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Linear mapping of tryptophan residues in Vesiculovirus M and N proteins by partial chemical cleavage.

Nonlimit chemical cleavage at tryptophan residues of protein labeled at the amino terminus afforded a simple procedure for generating specific fragments and for mapping tryptophan positions. A comparison of the matrix (M) and nucleocapsid (N) proteins of four members of the Vesiculovirus group by this procedure suggests considerable conservation of tryptophan number and location in the four serotypes examined.

[Serological evidence of Lassa virus circulation in the Republic of Guinea].

Seroepidemiological investigations in Guinea were carried out to estimate the areas of Lassa virus circulation. The recombinant protein of Lassa virus nucleocapsid was used as the antigen to analyse blood sera by ELISA. In some regions, from 30 to 54.9% of the population had antibodies to Lassa virus, but in others only 6-7%.

Ex vivo detection of SARS CoV 2 spike protein in human hair follicles and potential link to telogen effluvium.

Telogen effluvium (TE), a form of hair loss, has emerged in a subset of COVID-19 patients, typically occurring several weeks after infection … e's layer of the inner root sheath (IRS). Ex vivo infection of plucked HFs with SARS-CoV-2 revealed nucleocapsid (NC) protein immunoreactivity in the ORS in two of three donors, corresponding to the K15⁺ epithelial stem/progenitor cell population. These NC⁺ cells co-expressed cleaved caspase3 (CAS3) and TUNEL, indicating apoptosis-related changes. Our findings …

Структура и динамика Несочлененного нуклеопротеина Раби-Вируса в комплексе с его модулем фосфопротеина Чаперона.

Что касается всех несегментированных негативных вирусов РНК, то вирус бешенства упакован в линейный блок нуклеопротеина (N), названный нуклеокапсидом. Формирование новых нуклеокапсидов во время репродукции вируса в клетках требует производства растворимого N-белка в комплексе с фосфопротеиновым (P) сопровождающим. В этом исследовании мы восстановили растворимый гетеродимерный комплекс между безручным N-белком бешеного вируса (RABV), не имеющим поддома N-терминалов (NNT-ARM), и пептидом, охватывающим модуль N0-капиталов P-белка. Мы показали, что модуль сопровождающего проходит беспорядочный пер

Совпадение в аннотации оригинала
As for all non-segmented negative RNA viruses, rabies virus has its genome packaged in a linear assembly of nucleoprotein (N), named nucleocapsid. The formation of new nucleocapsids during virus replication in cells requires the production of soluble N protein in complex with its phosphoprotein (P) chaperone. In this study, we reconstituted a soluble heterod…

[First detection of human metapneumovirus in a child with respiratory syndrome in Hungary].

INTRODUCTION: Human metapneumovirus was identified in 2001 as a respiratory-tract pathogen that has been classified as a new genera in family Paramyxoviridae. AIMS: Molecular detection of human metapneumovirus in Hungary. MATERIALS AND METHODS: Human metapneumovirus was identified in nasopharyngeal aspirate amplification of the viral fusion and nucleocapsid genes by reverse transcription-polymerase chain reaction followed by sequencing and phylogenetic analysis. RESULTS: A 4 years-old girl with chronic respiratory syndrome chronically treated with anti-asthma drugs was admitted to hospital …

Long-Term SARS-CoV-2 Antibody Seroprevalence in Blood Donors, Italy.

We evaluated SARS-CoV-2 antibody response in voluntary blood donors in Italy at different timepoints. Immediately after lockdown easing, 908/25,657 donors (3.5%) had low IgG titers against nucleocapsid. In the next 2 years, titers increased despite few COVID-19 symptoms. On multivariate analysis, allergic rhinitis was associated with reduced risk for symptomatic COVID-19.

Serological responses to SARS-CoV-2 in urban and rural Ghana: Antibody waning and implications for long-term population immunity.

