-RAF inhibitor, TAK-580, is a representative of the novel RAF inhibitors that act by disrupting RAF homo- or heterodimerization. In this study, we examined the anti-tumor effects of TAK-580 used as monotherapy or in combination with bortezomib, lenalidomide, or other novel agents in multiple myeloma (MM) cells in vitro. TAK-580 monotherapy potently targeted proteins in the RAS-RAF-MEK-ERK signaling pathway and induced potent cytotoxicity and apo

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… CD34-positive cells were then purified again using anti-human CD34 mAbs (Beckman Coulter, Brea, CA, USA), in combination with or without an anti-CD38 antibody (Becton Dickinson, Franklin Lakes, NJ, USA), with a fluorescence activated cell sorting vantage instrument (Becton Dickinson). … Detection of apoptosis with annexin V/propidium iodide (PI) staining Detection of apoptotic cells was done with fluorescein isothiocyanate- or allophycocyanin-labeled annexin V (BD Biosciences, Franklin Lakes, NJ, USA) and PI (Hoffman-La Roche, Basel, Switzerland), as described [ 53 ]. …

Exercise before, during, and after Hospitalization for Allogeneic Hematological Stem Cell Transplant: A Feasibility Randomized Controlled Trial.

People with cancer who undergo allogeneic hematological stem cell transplant (allo-HSCT) experience significant deconditioning that can compromise quality of life. Exercise has shown to be beneficial before or after allo-HSCT; however, little is known about exercise therapy delivered across the continuum of care. We conducted a feasibility randomized controlled trial of exercise delivered prior to admission, during the inpatient stay, and after discharge versus control in people with planned allo-HSCT. Feasibility was assessed via recruitment and retention rates, the incidence of adverse event

ASENT 2022 Annual Meeting.

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Guarding the heart in the era of immunotherapies: insights for cardio-oncology practice.

The advent of cancer immunotherapies, including immune checkpoint inhibitors (ICI), Chimeric antigen receptor (CAR-T) cell therapies, and bispecific antibodies and other immune-activating platforms such as therapeutic vaccines and oncolytic viruses (often used in combination regimens) has revolutionized oncology by improving patient outcomes across diverse malignancies. However, these therapies are increasingly linked to cardiovascular toxicities such as myocarditis, arrhythmias, and heart failure, posing significant clinical challenges. Here we review current evidence on the mechanisms, clini

Идиопатический цервициальный трансверсальный миелит, преобладающий с нижним Лимбом слабая и автономная дисфункция: доклад о реалистическом случае.

Трансверсный миелит (TM) — это редкое нервно-воспалительное расстройство, которое может привести к значительной заболеваемости, если не диагностировать и не лечить. Мы сообщаем о случае 40-летней женщины, которая в течение двух месяцев испытывала постепенную двустороннюю слабость нижних конечностей и недержание мочеиспускания. Неврологическая экспертиза выявила верхние моторные нейроны и определенный уровень сенсорности, что вызывает подозрения в патологии спинного мозга. МРТ шейного отдела позвоночника выявила гиперинтенсивные повреждения, взвешенные на Т2 и Коротком тау инверсионном восстано

Modulation of NCX1 expression in monocytes associates with multiple sclerosis progression.

Ionic imbalance and functional heterogeneity of monocytes play key roles in multiple sclerosis (MS) progression. A better understanding of monocyte response in the context of ionic dysregulation during MS course may have relevant implications for understanding of disease pathogenesis and treatments. The sodium calcium exchanger NCX1 influences monocyte-derived macrophages reactivity under inflammation; however, little is known about its monocyte-specific expression during MS course. By means of RT-PCR, flow cytometry, and confocal analyses, we determined the expression profiling of NCX1 exchan

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… CB16), PE-Cy7 anti-human CD14 (eBioscience, Carlsbad, CA, USA, clone 61D3), rabbit polyclonal anti-NCX1 mAb (Abgent, San Diego, CA), PE anti-FoxP3-all (eBioscience, San Diego, CA, USA, clone PCH101), PE-FoxP3-exon 2 (eBioscience, San Diego, CA, USA, clone 250D/E4), FITC-anti-human Ki-67 (BD, Franklin Lakes … For intracellular detection the fixation/permeabilization solution kit BD Cytofix-Cytoperm (BD Biosciences, Franklin Lakes, NJ, USA) or the fixation and permeabilization FoxP3 buffer kit (eBioscence, San Diego, CA, USA), were employed according to the manufacturer's instructions. …

Mitochondrial Kv1.3 Channels as Target for Treatment of Multiple Myeloma.

