Результаты поиска

Studies on the species, amount and base composition of nucleic acids in psoriatic scales and normal horny layer.

The amount of RNA as well as DNA is considerably increased in psoriatic scales as compared with normal horny layer; RNA was characterized as a low-molecular tRNA; as concerns the base composition of DNA, no particularities in comparison with other DNA species were found, and the base composition of tRNA revealed that cytosine and guanosine are in excess; pseudouridine could not be detected in psoriatic tRNA.

An AND-Logic Gate-Based Biosensor for Simultaneous Detection of SARS-CoV-2 Nucleic Acids and Nucleocapsid Proteins.

Nucleic acids and proteins are recognized as gold standard biomarkers for disease diagnosis and pathogen detection. However, conventional single-analyte detection methods remain susceptible to false positives caused by manual operational errors or sample contamination, thereby undermining diagnostic reliability and increasing the burden on healthcare systems. To address this limitation, we developed a one-pot isothermal amplification and CRISPR-Cas cooperative system (OIACS) that functions as an AND-logic gate biosensor for the simultaneous detection of SARS-CoV-2 RNA and nucleocapsid protein.…

Salting Out on a Surface: A Versatile Method for Conjugating Carbon Dots With Proteins, Nucleic Acids, and Polysaccharides.

Carbon quantum dots (CQD) constitute a unique class of materials for biomedical applications due to their inherent optical properties and biocompatibility. However, currently, there is no generalized universal procedure for conjugation of CQD with biopolymers, which significantly reduces their practical value. Here we show an ultrafast "salting out on a surface" (SOS) method for the conjugation of CQD with a wide range of biomacromolecules, including proteins, nucleic acids, and polysaccharides. This method relies on the salting out process, which forms a biopolymer layer on the CQD surface th…

Simultaneous detection of SARS-CoV-2 proteins and nucleic acids by coupling PNIPAM with circle-to-circle amplification.

In the past few years, the global uncontrollable spread of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has underscored the need for rapid, sensitive, and accurate detection methods. Given the continuous mutation characteristics of viruses, single-target detection carries the risk of false-positive or false-negative results, whereas dual-target detection can effectively mitigate such risks. Therefore, thermosensitive polymer poly(N-isopropylacrylamide) (PNIPAM) was integrated with an isothermal amplification method-circle-to-circle amplification (C2CA) in this study, enabling i

An anti-fouling multifunctional interface enables hierarchical validation and ultrasensitive electrochemical detection of Coxsackievirus A6 specific nucleic acids.

Coxsackievirus A6 (CVA6) has emerged as the dominant etiological agent of hand, foot and mouth disease worldwide. At present, neither specific antivirals nor effective vaccines are available, creating an urgent need for rapid, low-cost in vitro diagnostics that can interrupt transmission. This study developed an electrochemical biosensor based on a "screening-validation" strategy. It utilizes a MoS2/MWCNT/AgNPs three-dimensional highly conductive network as the substrate, tightly anchored with p(NIPAm-co-SBMA) microgel to form a "protective-conductive" synergistic interface. The capture probe

Rapid and comprehensive detection of viral antibodies and nucleic acids via an acoustofluidic integrated molecular diagnostics chip: AIMDx.

Precise and rapid disease detection is critical for controlling infectious diseases like COVID-19. Current technologies struggle to simultaneously identify viral RNAs and host immune antibodies due to limited integration of sample preparation and detection. Here, we present acoustofluidic integrated molecular diagnostics (AIMDx) on a chip, a platform enabling high-speed, sensitive detection of viral immunoglobulins [immunoglobulin A (IgA), IgG, and IgM] and nucleic acids. AIMDx uses acoustic vortexes and Gor'kov potential wells at a 1/10,000 subwavelength scale for concurrent isolation of viru…

Streamlined extraction of nucleic acids and metabolites from low- and high-biomass samples using isopropanol and matrix tubes.

UNLABELLED: An essential aspect of population-based research is collecting samples outside of a clinical setting. This is crucial because microbial populations are highly dynamic, varying significantly across hosts, environments, and time points, a variability that clinical sample collection alone cannot fully capture. At-home sample collection enables the inclusion of a larger and more diverse group of participants, accounting for differences in ethnicity, age, and other factors. However, managing large studies is challenging due to the complexities involved in sample acquisition, processing,

Разблокирование нейрозащитного потенциала пептидных нуклеиновых кислот 5 (PNA5) в неврологических болезнях: молекулярные механизмы терапевтических подходов.

Пептидные нуклеиновые кислоты (ПНК) представляют собой синтетические нуклеиновые кислотные аналоги, которые дают различные структурные и функциональные преимущества по сравнению с обычной РНК и ДНК, позиционируя их как мощные молекулы в молекулярной биологии. В последнее время ПНА привлекли значительное внимание к их потенциалу в области профилактики и лечения неврологических заболеваний, включая болезнь Альцгеймера (AD), болезнь Паркинсона (PD), множественный склероз (MS), болезнь Хантингтона (HD), амиотропический боковой склероз (ALS), инсульт, травма головного мозга (TBI), травму спинного м

Совпадение в аннотации оригинала
Peptide nucleic acids (PNAs) are synthetic nucleic acid analogues offering distinct structural and functional advantages over conventional RNA and DNA, positioning them as powerful molecules in molecular biology. Recently, PNAs have gained significant attention for their potential in the prevention and management of neurological diseases, including Alzheimer…

Self-Assembly of Ultrasmall 3D Architectures of (l)-Acyclic Threoninol Nucleic Acids with High Thermal and Serum Stability.

