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CD4-Positive T-Cell Responses to MOG Peptides in MOG Antibody-Associated Disease.

To clarify T-cell responses in myelin oligodendrocyte glycoprotein (MOG) antibody-associated disease (MOGAD), we cultured the peripheral blood mononuclear cells of 24 patients with MOGAD and 20 with aquaporin-4 (AQP4) antibody-positive neuromyelitis optica spectrum disorders (NMOSD), and those of 17 healthy controls (HCs), in the presence of fourteen MOG peptides covering the full-length MOG, five AQP4 peptides, two myelin basic protein peptides, or two proteolipid protein peptides. Then, we measured T-cell activation markers, such as cell surface CD69 and the intracellular production of granu

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… After incubation for 30 min, the PBMCs were washed and resuspended in phosphate-buffered saline supplemented with 0.5% bovine serum albumin and analyzed by FACS Verse (BD Biosciences, Franklin Lakes, NJ, USA), according to the manufacturer’s instructions. …

DKK-1 in prostate cancer diagnosis and follow up.

BackgroundDickoppf-1 (DKK-1) is a negative regulator of bone formation with tumorigenic potential. The up-regulation of DKK-1 is an early event in prostate cancer (PCa) development, thus we investigated its role as a marker in the diagnosis and prognosis of PCa.MethodsWe retrospectively enrolled 159 patients who underwent prostate biopsy, either for elevated PSA or suspect digital rectal examination, between 2003 and 2010. During the biopsy, one serum sample was collected from all patients; PSA and DKK-1 were measured by ELISA technique. Amongst the biopsy of 159 patients 75 were affected by P

Contextualizing results of randomized trials in smoldering myeloma.

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Fatal septic shock and Waterhouse-Friderichsen syndrome caused by serovar B Capnocytophaga canimorsus in an immunocompetent patient. Case report and review.

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… Antibiotic susceptibility testing was performed by Liofilchem® MIC Test Strips on Mueller Hinton agar plates with 5% of sheep blood (Becton Dickinson, Franklin Lakes, NJ, USA) incubated in 5-10% CO 2 atmosphere. …

A modified method for reducing renal injury in zoledronic Acid treatment of hypercalcemia and adverse skeletal events.

AimsIn this paper, we have reported a previously undescribed risk factor of deterioration of renal function in zoledronic acid treatment of skeletal metastasis - high serum calcium level. Based on this consideration, a modified method of treatment of hypercalcemia (HCM) with zoledronic acid is suggested in this paper.Material and methodsBone scan findings of 1090 cancer patients were analyzed, of which 26 had intense renal parenchymal uptake as a result of HCM or bone metastases. Subsequently, a total of 56 bone metastases patients with zoledronic acid treatment were divided into three groups:

Sphingolipid metabolism and hematologic disorders: current understanding and future directions.

Sphingolipids are not only key structural components of cell membranes but also serve as important signaling molecules, and their metabolic homeostasis is essential for the maintenance of cellular functions. In recent years, with the advancement of technologies such as metabolomics, studies have revealed that sphingolipid metabolic abnormalities are extensively involved in the occurrence and development of various hematological disorders, including immune thrombocytopenia, myeloproliferative neoplasms, graft-versus-host disease, hereditary hematological diseases, and hematologic malignancies.

Мак-1 регулирует иммуноусилительное подавление конкретной стадии болезни через нитриковый путь в аутоиммунном заболевании.

Интегрин Мак-1 играет важнейшую роль в развитии множественного склероза (MS); однако лежащий в его основе механизм не в полной мере изучен. Здесь мы разработали мышь с миелоидной мышью Мак-1-дефицит. Используя экспериментальную аутоиммунную энцефаломиэлит (EAE) модель мыши MS, мы сообщаем, что Мак-1 на миелиоидных клетках является ключом к развитию болезней. Наши данные показывают, что миелиоидный Мак-1 значительно повышает интенсивность EAE и затрудняет регрессию заболеваний. Потеря Mac-1 увеличивает количество клеток Gr-1+ в периферийных тканях и CNS и предпочтительно ускоряет переход моноци

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… Mouse BD Fc receptor (FcR) blocker (catalog no. 553142) was bought from BD Biosciences (Franklin Lakes, NJ). NO synthase (NOS) inhibitor (L-NMMA, catalog no. … Louis, MO) and BD Biosciences (Franklin Lakes, NJ), respectively. Pertussis toxin (catalog no. 179A) was purchased from List Labs (Campbell, CA). Mice WT and Mac-1–deficient (Mac-1KO) mice were all in the C57BL/6J background and used at 8 to 13 weeks old. …

Convenient Access to Expert-Reviewed Health Information via an Alexa Voice Assistant Skill for Patients With Multiple Myeloma: Development Study.

