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Short- and Long-Term Outcomes of Hematologic Malignancy Patients After Cardiopulmonary Resuscitation: Experience of a Large Oncology Center.

PurposeThe objective of this study is to describe characteristics and short- and long-term outcomes of patients with hematologic malignancies who received cardiopulmonary resuscitation (CPR).MethodsA retrospective review was conducted of all Code Blues at a large comprehensive cancer center. Demographic, clinical, and outcome variables were analyzed for patients with a hematologic malignancy who underwent CPR.ResultsOf 258 patients, 60.1% had leukemia. Outcomes included return of spontaneous circulation (70.2%), hospital survival (12%), and 90-day, 6-month, and 1-year survival rates of 9.8%, 8

Isolation of a Highly Purified HSC-enriched CD34+CD90+CD45RA- Cell Subset for Allogeneic Transplantation in the Nonhuman Primate Large-animal Model.

Allogeneic hematopoietic stem cell transplantation (allo-HCT) is a common treatment for patients suffering from different hematological disorders. Allo-HCT in combination with hematopoietic stem cell (HSC) gene therapy is considered a promising treatment option for millions of patients with HIV+ and acute myeloid leukemia. Most currently available HSC gene therapy approaches target CD34-enriched cell fractions, a heterogeneous mix of mostly progenitor cells and only very few HSCs with long-term multilineage engraftment potential. As a consequence, gene therapy approaches are currently limited

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… MATERIALS AND METHODS Flow Cytometry Analysis and Fluorescence-activated Cell Sorter Antibodies used for flow-cytometric analysis and fluorescence-activated cell sorting (FACS) of rhesus macaque cells include anti-CD34 (clone 563, BD, Franklin Lakes, NJ), anti-CD45 (clone D058-1283, BD), anti-CD45RA …

N-Glycosylation Alteration of Serum and Salivary Immunoglobulin a Is a Possible Biomarker in Oral Mucositis.

BackgroundOral and enteral mucositis due to high-dose cytostatic treatment administered during autologous and allogeneic stem-cell transplantation increases mortality. Salivary secretory immunoglobulin A (sIgA) is a basic pillar of local immunity in the first line of defense. Altered salivary sialoglycoprotein carbohydrates are important in the pathologies in the oral cavity including inflammation, infection and neoplasia. Therefore, we assessed whether changes in the salivary and serum IgA glycosylation correlated with development and severity of oral mucositis.MethodsUsing capillary electrop

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… Serum samples were collected using clot activator containing serum tubes (BD Biosciences, Franklin Lakes, NJ, USA). The collected blood samples were centrifuged at 7500× g for 30 min and the serum fractions were stored at −70 °C one hour after collection until further processing. 2.3. …

Sclerostin induced tumor growth, bone metastasis and osteolysis in breast cancer.

Breast cancer is the second leading cause of cancer-related deaths among women worldwide. Many patients suffer from bone metastasis. Sclerostin, a key regulator of normal bone remodeling, is critically involved in osteolytic bone diseases. However, its role in breast cancer bone metastasis remains unknown. Here, we found that sclerostin was overexpressed in breast cancer tumor tissues and cell lines. Inhibition of sclerostin by antibody (Scl-Ab) significantly reduced migration and invasion of MDA-MB-231 and MCF-7 cells in a time- and dose-dependent manner. In xenograft model, sclerostin inhibi

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… Invasion Assay The invasive capacity of cancer cells was confirmed in vitro using a Transwell chamber coated with Matrigel® (Becton Dickinson, Franklin Lakes, NJ), in accordance with the manufacturer’s recommendation. Sci-Ab (0, 1 or 4 µg/ml) was added to MDA-MB-231 and MCF-7 cells for 24 hours. …

A phase I dose-escalation study to assess safety, tolerability, pharmacokinetics, and preliminary efficacy of the dual mTORC1/mTORC2 kinase inhibitor CC-223 in patients with advanced solid tumors or multiple myeloma.

