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Гут Микробиота и аутоиммунные заболевания: терапевтический потенциал пробиотики.

В последние годы все большее внимание уделяется роли кишечного микробиота в патогенезе аутоиммунных заболеваний, таких, как тип 1 диабет (T1D), ревматоидный артрит (RA) и множественный склероз (MS). Многочисленные исследования показали, что кишечный дисбиоз-несбалансированность кишечного микробного сообщества может повлиять на иммунную реакцию хост-компании путем модуляции подстрекательских путей, целостности кишечного барьера и функции иммунных клеток. Пациенты с T1D, RA и MS часто имеют меньшее количество противовоспалительных бактерий (например, Feecalibacterium prausnitzii) и слишком много

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… Additionally, Aghamohammadi et al. [ 82 ] demonstrated that administration of Saccharomyces boulardii improved antioxidant status and reduced clinical symptoms such as fatigue and pain. Another intriguing and promising, though still experimental, therapeutic strategy is FMT. …

Adult-Onset Hyperammonemia: Case Report and Review of Literature.

INTRODUCTION: Hyperammonemia is a life-threatening condition most associated with liver disease, though non-cirrhotic causes are increasingly recognized. CASE PRESENTATION: We report an 18-year-old female presenting with 1 week of progressive confusion, abdominal pain, and nausea. Initial evaluation showed normal routine laboratories but markedly elevated ammonia and respiratory alkalosis. She rapidly deteriorated, requiring intubation and intensive care. Ammonia peaked at 795 µg/dL and improved rapidly after urgent venous hemofiltration and medical therapy, with full neurological recovery by

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… Valproate inhibits mitochondrial bioenergetics and increases glycolysis in Saccharomyces cerevisiae . Sci Rep . 2020 ; 10 ( 1 ): 11785 . 32678210 10.1038/s41598-020-68725-5 PMC7367371 16. Verrotti A , Trotta D , Morgese G , Chiarelli F . Valproate-induced hyperammonemic encephalopathy . …

Runx2: a novel oncogenic effector revealed by in vivo complementation and retroviral tagging.

The Runx2 (Cbfa1, Pebp2alphaA, Aml3) gene was previously identified as a frequent target for transcriptional activation by proviral insertion in T-cell lymphomas of CD2-MYC transgenic mice. We have recently shown that over-expression of the full-length, most highly expressed Runx2 isoform in the thymus perturbs T-cell development, leads to development of spontaneous lymphomas at low frequency and is strongly synergistic with Myc. To gain further insight into the relationship of Runx2 to other lymphomagenic pathways, we tested the effect of combining the CD2-Runx2 transgene either with a Pim1 t

S1PR4-dependent Воздействие этрасимод на первичную активацию миэлоидных иммунных клеток человека.

ИСТОРИЯ ВОПРОСА: Sphingosine-1-phosphate (S1P) и его five Рецепторы S1PR1-5 играть важную роль в миграции, дифференциации и активации различных иммунных клеток. Недавно было утверждено несколько модуляторов S1PR с особой избирательностью для лечения различных подстрекательских заболеваний. Среди них Озанимод, S1PR1/5 Модулятор, утвержденный для лечения язвительного колита и множественных склерозов, и Etrasimod, S1PR1/4/5 Модулятор, утвержденный для лечения язвительного колита. Хронические самовоспалительные заболевания, такие, как вспыльчивые болезни кишечника (ББК) и язвительный колит, характ

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… Zymosan A from saccharomyces cerevisiae (1 mg/ml; Sigma-Aldrich) was dissolved in RPMI 1640 medium. 2.2 Primary human immune cell isolation and cell culture PBMCs were obtained from Buffy Coats (DRK-Blutspendedienst Baden-Württemberg-Hessen) using Pancoll (PAN Biotech) gradient centrifugation. …

Alendronate-coated long-circulating liposomes containing 99mtechnetium-ceftizoxime used to identify osteomyelitis.

Osteomyelitis is a progressive destruction of bones caused by microorganisms. Inadequate or absent treatment increases the risk of bone growth inhibition, fractures, and sepsis. Among the diagnostic techniques, functional images are the most sensitive in detecting osteomyelitis in its early stages. However, these techniques do not have adequate specificity. By contrast, radiolabeled antibiotics could improve selectivity, since they are specifically recognized by the bacteria. The incorporation of these radiopharmaceuticals in drug-delivery systems with high affinity for bones could improve the

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… In addition, sterile inflammatory foci were induced by the direct inoculation of 0.2 mL of zymosan ( Saccharomyces cerevisiae ) in sterile saline into the medullar cavity of the left tibia. …

The hemidesmosomal protein bullous pemphigoid antigen 1 and the integrin beta 4 subunit bind to ERBIN. Molecular cloning of multiple alternative splice variants of ERBIN and analysis of their tissue expression.