BackgroundPatients with multiple myeloma (MM) have high information needs due to the complexity of the disease and variety of treatments. Digital voice assistants provide support in daily life and can be a convenient tool that even older patients can use to access health information. Voice assistants may therefore be useful in providing digital health services to meet the information needs of patients with MM.ObjectiveWe aim to describe and report on the development, content, and functionality of the first Amazon Alexa voice assistant skill for patients with MM in Germany with the goal of empo

Integrating Patient-Centred Research in the Canadian Cancer Trials Group.

The inclusion of patients as partners in research is a key link in the delivery of patient-centred care in healthcare systems. Despite genuine intentions to engage patients in authentic partnerships, efforts can result in tokenism and benefits of engagement are missed. Understanding how patient engagement provides value along the research to patient-care continuum and how to best engage patients as partners are key. This document describes the method taken by the Canadian Cancer Trials Group (CCTG) to implement meaningful patient centricity and engagement and the benefits realized. Originally,

Oncolytic viruses on the cusp of success?: proceedings of the 9th International Conference on Oncolytic Virus Therapeutics

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Advances in drug therapy for systemic lupus erythematosus.

Systemic lupus erythematosus (SLE) is an autoimmune disorder that afflicts 500,000 people in the United States. There has not been a new SLE drug approved in the United States since 1958. However, a guidance document issued by the Food and Drug Administration in 2005 provided a roadmap for investigators which spawned numerous ongoing clinical trials. Among these, Belimumab, a monoclonal antibody to soluble B lymphocyte stimulator, met its primary endpoints in two large trials and will probably obtain FDA approval soon. Other promising agents targeting a variety of mechanisms of action are curr

Разоблачение смешения воспаления-дегенерации в ранних мембранах: предварительные исследования из ферритина, нейрогранина, ТРЕМ2 и клеточного слоя сетчатки.

Множественный склероз (MS) представляет собой сложную взаимосвязь между иммунной воспалением и нейродегенерацией. Последние достижения в области биомаркеров позволили получить новые сведения о молекулярных основах MS, включая ферритин, нейрогранин, триггерный рецептор, выраженный на миелоидных клетках 2 (TREM2) и световую цепь нейрофильтров. ЦЕЛИ: Настоящее экспериментальное исследование направлено на изучение уровней этих биомаркеров в цереброспинной жидкости (CSF) пациентов MS и изучение их связей с параметрами клинической, когнитивной и оптической томографии (OCT).МЕТОДЫ: Это перекрестное э

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… Peripheral blood samples were collected in Vacutainer tubes (BDInc, Franklin Lakes, NJ) without anticoagulant and centrifuged at 3000 rpm for 7 min to separate the serum fraction from the blood clot. …

Bloodstream infections and mortality-related factors in febrile neutropenic cancer patients.

BackgroundWe performed a prospective observational cohort study to evaluate the causative bacteria and to identify risk factors for mortality in febrile neutropenic (FN) patients with blood stream infection (BSI).Material/methodsWe conducted a prospective data collection on all patients with bacteremia or fungemia. The patients were assigned into low-risk and high-risk groups in accordance with the Multinational Association for Supportive Care in Cancer (MASCC) Risk Index.ResultsThroughout the study period, the patients developed 420 FN episodes. Out of 420 episodes, only 90 (21.4%) were found

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… Blood cultures were performed using the BACTEC 9240 (Becton Dickinson, Franklin Lakes, NJ, USA) automated system. Sensitivity to antibiotics was tested by the disk diffusion method on Mueller-Hinton agar according to Clinical and Laboratory Standards Institute (CCLS) procedures. …

Профиль Cytokines связан с серопозитивностью SARS-COV2 у пациентов с множественным склерозом и его стойкостью в течение шести месяцев.

Справочная информация: Пациенты, страдающие множественным склерозом (pwMS), которые проходят курс лечения синдицирующих заболеваний (DMT), могут проявлять изменяющуюся иммунную реакцию на такие инфекции, как SARS-COV-2. Цель этого исследования состояла в том, чтобы охарактеризовать цитокиновые профили, связанные с предыдущей инфекцией SARS-COV-2, и выявить иммунные маркеры, связанные с устойчивостью юмористической реакции в PwMS. Методы: до внедрения вакцины COVID-19 в Испании было принято на работу в общей сложности 90 ПВМС; 46 из них были серопозитивными в силу присутствия IgG, IgM или IgA а

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… The cytokines were measured using the LEGENDplex ™ Human Inflammation Panel 1, with reagents and analysis software from BioLegend (San Diego, CA, USA), and the samples were acquired using a FACS Fortessa cytometer from Becton Dickinson (Franklin Lakes, NJ, USA), as described in the kit protocol. …

FK506-Binding Protein 11 Is a Novel Plasma Cell-Specific Antibody Folding Catalyst with Increased Expression in Idiopathic Pulmonary Fibrosis.

