Результаты поиска

Mycoplasma infection can sensitize host cells to apoptosis through contribution of apoptotic-like endonuclease(s).

Mycoplasma infection may lead to various pathologies in a broad range of hosts. It has been shown that Mycoplasma may trigger cell death in cell cultures; however, the mechanism remains unknown. In the present paper we show that Mycoplasma infection of different lymphocyte and epithelial tumour cell lines leads to the inhibition of proliferation, and increased cell death, accompanied by DNA fragmentation and the morphological features of apoptosis. We also showed that this infection leads to an increased sensitivity of cells to various inducers of apoptosis targeting different signalling pathw…

Antibodies to HLA class I alpha1 domain trigger apoptosis of CD40-activated human B lymphocytes.

We analyzed herein whether antibodies to HLA class I alpha1 domain, which trigger apoptosis of activated T cells, may also control the growth/survival of human B lymphocytes. Addition of monoclonal antibody (MoAb) 90 (mouse IgG1) or YTH862 (rat IgG2b) was found to strongly inhibit the proliferation of CD40-activated total tonsil B cells as well as that of purified naive, germinal center, and memory B-cell subsets. This inhibitory effect was not prevented by addition of B-cell tropic factors, such as interleukin-2 (IL-2), IL-4, and IL-10, and was a result of induced B-cell apoptosis as shown by…

PUVA treatment selectively induces a cell cycle block and subsequent apoptosis in human T-lymphocytes.

Psoralen plus UVA (320-400 nm radiation; PUVA) is a highly effective therapy for cutaneous diseases caused by skin infiltration with normal … or nuclear fragments, and the appearance of a sub-G1 DNA peak indicated that cell death occurred by apoptosis, beginning about 1 day after PUVA treatment and continuing for several days thereafter. From assessment of cell cycle progression in mimosine-synchronized cells, PUVA treatment markedly slowed cell cycle progression, eventually producing cell cycle …

Bcl-2 protects from lethal hepatic apoptosis induced by an anti-Fas antibody in mice.

Fas is an apoptosis-signalling cell surface antigen that has been shown to trigger cell death upon specific ligand or antibody binding. Treatment of mice with an anti-Fas antibody causes fulminant hepatic failure due to massive apoptosis. To test a putative protective effect of the anti-apoptotic Bcl-2 protein, transgenic mice were generated to express the human bcl-2 gene product in hepatocytes. Early onset of massive hepatic apoptosis leading to death was observed in all nontransgenic mice treated with an anti-Fas antibody. By contrast, hepatic apoptosis was delayed and dramatically reduced …

Interleukin-4 induces programmed cell death (apoptosis) in cases of high-risk acute lymphoblastic leukemia.

We investigated the effects of interleukin-4 (IL-4) on the survival of leukemic and normal B-cell progenitors cultured on bone marrow stroma … e susceptible to IL-4 toxicity. IL-4 cytotoxicity resulted from induction of programmed cell death (apoptosis); there was no evidence of cell killing mediated by T, natural killer, or stromal cells. IL-4 cytotoxicity extended to a proportion of normal B-cell progenitors. After 7 days of culture with IL-4 at 100 U/mL, fewer CD19+, CD34+ normal lymphoblasts ( …

Wild-type p53 induces apoptosis in a Burkitt lymphoma (BL) line that carries mutant p53.

All Burkitt lymphoma (BL) biopsies and cell lines carry a c-myc/Ig translocation. The resulting constitutive activation of c-myc is regarded … of viable cells while the parental and neo control cells remained unaffected. Cell death was due to apoptosis as shown by chromatin and nuclear condensation and specific DNA fragmentation. The first signs of apoptosis were evident after 10 h at 32 degrees C and after 3 days 90-100% of the cells had undergone apoptosis. These findings indicate an incompatibi …

Irradiated fibroblasts protect Burkitt lymphoma cells from apoptosis by a mechanism independent of bcl-2.

When cells of fresh Burkitt lymphoma (BL) biopsies are explanted into tissue culture, their survival and growth are greatly dependent on the … t greatly reduced FCS concentration. A decrease in either cell density or FCS concentration induced apoptosis. Supernatants from feeder cells and from BL70 cells growing autonomously at high cell density were unable to substitute for the survival and growth-promoting effect of the feeder cells. Protection of Burkitt lymphoma cells from apoptosis by co-culti …

Клетки B вызывают апоптоз с помощью нового механизма в фибробластах, инфицированных вирусом гепатита мыши.

