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Synthesis and biodistribution of [11C]fludiazepam for imaging benzodiazepine receptors.

As a tracer for in vivo studies on benzodiazepine receptors, 7-chloro-1,3-dihydro-5-(2-fluorophenyl)-1-[11C]methyl-2H-1,4- benzodiazepin-2-o … ading doses. The effect of the loading dose on the uptake was also found in the heart and lungs. By autoradiography using [11C]fludiazepam, a higher accumulation was visualized in the cortex and thalamus than in other regions.

Autoradiographic distribution of [3H]acetylcholine binding sites in the cervical spinal cord of man and some other species.

The distribution of [3H]acetylcholine ([3H]ACh) and [3H]ACh co-incubated with 1-mM nicotine (muscarinic receptor), and [3H]ACh co-incubated with 1.5 microM atropine (nicotinic receptor) binding sites were studied in man and compared to monkey, cat and rat using quantitative in vitro autoradiography. The highest density of total [3H]ACh binding sites was found in laminae II-III, IX (motor neuron areas) and X close to the central canal. The distribution pattern of the muscarinic cholinergic binding sites was similar to that of the total cholinergic binding. In general the number of nicotinic bin…

Trichomonal vaginitis: evaluation of serological tests and identification of immunoreactive surface peptides.

An indirect haemagglutination assay (IHA) with polysaccharide and protein antigens of Trichomonas vaginalis and an enzyme linked immunosorbe … the 11 sera tested by RIPA showed positive reactions with surface antigens, which were confirmed by autoradiography.

Enkephalin convertase in the gastrointestinal tract an associated organs characterized and localized with [3H]guanidinoethylmercaptosuccinic acid.

Enkephalin convertase (carboxypeptidase EH; EC 3.4.17.10), a carboxypeptidase B-like enzyme which processes hormone and neuropeptide precurs … Co+2-stimulated carboxypeptidase B activity which is not detectable in crude homogenates. In vitro autoradiography with [3H]guanidinoethylmercaptosuccinic acid localizes enkephalin convertase to the epithelial cells of the stomach, colon, and intestine, the islet cells of the pancreas, and the acinar cells of the submandibular gland. This localization contrasts …

Schistosoma mansoni: migration potential of normal and radiation attenuated parasites in naive guinea pigs.

Compressed tissue autoradiography using [75Se]selenomethionine labelled parasites has been used to investigate the migration potential of normal and radiation attenuated cercariae of Schistosoma mansoni in naive guinea pigs. By Day 14 after infection. 44% of normal parasites were detected as reduced silver foci in the liver; this value corresponded well with the number of liver parasites recovered by retrograde perfusion of the hepatic portal system on Day 42 (42% of the challenge). In contrast, cercariae subjected to 50 krad of gamma irradiation failed to migrate out of the skin. The migratio…

Increased cerebral blood flow during hypercapnia is not affected by lesion of the nucleus locus ceruleus.

To test the hypothesis that the putative noradrenergic innervation of intraparenchymal cerebral blood vessels from the nucleus locus ceruleus mediates the vasodilatory response to hypercapnia, regional cerebral blood flow was measured by iodo-[14C]antipyrine autoradiography in awake and restrained rats with unilateral 6-hydroxydopamine lesion of the nucleus locus ceruleus and in unlesioned control rats. Hypercapnia, induced by the inhalation of 5% or 8% CO2 in air for 8 minutes caused a 2 to 5-fold increase in regional cerebral blood flow. However, despite a marked reduction of about 90% in co…

Alterations in muscarinic cholinergic receptor densities induced by thiamine deficiency: autoradiographic detection of changes in high- and low-affinity agonist binding.

Animals fed a diet deficient in thiamine or treated with a drug preventing the utilization of thiamine (thiamine antagonist) exhibited alterations in ligand binding to muscarinic receptors in several brain regions. Using quantitative techniques of receptor autoradiography, an increase in muscarinic receptor binding was demonstrated in such regions as the corpus callosum, lamina VI of the parietal cortex, caudate-putamen, ventral nucleus of the thalamus, stratum lacunosum moleculare and stratum oriens of the hippocampus, and the hilus of the area dentata. As a result of thiamine deficiency, thi…

An autoradiographic study of the distribution of fibers from the dorsal motor nucleus of the vagus to the digestive tube of the rat.

