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Cardiovascular Effects, Antioxidant Activity, and Phytochemical Analysis of Rubus ulmifolius Schott Leaves.

Wild blackberry (Rubus ulmifolius Schott) is a culinary and medicinal plant traditionally used to treat various ailments, including hypertension. This study evaluated the vasorelaxant effects of five crude leaf extracts of R. ulmifolius (hexane, dichloromethane, ethyl acetate, methanol, and aqueous), as well as the hypotensive and antioxidant activities of its methanolic extract (MERu), and analyzed its phytochemical profile. Crude extracts, obtained using a Soxhlet apparatus, were tested in vitro on isolated rat aortic rings precontracted with phenylephrine. The hypotensive effect of MERu was…

The Diatom Odontella aurita Modulates Melanogenesis in B16-F0 Cell Line.

Melanin, a pigment synthesized by melanocytes, serves as the primary defense against UV-induced skin damage due to its potent antioxidant properties. There is increasing interest in natural substances capable of modulating the melanogenic pathway, particularly in hypopigmentation disorders. This study investigated the effect of methanolic extracts from the diatom Odontella aurita-authorized as a food supplement in the EU-on melanogenesis in the B16-F0 murine melanoma cell line. The research evaluated melanin content, tyrosinase activity, and the expression of melanogenesis-related genes and pr…

β-sitosterol: A novel molluscicidal agent from Liagora farinosa against Biomphalaria alexandrina snails with computational insights into its interaction with the retinoid X Receptor.

Schistosomiasis is a tropical parasitic disease that presents significant public health concerns, occurring in both acute and chronic forms. In Egypt, the freshwater snail Biomphalaria alexandrina is the intermediate host of Schistosoma mansoni. This study evaluated the molluscicidal potential of β-sitosterol, isolated from the marine red alga Liagora farinosa, along with its methanolic extract, against B. alexandrina. Bioassay results demonstrated potent molluscicidal activity, with the methanolic extract exhibiting LC50 and LC90 values of 187.5 mg/L and 231.8 mg/L, respectively, and β-sitost…

Диметил Фумарат против "Димил Фумарат". Монометил Фумарат: нерешенные фармакологические проблемы.

Диметилфумарат (ДМФ) зарекомендовал себя в качестве одного из основных методов лечения множественных склерозов и псориазов и в настоящее время изучается возможность повторного заражения многими другими заболеваниями, не вызывающими злокачественные заболевания. Несмотря на десятилетия предклинических исследований, некоторые вопросы, связанные с фармакологией, остаются нерешенными, при этом продолжается обсуждение вопроса о том, какой из метилэстеров фумарата, будь то DMF или монометилфумарат (MMF), является активным ингредиентом. Общепризнанно, что ДМФ подвергается ферментированному гидролизу M

Совпадение в аннотации оригинала
Dimethyl fumarate (DMF) has established a significant position among therapies for multiple sclerosis and psoriasis and is now being investi … the active ingredient. It is generally accepted that DMF undergoes enzymatic hydrolysis to MMF and methanol. However, there is disagreement regarding its exact site, its extent, and the responsible enzyme(s). The enzymatic mechanisms, particularly the roles of carboxylesterases-1 and 2, vary across tissues and species, complicating the translation of in v …

Computational Evaluation on the Interactions of an Opaque-Phase ABC Transporter Associated with Fluconazole Resistance in Candida albicans, by the Psidium guajava Bio-Active Compounds.

OBJECTIVES: Candida albicans is an opportunistic pathogen that occurs as harmless commensals in the intestine, urogenital tract, and skin. I … tracted and molecular characterization of Cdrp1 and Cdrp2 was done by PCR amplification. P. guajava methanolic extract was checked for the antifungal efficacy against the resistant strain of C. albicans. Further in-silico docking involves retrieval of ABC transporter protein and ligand optimization, molinspiration assessment on drug likeness, docking simul …

Eucalyptus camaldulensis for combating the Multi Drug Resistance Bacterial Strains (MDRS).

Antibiotic resistance in bacteria is the primary challenge for health worldwide. The widespread of food poisoning due to use of stored food items increasing day by day. The present study was designed to research the protective effect of Eucalyptus camaldulensis methanolic extract again selected strains of bacteria (Escherichia coli, Bacillus cereus, Bacillus subtilis and Staphylococcus aureus). The treatment was used for several concentrations and then characterized on the basis of temperature and pH variations. Results were observed by MIC (minimum inhibitory concentration) against these stra…

Antioxidant and cytotoxicity activity of a nanoemulsion from Satureja kermanica (Lamiaceae).