The primary challenge of implementing DNA nanostructures in biomedical applications lies in their vulnerability to nuclease degradation and variations in ionic strength. Furthermore, the size minimization of DNA and RNA nanostructures is limited by the stability of the DNA and RNA duplexes. This study presents a solution to these problems through the use of acyclic (l)-threoninol nucleic acid (aTNA), an artificial acyclic nucleic acid, which offers enhanced resilience under physiological conditions. The high stability of homo aTNA duplexes enables the design of durable nanostructures with dime

Efficient Delivery of siRNA via Tetrahedral Framework Nucleic Acids: Inflammation Attenuation and Matrix Regeneration in Temporomandibular Joint Osteoarthritis.

Temporomandibular joint osteoarthritis (TMJOA) is the most common and severe subtype of temporomandibular disease characterized by inflammation and cartilage matrix degradation. Compared with traditional conservative treatment, small interfering RNAs (siRNAs) have emerged as a more efficient gene-targeted therapeutic tool for TMJOA treatment. Nuclear factor kappaB (NF-κB) is a transcription factor orchestrating the inflammatory processes in the pathogenesis of TMJOA. Employing siRNA-NF-κB could theoretically control the development of TMJOA. However, the clinical applications of siRNA-NF-κB ar

Sensitive detection of microRNAs using polyadenine-mediated fluorescent spherical nucleic acids and a microfluidic electrokinetic signal amplification chip.

The identification of tumor-related microRNAs (miRNAs) exhibits excellent promise for the early diagnosis of cancer and other bioanalytical applications. Therefore, we developed a sensitive and efficient biosensor using polyadenine (polyA)-mediated fluorescent spherical nucleic acid (FSNA) for miRNA analysis based on strand displacement reactions on gold nanoparticle (AuNP) surfaces and electrokinetic signal amplification (ESA) on a microfluidic chip. In this FSNA, polyA-DNA biosensor was anchored on AuNP surfaces via intrinsic affinity between adenine and Au. The upright conformational polyA-

Novel Anti Double-Stranded Nucleic Acids Full-Length Recombinant Camelid Heavy-Chain Antibody for the Detection of miRNA.

The discovery that certain diseases have specific miRNA signatures which correspond to disease progression opens a new biomarker category. The detection of these small non-coding RNAs is performed routinely using body fluids or tissues with real-time PCR, next-generation sequencing, or amplification-based miRNA assays. Antibody-based detection systems allow an easy onset handling compared to PCR or sequencing and can be considered as alternative methods to support miRNA diagnostic in the future. In this study, we describe the generation of a camelid heavy-chain-only antibody specifically recog

Dengue and Zika virus capsid proteins bind to membranes and self-assemble into liquid droplets with nucleic acids.

Dengue virus (DENV) and Zika virus (ZIKV) capsid proteins efficiently recruit and surround the viral RNA at the endoplasmic reticulum (ER) membrane to yield nascent viral particles. However, little is known either about the molecular mechanisms by which multiple copies of capsid proteins assemble into nucleocapsids (NCs) or how the NC is recruited and wrapped by the ER membrane during particle morphogenesis. Here, we measured relevant interactions concerning this viral process using purified DENV and ZIKV capsid proteins, membranes mimicking the ER lipid composition, and nucleic acids in in vi…

Anti-Niemann Pick C1 Single-Stranded Oligonucleotides with Locked Nucleic Acids Potently Reduce Ebola Virus Infection In Vitro.

Ebola virus is the causative agent of Ebola virus disease, a severe, often fatal illness in humans. So far, there are no US Food and Drug Administration (FDA)-approved therapeutics directed against Ebola virus. Here, we selected the host factor Niemann-Pick C1 (NPC1), which has been shown to be essential for Ebola virus entry into host cytoplasm, as a therapeutic target for suppression by locked nucleic acid-modified antisense oligonucleotides. Screening of antisense oligonucleotides in human and murine cell lines led to identification of candidates with up to 94% knockdown efficiency and 50%

Bivalent Display of Dicysteine on Peptide Nucleic Acids for Homogenous DNA/RNA Detection through in Situ Fluorescence Labelling.

Fluorogenic probes that signal the presence of specific DNA or RNA sequences are key enabling tools for molecular disease diagnosis and imaging studies. Usually, at least one fluorophore is attached through covalent bonding to an oligonucleotide probe. However, the additional conjugation step increases costs. Here we introduce a method that avoids the requirement for the preparation of fluorescence-labelled oligonucleotides and provides the opportunity to alter the fluorogenic reporter dye without resynthesis. The method is based on adjacent hybridization of two dicysteine-containing peptide n

A novel paramagnetic relaxation enhancement tag for nucleic acids: a tool to study structure and dynamics of RNA.

In this work, we present a novel 2,2,6,6-tetramethylpiperidine 1-oxyl (TEMPO) radical phosphoramidite building block, which can be attached to the 5'-terminus of nucleic acids. To investigate the paramagnetic relaxation enhancement (PRE) emanating from this radical center, we incorporated the TEMPO label into various types of RNAs. We measured proton PREs for selectively (13)C-isotope labeled nucleotides to derive long-range distance restraints in a short 15 nucleotide stem-loop model system, underscoring the potential of the 5'-TEMPO tag to determine long-range distance restraints for solutio…