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Viability of ovarian tissue after cryopreservation by slow freezing or vitrification in sheep.

Ovarian tissue cryopreservation is a valuable technique for preserving fertility, yet the relative efficacy of vitrification compared to slow freezing remains to be fully explored. This study evaluates the post-thawed viability of ovarian tissue cryopreserved by either method using ovarian samples from healthy ewes aged one year or younger. The ovarian tissue was sectioned into 5 mm × 5 mm × 1 mm slices, cryopreserved, and cultured in vitro for 5 days. Follicle morphology was evaluated via histology, while DNA integrity in primordial follicles and stromal cells was evaluated using TUNEL staini…

Optimization of cryopreservation protocols for cold-adapted yeasts relevant to the brewing industry.

Most studies on yeast cryopreservation have focused on Saccharomyces cerevisiae; therefore, long-term viability and stability of wild strains from other yeast species remain uncertain. Standard protocols typically use glycerol as a cryoprotectant and a CoolCell® container to maintain slow cooling rates. However, limited evidence exists regarding the efficacy of glycerol and the advantages of using controlled-rate containers such as Mr. Frosty™ or CoolCell® compared to direct freezing from ambient temperature to -80 °C or liquid nitrogen (-196 °C) for other yeast species, and particularly for e…

Preliminary studies for elaborating a semen cryopreservation protocol of the Hungarian Giant rabbit.

It is well established that semen freezability varies considerably among rabbit breeds and lines, therefore, breed-specific cryopreservation protocols are required. In the study, three experiments were conducted. Semen quality was assessed based on sperm motility, the proportion of live/dead cells and morphological abnormalities. In Experiment 1, modified Lake's, BotuCrio and INRAFreeze extenders were compared using a simple static cryopreservation protocol. Post-thaw sperm motility was significantly higher with modified Lake's and BotuCrio than with INRAFreeze. However, no significant differe…

Acrosome-Disrupted Dead Sperm Impact the Function of Live Bovine Spermatozoa After Cryopreservation.

The influence of dead sperm on their healthy counterparts in bovine semen is not well established. This is particularly relevant to artificial insemination (AI), since semen handling and biotechnological procedures can increase the percentage of dead sperm. This study aimed to evaluate the impact of acrosome-disrupted (sonicated) spermatozoa on the quality of neighbouring untreated viable cells after cryopreservation. Semen samples from 12 healthy Holstein bulls were diluted (80 × 106 sperm/mL) in pre-warmed OptiXcell extender at 38°C. A 6 mL portion of diluted semen underwent sonication, and …

Sperm cryopreservation of Sebastes schlegelii and application in large scale seed breeding production.

Korean rockfish (Sebastes schlegelii) is a commercially important viviparous teleost widely distributed in China, Japan and Korea, and is characterized by internal fertilization and long-term sperm storage. However, artificial breeding of S. schlegelii has not been well established, and the optimal conditions for sperm preservation remain unclear. In the present study, an effective sperm cryopreservation protocol was successfully developed using 15-20% glycerol as the cryoprotectant combined with a programmable freezer. Post-thaw sperm kinematic parameters were evaluated using a computer-assis…

Ultra-Low Temperature Cryopreservation of Sperm from a New Type of Hybrid Bream.

Sperm cryopreservation is crucial for artificial propagation and the long-term preservation of valuable germplasm resources. This study aimed to establish an effective ultra-low temperature sperm cryopreservation protocol for Hefang bream (HFB), a novel hybrid bream variety developed through distant hybridization between blunt snout bream (Megalobrama amblycephala) and topmouth culter (Culter alburnus) followed by two rounds of backcrossing. Different combinations of extenders and cryoprotectants were evaluated based on post-thaw sperm motility and motion parameters to identify the optimal cry…

Testicular cryopreservation of prepubertal and adult Morada Nova rams: Slow freezing versus vitrification.

Cryopreservation of testis is an alternative for preserving the reproductive potential of prepubertal animals that do not yet produce spermatozoa or endangered adult animals that die unexpectedly. This study aimed to evaluate slow freezing (SFR) and vitrification (VIT) for testicular tissue cryopreservation in prepubertal and adult Morada Nova rams. Small testicular fragments (3 ×3 ×1 mm) were maintained fresh (CTR) or cryopreserved using Mr. Frosty™ for SFR or the ovarian tissue cryosystem for VIT. Fragments were evaluated for histomorphology, ultrastructure, protein (β-catenin, activated cas…

Ovarian cryopreservation with rapamycin improves fertility restoration in a murine orthotopic transplantation model.