OBJECTIVE: Satureja kermanica is a native species with a relatively wide distribution in Iran, which has been studied less. Due to the low stability of the plants, in this study, the methanolic extract of S. kermanica (MSK) along with a nanoemulsion (NEK) preparation was evaluated in terms of antioxidant and cytotoxic activity. MATERIAL AND METHODS: The aerial parts of S. kermanica were collected and after studying the organoleptic characteristics and quality control parameters, were extracted with methanol. Total phenolic compounds and total flavonoids of the plant were measured. A nanoemuls…

Hepatoprotective and cardioprotective effect of Artemisia nilagirica leaf extract on E. coli challenged broiler chicken.

Artemisia nilagirica is an important medicinal plant found to exhibit several medicinal properties but the use of its leaves for combating E. coli infection has not been scientifically validated in poultry. The present study was conducted to evaluate the protective effects of methanol leaf extract of A. nilagirica (ANE) on E. coli challenged broiler chickens. Three hundred and thirty, day-old broiler chickens, were divided into 6 groups of 55 each, with group EX infected intraperitoneally (I/P) with LD50 dose of 1 × 107 cfu/ml of E. coli; group(s) EA1, EA2 and EA3 infected I/P with 1 × 107 cfu…

Chemical Composition, Nutritional Value, Antioxidative, and In Vivo Anti-inflammatory Activities of Opuntia Stricta Cladode.

The cactus family plant has been used in folk medicine for a long time. In this work, Opuntia stricta chemical composition and its antioxidative and anti-inflammatory properties were investigated. Our results showed that O. stricta is highly rich in fibers and minerals. The present study assessed the levels of polyphenol contents and antioxidant and in vivo anti-inflammatory activities. The highest phenolic compounds and antioxidant activity were observed in the methanolic extract. Concerning the qualitative analysis, nine phenolic and organic acids were identified and quantified by high-perfo…

Assay for the quantification of abemaciclib, its metabolites, and olaparib in human plasma by liquid chromatography-tandem mass spectrometry.

An isotope-dilution bioanalytical assay for abemaciclib and its metabolites in combination with olaparib was developed and validated in human plasma K2 EDTA. For the quantitative assay, human plasma samples (or human plasma QC samples) were spiked with internal standard solution before a simple protein precipitation with methanol. The extract was injected onto a liquid chromatography-tandem mass spectrometry (LC-MS/MS) instrument where it was chromatographically separated by a polar end-capped reversed phase column and guard using gradient elution with water and methanol both modified with 0.2…

Investigation of antioxidant, antimicrobial, and enzymatic properties of thermophilic cyanobacteria extracts.

BACKGROUND: The present study investigated the antioxidant, antimicrobial, and partial enzymatic properties of 52 thermophilic cyanobacteria isolates in vitro. MATERIALS AND METHODS: The DPPH scavenging method was applied to test the antioxidant potential of isolates' methanol extracts. Agar block diffusion and agar well diffusion methods were used to evaluate the antimicrobial activity and measured in milimeters. The measurement of enzyme activity was carried out by a modification of the agar block method by the growth of the cyanobacteria. RESULTS: Among the cyanobacterial extracts, strain…

Radiolytic degradation of 1,2,4-trichlorobenzene (TCB) in some organic solvents by gamma rays: The kinetic properties of complete dechlorination of TCB and its pathway.

This study investigates the degradation of TCB in methanol, ethanol, hexane, and benzene solutions using gamma radiolysis. Kinetic properties of TCB dechlorination and its pathway are examined, with TCB selected as a representative chlorinated organic compound. Chromatograms of irradiated samples and mass spectra of liquid-phase products are presented. The change in concentration of TCB, dichlorobenzenes (DCB), chlorobenzene (MCB), and benzene with absorbed doses are observed. The radiation-chemical yield (G values) of TCB in the solvents are calculated as 1.83, 2.56, 1.93, and 1.84 100eV-1 in…

Novel Nanostructured Pd/Co-Alumina Materials for the Catalytic Oxidation of Atmospheric Pollutants.

Cobalt-doped alumina catalysts were prepared using different methods, either conventional wet impregnation (WI) and/or advanced spray impreg … ning available Co active sites and a better Pd-Co interaction. MeOH desorption studies revealed the methanol oxidation mechanism: the Co/Al catalysts promoted the partial oxidation of MeOH to formaldehyde (HCHO) and dehydration to dimethyl ether (DME), while the Pd-based Co/Al catalysts enhanced the complete oxidation of methanol to CO2 and H2O.

Mango peel extracts and mangiferin chromatographic Fourier-transform infrared correlation with antioxidant, antidiabetic, and advanced glycation end product inhibitory potentials using in silico modeling and in vitro assays.

