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Potential Biomarkers of Fatal Hypothermia Revealed by UHPLC-MS Metabolomics in Mice.

BACKGROUND: The postmortem diagnosis of fatal hypothermia presents a considerable challenge in forensic medicine. Metabolomics, a powerful t … ssues (spleen: second, liver: tenth, stomach: eleventh, gastrocnemius muscle: twenty-first), as did tryptophan metabolism (spleen: thirteenth, liver: eighth, stomach: third, gastrocnemius muscle: seventeenth). CONCLUSIONS: Our findings provide insights into the metabolic perturbations associated with fatal hypothermia in different tissues and lay a foundati …

Effect of Aloe vera on brain indoleamine 2, 3 dioxygenase activity and tissue antioxidant status in rats subjected to swim stress.

Aloe vera (AV), is a succulent plant with numerous health benefits, including antioxidant, immune-boosting, and antidepressant properties. I … s, mitigating stress-induced depressive states by decreasing brain IDO activity, thereby increasing tryptophan availability for central serotonin synthesis.

Polysaccharides from Scrophularia ningpoensis Hemsl. improve reserpine-induced depression-like behavior by inhibiting HTR2A/HTR2C mediated AKT/GSK3β/β-catenin/CBP/BDNF signalling.

Scrophularia ningpoensis Hemsl. is a traditional Chinese medicine used to regulate blood sugar levels, immunity, etc. We previously isolated … asis and hippocampal neurogenesis. SNPS reversed the depletion of 5-HT, NE and DA by activating the tryptophan (Trp)/5-HT and tyrosine (Tyr)/DA/NE metabolic pathways. SNPS decreased HTR2A and HTR2C contents, leading to the phosphorylation of AKT and GSK3β, followed by increases in β-catenin, CBP and BDNF levels. Mechanistically, SNPS reduced the levels of HT …

Probiotic Supplementation Enhances Functional Recovery and Modulates the Serum Metabolome in Mice.

Bone fractures represent common traumatic injuries associated with significant morbidity due to acute and, in some cases, persistent chronic … systemic metabolic profiles, notably affecting pathways involved in neurotransmitter biosynthesis, tryptophan metabolism, and inflammatory signaling. Collectively, these findings highlight the therapeutic potential of microbiota-targeted strategies as nonpharmacological interventions for improving functional recovery and reducing pain behavior following tra …

Perfluorooctanoic acid at environmentally relevant concentrations interfere with nitrogen fixation and amino acid synthesis in soil-soybean system.

Perfluorooctanoic acid (PFOA) contamination in soils is of concern, stemming from its detrimental impact on ecosystem functions and human he … m, especially decreasing the contents of l-4-hydroxyproline (Hyp), γ-aminobutyric acid (GABA) and l-tryptophan, thereby lowering the nutritional quality of soybeans. It also changed the relative abundance of soil microorganisms, reduced the production of flavonoids, and negatively impacted the biological nitrogen fixation of soybean. The findings unequivocal …

Amino acid metabolites as potential circulating biomarkers for sarcopenia.

Sarcopenia, characterized by the loss of muscle mass and strength, is a multifactorial disorder, including metabolic disturbance. Plasma ami … overy cohort, isoleucine (Ile), leucine (Leu), valine (Val), methionine (Met), phenylalanine (Phe), tryptophan (Trp), alpha-aminoadipic acid (alpha-AAA), glutamate (Glu), and methionine sulfoxide (MetO) were lower in men with sarcopenia, while Glu was lower in women (p < 0.05). Leu in men and Glu in both sexes were associated with skeletal muscle index. A re …

Integrating Docking, Dynamics, and Assays to Predict Antimicrobial Peptide Interactions with Mycolic Acid Membranes in Mycobacterium tuberculosis.

The global burden of multidrug-resistant tuberculosis (MDR-TB) underscores the urgent need for novel therapeutics with distinct mechanisms of action. Here, we report a comparative evaluation of four antimicrobial peptides (AMPs) derived from the amphibian peptide B1CTcu5, integrating experimental validation with molecular modeling to elucidate structure-activity relationships. Among them, W-B1CTcu5, featuring a single N-terminal tryptophan substitution, exhibited the most potent antimycobacterial activity (MIC = 3.2 μg/mL) against Mycobacterium tuberculosis (MTB) combined with high structural …

Capillary Electrophoresis Electrospray Ionization Mass Spectrometry Reveals Metabolic Perturbations During Nematode Infection in Drosophila melanogaster.