At the present time, complete agreement on the origin and course of parasympathetic preganglionic fibers to the alimentary canal has not bee … The injection sites and tissue sections from various areas of the digestive tube were processed for autoradiography. A heavy label was observed in the injection site and it could be traced down the vagus nerve through the thorax into the abdomen. Labelled vagal fibers were found in the parasympathetic ganglia of the stomach, small intestine and colon.

An autoradiographic study of human gliomas: growth kinetics of anaplastic astrocytoma and glioblastoma multiforme.

When 3H-thymidine was administered either intravenously or intra-arterially to 9 patients with gliomas (5 glioblastoma multiforme, 3 anaplastic astrocytoma, and one mixed glioma) shortly before operation, and tissue obtained from the biopsy and, subsequently, at autopsy was examined by autoradiography, it was found that 2 of the glioblastomas diluted out the labelling in the two- to four-month interval between labelling and autopsy, whereas 3 other glioblastomas and the 2 anaplastic astrocytomas retained labelled neoplastic cells in parts of the tumours at autopsy after intervals of from three…

[Route of infection of the biliary tract: experimental evidence for an enterohepaticobiliary bacterial cycle (author's transl)].

Suspensions of Escherichia coli bacteria were injected into the colon of ten mongrel dogs. Primarily, the cystic duct and the distal choledo … ver and gallbladder wall, the tritiated bacteria could be seen in phagocytes and capillary ducts by autoradiography. We conclude that there is an enterohepaticobiliary bacterial cycle which could explain the pathogenesis of infections in biliary tract diseases.

Electron microscope autoradiographic studies of the erythroblasts of a case of congenital dyserythropoietic anaemia, type II.

The bone marrow cells of a patient with congenital dyserythropoietic anaemia, type II, were incubated with 3H-thymidine, 3H-uridine or 3H-leucine for 1 h and studied using the technique of electron microscope autoradiography. Several of the erythroblasts which either displayed the characteristic subsurface double membranes or showed various non-specific abnormalities of the nuclear membrane were found to be actively engaged in DNA, RNA and protein synthesis. Both members of some pairs of erythroblasts which were joined together by a spindle bridge were found to be engaged in DNA synthesis, ind…

Lymphocyte involvement in rheumatoid arthritis. Studies during thoracic duct drainage.

Grip strength, ring size, duration of morning stiffness, and the number of tender joints improved significantly in 9 patients with severe rh … fusion, some 51Cr-labeled lymphocytes could be found in the inflamed synovium and synovial fluid by autoradiography, and radioactivity was detected over the joints by surface counting of gamma radiation. Active rheumatoid arthritis recurred in all subjects at variable intervals after cessation of lymph drainage. These findings are compatible with the hypothesis t …

T-cell migration into allografts.

The ability of T and B lymphocytes to migrate into skin allografts undergoing rejection was studied in mice. Spleen cells from CBA/J mice sensitized to transplantation antigens of A/J or C57BL/6 mice were separated on immunabsorbent columns into purified populations of T and B cells, labeled in vitro with 3H-uridine and injected intravenously into CBA/J mice with 7-day old skin iso and allografts (A/J or C57BL/6). The mice were sacrificed 24 h later and studied by autoradiography. After transfer of either unfractionated spleen cells or T cells, large numbers of labeled cells were found in the …

Translocation of sugar and tritiated water in squash plants.

When (14)C-sugar and THO were simultaneously introduced through a cut side vein or flap of a squash leaf (Cucurbita melopepo, Bailey cv. torticollis) concurrent translocation of (14)C-sugars, T-photosynthates and THO with parallel, almost flat, gradients was observed in the petiole for periods of 1 to 3 hr. Parallel translocation gradients were not observed when (14)C was introduced as (14)CO(2) and T by painting a leaf with THO. Autoradiography of frozen sections to locate the tissues in which THO was moving was unsuccessful. Steam-girdling blocked the movement of (14)C and T when (14)C-gluco…

Carbon-11-labelling of the histone deacetylase 6 radioligand EKZ-001.