The bullous pemphigoid antigen 1 (eBPAG1) is a constituent of hemidesmosomes (HDs), cell-substrate adhesion complexes in stratified epithelia. Although its COOH terminus interacts with intermediate filaments, its NH(2) terminus is important for its recruitment into HDs. To identify proteins that interact with the NH(2) terminus of human eBPAG1, we performed a yeast two-hybrid screen, which uncovered a protein belonging to the LAP/LERP (for LRR and PDZ domain) protein family with 16 NH(2)-terminal leucine-rich repeats and a COOH-terminal PDZ domain. The gene for this LAP/LERP protein comprises

Targeting Cellular Senescence in Aging and Age-Related Diseases: Challenges, Considerations, and the Emerging Role of Senolytic and Senomorphic Therapies.

Cellular senescence is characterized by the permanent arrest of cell proliferation and is a response to endogenous and exogenous stress. The continuous accumulation of senescent cells (SnCs) in the body leads to the development of aging and age-related diseases (such as neurodegenerative diseases, cancer, metabolic diseases, cardiovascular diseases, and osteoarthritis). In the face of the growing challenge of aging and age-related diseases, several compounds have received widespread attention for their potential to target SnCs. As a result, senolytics (compounds that selectively eliminate SnCs

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… In addition to caloric restriction, genetic screening of long-lived mutants of Saccharomyces cerevisiae showed that the SIRT2 gene could slow down aging in this species [ 290 , 291 ]. … It has also been found that mammalian sirtuins are a family of NAD + -dependent enzymes homologous to the Saccharomyces cerevisiae gene SIRT2 [ 288 ]. To date, seven sirtuins (SIRT1 to SIRT7) have been identified in humans. …

Probiotics: Potential Benefits and Safety in Hematological Malignancies.

Cancer remains one of the most significant global health challenges, with increasing incidence and mortality rates and a substantial socioeconomic burden. Traditional cancer treatments such as chemotherapeutic drugs and radiation therapies, while effective, have significant adverse effects on the body, prompting the search for less invasive treatment options. In recent years, probiotics have emerged as a promising alternative in the prevention and treatment of cancer and its associated complications, especially in gastrointestinal malignancies. Probiotics have been found to have several benefi

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… In a systematic review of Infectious complications following probiotic ingestion, the genus Saccharomyces was the most frequent, followed by Lactobacillus, Bifidobacterium, Bacillus, Pedioccocus and Escherichi [ 72 ]. …

A case of recto-sigmoid endometriosis mimicking carcinoma.

OBJECTIVE: To evaluate the technical success, safety and outcome of endovascular coiling procedure in intracranial aneurysms. METHODS: From April 2003 to April 2009, 43 patients (23 males and 20 females), age range 11 to 70 years, mean age 46.67 +/- 11.57 years were treated for intracranial aneurysms by detachable coil deployment at Radiology Department of Aga Khan University Hospital. Aneurysm rupture with subarachnoid haemorrhage was the cause of presentation in 39 patients while 4 patients were diagnosed with un-ruptured aneurysms. At time of presentation, grading of subarachnoid haemorrha

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… Microbial strains, inoculum preparation, and establishment of OD 600 –CFU calibration curves Yeast ( Kluyveromyces marxianus and Saccharomyces bayanus ), AAB ( Komagataeibacter xylinus and Gluconobacter oxydans ), and LAB ( Leuconostoc mesenteroides ) were used in this study. …

CRISPR-Cas: Converting A Bacterial Defence Mechanism into A State-of-the-Art Genetic Manipulation Tool.

Bacteriophages are pervasive viruses that infect bacteria, relying on their genetic machinery to replicate. In order to protect themselves from this kind of invader, bacteria developed an ingenious adaptive defence system, clustered regularly interspaced short palindromic repeats (CRISPR). Researchers soon realised that a specific type of CRISPR system, CRISPR-Cas9, could be modified into a simple and efficient genetic engineering technology, with several improvements over currently used systems. This discovery set in motion a revolution in genetics, with new and improved CRISPR systems being

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… Simplified CRISPR-Cas genome editing for Saccharomyces cerevisiae. J. Microbiol. Methods. 2016;127:203–205. doi: 10.1016/j.mimet.2016.06.020. 66. Semenova E., Jore M.M., Datsenko K.A., Semenova A., Westra E.R., Wanner B., van der Oost J., Brouns S.J.J., Severinov K. … Cell cycle and genetic requirements of two pathways of nonhomologous end-joining repair of double-strand breaks in Saccharomyces cerevisiae. Mol. Cell. Biol. 1996;16:2164–2173. doi: 10.1128/MCB.16.5.2164. 69. Bennardo N., Gunn A., Cheng A., Hasty P., Stark J.M. …

VP3.15, двойной ингибитор GSK-3β/PDE7, уменьшает рост опухоли глиобластомы, изменяя микроопухоль в контексте PTEN дикой природы.