В клетки неиммунных мышей служат посредником в цитолизе фибробатластов, инфицированных коронавирусом, вирусом гепатита мыши (МГВ), штаммом А59. В этом исследовании мы сообщаем, что селенические клетки B и гибридома B привели к фрагментации ДНК, зараженной MHV, в нуклеозную лестницу, характерную для апоптоза. Для определения механизма, с помощью которого клетки B содействовали этому убийству, мы использовали критерии, которые ранее были установлены для убийства целевых клеток цитотоксичными лимфоцитами (КТЛ), и сравнили это убийство с убийством с помощью клеток В с помощью лимфоцита (LCMV) с ко

Совпадение в аннотации оригинала
B cells from nonimmune mice mediate the cytolysis of fibroblasts infected with the coronavirus, mouse hepatitis virus (MHV), strain A59. In this investigation, we report that splenic B cells and a B cell hybridoma induced the fragmentation of MHV-infected target cell DNA into a nucleosomal ladder pattern, characteristic of apoptosis. To determine the mechani…

Oral antilymphocyte activity and induction of apoptosis by 2-chloro-2'-arabino-fluoro-2'-deoxyadenosine.

2-Chlorodeoxyadenosine (CdA) is active in chronic lymphocytic leukemia, hairy-cell leukemia, and low-grade lymphomas. In part, this spectrum … he initial DNA damage initiated the pattern of oligonucleosomal DNA fragmentation characteristic of apoptosis. Mutant lymphoblasts, deficient in deoxycytidine kinase, with elevated cytoplasmic 5'-nucleotidase, or with expanded deoxynucleotide pools secondary to increased ribonucleotide reductase activity, were cross-resistant to both CAFdA and CdA toxicity. …

Apoptosis (cell death) induced in mouse bowel by 1,2-dimethylhydrazine, methylazoxymethanol acetate, and gamma-rays.

Apoptosis is a pattern of cell death involving nuclear pyknosis, cytoplasmic condensation, and karyorrhexis. The frequency of apoptosis after treatment with two colon carcinogens and radiation was studied in the crypts of five different portions of mouse bowel. When 1,2-dimethylhydrazine (DMH) was injected s.c., the earliest rise in apoptotic incidence after a high dose (200 mg/kg) was noted at 3 h in small intestine and at 6 h in large bowel. After i.p. administration of methylazoxymethanol (MAM) acetate, apoptotic cells were seen in large bowel after 3 h. When the plateau values attained aft…

Tunlametinib Suppresses Melanogenesis Induces Cell Cycle Arrest and Apoptosis in Giant Congenital Melanocytic Nevus Cells.

Background/Objectives: Giant congenital melanocytic nevus (GCMN) is a rare developmental disorder arising from abnormal proliferation and di … was assessed via Fontana-Masson staining, Western blotting, and immunofluorescence. Cell viability, apoptosis, and cell cycle distribution were evaluated using the CCK-8 assay, flow cytometry, TUNEL staining, and Western blotting for apoptosis- and cell cycle-related proteins. Results: Tunlametinib treatment significantly suppressed melanogenesis in GCMN ce …

SNCA Overexpression Induces Apoptosis in Non-Small Cell Lung Cancer via Caspase-Dependent Signaling Pathways.

The alpha-synuclein (SNCA) gene is a Parkinson's disease (PD)-associated gene that is found to be downregulated in non-small cell lung cance … ptimized CA/plasmid complexes. SNCA overexpression promoted oxidative stress-induced cell death and apoptosis in both lung adenocarcinoma (LUAD) and lung squamous cell carcinoma (LUSC) cells. Additionally, SNCA overexpression activated distinct caspases' activities in LUAD and LUSC. Besides, this study reveals that LUSC was more susceptible to the anticance …

Aleutian mink disease virus (AMDV) enhances viral replication by downregulating JNK protein to promote apoptosis.

Aleutian mink disease virus (AMDV) is a pathogen that causes severe illness in minks, leading to substantial economic losses in the mink far … nt. Phosphorylation of JNK and c-Jun also decreased during infection. Overexpressing JNK attenuated apoptosis and G1 arrest, reversed p53 and p21 induction, restored CDK4, and suppressed viral titers and genome copynumbers; JNK silencing had the opposite apoptotic effect. Together, these results show that JNK restricts AMDV in F81 cells and that the virus i …

Mitochondrial transplantation attenuates apoptosis and improves spermatogenic function following testicular torsion-detorsion injury in rats.