Despite several new developments in the treatment of multiple myeloma, all available therapies are only palliative without curative potential and all patients ultimately relapse. Thus, novel therapeutic options are urgently required to prolong survival of or to even cure myeloma. Here, we show that multiple myeloma cells express the potassium channel Kv1.3 in their mitochondria. The mitochondrial Kv1.3 inhibitors PAPTP and PCARBTP are efficient against two tested human multiple myeloma cell lines (L-363 and RPMI-8226) and against ex vivo cultured, patient-derived myeloma cells, while healthy b

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… Membranes were blocked with 4% BSA/PBS for 1 h and incubated with anti-Kv1.3-antibody (Alomone Labs, Jerusalem, Israel, #APC-101) and anti-Tim23-antibody (BD, Franklin Lakes, NJ, USA, #611223). … Flow cytometry data were further analyzed by FlowJo Software version 10.7.2 for Windows (BD, Franklin Lakes, NJ, USA). 3. Results First, we measured Kv1.3 expression in mitochondria in human myeloma cell lines RPMI-8226 and L-363 and, as positive control, in Jurkat cells. …

Respiratory syncytial virus in hematopoietic cell transplant recipients and patients with hematologic malignancies.

In the USA and other western nations, respiratory syncytial virus is one of the most commonly encountered respiratory viruses among patients who have been diagnosed with a hematologic malignancy or who have undergone a stem cell transplant. Multiple studies have been performed to evaluate the complications associated with respiratory syncytial virus infections. Other studies have evaluated therapeutic agents and strategies in which these agents can be used. There have also been numerous reports of outbreaks in bone marrow transplant units and oncology wards, where infection control measures ha

Multiple myeloma in Korea--clinical analysis and treatment results in 61 cases.

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Neisseria meningitidis Induced Fatal Waterhouse-Friderichsen Syndrome in a Patient Presenting With Disseminated Intravascular Coagulation and Multiple Organ Failure.

Neisseria meningitidis-induced acute systemic meningococcal disease is an emergency and a fatal condition that has a high mortality rate. In patients with a fulminant infection, a maculopapular petechial eruption, purpura fulminans, or an ecchymotic lesion are worrisome signs reflecting disseminated intravascular coagulation (DIC) and hint at Waterhouse-Friderichsen syndrome (WFS). Here, we describe a rare case of a patient with a fulminant Neisseria meningitidis-induced acute systemic meningococcal disease presenting with high-grade fever without meningitis symptoms. Fatal septicemia with DIC

Cardiometabolic health in people with HIV: expert consensus review.

ObjectivesTo develop consensus data statements and clinical recommendations to provide guidance for improving cardiometabolic health outcomes in people with HIV based on the knowledge and experience of an international panel of experts.MethodsA targeted literature review including 281 conference presentations, peer-reviewed articles, and background references on cardiometabolic health in adults with HIV published between January 2016 and April 2022 was conducted and used to develop draft consensus data statements. Using a modified Delphi method, an international panel of 16 experts convened in

Prehabilitation Exercise Training to Target Improved Muscle Strength in Pretransplant Patients Diagnosed With Multiple Myeloma: Protocol for a Pilot Randomized Controlled Trial.

BACKGROUND: Muscle mass and strength are severely compromised in patients diagnosed with multiple myeloma, such that the risk of poor overall survival increases as the prevalence of low muscle mass, also known as sarcopenia, increases. Additionally, at the time of autologous stem cell transplant (ASCT), 51% of patients experience low muscle mass and strength, which can prolong hospitalization and lead to increased risk of obesity, insulin resistance, lowered physical function, and poor quality of life. OBJECTIVE: The PROTECT (Prehabilitation Exercise Training in Multiple Myeloma Patients Unde

Management of an oral ingestion of transdermal fentanyl patches: a case report and literature review.

