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[Study on the activity of fibroblast in hypertrophic scar].

To determine the state of fibroblast during the process of development of hypertrophic scar (HS), 40 specimens of HS in different periods were collected. The expressions of prolifrating cell nuclear antigen (PCNA) and Ag-protein in nucleolar organizer regions (Ag NORs) as well as the content of total amino acids in the tissues were examined. The hypertrophic scar of 1st and 3rd month old, the expression of PCND and Ag NORs were the highest. In the 9th and 12th month old, althrough PCNA was nearly negative, but the expression of Ag NORs was low. The content of total amino acid was increased gra

Value of AgNORS in fine needle aspiration cytology of breast lesions.

Silver staining Nucleolar organizer regions (AgNORs) were studied in fine needle aspirates of 48 benign and 36 malignant lesions and correlations with histological types, menstrual status, tumor size and lymph node involvement were looked for. A semiquantitative scoring system (AgNOR Score) reflecting total AgNOR area was applied to each of these parameters and compared with the standard counting method. The objectives were to examine the pattern of distribution and discriminating capacity of AgNORs in fine needle aspirates of different breast lesions and to evaluate the AgNOR scoring system a…

Intrahepatic sarcomatous cholangiocarcinoma.

A 77-year-old man, diagnosed with a liver tumor, was referred to our hospital. Abdominal ultrasonography demonstrated a low echoic mass in t … intercellular adhesion. While there was no region containing differentiated epithelial components, silver impregnation staining revealed structures resembling regenerating bile ducts. The tumor cells were positive for wide-keratin, and for vimentin staining. Tumor cells were carcinoembryonic antigen (CEA)-positive and alpha-feto protein (AFP)-negative. …

[AgNORs staining of exfoliated cells in the nasopharynx for diagnosis of nasopharyngeal cancer].

AgNORs were investigated in exfoliated cell smears from nasopharynx and in sections of biopsied tissues of NPC patients. It showed increased tendency of AgNOR activity that was significantly higher in carcinomas than in benign tumors, chronic nasopharyngitis, and normal nasopharyngeal tissues. This suggests that AgNOR technique of exfoliated cells might be useful for NPC diagnosis, follow-up of NPC patients, and identification of precancerous lesions of the nasopharynx.

[Study on argyrophilic inclusions of multisystem atrophy (Oppenheimer)].

UNLABELLED: Non-hereditary olivo-ponto-cerebellar atrophy (OPCA) and striato-nigral degeneration (SND) have been looked upon as a single disease entity called multisystem atrophy (MSA) by Oppenheimer. This study revealed that both intracytoplasmic argyrophilic inclusions (AI) in pontine neurons and glial (argyrophilic) cytoplasmic inclusions (GCIs) widely distributed in the CNS are characteristics of MSA. MATERIALS: a) 12 cases with MSA, b) 16 cases with autosomal dominant (AD) form of spinocerebellar degeneration (SCD): AD form of OPCA 5 cases, Joseph disease 4 cases, AD-dentatorubropallidol

Болезнь Креуцфельдта-Якоба с сильным участием белого мозга и мозжечка.

Мы описываем пациента с болезнью Крецфельда-Якоба (CJD) атактического и панэнцефалопатического типа. Посмертная экспертиза выявила характерные повреждения УХД в сером веществе, и было замечено глубокое участие белого вещества в иммуноцитохимических методах. Повреждения ультраструктурных белых веществ были идентичны повреждениям, описанным в экспериментально переданном CJD. Наблюдалась заметная потеря мозжечных гранул с фактическим исчезновением параллельных волокон, но клетки Пуркинье были слегка сокращены. Электронные микроскопические исследования выявили значительные дегенеративные изменения

Совпадение в аннотации оригинала
We describe a patient with Creutzfeldt-Jakob disease (CJD) of the ataxic and panencephalopathic type. Postmortem examination revealed the ch … studies revealed extensive degenerative changes including cytoplasmic vacuoles in both cell types. Silver methods disclosed massive impregnation of white matter and striking abnormalities of Purkinje cells consisting of hypertrophy and flattening of thick dendritic branches, reduction in the number of terminal branchlets, segmentary loss of spines and p …

Neuronal alterations in experimental Creutzfeldt-Jakob disease: a Golgi study.