BackgroundThe mammalian target of rapamycin (mTOR) pathway is essential for tumor development, yet mTOR inhibitors have yielded modest results. This phase 1 study investigated the mTORC1/mTORC2 inhibitor CC-223 in patients with advanced cancer.MethodsPatients with advanced solid tumors or multiple myeloma received an initial dose of 7.5-60 mg of CC-223, followed by oral daily dosing in 28-day cycles until disease progression. The primary objective was to determine the safety, tolerability, nontolerated dosage, maximum tolerated dosage (MTD), and preliminary pharmacokinetic profile. Secondary o

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… The samples were treated with Lyse/Fix Buffer (Becton‐Dickinson; Franklin Lakes, NJ) for 10 minutes at 37°C. The plates were centrifuged and aspirated, and 1 mL of ice‐cold 100% methanol was added to the wells. …

Сочетание виртуальной реальности и гипноза? Исследование опыта работы с пациентами с множественной миеломой после трансплантации стволовых клеток.

Множественная миелома (ММ) и трансплантация стволовых клеток (ЦТ) оказывают значительное влияние на качество жизни пациентов. Виртуальная реальность с гипнозом (ОВГ) стала многообещающим нефармакологическим вмешательством для решения этих проблем, однако данных о ее приемлемости и опыте пользователей по-прежнему недостаточно. В ходе этого исследования была проведена оценка приемлемости и опыта использования ОВЗ среди взрослых пациентов с МИ, прошедших аллогенный СХТ. Участники использовали прикладную программу VRH и оценили свой опыт с помощью стандартизированных вопросников и полуструктуриров

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… When asked about the choice of landscapes scrolling during hypnosis, participants reported liking landscapes with water, such as the sea and lakes (5/10) or forest landscapes (5/10). Mountains were also suggested (2/10). Complementary Additions. …

The 'Surprise' Question in Haemato-Oncology: The Estimating Physician and Time to Death Reduce the Prognostic Uncertainty-An Observational Study.

Background/objectivesPatients with haematological malignancies less frequently receive specialist palliative care, although they may have unmet needs for symptom control and alleviating psychosocial and existential burdens. The 'Surprise' Question, 'Would you be surprised if this patient died in the next 12 months?', helps physicians to identify patients who may benefit from palliative care. We tested the influencing factors of the feasibility of the 'Surprise' Question in haemato-oncology outpatients.MethodsWe performed a prospective cohort study comparing patients with solid tumours and haem

Внеклеточный маркер изменяется в связи с болезнью при повторном выпуске нескольких склерозов.

Множественный склероз (MS), расстройство спектра нейромиелита (NMOSD) и гликопротеиновое заболевание, связанное с антителами (MOGAD), являются аутоиммунными расстройствами CNS, вызывающими серьезные нейрологическое расстройство. Имеющиеся данные свидетельствуют о том, что внеклеточные везикулы (ЭМ) могут играть конкретную роль в оркестре реакции иммунных клеток MS, NMOSD и MOGAD. Кроме того, ЭМ рассматриваются в качестве потенциального источника биомаркеров, которые могут позволить нам установить молекулярные сигнатуры для этих заболеваний и, возможно, следить за последствиями лечения и прогре

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… Captured EVs were measured with the BD LSRFortessa (BD Biosciences, Franklin Lakes, NJ). Results were analyzed in FlowJo (v. 10.10.0) to obtain median signal intensities for all EV surface markers. …

Health care costs among patients with hematologic malignancies receiving allogeneic transplants: a US payer perspective

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Cardiotoxicity of Non-Anthracycline Cancer Chemotherapy Agents.