Currently, the only fertility preservation option of prepubertal patients is ovarian tissue cryopreservation followed by autotransplantation (OTCTP). Once in remission and patients desire to conceive, autotransplantation of frozen/thawed tissue is performed. A major issue of this technique is follicular loss directly after transplantation, mainly due to follicle activation. Our previous research showed that adding rapamycin to the freezing medium counteracted follicle proliferation and activation induced by OTCTP in heterotopic autotransplantation of ovaries in mice. Our current study aimed to…

Extracellular vesicles as emerging bioactive modulators in sperm cryopreservation: Mechanisms and therapeutic potentials.

Sperm cryopreservation is a cornerstone of assisted reproduction and genetic conservation, yet the freeze-thaw process induces substantial structural and functional damage to spermatozoa. Recent progress in the field has positioned extracellular vesicles (EVs) as potential therapeutic tools, primarily because of their capacity to transport functional biomolecules that help counteract the cellular damage caused by cryopreservation. We then examine the types of cellular and molecular damages encountered during sperm cryopreservation and synthesize current evidence on the protective effects of EV…

Protective effect of exosomes derived from platelet-rich plasma during human sperm cryopreservation.

Fertility preservation is a critical aspect of improving human life quality. Sperm cryopreservation is highly recommended for men at risk of infertility; however, it can have detrimental effects on sperm quality. In this study, we evaluate the effects of platelet-rich plasma (PRP) and PRP-derived exosomes on cryopreservation outcomes in men with asthenoteratozoospermia referred to a public infertility center. The semen samples from 75 men with asthenoteratozoospermia (based on WHO 2020 criteria) were randomly divided into five groups: Control (conventional freezing), Exo/before, Exo/After, PRP…

Prevalence of Fertility Preservation Counseling and Sperm Cryopreservation Amongst Males With Testicular Cancer.

OBJECTIVE: To determine the application of fertility preservation (FP) counseling and sperm cryopreservation (SC) amongst reproductive-aged males diagnosed with testicular cancer using a population-level analysis. METHODS: We retrospectively assessed 16-49-year-olds diagnosed with testicular cancer and treated with orchiectomy between 2015 and 2025 using a large claims database, TriNetX. Primary outcomes were rates of FP counseling and SC within 180 days of testicular cancer diagnosis (90 days before-90 days after). We secondarily assessed rates of utilization within 90 days prior to orchiect…

Oocyte cryopreservation for patients with endometriosis: where are we now? A systematic review.

The ambiguity of current recommendations on the use of oocyte cryopreservation in patients with endometriosis reflects the lack of comprehensive studies evaluating its efficacy. This review seeks to summarize all available data on the outcomes of oocyte cryopreservation and subsequent IVF, while taking patient characteristics into account. The review protocol was registered in PROSPERO (CRD42023486774). The search of electronic databases identified 10 observational studies. Due to their heterogeneity, the extracted data were only analysed descriptively. In terms of oocyte cryopreservation outc…

Antioxidant effect of quercetin and gallic acid in domestic dog epididymal sperm cryopreservation.

Cryopreservation induces negative effects on spermatozoa due to oxidative stress. The aim of this study was to evaluate the effect of the addition of quercetin and gallic acid on sperm characteristics under the cryopreservation process of domestic dog epididymal semen. Samples were collected from sexually mature males by direct epididymal collection technique after orchiectomy and diluted with a commercial extender. Treatments included Control, DMSO (0.005 %), Ethanol (0.05 %), Q25 (quercetin 25 μM), GA45 (gallic acid 45 μM), and a combination of Q25 + GA45. Motility parameters were determined…

Comparative analysis of the histological architecture of ovarian tissue following slow-freezing cryopreservation.