Neuronal alterations in the cortex of hamsters with experimental Creutzfeldt-Jakob disease (CJD) were studied utilizing the rapid Golgi and Golgi-Hortega methods. In terminally ill hamsters many pyramidal neurons showed a considerable decrease in the number of dendritic spines often with moderate irregularities in size. The shafts of apical dendrites in some cortical pyramidal neurons were tortuous. Occasional neuronal cell bodies were irregular in contour and showed hole-like empty spaces. Two types of focal swellings, which involved both axons and dendrites, were observed; focal swellings we

Determination of trace element Ag and Sc concentrations in human cataractous lenses.

For the detection and determination of the trace elements Ag and Sc in mature human cataractous lenses, the methods of neutron activation analysis was used. As regards Ag, attempts made in the past to establish its presence in the cataractous lens had failed, while Sc has not so far been thoroughly investigated. Our study of the two elements was also correlated to the age and sex of the patients with senile cataract. It was found that once senile cataract has fully matured, the determined trace-element concentrations no longer appear to be related to age and/or sex of the patients.

[Migratory capabilities of Bithynia inflata molluscs in a western Siberian opisthorchiasis focus (studied by the use of radioactive marking)].

Migratory abilities of Bithynia inflata have been studied. The investigations have been carried out in the West-Siberian nidus of opisthorchiasis during four summer seasons since 1975. Mollusks were marked with isotopes 60Co and 110m Ag. It has been established that the mollusks do not migrate for long distances, prefer to lead a settled life and do not leave their constant biotopes even during spring floods. Migrations of mollusks within their biotopes take place, in general, along the shore line. After drying of water mollusks spend this unfavourable period in surface layers of the bottom mu

[Glucosidases specific for the cyanogenic glucoside triglochinin. Purification and characterization of beta-glucosidases from Alocasia macrorrhiza Schott].

beta-Glucosidases have been isolated from Alocasia macrorrhiza plants. The enzymes are highly specific for the hydrolysis of the cyanogenic glucoside triglochinin endogenous to this plant. Upon chromatography of protein extracts on cation exchange resins and Sephadex G-200, separation into various enzymatically active bands was observed. The main fractions possess molecular weights of approximately 310000 and 105 000, as shown by preparative ultracentrifugation in a linear saccharose gradient. The beta-glucosidases are composed of subunits (molecular weight 55 000 to 60 000), as revealed by so

Physiological and genomic characterization of Methanothermobacter thermautotrophicus strain Z-73A, an isolate from deep underground.

Underground biomethanation represents a promising solution for green energy storage. An isolate of Methanothermobacter thermautotrophicus wa … l (up to 0.6 M) than to NaCl (optimal 0.04-0.4 M). The strain was also tested for its resistance to silver nanoparticles. The whole-genome sequence of the strain was determined, and the corrosion potential was evaluated. To visualise the cells, Scanning Electron Microscopy (SEM) and a method of Advanced Environmental Scanning Electron Microscopy (A-ESEM) …

Quercetin Released Biomedical Hybrid Hydrogels Fabricated by Silk Fibroin and Sodium Alginate with Incorporation of Ag@rGO Nanosheets.

The drug-encapsulated hybrid hydrogels possessed several expected properties, including porous microstructure, conductivity, adhesive strength, antioxidant activity, antibacterial properties, and cytocompatibility, and have great potential in biomedical applications, such as skin wound hydrogel dressings and bio-adhesives. In this paper, the quercetin-loaded hybrid hydrogels (SSA-QRs) were fabricated using silk fibroin (SF), alginate, and silver-doped reduced graphene oxide (Ag@rGO) nanosheets, incorporating quercetin-encapsulated PF-127 (PF127-QR) micelles. Scanning electron microscopy (SEM) …

Rapid SERS-machine learning-enabled virulence profiling of Acinetobacter baumannii for environmental surveillance.