Throughout the last decades, newly developed chemotherapeutic agents and immunotherapies that target signaling pathways have provided patients with better prognoses, improved their quality of life and increased survival rates, thus converting cancer to a stable chronic disease. However, non-anthracycline cancer chemotherapy agents and immunotherapies including human epidermal growth factor receptor 2 (HER2) inhibitors, vascular endothelial growth factor (VEGF) inhibitors, Bcr-Abl tyrosine-kinase inhibitors (TKI), proteasome inhibitors, immune checkpoint inhibitors and chimeric antigen receptor

Telehealth exercise to Improve Physical function and frailty in patients with multiple myeloma treated with autologous hematopoietic Stem cell transplantation (TIPS): protocol of a randomized controlled trial.

BACKGROUND: Advances in autologous hematopoietic stem cell transplantation (HSCT) and supportive care have led to marked improvements in survival for patients with multiple myeloma. Despite these improvements, patients with multiple myeloma remain at high risk of physical dysfunction and frailty due to HSCT and its associated exposures. Although traditional supervised exercise programs can improve frailty in cancer patients and survivors, rehabilitation facilities are typically far from a patient's residence, are offered on fixed days/hours, contain uniform activities for everyone, and carry a

The impact of a hospital-based exercise oncology program on cancer treatment-related side effects among rural cancer survivors.

PurposeTo assess the impact of the Personal Optimism With Exercise Recovery (POWER) program on cancer treatment-related side effects among rural cancer survivors.MethodsIn this retrospective study of data collected between 2016 and 2019, we assessed change in cardiorespiratory fitness, whole-body muscular endurance, physical function and strength, anthropometrics, fatigue, and quality of life (QoL), after participation in POWER. Descriptive statistics were calculated for demographic and clinical variables. Univariate analysis of variance was carried out with age and BMI at initial assessment a

LncRNA NORAD promotes bone marrow stem cell differentiation and proliferation by targeting miR-26a-5p in steroid-induced osteonecrosis of the femoral head.

BACKGROUND: Steroid-induced osteonecrosis of the femoral head (SONFH) is a devastating orthopedic disease, which seriously affects the quality of life of patients. The study aims to investigate the effects of LncRNA NORAD on SONFH. METHODS: Human bone marrow-derived mesenchymal stem cells (hBMSCs) were isolated from the proximal femur of patients during routine orthopedic surgery and then cultured with dexamethasone (Dex) and transfected with NORAD overexpression vector, siRNA-NORAD and miR-26a-5p mimics. The mRNA expression of NORAD, miR-26a-5p, OPG, RANK, and RANKL was detected by RT-qPCR.

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… Then, the flow cytometry (BD Biosciences, Franklin Lakes, NJ, USA) was used to analyze cell apoptosis. Statistical analysis All experimental data were expressed as mean ± standard deviation (SD), and GraphPad Prism 7.0 software (USA) was used to perform statistical analysis. …

Impact of Galectin-3 Circulating Levels on Frailty in Elderly Patients with Systolic Heart Failure.

Background: Heart Failure (HF), a leading cause of morbidity and mortality, represents a relevant trigger for the development of frailty in the elderly. Inflammation has been reported to play an important role in HF and frailty pathophysiology. Galectin-3 (Gal-3), whose levels increase with aging, exerts a relevant activity in the processes of cardiac inflammation and fibrosis. The aim of the present study was to investigate the potential of Galectin-3 to serve as a biomarker of frailty in HF patients. Methods: 128 consecutive patients aged 65 and older with the diagnosis of systolic HF underw

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… Biomarker Assessments To detect the Gal-3 circulating levels, an additional blood sample was collected after 12 h of fasting using Becton Dickinson Vacutainer Cat Plus REF 367,896 for serum (BD Diagnostics, Franklin Lakes, NJ, USA). …

CD28 Deficiency Ameliorates Blast Exposure-Induced Lung Inflammation, Oxidative Stress, Apoptosis, and T Cell Accumulation in the Lungs via the PI3K/Akt/FoxO1 Signaling Pathway.

