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[The characteristics of the accumulation and the possibility of the long-term preservation of the tick-borne encephalitis virus in ixodid ticks].

A method for tick-borne encephalitis virus reproduction was developed in female Ixodes ticks in the period of postlarval development, if they were kept at elevated temperature (23-37 degrees C). It ensures further conservation of the agent in carriers by decreasing tick maintenance temperature to 4 degrees C.

Discriminant analysis: a method of identifying foci of vector-borne diseases.

Identification of foci of vector-borne diseases does not require knowledge of exact abundances of vectors and pathogens; rather, a focus is defined by the presence, or some minimal level of abundance, of a vector and pathogen. Stepwise discriminant analysis (DA) was applied to data on free-ranging adult wood ticks (the vector) and to data on isolations of Colorado tick fever virus from small mammals. Trap stations were grouped on the basis of relative abundance of wood ticks for one set of analyses and on the presence or absence of virus for another set of analyses. Additional data consisted o

Tick infestation and occurrence of Anaplasma phagocytophilum and piroplasms in cattle in the Republic of Serbia.

Ticks transmit important pathogens affecting cattle such as intracellular bacteria of the genus Anaplasma or protozoa of the order Piroplasm … species on animals was Ixodes ricinus, (n = 167), followed by Haemaphysalis punctata (n = 146) and Dermacentor marginatus (n = 122). On the pasture, however, the most common species was H. punctata (n = 41), I. ricinus (n = 37), and D. marginatus (n = 2). Altogether, 4 out of 163 (2.45%) serum samples of cows were positive for A. phagocytophilum-specific ant …

[Rickettsia helvetica: an emerging tick-borne pathogen in Hungary and Europe].

Rickettsia helvetica belonging to spotted fever group rickettsiae was recently detected by polymerase chain reaction followed by sequencing in European sheep ticks (Ixodes ricinus) from Hungary. Current knowledge on these rickettsiae and the clinical and diagnostic aspects of R. helvetica infection is summarized. In acute cases, R. helvetica is generally responsible for flu-like symptoms. Nevertheless, recent data indicate that in chronic cases, these rickettsiae can be responsible for perimyocarditis resulting sudden cardiac death and might play a role in the pathogenesis of aortic valve dise

Novel potential reservoirs for Borrelia sp. and the agent of human granulocytic ehrlichiosis in Colorado.

Previous work demonstrated that Ixodes spinipalpis ticks maintained an enzootic cycle of Borrelia bissettii and the agent of human granulocytic ehrlichiosis (aoHGE) within woodrats (Neotoma mexicana) and deer mice (Peromyscus maniculatus) in northern Colorado (USA). Because I. spinipalpis is the only known vector of B. bissettii and aoHGE in Colorado, this study was designed to determine the reservoir status of other hosts of I. spinipalpis in five distinct ecological zones along the front range and foothills of Colorado. One hundred and twelve rodents of nine species were examined and 11 (10%

Reaction of the host to the tick-bite. II. Distribution of tick borne encephalitis virus in sucking ticks.

No reactive histological changes of the dermis and no cement cone formation were observed at the insertion site of tick mouthparts by feeding of H. inermis larvae. Conversely, cement formation was observed by feeding of H. inermis female. Fully fed D. marginatus larvae on viraemic mice showed bright fluorescence in oesophagus, suboesophageal ganglion, salivary glands, a few gut cells and in lumen of Malpighian tubuli. We consider the penetration of virus from oesophagus to suboesophageal ganglion as the possible route of primary virus infection. Sections through the D. reticulatus nymphs durin

Consequences of in vitro host shift for St. Louis encephalitis virus.

Understanding the potential for host range shifts and expansions of RNA viruses is critical to predicting the evolutionary and epidemiologic … ution of St. Louis encephalitis virus (SLEV; family Flaviviridae, genus Flavivirus) in vitro in the Dermacentor andersoni line of tick cells to model adaptation to a novel invertebrate host. Our results demonstrated that levels of adaptation and costs in alternate hosts are highly variable among lineages, but also that significant fitness increases in tick ce …

Detection of rickettsial DNA in ticks and wild boars in Kyoto City, Japan.