BACKGROUND: Testicular torsion is a urological emergency that may result in irreversible ischemia-reperfusion injury and longterm impairment … usion injury in experimental testicular torsion. By preserving spermatogenic architecture, reducing apoptosis, and restoring progressive sperm motility, mitochondrial therapy represents a promising experimental strategy for fertility preservation in ischemic testicular disorders.

A novel dibenzoylmethane derivative (IDPIP) impairs melanoma cell migration and selectively induces apoptosis in vitro.

Melanoma remains one of the most aggressive forms of skin cancer, with limited therapeutic options and significant treatment-related toxicit … avorable therapeutic window. Morphological and flow cytometric analyses revealed that IDPIP induces apoptosis in a dose-dependent manner (up to 90% mortality), involving activation of both intrinsic and extrinsic pathways, as evidenced by caspase-9 and caspase-8 activation assay, respectively. In addition to its cytotoxic effects, IDPIP impaired melanoma ce …

Oregano extract induces apoptosis and inhibits autophagy in HepG2 cells via the PI3K/AKT pathway.

Hepatocellular carcinoma (HCC) remains one of the most common and highly lethal malignancies globally. Conventional therapeutic approaches a … ur findings demonstrate that EAO markedly suppressed HepG2 and Huh7 cell proliferation and promoted apoptosis, accompanied by increased reactive oxygen species (ROS) generation, loss of mitochondrial membrane potential, and ATP depletion. Additionally, EAO inhibited autophagy, as indicated by decreased LC3-II expression and increased p62 accumulation. Mecha …

MAP30, a type Ⅰ ribosome-inactivating protein, inhibits hepatocellular carcinoma by inducing apoptosis through mitochondria.

BACKGROUND: We have purified Momordica anti-HIV protein of 30 kDa (MAP30), a type I ribosome-inactivating protein from Momordica charantia L … METHODS: The cell counting kit-8 assay was used to detect the proliferation of SK-Hep-1 cells. The apoptosis, cell cycle, reactive oxygen species level and mitochondrial membrane potential of SK-Hep-1 cells were detected by flow cytometry. Protein levels were determined by Western blot. RESULTS: It was confirmed that MAP30 can significantly inhibit the pr …

Cannabidiol Regulates CD47 Expression and Apoptosis in Jurkat Leukemic Cells Dependent upon VDAC-1 Oligomerization.

Background: Cannabidiol (CBD) is a major non-psychoactive phytocannabinoid that exerts multiple biological effects in the body. It has been … e presence or absence of different reagents. Results: CBD downregulates CD47 expression and induces apoptosis in Jurkat cells. Similar biological effects of CBD were also observed in primary human CD4+ T cells, albeit at reduced levels. The CBD's effects on CD47 expression and apoptosis were not rescued by a cannabinoid receptor (CBR)-2 agonist, a CBR-2 ant …

Endothelial-derived TWEAK drives granulosa cell apoptosis in PCOS via the Fn14-oxidative stress axis.

BACKGROUND: Polycystic ovary syndrome (PCOS) is the most common endocrine disorder in reproductive-aged women, characterized by aberrant follicular development and a dysregulated ovarian microenvironment. The roles of intercellular communication, particularly between ovarian endothelial cells and granulosa cells, remain poorly understood. The TWEAK (TNF-like weak inducer of apoptosis)-Fn14 (fibroblast growth factor-inducible 14) axis, implicated in apoptosis and cellular stress, represents a potential but unexplored pathway in PCOS. METHODS: A mouse single-cell RNA-seq dataset (GSE268919) was…

Natural Products Targeting Key Molecular Hallmarks in Gastric Cancer: Focus on Apoptosis, Inflammation, and Chemoresistance.

Natural products have emerged as promising multi-target agents for addressing the complex biology of gastric cancer, a malignancy characterized by marked molecular heterogeneity, late clinical presentation, and frequent resistance to systemic therapies. This narrative synthesis integrates primarily preclinical evidence, with emerging clinical data, on how naturally derived compounds modulate three central molecular processes that drive gastric tumor progression and therapeutic failure: evasion of programmed cell death, persistent tumor-promoting inflammation, and chemoresistance. Compounds suc