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The effect of proflavin, ethidium bromide and an elevated temperature on the appearance of nisin-negative clones in nisin-producing strains of Streptococcus lactis.

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Selective detection of viable Helicobacter pylori using ethidium monoazide or propidium monoazide in combination with real-time polymerase chain reaction.

Because Helicobacter pylori has a role in the pathogenesis of gastric cancer, chronic gastritis and peptic ulcer disease, detection of its viable form is very important. The objective of this study was to optimize a PCR method using ethidium monoazide (EMA) or propidium monoazide (PMA) for selective detection of viable H. pylori cells in mixed samples of viable and dead bacteria. Before conducting the real-time PCR using SodB primers of H. pylori, EMA or PMA was added to suspensions of viable and/or dead H. pylori cells at concentrations between 1 and 100 μM. PMA at a concentration of 50 μM in…

Application of the SwitchSense Technique for the Study of Small Molecules' (Ethidium Bromide and Selected Sulfonamide Derivatives) Affinity to DNA in Real Time.

The discovery and introduction of the switchSense technique in the chemical laboratory have drawn well-deserved interest owing to its wide r … of the switchSense technique for the study of the binding kinetics of small model molecules such as ethidium bromide (EB) and selected sulfonamide derivatives with DNA in the static and dynamic modes at three different temperatures (15, 25, and 37 °C) each. The experimental results remain in very good agreement with the molecular dynamics docking ones. The …

Molecular encapsulation of berberine and ethidium bromide in anthraquinonecarboxamido-β-cyclodextrin conjugate: supramolecular association with DNA duplex and G-quadruplexes.

G-quadruplex DNA in recognized as a potential target for anti-cancer drugs. In this work, an anthraquinonecarboxamido derivative of β-cyclodextrin (AQCC) is synthesized as a novel DNA binder that further can deliver an additional molecule at the target, carrying it in the cavity of modified cyclodextrin. The binding of AQCC with ethidium bromide (EtBr), berberine (Ber), duplex calf-thymus DNA (CT-DNA), quadruplexes (G4) viz., kit22, myc22, and telo24 are studied. The compound acts as a host molecule for the encapsulation of DNA binders viz., EtBr, Ber and enhances their fluorescence due to the…

Comparison of heteroduplex and single-strand conformation analyses, followed by ethidium fluorescence visualization, for the detection of mutations in four human genes.

Non-isotopic DNA single-strand conformation analysis and heteroduplex analysis by ethidium bromide fluorescence visualization (SSCAE and HAE, respectively) were compared for the detection of 15 different naturally occurring mutations in 15 different DNA samples. The mutations included single nucleotide transitions, transversions and deletions, in CFTR (cystic fibrosis transmembrane conductance regulator), COL4A5 (collagen type IV alpha 5 chain), HEXB (hexosaminidase B), and COL1A2 (collagen type 1 alpha 2 chain) genes, responsible for diseases of medical interest. Genomic DNA from peripheral b…

Modulation of phencyclidine-sensitive ethidium binding to the Torpedo acetylcholine receptor: interaction of noncompetitive inhibitors with carbamylcholine and cobra alpha-toxin.

Ethidium is one of two fluorescent ligands known to bind to the noncompetitive inhibitor (NCI) site in the central ion channel of the Torpedo acetylcholine receptor with a micromolar dissociation constant. To further characterize heterotropic allosteric regulation of ligand binding in general, and of ethidium binding in particular, to the Torpedo receptor, we measured the effects of three liquid anesthetics (diethyl ether, halothane, and butanol), two barbiturates (secobarbital and thiamylal), and urethane. The phencyclidine-sensitive chromatic shift and the quantum yield increase associated w…

Forskolin, an Adenylcyclase/cAMP/CREB Signaling Activator Restoring Myelin-Associated Oligodendrocyte Destruction in Experimental Ethidium Bromide Model of Multiple Sclerosis.

Multiple sclerosis (MS) is a chronic neurodegenerative disease marked by oligodendrocyte loss, which results in central neuronal demyelination. AC/cAMP/CREB signaling dysregulation is involved in the progression of MS, including mitochondrial dysfunctions, reduction in nerve growth factors, neuronal inflammation, apoptosis, and white matter degeneration. Our previous research has shown that Forskolin (FSK), a naturally occurring direct adenylyl cyclase (AC)/cAMP/CREB activator, has neuroprotective potential to alleviate pathogenic factors linked with numerous neurological abnormalities. The cu

Preparation of Phi29 DNA polymerase free of amplifiable DNA using ethidium monoazide, an ultraviolet-free light-emitting diode lamp and trehalose.