Глиобластома (ГБ) — неизлечимый рак мозга, и необходимо срочно определить эффективные методы лечения. Это может быть достигнуто либо путем идентификации новых молекул, либо путем переочистки наркотиков. Для определения терапевтического потенциала известных ингибиторов GSK-3β и/или PDE7 в GB был проведен анализ наркотиков с использованием модели глиомы Дрософила меланогастера. VP3.15, двойной ингибитор с противовоспалительными и нейрозащитными функциями при множественном склерозе, был выбран для дальнейшего изучения. VP3.15 продемонстрировал высокую антиопухоль против панели клеток человека и м

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… Briefly, the Gal4 transcriptional activator from Saccharomyces cerevisiae was expressed under the control of a specific promoter active in specific tissues or cells. …

Treatment of Fusarium Infection of the Central Nervous System: A Review of Past Cases to Guide Therapy for the Ongoing 2023 Outbreak in the United States and Mexico.

INTRODUCTION: Fusariosis of the central nervous system (CNS) is extremely uncommon. Treatment and outcome data from previously published cases may provide some guidance in light of the ongoing fungal meningitis outbreak in 2023 involving Fusarium spp. in the United States and Mexico. METHODS: We reviewed the published literature describing cases of invasive fusariosis of the (CNS) that included data on patient demographic characteristics, treatment, and outcome. RESULTS: Twenty-six cases met inclusion criteria. The mean age was 36 years, 55% involved females, 60% had underlying hematologic m

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… Katiyar SK Edlind TD Role for Fks1 in the intrinsic echinocandin resistance of Fusarium solani as evidenced by hybrid expression in Saccharomyces cerevisiae Antimicrob Agents Chemother 2009 53 1772 1778 10.1128/AAC.00020-09 19258277 PMC2681557 18. …

Urgent considerations on renal immune-related adverse events in oncology practice.

In recent years, immune checkpoint inhibitors (ICIs) have emerged as a critical component of hematological malignancies and solid malignant tumors therapy. However, clinical practice has revealed that these agents may induce immune-related adverse events (irAEs). Notably, renal irAEs stands out as a significant clinical concern, frequently necessitating treatment discontinuation and thereby enabling tumor progression. Renal irAEs constitutes a critical consideration for patients with cancer complicated by chronic kidney disease (CKD). This review systematically examines the immunologic pathoge

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… When the ICI variant was expressed in Saccharomyces boulardii, the engineered probiotic yeast effectively targeted the PD-1-PD-L1 axis and significantly reduced intestinal tumor burden in a murine model of colorectal cancer resistant to conventional ICI therapy, while simultaneously inhibiting tissue … macrophage membrane derived nanoparticles; ICAM-1, intercellular adhesion molecule-1; LFA-1, Leukocyte function-associated antigen-1; SIRPα, signal regulatory protein α; APCP, ADP[α,β-CH 2 ], AMP-CP; GC NV, Glycyrrhiza uralensis Fisch roots-derived nanovesicles; GPNP, gelase-responsive nanoparticle; Sb, Saccharomyces …

Болезнь Альцгеймера (AD) в множестве склерозов (MS): систематический обзор опубликованных случаев, механистические связи между AD и MS и возможная клиническая оценка AD в MS.

Фон: Болезнь Альцгеймера (AD) и множественный склероз (MS) являются двумя неврологическими расстройствами, которые могут создавать огромное бремя для качества жизни человека. В связи с появлением новых методов лечения, которые существенно увеличивают продолжительность жизни, возможно, наблюдается рост распространенности АД среди пожилых пациентов с НР. Цель: На основе предыдущего обзора, посвященного сосуществованию MS-AD, этот обзор был направлен не только на расширение круга поиска литературы, но и на изучение возможных механистических связей между клиническими маркерами MS и AD. Методы: мы

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… Similarly, Saccharomyces and Aspergillus were found in higher concentrations in MS patients; the former was positively correlated with circulating basophils but negatively correlated with regulatory B cells, while the latter was positively correlated with activated CD16+ dendritic cells. 120 Additionally …

Rice bran arabinoxylan compound as a natural product for cancer treatment - an evidence-based assessment of the effects and mechanisms.

ContextRice bran arabinoxylan compound (RBAC) is a natural immunomodulator with anticancer properties.ObjectiveThis study critically evaluates the available evidence on the biological pathways of RBAC and its effects on cancer treatment.MethodsThis secondary analysis of a scoping review includes studies evaluating the mechanisms of RBAC on healthy or malignant cells, animal models, or humans for cancer prevention or treatment. Data from randomized controlled trials on survival and quality of life outcomes were subjectd to meta analysis.ResultsThe evidence synthesis was based on 38 articles. RB

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… Heat-killed Saccharomyces cerevisiae (Desm.) Meyen, commonly known as baker’s or brewer’s yeast, can cause apoptosis in breast cancer cells after being engulfed by phagocytic tumour cells (Ghoneum and Gollapudi 2004 ). …

[Molecular mechanisms controlling the cell cycle: fundamental aspects and implications for oncology].