Drosophila melanogaster is broadly used to model host-pathogen interactions. Entomopathogenic nematodes are excellent research tools for dis … that the infection was paralleled with changes in amino acid biosynthesis (arginine, phenylalanine, tryptophan, and tyrosine), metabolism (alanine, arginine, aspartate, glutamate, glycine, proline, serine, and threonine), and classical signalling (aspartate, γ-aminobutyrate, glutamate, and pyridoxine). This study demonstrates the ability of high-sensitivity …

Chronic unpredictable mild Stress- induced neurobehavioral and metabolic alteration: insights into molecular mechanisms and emerging therapeutic Strategies".

The chronic unpredictable mild stress (CUMS) paradigm is a well-known preclinical model used to investigate the pathophysiology of stress-in … microbial dysbiosis and intestinal barrier disruption amplify central inflammation through altered tryptophan metabolism and immune neurotransmitter signalling. Simultaneously, epigenetic modification including DNA methylation, histone remodelling and microRNAs encodes transcriptional changes that results in behavioural and cognitive deficits. While, CUMS m …

Glucosinolate Metabolites and Brain Health: обновленный обзор их потенциальных выгод от нейродегенеративных, нейроразвитых и психических расстройств.

Нейродегенеративные, нейроразвитые и психические расстройства, а также эпилепсия затрагивают миллионы людей. В силу их воздействия на качество жизни пациентов они представляют собой серьезную проблему в области здравоохранения. В качестве новых методов лечения этих заболеваний возникают природные соединения. В частности, глюкозинолаты (GLS) представляют собой вторичные метаболиты, встречающиеся на семейных растениях Крюциферае. Их основная структура состоит из глюкозы, соединенной с группой тиогидроксимата-O-сульфоната, и алифатической, аралкиловой или индолиловой боковой цепи в зависимости от

Совпадение в аннотации оригинала
Neurodegenerative, neurodevelopmental, and psychiatric disorders, as well as epilepsy, affect millions of people. Due to their impact on pat … ally, aliphatic GLS derive from methionine, aromatic ones from phenylalanine, and indolic ones from tryptophan. Myrosinase (thioglucoside glucohydrolase) is the crucial enzyme for GLS degradation, leading to the production of isothiocyanates (ITCs). ITCs attracted considerable scientific interest for their protective effects against various diseases, thanks …

Кинуреньенс и митохондриальные расстройства в многоразовом склерозе.

Множественный склероз (MS) — хроническое аутоиммунное заболевание, характеризующееся воспалением, обезвоживанием и нейродегенерацией в центральной нервной системе. Патогенез MS связан с иммунной атаки на миелин и нейроны, сопровождаемой дисфункцией мозга в крови и хроническим воспалением CNS. Центральное значение для патологии MS имеет дисрегулирование кинуреньинового пути, который метаболизирует триптофан в нейроактивные соединения. Активация Kynurenin Translation (KP) под воздействием подстрекательских цитокинов приводит к образованию как нейрозащитных (например, кинуриновой кислоты, КИНА),

Совпадение в аннотации оригинала
Multiple sclerosis (MS) is a chronic autoimmune disease characterised by inflammation, demyelination, and neurodegeneration within the centr … inflammation. Central to MS pathology is dysregulation of the kynurenine pathway, which metabolises tryptophan into neuroactive compounds. Kynurenine pathway (KP) activation, driven by inflammatory cytokines, leads to the production of both neuroprotective (e.g., kynurenic acid, KYNA) and neurotoxic (e.g., quinolinic acid, QUIN) metabolites. Imbalance betwee …

A mannose-rich exopolysaccharide-1 isolated from Bifidobacterium breve mitigates ovalbumin-induced intestinal damage in mice by modulation CD4 + T cell differentiation and inhibiting NF-κB signaling pathway.

Ovalbumin (OVA)-induced intestinal injury is a recurrent and potentially fatal condition. Previous studies have highlighted the roles of exo … omitus. This microbiota modulation enhanced the levels of metabolites such as tyrosine, methionine, tryptophan, triglycerides, and salidroside. In conclusion, EPS-1 shows promise as a functional polysaccharide for therapeutic use.

Association between large neutral amino acids and white matter hyperintensities in middle-aged adults at varying metabolic risk.

This investigation delves into the interplay between large neutral amino acids (LNAA) and metabolic syndrome (MetS) in midlife adults, examining their collective influence on brain structure. While LNAA, such as tryptophan and phenylalanine, are known to bolster cognition in youth, these relationships often reverse later in life. Our study hypothesized an earlier reversal of these benefits in middle-aged adults with MetS, potentially signaling premature brain aging. Eighty participants between 40-61 years underwent MetS component quantification, LNAA measurement via high-performance liquid chr…

Unraveling metabolic signatures in SARS-CoV-2 variant infections using multiomics analysis.