Purpose. Fentanyl is available as a transdermal system for the treatment of chronic pain in opioid-tolerant patients; however, it carries a black box warning due to both the potency of the product and the potential for abuse. In this report, we describe a case of transbuccal and gastrointestinal ingestion of fentanyl patches and the management of such ingestion. Summary. A 32-year-old man was brought to the emergency department (ED) via emergency medical services for toxic ingestion and suicide attempt. The patient chewed and ingested two illegally purchased transdermal fentanyl patches. In th

Metformin Increases Survival in Hypopharyngeal Cancer Patients with Diabetes Mellitus: Retrospective Cohort Study and Cell-Based Analysis.

Hypopharyngeal squamous cell carcinoma (HSCC) is usually diagnosed at an advanced stage, leading to a poor prognosis. Even after improvement of surgical techniques, chemotherapy, and radiation technology, the survival rate of HSCC remains poor. Metformin, which is commonly used for type 2 diabetes mellitus (DM), has been suggested to reduce the risk of various cancer types. However, only a few clinical studies mentioned the relationship between metformin use and HSCC. Hence, the aim of this study was to elucidate the specific effect and mechanism of action of metformin in hypopharyngeal cancer

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… Cell suspensions were filtered and transferred into a flow cytometer (FACScalibur; Becton Dickinson, Franklin Lakes, NJ, USA) for measuring cell fluorescence (PI excitation at 536 nm; emission at 617 nm). … Samples were filtered and analyzed by flow cytometer (Accuri C6 Plus, BD Biosciences, Franklin Lakes, NJ, USA). 4.7. Quantitative Real-Time Reverse Transcription-PCR (qRT-PCR) qRT-PCR was employed to determine the mRNA expression for Cyclin D1 and Bcl-2. …

Fasting-Mimicking Diet Inhibits Autophagy and Synergizes with Chemotherapy to Promote T-Cell-Dependent Leukemia-Free Survival.

Fasting mimicking diets (FMDs) are effective in the treatment of many solid tumors in mouse models, but their effect on hematologic malignancies is poorly understood, particularly in combination with standard therapies. Here we show that cycles of a 3-day FMD given to high-fat-diet-fed mice once a week increased the efficacy of vincristine to improve survival from BCR-ABL B acute lymphoblastic leukemia (ALL). In mice fed a standard diet, FMD cycles in combination with vincristine promoted cancer-free survival. RNA seq and protein assays revealed a vincristine-dependent decrease in the expressi

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… FlowJo 10 (Becton Dickinson, Franklin Lakes, NJ, USA) was used to analyze data and to prepare figures. 2.7. … Analyses were performed with BD FACS diva on LSR II (Becton Dickinson, Franklin Lakes, NJ, USA). 2.8. Western Blotting Total cell lysates were prepared using the RIPA buffer (Thermo Fisher Scientific Cat# 89900) according to the manufacturer’s instructions. …

Cancer in Lahore, Pakistan, 2010-2019: an incidence study.

ObjectivesTo study the cancer incidence rates over 10 years (2010-2019), in Lahore, Pakistan.DesignAn incidence study.SettingThe population-based Punjab Cancer Registry was established in 2005 in Lahore, which is the provincial metropolis of the province of Punjab (five rivers), and is located in the northeast region of Pakistan. The coordinating office of the Registry is located within Shaukat Khanum Memorial Cancer Hospital and Research Center. Both the active and passive forms of data collection are used.ParticipantsResidents of the district of Lahore diagnosed with cancer. The average annu

Disturbing the Redox Balance Using Buthionine Sulfoximine Radiosensitized Somatostatin Receptor-2 Expressing Pre-Clinical Models to Peptide Receptor Radionuclide Therapy with 177Lu-DOTATATE.

Peptide receptor radionuclide therapy with 177Lu-DOTATATE improves the outcome of patients with somatostatin receptor (SSTR)-expressing neuroendocrine tumours. Nevertheless, stable disease has been the main response pattern observed, with some rare complete responses. Lu-177 exerts about two-thirds of its biological effects via the indirect effects of ionizing radiation that generate reactive oxygen species, eventually leading to oxidative damage and cell death. This provides a rationale for targeting the antioxidant defence system in combination with 177Lu-DOTATATE. In the present study, the

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… Samples were then incubated with Annexin-V Binding Buffer (ThermoFisher, Waltham, MA, USA), Annexin V (BD Biosciences, Franklin Lakes, NJ, USA) and 7-AAD (BD Biosciences, Franklin Lakes, NJ, USA) for 15 min at room temperature. …