BACKGROUND: Ghana exhibits geographic variation across northern and southern regions alongside differences between urban and rural communiti … ctivated and tested with semi-quantitative Anti-SARS-CoV-2 IgG ELISA assays targeting spike (S) and nucleocapsid (N) proteins. Presence of SARS-CoV-2 neutralizing antibodies in serum was determined by a surrogate virus neutralization assay. RESULTS: Of 987 participants aged 10-88 years (67.3% female), SARS-CoV-2 seroprevalence was comparable between urban (47 …

A Targeted LC-MRM3 Proteomic Approach for the Diagnosis of SARS-CoV-2 Infection in Nasopharyngeal Swabs.

Since its first appearance, severe acute respiratory syndrome coronavirus 2 quickly spread around the world and the lack of adequate PCR testing capacities, especially during the early pandemic, led the scientific community to explore new approaches such as mass spectrometry (MS). We developed a proteomics workflow to target several tryptic peptides of the nucleocapsid protein. A highly selective multiple reaction monitoring-cubed (MRM3) strategy provided a sensitivity increase in comparison to conventional MRM acquisition. Our MRM3 approach was first tested on an Amsterdam public health cohor…

Curcumin-derived carbon-dots as a potential COVID-19 antiviral drug.

Even entering the third year of the COVID-19 pandemic, only a small number of COVID-19 antiviral drugs are approved. Curcumin has previously shown antiviral activity against SARS-CoV-2 nucleocapsid, but its poor bioavailability limits its clinical uses. Utilizing nanotechnology structures, curcumin-derived carbon-dots (cur-CDs) were synthesized to increase low bioavailability of curcumin. In-silico analyses were performed using molecular docking, inhibition of SARS-CoV-2 nucleocapsid C-terminal domain (N-CTD) and antiviral activity were assessed in dimer-based screening system (DBSS) and in vi…

Isolation and epitope characterization of human monoclonal antibodies to hepatitis C virus core antigen.

In this study we describe the establishment of two hybridoma cell lines secreting human monoclonal antibodies to the 22-kD nucleocapsid protein (core, p22) of the hepatitis C virus (HCV). For this purpose we isolated B lymphocytes from an anti-HCV positive blood donor and infected them with Epstein-Barr (EBV). We obtained several lymphoblastoid cell clones secreting antibodies to the recombinant HCV core protein. The B-cell cultures were oligoclonally expanded and two of them were fused with the (mouse:human) heteromyeloma cell line K6H6/B5. The resulting stable hybridomas produce antibodies o…

Construction of yellow fever-influenza A chimeric virus particles.

In order to obtain a better understanding of the functional mechanisms involved in the fusogenesis of enveloped viruses, the influenza A (X31) and the yellow fever (17DD) virus particles were used to construct a chimeric structure based on their distinct pH requirements for fusion, and the distinct malleability of their nucleocapsids. The malleable nucleocapsid of the influenza A virus particle is characterized by a pleomorphic configuration when observed by electron microscopy. A heat inactivated preparation of X31 virus was used as a lectin to interact with the sialic acid domains present in…

Natural history of restricted synthesis and expression of measles virus genes in subacute sclerosing panencephalitis.

Subacute sclerosing panencephalitis (SSPE) is a slow infection caused by measles virus in which several years separate recovery from typical … and temporal distribution of virus genes in vivo, using region- and strand-specific probes for the nucleocapsid and matrix genes. We show that early in the course of SSPE there is a global repression in the synthesis and expression of the genome. In the final stage of SSPE most infected cells still have depressed levels of plus- and minus-strand viral RNA and …

[Characteristics of incorporation of carbohydrate radioactivity into structural proteins of the tick-borne encephalitis virus].

The propagation of the tick-borne encephalitis virus in the culture of the porcine embryo kidney cells containing the radioactive mannose and glucosamine results in incorporation of radioactive label into hemagglutinin V3(E) as well as into other structural proteins, the nucleocapsid protein V2(C) and membrane protein V1(M). The possible reasons for carbohydrates borne radioactivity incorporation into the proteins V2 and V1 are discussed.