We previously reported that multiply-primed rolling circle amplification (MRPCA) using modified random RNA primers can amplify tiny amounts … on of host DNA removal using appropriate salt concentrations, inactivation of amplifiable DNA using ethidium monoazide, and irradiation with visible light from a light-emitting diode lamp. Any remaining DNA, which likely exists as oligonucleotides captured by the Phi29 DNA polymerase, is degraded by the 3'-5' exonuclease activity of the polymerase itself i …

DFT and Z-scan Studies for the Determination of Nonlinear Optical Susceptibility and Second Order Hyperpolarizability of Ethidium Bromide Dye in Different Solvents.

Nonlinear optical (NLO) susceptibility of Ethidium Bromide (EB) dye, an excellent NLO material, dissolved in different solvents, including ethanol, methanol, acetone, 1-propanol, DMF, and DMSO is reported. Z-scan tool is used to measure the NLO features of EB dye using open and closed aperture methods. The peak-valley transmittance graph indicating self-defocusing nonlinearity in the nonlinear refractive index of EB dye in different solvents. The nonlinear absorption coefficient showed both positive and negative nonlinearity is the result of saturable and reverse saturable absorption behavior …

Study of interactions between DNA-ethidium bromide (EB) and DNA-acridine orange (AO), in solution, using hanging mercury drop electrode (HMDE).

The interaction of ethidium bromide (EB) and acridine orange (AO) with double stranded (ds), thermally denatured (ss) and supercoiled (sc) DNA, in solution, was studied by alternating current voltammetry (AC voltammetry) at the hanging mercury drop electrode (HMDE) in 0.3 M NaCl+50 mM sodium phosphate buffer (pH 8.5). Their interaction with DNA is shown to be time dependent and completely different. The changes at peak 2 (peak at -1.20 V) of dsDNA form and the appearance of peak 3 (peak at -1.42 V) in scDNA form are presented as criteria declaring the different mechanism of interaction of EB a…

Superoxide reacts with hydroethidine but forms a fluorescent product that is distinctly different from ethidium: potential implications in intracellular fluorescence detection of superoxide.

Hydroethidine (HE) or dihydroethidium (DHE), a redox-sensitive probe, has been widely used to detect intracellular superoxide anion. It is a common assumption that the reaction between superoxide and HE results in the formation of a two-electron oxidized product, ethidium (E+), which binds to DNA and leads to the enhancement of fluorescence (excitation, 500-530 nm; emission, 590-620 nm). However, the mechanism of oxidation of HE by the superoxide anion still remains unclear. In the present study, we show that superoxide generated in several enzymatic or chemical systems (e.g., xanthine/xanthin…

ADMET study, spectroscopic characterization and effect of synthetic nitro chalcone in combination with norfloxacin, ciprofloxacin, and ethidium bromide against Staphylococcus aureus efflux pumps.

Chalcones are present in a wide variety of plants, having in their structure two aromatic rings that are linked together by a chain composed of three carbon atoms with α, β-unsaturated to carbonyl system. Bacteria have several drug resistance mechanisms, among them the efflux pump; this mechanism, when active, is able to expel different compounds from inside bacterial cells. Several efflux pumps have already been identified for Staphylococcus aureus bacteria, including MepA and NorA. Many chalcones have been isolated and identified with various activities, such as antimicrobial. In view of thi

Significance of Fluorescent Spectroscopy in Screening Oral Potentially Malignant Disorders and Oral Cancer by Characterization of Salivary DNA Using Ethidium Bromide-A Comparative Study.

Sangeetha RamamoorthyBackground  Optical diagnosis is one of the upcoming methods in screening and diagnosing oral cancer at the earlier stage. Currently, DNA-based diagnosis is used along with light-based imaging methods to diagnose oral cancer rapidly. Aim  The aim of this study was to discriminate oral cancer and oral potentially malignant disorders from normal patient with fluorescence spectroscopic characterization of salivary DNA using ethidium bromide dye. Materials and Methods  A total of 40 patients with average age of 20 to 60 years in both the genders were selected and divided into …

Nrf2/HO-1 Signaling Stimulation through Acetyl-11-Keto-Beta-Boswellic Acid (AKBA) Provides Neuroprotection in Ethidium Bromide-Induced Experimental Model of Multiple Sclerosis.

Multiple sclerosis (MS) is a severe immune-mediated neurological disease characterized by neuroinflammation, demyelination, and axonal degeneration in the central nervous system (CNS). This is frequently linked to motor abnormalities and cognitive impairments. The pathophysiological hallmarks of MS include inflammatory demyelination, axonal injury, white matter degeneration, and the development of CNS lesions that result in severe neuronal degeneration. Several studies suggested downregulation of nuclear factor erythroid-2-related factor-2 (Nrf2)/Heme oxygenase-1 (HO-1) signaling is a causativ

Изучение роли нейропной защиты дериватива Sulfonamide KM11 в демиелинизации в модели Zebrafish с помощью модуляции антиоксидантов и воспаляющих цитокиновых путей.