OBJECTIVE: The aim of this study was to validate the methodology of ovarian tissue cryopreservation using the slow freezing technique as a fertility preservation approach and to assess its potential implementation in clinical practice at the Assisted Reproduction Centre of the University Hospital Olomouc. In parallel, the technical procedure of cryopreservation was optimized and standardized. MATERIALS AND METHODS: The study was conducted between April 2022 and December 2024 at the Department of Obstetrics and Gynecology, University Hospital Olomouc, and included six transgender patients aged…

Криопстатизация первичных олигодендроцитов неонатальной крысы и перерасчет характеристик in vitro oligodendrocyte.

ВВЕДЕНИЕ: Внутривитро первичные олигодендроциты крысы широко используются для исследований в области развития ОЛ, физиологии и патофизиологии в таких демиелинизирующих заболеваниях, как множественный склероз. С течением времени были разработаны и усовершенствованы методы первичной культуры ОН у крыс, однако все еще существуют многочисленные аспекты, в которых эффективность может быть повышена. С целью использования излишних клеток потомства олигодендроцитов (ОПК) из первичных культур был установлен процесс криопатирования с использованием доступного на коммерческой основе свободного от сыворот

Совпадение в аннотации оригинала
INTRODUCTION: In vitro, primary rat oligodendrocytes (OLs) are widely used for research on OL development, physiology, and pathophysiology i … d. METHODS: To make use of excess oligodendrocyte progenitor cells (OPCs) from primary cultures, a cryopreservation process utilizing a commercially available serum-free cryopreservation medium was established to passage and freeze OPCs at -80°C for later use. RESULTS: Cryopreserved OPCs stored for up to 6 months were viable, and retained their OL lineage purity …

Cryopreservation of cerebrospinal fluid cells preserves the transcriptional landscape for single-cell analysis.

Cerebrospinal fluid (CSF) matrix biomarkers have become increasingly valuable surrogate markers of neuropsychiatric diseases in research and clinical practice. In contrast, CSF cells have been rarely investigated due to their relative scarcity and fragility, and lack of common collection and cryopreservation protocols, with limited exceptions for neurooncology and primary immune-based diseases like multiple sclerosis. the advent of a microfluidics-based multi-omics approach to studying individual cells has allowed for the study of cellular phenotyping, intracellular dynamics, and intercellular…

Cryopreservation of Human Adult Ventricular Tissue for the Preparation of Viable Myocardial Slices.

Living myocardial slices (LMS) are ultrathin sections of adult myocardium that can be maintained in culture. These slices provide a unique p … een CMs in all LMS after 24 h and up to 5 days of culture in the absence of electrical stimulation. Cryopreservation extends the interval between tissue collection and LMS preparation, facilitating longer-term and more complex experiments. Further research into the impact of cryopreservation on various cardiac cell types will promote better donor organ management …

Chemotherapy and cryopreservation affects DNA repair foci in lymphocytes of breast cancer patients.

PURPOSE: Although breast cancer (BC) patients benefit from radiotherapy (RT), some radiosensitive (RS) patients suffer from side effects cau … t is not always possible to analyze blood samples immediately after collection, there is a need for cryopreservation of PBL in liquid nitrogen. However, cryopreservation may potentially affect the number of DNA repair foci. In this work, we studied the effect of cryopreservation and CHT on the amount of DNA repair foci in PBL of BC patients undergoing radiotherapy …

Rat thyroid graft transplantation after cryopreservation with scintigraphic standardization for an experimental study.

PURPOSE: Budget cuts among other factors undermine the use of state-of-the-art equipment by many research groups. This doesn't mean that their scientific data are not reliable or top-notch. Resort to adaptations is a recurrent need in their reality. The aim of this study was to assess whether scintigraphy with 99mTcO4 is effective in evaluating the functionality of thyroid grafts after cryopreservation in rats. MATERIAL AND METHODS: 24 rats were randomly distributed into 3 groups: Control Group (CG), without surgical procedure, Hypothyroidism Group (HTG), submitted to total thyroidectomy, and…

Study on cryopreservation of Guanzhong dairy goat semen with bovine semen seminal plasma.

There are phospholipase and yolk agglutinase in the seminal plasma of dairy goat. The existence of these enzymes will hydrolyze lecithin in … HOST), oxidation index determination and in vivo fertilization verification test to study the semen cryopreservation of Guanzhong dairy goat. The results showed that if we used bovine seminal plasma instead of 100% dairy goat seminal plasma, the sperm motility, plasma membrane functionality and acrosome integrity rate were the highest after thawing, reaching 67.04 …