Mangifera indica peels are a rich source of diverse flavonoids and xanthonoids; however, generally these are discarded. Computational studies revealed that mangiferin significantly interacts with amino acid residues of transcriptional regulators 1IK3, 3TOP, and 4f5S. The methanolic extract of Langra variety of mangoes contained the least phenol concentrations (22.6 ± 0.32 mg/gGAE [gallic acid equivalent]) compared to the chloroform (214.8 ± 0.12 mg/gGAE) and ethyl acetate fractions (195.6 ± 0.14 mg/gGAE). Similarly, the methanolic extract of Sindhri variety contained lower phenol concentration…

Lepidium peruvianum as a Source of Compounds with Anticancer and Cosmetic Applications.

Lepidium peruvianum-an edible herbaceous biennial plant distributed in the Andes-has been used for centuries as food and as a natural medicine in treating hormonal disorders, as an antidepressant, and as an anti-osteoporotic agent. The presented study aims to prove its beneficial cosmetic and chemopreventive properties by testing the antiradical, whitening, cytotoxic, and anticancer properties of differently colored phenotypes that were extracted using three solvents: methanol, water, and chloroform, with the help of the chemometric approach to provide evidence on the impact of single glucosin…

Synergistic Phytochemical and Nanotechnological Exploration of Melia azedarach With Silver Nitrate: Elucidating Multifaceted Antimicrobial, Antioxidant, Antidiabetic, and Insecticidal Potentials.

Plants are a rich source of bioactive compounds with significant pharmaceutical and health applications. This study explores the phytochemical, therapeutic, and phytotoxic properties of Melia azedarach by analyzing extracts from its bark, flowers, leaves, and fruits using six solvents: ethanol, methanol, acetone, hexane, chloroform, and distilled water. Twenty-one phytochemical tests were conducted, revealing significantly positive results for various tests. However, the ethanolic and methanolic flower extracts yielded no significant results in other tests. The highest total phenolic content w…

Chiral zwitterionic stationary phases based on Cinchona alkaloids and dipeptides: Application in chiral separation of dipeptides under reversed phase conditions.

Chromatographic behavior of novel chiral stationary phases with bonded selectors based on Cinchona alkaloids modified with dipeptides was st … retention depends on the hydrophobicity of an analyte. Hydrophobic dipeptides are better eluted by methanol-rich solvents, hydrophilic dipeptides are better eluted with water-rich solvents, and dipeptides with an intermediate hydrophobicity demonstrate a U-shaped or more complicated dependence of the retention factor on the percentage of methanol. Even a …

Acute Ethanol Intoxication: Αn Overlooked Cause of High Anion Gap Metabolic Acidosis With a Marked Increase in Serum Osmolal Gap.

Measurement of serum osmolal gap is a useful tool in suspected toxic alcohol ingestion. Normal levels of osmolal gap are typically <10 mOsm/kg). Osmolal gap >20 mOsm/kg is usually caused by ingestion of methanol, ethylene glycol, isopropanol, propylene glycol, diethylene glycol, or organic solvents such as acetone but rarely of ethanol alone. Herein, we describe the case of a severe ethanol intoxication presenting with a marked increase in the osmolal gap. An 18-year-old male was referred to the emergency department of our hospital, in a comatose state, following binge drinking. blood gas anal…

Broad-class volatile organic compounds (VOCs) detection via polyaniline/zinc oxide (PANI/ZnO) composite materials as gas sensor application.

Metal-oxide doped conductive polymers have been investigated as sensors in the field of gas-sensing. Recent developments have highlighted the role of intrinsically conductive polymers, that have reportedly offered high surface response towards the detection of volatile organic compounds (VOCs). In this work, we optimize the development of gas-sensors made of Polyaniline/Zinc oxide (PANI/ZnO) composite, capable of detecting a varied class of VOCs such as, ammonia, acetone, formaldehyde, methanol, and ethanol. The conductivity of these sensors is evaluated at room temperature and are investigate…

Potential chemopreventive effects of Broccoli extract supplementation against 7, 12 dimethyl Benz(a)anthracene (DMBA) -induced toxicity in female rats.

Dietary components have recently received rapidly expanding attention for their potential to halt or reverse the development of many oxidative stress-mediated diseases after exposure to environmental toxicants. 7, 12 dimethylbenz(a)anthracene (DMBA) is one of the most common environmental pollutants. The present study aimed to evaluate the chemo-preventive effects of broccoli as a nutritional component against DMBA intoxication in rats. A daily dose of aqueous (1 ml/rat) and methanolic (150 mg/kg) broccoli extracts, respectively, was given to 50-day-old female rats for 26 successive weeks afte…