Antibodies are central effectors of the adaptive immune response, widespread used therapeutics, but also potentially disease-causing biomolecules. Antibody folding catalysts in the plasma cell are incompletely defined. Idiopathic pulmonary fibrosis (IPF) is a fatal chronic lung disease with increasingly recognized autoimmune features. We found elevated expression of FK506-binding protein 11 (FKBP11) in IPF lungs where FKBP11 specifically localized to antibody-producing plasma cells. Suggesting a general role in plasma cells, plasma cell-specific FKBP11 expression was equally observed in lympha

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… Plasmablasts (CD19 + CD27 high CD38 high ) and non-plasmablast B cells (CD19 + CD27 low CD38 low ) were bulk sorted using FACSAria Fusion (BD, Franklin Lakes, NJ, USA. … (Naftali Kaminski) served as a consultant to Boehringer Ingelheim, Third Rock, Pliant, Samumed, NuMedii, Theravance, LifeMax, Three Lake Partners, Optikira, Astra Zeneca, RohBar, Veracyte, Augmanity, CSL Behring, Galapagos, Gilead and Thyron over the last 3 years, reports Equity in Pliant and Thyron, …

CK1α/RUNX2 Axis in the Bone Marrow Microenvironment: A Novel Therapeutic Target in Multiple Myeloma.

Multiple myeloma (MM) is a malignant plasma cell (PC) neoplasm, which also displays pathological bone involvement. Clonal expansion of MM cells in the bone marrow causes a perturbation of bone homeostasis that culminates in MM-associated bone disease (MMABD). We previously demonstrated that the S/T kinase CK1α sustains MM cell survival through the activation of AKT and β-catenin signaling. CK1α is a negative regulator of the Wnt/β-catenin cascade, the activation of which promotes osteogenesis by directly stimulating the expression of RUNX2, the master gene regulator of osteoblastogenesis. In t

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… At the end of the co-culture period, cell sorting by FACSARIA IIIu (Becton-Dickinson, Franklin Lakes, NJ, USA) was used to obtain MSC and MM pure populations. … Cell sorting was performed using a FACSARIA IIIu cytometer and FACS Diva software (Becton-Dickinson, Franklin Lakes, NJ, USA) to discriminate GFP-positive MSC-hTERT cells from GFP-negative INA-6 cells in the different MSC-hTERT–INA-6 co-culture models. 2.5. …

Assessment of cyclosporine serum concentrations on the incidence of acute graft versus host disease post hematopoietic stem cell transplantation.

Allogeneic hematopoietic stem cell transplantation (HSCT) is a curative treatment option for hematological disorders. Cyclosporine (CsA) is one of the major immunosuppressive agents for the prophylaxis against graft versus host disease (GvHD). In this retrospective study, we evaluated the effects of CsA serum levels on the incidence of acute GvHD and transplant outcomes. Retrospective study in 103 adult patients received Hematopoitic Stem Cell Transplantation (HSCT) in the Hematology-Oncology, Bone Marrow Transplantation center at Shariati Hospital in Tehran, Iran. All participants received pr

2026 Приложение к резюме Международного конгресса

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Proteasome inhibition paradoxically degrades gain-of-function mutant p53 R273H in NSCLC and could have therapeutic implications.

Lung cancer is the leading cause of cancer mortality. Despite therapeutic advances in recent years, new treatment strategies are needed to improve outcomes of lung cancer patients. Mutant p53 is prevalent in lung cancers and drives several hallmarks of cancer through a gain-of-function oncogenic program, and often predicts a poorer prognosis. The oncogenicity of mutant p53 is related to its stability and accumulation in cells by evading degradation by the proteasome. Therefore, destabilization of mutant p53 has been sought as a therapeutic strategy, but so far without clinical success. In this

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… Cells were analyzed using a flow cytometer BD FACS Canto II (Franklin Lakes, NJ). Statistical analysis Data represented as mean ± SD of at least 3 biological replicates unless indicated otherwise. Unpaired Student’s t -test was used to compare differences between groups. …

Characteristics of post hoc subgroup analyses of oncology clinical trials: a systematic review.

BackgroundSubgroup analyses in clinical trials assess intervention effects on specific patient subgroups, ensuring generalizability. However, they are usually only able to generate hypotheses rather than definitive conclusions. This study examined the prevalence and characteristics of post hoc subgroup analysis in oncology.MethodsWe systematically reviewed published subgroup analyses from 2000 to 2022. We included articles presenting secondary, post hoc, or subgroup analyses of interventional clinical trials in oncology, cancer survivorship, or cancer screening, published separately from the o