Множественный склероз (MS) — гиперчувствительная реакция с повышенным воспалением, что приводит к обезвоживанию и нейронному дегенерации. В настоящее время для повышения способности к воспоминанию имеются ограниченные лекарственные средства. Цель этого исследования заключалась в оценке нейрозащитного и ремиелионирующего потенциала новой производной сульфонамида, KM11 [N-(7-гидрокси-1,3-бензотиазол-2-ил)-4-метилбензол-1-сульфонамид], в модели демиелинации у зебры. KM11 был синтезирован в лаборатории и опробован с использованием комплексного подхода. Клетки SH-SY5Y, обработанные KM11 (10-200 мкм

Совпадение в аннотации оригинала
Multiple sclerosis (MS) is a hypersensitivity reaction with increased inflammation, which leads to demyelination and neuronal degeneration. … ulfonamide derivative, KM11 [N-(7-hydroxy-1,3-benzothiazol-2-yl)-4-methylbenzene-1-sulfonamide], in ethidium bromide (EtBr)-induced demyelination models in zebrafish. KM11 was synthesised in the laboratory and tested using an integrated approach. SH-SY5Y cells treated with KM11 (10-200 µM) were assessed for morphology, viability (MTT), and apoptosis (AO/PI …

Spectroscopic and molecular modeling methods to investigate the interaction between psycho-stimulant modafinil and calf thymus DNA using ethidium bromide as a fluorescence probe.

Interaction type of modafinil with calf thymus DNA (ct-DNA) was examined systematically using ethidium bromide (EB) as a fluorescence probe by fluorescence spectroscopy, UV-Vis spectroscopy, viscosity and molecular docking method. The fluorescence quenching mechanism of ct-DNA-EB by modafinil can be combination of static and dynamic quenching. Results of UV-Vis absorption, competitive binding with Hoechst 33258, ionic strength effect studies, viscosity measurements were confirmed that the interaction type of modafinil with ct-DNA was intercalation. According to docking studies R-modafinil show…

Single UV excitation of Hoechst 33342 and ethidium bromide for simultaneous cell cycle analysis and viability determinations on in vitro cultures of murine B lymphocytes.

Assessment of DNA content by flow cytometry has largely depended on staining techniques which do not permit exclusion of dead cells from the data set. During studies of B cell activation in vitro, the large number of nonviable cells greatly affects the cell cycle distribution and thus the accurate evaluation of proliferation flow cytometry. This report describes the development of two dual staining techniques which use Hoechst 33342 and ethidium bromide excited by a single UV source to eliminate dead cells from the DNA histogram of the viable cells in murine B cell cultures. Hoechst 33342 and …

Multitarget therapeutic potential of sulforaphane in ethidium bromide-induced neurotoxicity in multiple sclerosis-like pathology: comparison with omaveloxolone and dimethyl fumarate on neuroprotection and systemic recovery.

Multiple sclerosis (MS) is a chronic autoimmune disorder characterized by demyelination, neuroinflammation, and neurodegeneration. This study investigates the neuroprotective potential of Sulforaphane (SFN) in ameliorating ethidium bromide (EBRM)-induced MS-like pathology in Wistar rats. The efficacy of SFN at two doses (SFN1.5 and SFN3) was compared to FDA-approved Nrf2 activator drugs, omaveloxolone (OMV15) and dimethyl fumarate (DIMF50). EBRM administration caused neurobehavioral deficits, demyelination, oxidative stress, axonal degeneration, and inflammation. It disrupted key cellular path…

Fast, facile and ethidium bromide-free assay based on the use of adsorption indicator for the estimation of polyethylenimine to nucleic acid ratio of complete polyplex assembly for gene delivery.

A new method was developed for the estimation of polyethylenimine (PEI) to nucleic acid ratio at which the polyplex was completely formed. T … osed method required no sophisticated instruments, time-consuming gel electrophoresis, carcinogenic ethidium bromide as well as costly dyes and the analysis could be accomplished within less than 10 min. Hence, it was a fast, facile, cost-effective and safe-for-operator alternative method, suited for the investigation of the optimal PEI to nucleic acid rat …

Removal of Pb(II) ions and ethidium bromide from aqueous solution using novel magnetic chitosan-based Schiff base composite derived from 2-imino-3-hydroxypiridine methyl benzaldehyde.

Schiff bases compounds can be used for the removal of heavy metal ions from water due to their chelating properties. In this work, magnetic chitosan-(2-imino-3-hydroxypiridine methyl benzaldehyde (MCS-IHPMB) was synthesized and applied in an aqueous solution to remove Pb(II) ions and ethidium bromide (EBr). MCS-IHPMB structural characteristics were studied by spectroscopic analysis including FT-IR, VSM, XRD, SEM, EDX, DLS, and TGA. Batch adsorption experiments were done under various conditions including initial concentrations of dye and metal ion, contact time, pH, and adsorbent dose. Equilib…