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Personalized Radionuclide Therapy via Avidin-Biotin Pretargeting: Biodistribution and Dosimetry of 177Lu-DOTA-biotin with AvidinOX™ in Solid Tumors.

PURPOSE: AvidinOX™ forms a long-lasting artificial receptor that supports effective in vivo binding of circulating biotinylated therapeutic 177Lu-DOTA-biotin. We investigated the biodistribution and radiation dosimetry of 177Lu-DOTA-biotin in patients with solid tumors following image-guided intratumoral AvidinOX administration. METHODS: Three patients received intratumoral injections of AvidinOX and two cycles of intravenous administration of 177Lu-DOTA-biotin over a period of 2 weeks. Multi-time-point whole-body planar and SPECT/CT imaging was performed post infusion. Blood and urine sample…

Biotin Ligase-Based Reporter for Detecting Protease Activity.

Protease cleavage is a crucial process, associated with cellular signaling and viral infection. Here, we present a novel sensing motif based on a proximity-dependent biotin ligase, TurboID. The sensor, Biotin Annotating Genetically Encoded Ligase-based Sensor (BAGELS), was created by engineering TurboID such that it is only active following protease activity, linking cleavage events to the biotinylation of proximal proteins. BAGELS produces a signal-to-background ratio of approximately 48 when tested with and without protease. This reporter represents the first protease activity sensor with bi…

A Higher-Biotin Prenatal Multivitamin Formulation Improves Maternal and Cord Blood Biotin Biomarkers: A 24-Week Double-Blind, Randomized, Controlled Trial.

This double-blind, randomized, controlled trial primarily aimed to determine how supplementation of a prenatal multivitamin with biotin levels above the Adequate Intake (AI) affected biotin status in mother-fetus dyads. Additionally, the correlation between biotin status and stress markers was also explored. Eighty healthy pregnant women were recruited between gestational week 12 and 16. They received a prenatal multivitamin containing either 150 μg (intervention) or 30 μg (control) biotin for 24 wk. Sixty-two participants completed the study. Fasting blood samples were collected at week 0, 12…

Потенциальная роль биотина в нейро-абилитации: обзор.

ЦЕЛЬ ОБЗОРА: Биотин - это растворимый в воде витамин, который млекопитающие не могут синтезировать и должны получать из рациона питания. Помимо своей установленной роли кофактора карбоксилазы, зависящих от биотина, биотин привлекает неврологический интерес, поскольку промежуточный метаболизм, обращение с липидами и техническое обслуживание миелина биологически взаимосвязаны. В настоящем описательном обзоре изучается биотин в трех различных контекстах воздействия - достаточность питания, функциональная недостаточность и фармакологические добавки - и оценивается его предполагаемая значимость для

Совпадение в аннотации оригинала
PURPOSE OF REVIEW: Biotin is a water-soluble vitamin that mammals cannot synthesize and must obtain from the diet. Beyond its established role as a cofactor for biotin-dependent carboxylases, biotin has attracted neurological interest because intermediary metabolism, lipid handling, and myelin maintenance are biologically linked. This narrative review examin…

Biotin for Hair Loss: Teasing Out the Evidence.

OBJECTIVE: Biotin has widespread popularity as a hair supplement. We sought to review the literature regarding biotin's efficacy as a hair supplement. METHODS: We conducted a literature search of PubMed for articles specifically studying the use of oral biotin for hair growth or quality. Case reports and case series were excluded. RESULTS: Three studies met our inclusion criteria. The first study was the highest quality, with a double-blind and placebo-controlled study design, but their results found no difference between the biotin and placebo groups for hair growth. The other two studies i…

Update on Biotin Therapy in Dermatology: Time for a Change.

Biotin (vitamin B7 or H) is found in milk, nuts, egg yolks, cereals, supplements, synthesized by intestinal bacteria, and is required for gluconeogenesis, fatty acid synthesis and amino acid catabolism.

Biotin and carnitine profiles in preterm infants in Japan.

BACKGROUND: Biotin plays an important role as a covalently bound coenzyme for carboxylases. Carnitine is essential in β-oxidation to transport long-chain fatty acids across the inner mitochondrial membrane. The present study was conducted to assess the risk of biotin and carnitine deficiencies in preterm infants who received enteral feeding with maternal milk and/or standard infant formula made in Japan. METHODS: Forty-six infants were enrolled in the study. Urine and serum samples and dried blood spots were collected at 1 week and 1 month of age. Additionally, samples were collected at 40 an…

A streptavidin-biotin-based microarray platform for immunoassays.

The authors describe a microarray system for disease diagnosis based on antibody-antigen interactions. Either biotinylated antibodies or antigens are coupled via streptavidin linkers onto a gold surface. This platform has been used to establish recombinant antibody-antigen interactions and to detect specific IgM antibodies in sera of patients suspected of Lyme borreliosis. Therefore, this microarray system can be adapted for further applications.

Structure-function relations of biotin derivatives of apamin.

Apamin was biotinylated at various residues so as to produce an apamin derivative that was suitable for labelling apamin sensitive K+ channels. We labelled the sole histidine residue (His-18) of apamin with diazobenzoyl biocytin (DBB) and Lys-4 with NHS-biotin and NHS-XX-biotin. We found that at least two labelled species were produced by DBB. Proton NMR spectroscopy revealed that in addition to labelling the His-18, DBB labelled the Gln-16 and Gln-17 of apamin. Both NHS-biotin and NHS-XX-biotin appeared to specifically label Lys-4. To test the potency of these apamin derivatives, we developed…

Labeling of platelet surface glycoproteins with biotin derivatives.

We describe a non-radioactive method for the labeling of platelet surface proteins, consisting of platelet protein biotinylation by means of N-hydroxysuccinimido-biotin (NHS-B) and biotin-hydrazide (H-B); NHS-B labels proteins amino residues while H-B binds to periodate-modified sialoglycoproteins. Washed platelets were biotinylated and protein bands were detected after SDS-electrophoresis and western-blot using avidin-peroxidase and luminol as substrate to enhance the signal which was then detected by X-ray film. Biotin-labeled platelet proteins were also immunoprecipitated with monoclonal an…

A Streptomyces megacluster encodes synergistic biotin-targeting antibiotics.

Natural products remain a major source of antibiotics, but discovery efforts have traditionally treated biosynthetic gene clusters as source … idins, acidomycin, dapamycins, and 2-methyl-7-keto-8-aminopelargonic acid (α-Me-KAPA)-alongside the biotin-binding protein streptavidin. These components converge on bacterial biotin metabolism through complementary mechanisms, including enzyme inhibition, prodrug activation, cofactor mimicry and biotin sequestration. The encoded metabolites are co-produ …