Acinetobacter baumannii (A. baumannii) is a critical multidrug-resistant pathogen capable of environmental dissemination through water and veterinary sources. Rapid analytical methods to identify high-risk strains are essential for One Health surveillance. Here, we report a proof-of-concept surface-enhanced Raman spectroscopy and machine learning (SERS-ML) framework for rapid, label-free virulence-associated profiling of A. baumannii. Interestingly, whole-cell SERS spectra from 20 environmental and veterinary isolates (10 virulent, 10 avirulent) with silver nanoparticles (AgNPs) colloid yielde…

Advanced breast cancer detection: leveraging MIM structure containing triangular cavity and elliptical island for refractive index and temperature sensing.

In this study, a highly sensitive surface plasmon polariton (SPP)-based sensor with a metal-insulator-metal (MIM) configuration is proposed and numerically investigated for multifunctional detection, including refractive index sensing, breast cancerous cell discrimination, and temperature sensing. The sensor consists of an air-filled MIM waveguide integrated with a triangular cavity, further optimized by embedding an internal elliptical silver island to enhance light-matter interaction. Finite element method simulations reveal that the elliptical cavity design significantly improves transmissi…

Highly Conductive Ag Flakes-Based Bio-Adhesive for Multi-Functional Epidermal Electronics.

Robust skin-electronic interfaces are essential for signal fidelity of wearable bioelectronics, particularly under dynamic physiological con … biological tissues to rigid electronic components; second, it facilitates the uniform dispersion of silver flakes into mechanically stable, percolated networks that guarantee highly reliable conductivity. Exploiting these advantages, we demonstrate fully integrated epidermal devices, including in situ fabricated bioelectrodes for reliable electrocardiogr …

1,3,5-Triazine functionalized chitosan encapsulated nanocomposite for electrochemical dual detection of glucose and H2O2.

A new electrochemical sensor was designed based on a silver/iron oxide (AgFe2O3)-incorporated functionalized chitosan (AgFe2O3/FCS) nanocomposite for the detection of glucose and hydrogen peroxide (H2O2). The AgFe2O3/FCS nanocomposite was synthesized via an ultrasonic method followed by a hydrothermal technique. Its structure, chemical composition, and morphology were analyzed using several techniques, such as X-ray diffraction (XRD), Fourier transform infrared (FT-IR) spectroscopy, Raman spectroscopy, X-ray photoelectron spectroscopy (XPS), Scanning Electron Microscopy (SEM), Transmission Ele…

Computationally tailoring the adsorption behavior of ZrSe2 for SF6 decomposition species in GIS: Mechanistic insights into Cu2O/Ag2O functionalization.

Serving as an essential insulating gas, sulphur hexafluoride (SF6) is applied in gas-insulated switchgear (GIS) and exerts a key effect on insulation and arc suppression. When the electrical and mechanical breakdowns occurred, SF6 decomposition can lead to the formation of H2S, SO2, SO2F2, and SOF2. Adsorption effect of transition-metal oxides modified ZrSe2, including Cu2O-ZrSe2 and Ag2O-ZrSe2 towards sensing gases during SF6 decomposition in this study was analyzed using the first-principles simulation. The optimized structures were determined, presenting advantages over initial ZrSe2 contai

The application of machine learning-assisted serum SERS technology in the early screening and prognosis evaluation of osteoporosis.

Early detection, early intervention, early treatment, and timely prognostic monitoring of osteoporosis are crucial for improving patients' q … tra pre- and post-treatment. Through a comparative analysis of the enhancement capabilities of four silver nanoparticle types, we identified that only those synthesized via sodium citrate reduction enabled clear detection of the characteristic peaks in serum samples. Subsequently, by directly integrating the serum SERS spectra obtained via this substrate …