PURPOSE: Histone deacetylase 6 (HDAC6) is involved in microtubule stabilisation and protein degradation, and is attracting attention as a th … and administration in normal mice. Radioligand binding in the mouse brain was evaluated by in vitro autoradiography and compared with HDAC6 immunostaining. RESULTS: [11C]EKZ-001 was obtained in a radiochemical yield of 35.5 ± 4.2 % (decay corrected to [11C]CO2; n = 3), with a radiochemical purity and molar activity at the end of the synthesis of 96.0 ± 1.1 % and …

PET Study of S1PR1 Expression in Rodent Model of Myocardial Infarction.

PURPOSE: Acute myocardial infarction (MI) is a leading cause of morbidity and mortality worldwide. Sphingosine-1-phosphate (S1P) is a bioact … munofluorescence (IF) experiment was conducted to demonstrate changes in S1PR1 expression after MI. Autoradiography was performed to evaluate the distribution of [18F]FS1P1 in MI heart tissues. MI (n = 4) and sham (n = 4) mice were scanned with [18F]FS1P1 PET at 2 days and 2 weeks post-MI, radioactivity uptake in the myocardium was calculated as the percentage of …

Radiolabeled Rhein as Small-Molecule Necrosis Avid Agents for Imaging of Necrotic Myocardium.

A rapid and accurate identification of necrotic myocardium is of great importance for diagnosis, risk stratification, clinical decision-maki … n a mouse model of ethanol-induced muscular necrosis by gamma counting, histochemical staining, and autoradiography. A lead tracer for visualization of necrotic myocardium was assessed by single photon emission computed tomography/computed tomography (SPECT/CT) imaging in a rat model with reperfused myocardial infarction. The necrosis avidity mechanism of the tra …

Advancing In Vivo Detection of T-Cell Function: Development and Preclinical Evaluation of 89Zr-Ivuxolimab, a Human OX40 PET Tracer.

The variable response to cancer immunotherapies highlights a critical gap in our ability to predict and monitor treatment efficacy. To addre … ed a transgenic human OX40 murine model of acute graft-versus-host disease. Ex vivo gamma counting, autoradiography, and immunohistochemistry were performed to verify tracer-binding specificity. Results: 89Zr-ivuxolimab was reproducibly synthesized and showed significantly increased in vitro binding to activated human T cells versus resting cells (P < 0.0001) and …

Разработка и оценка [11С]DPA-813 и [18F]DPA-814: новые индикаторы PET, не чувствительные к единому нуклеотидному полиморфизму человека, Rs6971.

P ЦЕЛЬ: Протеин транслокатора 18 кДа (TSPO) — широко используемый маркер для визуализации нейроинфламмации через Томографию позитронных выбросов (PET). Однако подавляющее большинство сообщаемых индикаторов PET TSPO демонстрируют низкую связь с общей изоформой человека TSPO (rs6971; A147T), что делает их непригодными для всеобщего использования среди населения в целом. В этом исследовании мы разработали и проконтролировали два новых индикатора, предназначенных для изображения TSPO у пациентов всех генотипов.МЕТОДЫ: Новые аналоги известных лигандов TSPO были синтезированы, оценены с точки зрения

Совпадение в аннотации оригинала
PURPOSE: The translocator protein 18 kDa (TSPO) is a widely used marker for imaging neuroinflammation via Positron Emission Tomography (PET) … e (WT) or A147T TSPO) and radiolabelled with carbon-11 or fluorine-18. They were evaluated in situ (autoradiography on genotyped human brain tissue) and in vivo (rat, both WT and clinically relevant experimental autoimmune encephalomyelitis (EAE) neuroinflammation model) as potential polymorphism-insensitive TSPO PET tracers. RESULTS: Two new TSPO ligands, DPA-8 …

Development of [18F]FAMTO: A novel fluorine-18 labelled positron emission tomography (PET) radiotracer for imaging CYP11B1 and CYP11B2 enzymes in adrenal glands.

INTRODUCTION: Primary aldosteronism accounts for 6-15% of hypertension cases, the single biggest contributor to global morbidity and mortali … ia use of sulfonium salt (1) and boronic ester (2) precursors. [18F]FAMTO was evaluated in vitro by autoradiography of pig adrenal tissues and in vivo by determining its biodistribution in rodents. Rat plasma and urine were analysed to determine [18F]FAMTO metabolites. RESULTS: [18F]FAMTO is obtained from sulfonium salt (1) and boronic ester (2) precursors in 7% …