The tick is a well-known vector for arthropod-borne pathogens, such as tick-borne encephalitis, Lyme disease, Japanese spotted fever and sev … 5 tick species, Haemaphysalis (H.) longicornis, H. flava, H. kitaokai, Amblyomma testudinarium and Dermacentor taiwanensis. Interestingly, the proportion of tick species varied according to geographical region within the city. The ticks collected in the city were reported as potential vectors of pathogens, such as rickettsiosis. We detected rickettsial DNA b …

Rickettsial infection in domestic mammals and their ectoparasites in El Valle de Antón, Coclé, Panamá.

The present research evaluated the presence of Rickettsia spp. on ectoparasites of horses and dogs (using PCR techniques), and their sera (using immunofluorescence assay) in El Valle de Antón town in Panama. A total of 20 horses and 20 dogs were sampled, finding four species of ectoparasites on dogs (the ticks Rhipicephalus sanguineus, Amblyomma ovale, Amblyomma oblongoguttatum, and the flea Ctenocephalides felis), and two tick species on horses (Amblyomma cajennense and Dermacentor nitens). DNA of Rickettsia amblyommii was found in pools of A. cajennense, D. nitens, and R. sanguineus, while R…

Babesia canis canis in dogs from Hungary: detection by PCR and sequencing.

Canine babesiosis in Hungary has always been a severe and frequent disease, attributed to infection with Babesia canis transmitted by Dermacentor reticulatus. Identification of the disease agent has been based merely on size and morphology of the intraerythrocytic parasites and no evidence has been found concerning the subspecies (genotype) of B. canis. Therefore, a molecular survey on natural Babesia infection of dogs in Hungary using PCR and sequence analysis was attempted to clarify the subspecies (genotype) and to obtain information on the occurrence of B. canis. A total of 44 blood sample…

Phylogenetic analysis of spotted fever group Rickettsiae isolated from ticks in Japan.

Eight spotted fever group (SFG) rickettsiae isolated from ticks in Japan were classified by phylogenetic analysis based on the nucleotide sequences of both the citrate synthase-encoding gene (gltA) and 190-kDa antigen-encoding gene (rOmpA). In the phylogenetic tree of gltA, strains DT-1 and FLA-1 isolated from the Dermacentor taiwanensis and Haemaphysalis frava ticks, respectively, were placed as Rickettsia japonica, and strains IO-1, IO-2, IO-25, IM-1 and IP-2 from genus Ixodes ticks were placed as Rickettsia helvetica. Strain AT-1 isolated from the Amblyomma testudinarium belonged to the clu…

Amblyomma crassum Robinson, 1926 (Parasitiformes: Ixodidae): morphological and molecular confirmation of females and nymphs, with description of the nymphal stage.

Ticks (Parasitiformes: Ixodida) comprise about 1008 species worldwide, distributed among the families Argasidae, Ixodidae, and Nuttalliellidae. In the Neotropics, the family Ixodidae represents approximately 14% of global diversity and includes genera of medical and veterinary importance such as Amblyomma, Dermacentor, Haemaphysalis, Ixodes, and Rhipicephalus. However, the taxonomic delimitation and distribution of several neotropical species remain uncertain due to the existence of cryptic species complexes, unreliable historical records, and the lack of consistent morphological descriptions …

First molecular detection of Babesia caballi in camel associated ticks in Uzbekistan, with no evidence of Anaplasma or Rickettsia.

Dromedary camels (Camelus dromedarius) have an important role in food and textile production in arid and semi-arid regions of the world, yet … ll, 32.6% of camels harbored ticks, with 993 specimens collected, belonging to the genera Hyalomma, Dermacentor, and Rhipicephalus, comprising at least six species. Molecular analysis revealed the presence of Babesia caballi DNA in two tick pools, marking the first report of this equine pathogen in Hyalomma ticks from camels in Uzbekistan. No evidence of othe …

Genomic Characterization of a Novel Yezo Virus Revealed in Ixodes pavlovskyi Tick Virome in Western Siberia.