Although CD28 is associated with the expression of inflammatory mediators, apoptosis-related protein, immunosuppression, and tumorigenesis, the effects of CD28 deficiency on blast exposure-induced lung injury have not been investigated. In this study, we have explored the effects of CD28 on blast exposure-induced lung injury and studied its potential molecular mechanisms. A mouse model of blast exposure-induced acute lung injury was established. Sixty C57BL/6 wild-type (WT) and CD28 knockout (CD28-/-) mice were randomly divided into control or model groups. Lung tissue samples were collected 2

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… Single cell suspensions were preincubated with anti-mouse CD3 or CD28 antibodies (BD Biosciences, Franklin Lakes, NJ, USA) to prevent nonspecific binding of antibodies to FcR γ , followed by multistaining with fluorescence conjugated primary antibodies. …

Targeting Notch-1 positive acute leukemia cells by novel fucose-bound liposomes carrying daunorubicin.

Complete remission by induction therapy in acute myelogenous leukemia (AML) can be achieved due to improvements in supportive and optimized therapy. However, more than 20% of patients will still need to undergo salvage therapy, and most will have a poor prognosis. Determining the specificity of drugs to leukemia cells is important since this will maximize the dose of chemotherapeutic agents that can be administered to AML patients. In turn, this would be expected to lead to reduced drug toxicity and its increased efficacy. We targeted Notch-1 positive AML cells utilizing fucose-bound liposomes

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… The mean fluorescence intensity (MFI) was assessed on a FACScalibur with CellQuest software (Becton Dickinson, Franklin Lakes, NJ, USA). …

Levels of active tyrosine kinase receptor determine the tumor response to Zalypsis.

BackgroundZalypsis(®) is a marine compound in phase II clinical trials for multiple myeloma, cervical and endometrial cancer, and Ewing's sarcoma. However, the determinants of the response to Zalypsis are not well known. The identification of biomarkers for Zalypsis activity would also contribute to broaden the spectrum of tumors by selecting those patients more likely to respond to this therapy.MethodsUsing in vitro drug sensitivity data coupled with a set of molecular data from a panel of sarcoma cell lines, we developed molecular signatures that predict sensitivity to Zalypsis. We verified

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… Four to six week-old athymic nu/nu mice (Harlan Sprague Dawley) were s.c. xenografted into their right flank with approx. 0.5-1 × 10 7 cells in 0.2 ml of a mixture (50:50; v:v) of Matrigel basement membrane matrix (Beckton Dickinson, Franklin Lakes, NJ, USA) and serum-free medium. …

Classification of Dystonia.

Dystonia is a hyperkinetic movement disorder characterized by abnormal movement or posture caused by excessive muscle contraction. Because of its wide clinical spectrum, dystonia is often underdiagnosed or misdiagnosed. In clinical practice, dystonia could often present in association with other movement disorders. An accurate physical examination is essential to describe the correct phenomenology. To help clinicians reaching the proper diagnosis, several classifications of dystonia have been proposed. The current classification consists of axis I, clinical characteristics, and axis II, etiolo

ICIMH 2022 Abstracts

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Сосуществование Лактиплантацилла и Виккерхамомиц в верхнем слое сахарного тростника стимулирует потенциальное подавление патогенов и аэробную стабильность за счет увеличения производства лактической кислоты и уксусной кислоты.

Чтобы определить сосуществующие бактерии и дрожжи в верхнем слое сахарного тростника, которые определяют качество ферментации и повышают безопасность корма, мы манипулировали эпифитической микробиотой сверху сахарного тростника путем селективного подавления дрожжей (АФ) или бактерий с лактической кислотой (АБ) перед повторной цензурой, используя в качестве контроля неочищенные (КК) и стерилизованные стерилизованные силизы (НА). Результаты показывают, что высокоурожайное производство лактической кислоты и уксусной кислоты (p) < 0.05), лучшая аэробная стабильность и высокая плотность Лактипланта