INTRODUCTION: The emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants, notably delta and omicron, has signifi … enes in lung revealed significant alterations in arginine biosynthesis, glutathione metabolism, and tryptophan metabolism upon infection with both variants and closely linked to inflammatory cytokines, indicating immune activation and oxidative stress in response to both variants. These metabolic changes were also evident in the serum, validating the presenc …

PAH deficient pathology in humanized c.1066-11G>A phenylketonuria mice.

We have generated using CRISPR/Cas9 technology a partially humanized mouse model of the neurometabolic disease phenylketonuria (PKU), carryi … reduced survival. Blood and brain phenylalanine levels are elevated, along with decreased tyrosine, tryptophan and monoamine neurotransmitter levels. They present behavioral deficits, mainly hypoactivity and diminished social interaction, locomotor deficiencies and an abnormal hind-limb clasping reflex. Changes in the morphology of glial cells, increased GFA …

Identification of differentially expressed genes and metabolism signaling pathway in the spleen of broilers supplemented with probiotic Bacillus spp.

Probiotics are essential in the body's nutrients, improving the ratio of meat to meat, immune response, and preventing diseases. In this stu … iched to metabolism signalling pathways (alpha-linolenic acid metabolism, linoleic acid metabolism, tryptophan metabolism, tyrosine metabolism, ether lipid metabolism, and metabolic pathway, etc) and immune response pathways (cytokine-cytokine receptor interaction, MAPK signalling pathway, and intestinal immune network for IgA production, neuroactive ligand- …

[Mechanism of icariin inhibiting the proliferation of human prostate cancer PC-3 cells: An exploration based on cell metabolomics].

OBJECTIVE: To study the mechanism of icariin inhibiting the proliferation of human PCa PC-3 cells based on cell metabolomics technology. ME … osphatidylcholine and lysophosphatidylethanolamine, and upregulated those of amino acid metabolites tryptophan, leucine, and proline in the PC-3 cells. CONCLUSION: Icariin inhibits the proliferation of human PCa PC-3 cells, which may be closely related to its regulatory effect on lipid metabolism (glycerophospholipid metabolism) and amino acid metabolism.

Immunometabolic Approaches Mitigating Foreign Body Response and Transcriptome Characterization of the Foreign Body Capsule.

Directing immunometabolism presents new opportunities to modulate key cell types associated with the formation of foreign body response (FBR) capsule. Contrasting approaches directing immunometabolism are investigated to mitigate FBR: a broadly suppressive metabolic inhibitor (MI) cocktail comprised of 2-deoxyglucose (2-DG), metformin, and 6-diazo-5-oxo-l-norleucine (DON) with daily systemic dosing regimen, and local weekly injection of the more narrowly focused tryptophan catabolizing IDO-Gal3 fusion protein. Treatments significantly decrease FBR capsule formed around subcutaneously implanted…

Disfunction of dorsal raphe nucleus-hippocampus serotonergic-HTR3 transmission results in anxiety phenotype of Neuroplastin 65-deficient mice.

Anxiety disorders are the most common psychiatric condition, but the etiology of anxiety disorders remains largely unclear. Our previous studies have shown that neuroplastin 65 deficiency (NP65-/-) mice exhibit abnormal social and mental behaviors and decreased expression of tryptophan hydroxylase 2 (TPH2) protein. However, whether a causal relationship between TPH2 reduction and anxiety disorders exists needs to be determined. In present study, we found that replenishment of TPH2 in dorsal raphe nucleus (DRN) enhanced 5-HT level in the hippocampus and alleviated anxiety-like behaviors. In add…

The Impact of Bisphenol A on the Anaerobic Sulfur Transformation: Promoting Sulfur Flow and Toxic H2S Production.

Bisphenol A (BPA), as a typical leachable additive from microplastics and one of the most productive bulk chemicals, is widely distributed in sediments, sewers, and wastewater treatment plants, where active sulfur cycling takes place. However, the effect of BPA on sulfur transformation, particularly toxic H2S production, has been previously overlooked. This work found that BPA at environmentally relevant levels (i.e., 50-200 mg/kg total suspended solids, TSS) promoted the release of soluble sulfur compounds and increased H2S gas production by 14.3-31.9%. The tryptophan-like proteins of microbe…