Effects of L-glutamine deprivation on growth of HVJ (Sendai virus) in BHK cells.

l-Glutamine requirement for viral maturation was found in BHK-HVJ cells, a cell line of baby hamster kidney cells persistently infected with HVJ (Sendai virus). Synthesis of envelope protein in BHK-HVJ cells was markedly suppressed by deprivation of l-glutamine, whereas development of nucleocapsid (S) antigen was less affected. More detailed examination of this phenomenon was carried out by using a cytolytic system. Growth of HVJ in BHK cells cultured in media deprived of various amino acids was investigated, and omission of l-glutamine from culture medium resulted in a marked inhibitory effec…

Development and validation of a recombinant N protein-based indirect ELISA for serological detection of feline infectious peritonitis virus.

Feline infectious peritonitis (FIP), a fatal disorder driven by the feline infectious peritonitis virus (FIPV), has no clinically approved vaccine available to date-rendering prevention and early-stage diagnosis critical to its control. In this study, a feline infection model was developed via intraperitoneal inoculation of virus-laden ascitic fluid, with infection validation conducted using clinical observation, hematological testing, biochemical profiling, imaging examinations, histopathological analysis, and immunofluorescence assay. The viral nucleocapsid N gene underwent cloning and heter…

Facile Synthesis of Lecithin-Sophorolipid Liponiosomes Encapsulating SARS-CoV‑2 Proteins for Therapeutic Applications.

The major structural proteins of SARS-CoV-2 (spike and nucleocapsid) hold higher immunogenicity and have the potential to offer protective immunity. However, development of a biogenic and biocompatible delivery vehicle for subunit vaccine candidates remains a critical gap. The study was aimed to synthesize and evaluate liponiosomes encapsulating SARS-CoV-2 proteins as a novel delivery system. The SARS-COV-2 nucleocapsid and spike proteins were expressed in the mammalian cell line. The purified proteins were encapsulated into novel liponiosome (based on biogenic lecithin-sophorolipid) nanoparti…

Multifocal meningoencephalitis after vaccination against COVID-19.

We report the case of an 84-year-old male patient who was transferred to our hospital because of impaired consciousness and high fever, and … mical analysis for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antigens (spike and nucleocapsid proteins) was performed to identify the cause of death. Surprisingly, only SARS-CoV-2 spike protein was detected in the thalamus, pons, and pituitary and adrenal glands. The presence of SARS-CoV-2 spike protein might have been due to vaccination rat …

Improvement of immune dysregulation in individuals with long COVID at 24-months following SARS-CoV-2 infection.

This study investigates the humoral and cellular immune responses and health-related quality of life measures in individuals with mild to moderate long COVID (LC) compared to age and gender matched recovered COVID-19 controls (MC) over 24 months. LC participants show elevated nucleocapsid IgG levels at 3 months, and higher neutralizing capacity up to 8 months post-infection. Increased spike-specific and nucleocapsid-specific CD4+ T cells, PD-1, and TIM-3 expression on CD4+ and CD8+ T cells were observed at 3 and 8 months, but these differences do not persist at 24 months. Some LC participants …

The Performances of Three Commercially Available Assays for the Detection of SARS-CoV-2 Antibodies at Different Time Points Following SARS-CoV-2 Infection.

The aim of this study was to evaluate the performances of three commercially available antibody assays for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antibodies at different time points following SARS-CoV-2 infection. Sera from 536 cases, including 207 SARS-CoV-2 PCR positive, were tested for SARS-CoV-2 antibodies with the Wantai receptor binding domain (RBD) total antibody assay, Liaison S1/S2 IgG assay and Alinity i nucleocapsid IgG assay and compared to a two-step reference ELISA (SARS-CoV-2 RBD IgG and SARS-CoV-2 spike IgG). Diagnostic sensitivity, specif…

Isolation of Tioman virus from Pteropus giganteus bat in North-East region of India.

Bat-borne viral diseases are a major public health concern among newly emerging infectious diseases which includes severe acute respiratory … le to Tioman virus. Negative electron microscopy study revealed the "herringbone" morphology of the nucleocapsid filaments and enveloped particles with distinct envelope projections a characteristic of the Paramyxoviridae family. Sequence analysis of Nucleocapsid gene of TioV demonstrated sequence identity of 99.87% and 99.99% nucleotide and amino acid respect …