INTRODUCTION: Comprehension of cell cycle regulation mechanisms has progressed very quickly these past few years and regulators of the cell cycle have gained widespread importance in cancer. This review first summarizes major advances in the understanding of the control of cell cycle mechanisms. Examples of how this control is altered in tumoral cells are then described. CURRENT KNOWLEDGE AND KEY POINTS: The typical mammalian cell cycle consists of four distinct phases occurring in a well-defined order, each of which should be completed successfully before the next begins. Progression of euka

A novel NKG2A alpaca nanobody targeting immune checkpoint blockade for the treatment of malignant melanoma.

Alpacas belong to the Camelidae family. Antibodies produced through alpaca immunization are called nanoantibodies. Compared to traditional antibodies, nanoantibodies have several characteristics, including smaller molecular weight, stable structure, high homology with human antibodies, and suitability for prokaryotic expression. Malignant melanoma (MM) is a severe and aggressive form of cancer that affects both humans and animals. It commonly arises in the mucous membranes of the skin, nose, mouth, anus, and digestive tract, as well as in the choroid of the eyes. Multiple factors contribute to

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… Isolation of antigen specific llama VHH antibody fragments and their high level secretion by Saccharomyces cerevisiae . J Biotechnol . ( 2000 ) 78 : 11 – 21 . doi: 10.1016/S0168-1656(99)00228-X , PMID: 10702907 7. Wicklein D Otto B Suling A Elies E Lüers G Lange T . …

Screening of traditional Chinese medicine monomers as ribonucleotide reductase M2 inhibitors for tumor treatment.

BackgroundRibonucleotide reductase (RR) is a key enzyme in tumor proliferation, especially its subunit-RRM2. Although there are multiple therapeutics for tumors, they all have certain limitations. Given their advantages, traditional Chinese medicine (TCM) monomers have become an important source of anti-tumor drugs. Therefore, screening and analysis of TCM monomers with RRM2 inhibition can provide a reference for further anti-tumor drug development.AimTo screen and analyze potential anti-tumor TCM monomers with a good binding capacity to RRM2.MethodsThe Gene Expression Profiling Interactive An

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… Extracting and Measuring dNTP Pools in Saccharomyces cerevisiae. Methods Mol Biol. 2019;1999:103–127. doi: 10.1007/978-1-4939-9500-4_6. 5. Li J, Pang J, Liu Y, Zhang J, Zhang C, Shen G, Song L. …

Biological Evaluation of Naproxen-Dehydrodipeptide Conjugates with Self-Hydrogelation Capacity as Dual LOX/COX Inhibitors.

The use of peptide-drug conjugates is emerging as a powerful strategy for targeted drug delivery. Previously, we have found that peptides conjugated to a non-steroidal anti-inflammatory drug (NSAID), more specifically naproxen-dehydrodipeptide conjugates, readily form nanostructured fibrilar supramolecular hydrogels. These hydrogels were revealed as efficacious nano-carriers for drug delivery applications. Moreover, the incorporation of superparamagnetic iron oxide nanoparticles (SPIONs) rendered the hydrogels responsive to external magnetic fields, undergoing gel-to-solution phase transition

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… Docking Studies The crystal structure of Ovis aries COX-1, expressed in Spodoptera frugiperda (PDB code: 3N8Z) [ 29 ], Mus musculus COX-2, expressed in Spodoptera frugiperda (PDB code: 3NT1) [ 30 ], and Saccharomyces cerevisiae yeast 20S open gate proteasome (PDB code: 3MG8) [ 31 ] were used as the …

Alu-dependent RNA editing of GLI1 promotes malignant regeneration in multiple myeloma.

Despite novel therapies, relapse of multiple myeloma (MM) is virtually inevitable. Amplification of chromosome 1q, which harbors the inflammation-responsive RNA editase adenosine deaminase acting on RNA (ADAR)1 gene, occurs in 30-50% of MM patients and portends a poor prognosis. Since adenosine-to-inosine RNA editing has recently emerged as a driver of cancer progression, genomic amplification combined with inflammatory cytokine activation of ADAR1 could stimulate MM progression and therapeutic resistance. Here, we report that high ADAR1 RNA expression correlates with reduced patient survival

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… RNA-Seq analysis identifies a novel set of editing substrates for human ADAR2 present in Saccharomyces cerevisiae. Biochemistry. 2013;52:1–27. doi: 10.1021/bi4006539. 38. Gao J, et al. Hedgehog signaling is dispensable for adult hematopoietic stem cell function. …