Ixodid ticks are blood-sucking ectoparasites of vertebrates. They constitute an integral part of natural foci and are responsible for the worldwide transmission of infections to humans, which can result in severe symptoms. For instance, the Tomsk region, where three abundant tick species (Dermacentor reticulatus, Ixodes pavlovskyi, I. persulcatus) occur, is an endemic area for tick-borne encephalitis virus (TBEV). An increasing number of novel infectious agents carried by ticks have been identified using metagenomic sequencing. A notable example is the Yezo virus (Orthonairovirus yezoense, YEZ…

Ticks and Tick-Borne Diseases of Livestock in the Middle East and North Africa: A Review.

Ticks are important vectors of an array of viral, bacterial and protozoan pathogens resulting in a wide range of animal and human diseases. … cies infesting livestock representing the following eight genera: Ornithodoros, Otobius, Amblyomma, Dermacentor, Haemaphysalis, Hyalomma, Ixodes, and Rhipicephalus. In addition, 15 pathogens were recorded causing diseases of significance, with Crimean-Congo hemorrhagic fever, theileriosis, babesiosis and anaplasmosis being widely distributed diseases in the r …

Establishment of Amblyomma americanum populations and new records of Borrelia burgdorferi-infected Ixodes scapularis in South Dakota.

Tick-borne diseases are an emerging public health threat in the United States, but surveillance is lacking in some regions. To advance current knowledge of the ecology of ticks and tick-borne diseases in South Dakota, we conducted a survey in the summer of 2019, focusing on the eastern counties of the state. We collected and identified 266 ticks and a subset were tested for the presence of Borrelia burgdorferi by polymerase chain reaction (PCR). Dermacentor variabilis, a ubiquitous species in the state, was the most commonly identified tick, present in all counties surveyed. However, we also i…

Молекулярная идентификация и оценка генетического разнообразия коксиэллоподобных эндосимбионтов, переносимого клещами скота в Алжире.

Напоминающие коксиеллу бактерии представляют собой большую группу еще до изолата и характеризуют бактерии филогенетически близко к агенту Q лихорадки, Coxiella signetii, и часто ассоциируются с иксоидными клещами по всему миру. Цель этого исследования состояла в том, чтобы оценить присутствие эндосимбионтов, подобных коксиелле, в клещах и описать их генетическое разнообразие в различных видах клещей, поражающих скот в Алжире. Из трех мест было собрано в общей сложности 765 клещей. При отборе 20% отобранных клещей (147/765) было выявлено, что в 51,7% (76/147). Секвенирование частичных 16S rRNA

Совпадение в аннотации оригинала
Coxiella-like bacteria are a large group of yet-to-isolate and characterize bacteria phylogenetically close to the agent of Q fever, Coxiell … howed the positions of identified Coxiella bacteria. Eleven of the 13 sequences from Rhipicephalus, Dermacentor and Hyalomma ticks were grouped in a distinct clade, the other two each represent an independent clade. This study reported that CLE are prevalent in cattle ticks. Most of the identified Coxiella-like bacteria, from different species of ticks found …

Diversity, ecology, and seasonality of hard ticks (Acari: Ixodidae) in eastern Croatia.

The diversity of hard tick fauna was studied in different forest communities in 48 localities of eastern Croatia using the dragging-flagging method and by hand from ten different vertebrate hosts. A total of 2,225 specimens comprising seven species in three genera were identified. Ixodes ricinus, the most abundant species (72.8% of all collected specimens) was recorded in 44 localities, followed by Dermacentor reticulatus in 19 localities, while the other five species (D. marginatus, H. concinna, H. inermis, I. canisuga, and I. hexagonus) were recorded in fewer localities. The highest ratio (6…

Detection of Rickettsiae, Borreliae, and Ehrlichiae in Ticks Collected from Walker County, Texas, 2017-2018.

Cases of tick-borne diseases, including spotted fever rickettsioses, borreliosis, babesiosis, anaplasmosis and ehrlichiosis, in the United S … ty of ticks collected were Amblyomma americanum (96.69%) followed by three additional tick species: Dermacentor variabilis (2.59%), Ixodes scapularis (0.52%), and A. maculatum (0.21%). Ticks were pooled and tested for molecular evidence of bacterial and viral pathogens, respectively. All of the 68 pools of A. americanum had molecular evidence of the spotted f …