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Synthesis of phosphorylated hyper-cross-linked polymers and their efficient uranium adsorption in water.

Uranium (U) is hazardous and radioactive, wastewater containing U(VI) should be treated before being discharged. Here, two novel uranium adsorbents, phosphorylated hyper-cross-linked bisphenol A (PHCP-1) and fluorene-9-bisphenol (PHCP-2) were separately synthesized via Friedel-Crafts reaction followed by phosphorylation using phosphorus oxychloride. PHCPs had a BET surface area (up to 564 m2/g) with pore sizes of 2.2-2.8 nm. These adsorbents were used for the first time for uranium adsorption from water and demonstrated outstanding adsorption performance. PHCP-2 had a great uranium adsorption

Importance of Zebrafish as an Efficient Research Model for the Screening of Novel Therapeutics in Neurological Disorders.

Over the previous years, the use of an animal model has become very common for the screening of novel drugs. Animal model represents the complex problems of humans into the simplest forms, so these can be extended further to be included in the experimental procedure. The most successful models in neuroscience, rats and mice, are undoubtedly considered as one of the best models to understand the psychology of the mammalian brain and its associated functions involved in behavioral repertoire. Moreover, recently researchers in behavioral neuroscience are focusing more on the use of aquatic animal

NKG2D-CAR-transduced natural killer cells efficiently target multiple myeloma.

CAR-T-cell therapy against MM currently shows promising results, but usually with serious toxicities. CAR-NK cells may exert less toxicity when redirected against resistant myeloma cells. CARs can be designed through the use of receptors, such as NKG2D, which recognizes a wide range of ligands to provide broad target specificity. Here, we test this approach by analyzing the antitumor activity of activated and expanded NK cells (NKAE) and CD45RA- T cells from MM patients that were engineered to express an NKG2D-based CAR. NKAE cells were cultured with irradiated Clone9.mbIL21 cells. Then, cells

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… Then, CAR-NKAE cells suffered only a minimal loss of NKG2D expression at 6 days post-transduction (transduction efficiency 20.65% ± 5.95). CD45RA − memory T cells showed higher transduction efficiency (52.68% ± 12.2) at 5 days post-transduction. … Efficient and robust NK-cell transduction with baboon envelope pseudotyped lentivector. Front Immunol. 2019;10:2873. doi: 10.3389/fimmu.2019.02873. 49. Bari R, Granzin M, Tsang KS, Roy A, Krueger W, Orentas R, et al. …

Minute Cu2+ coupling with HCO3- for efficient degradation of acetaminophen via H2O2 activation.

Homogeneous Cu2+-mediated activation of H2O2 has been widely applied for the removal of organic contaminants, but fairly high dosage of Cu2+ is generally required and may cause secondary pollution. In the present study, minute Cu2+ (2.5 μM) catalyzed H2O2 exhibited excellent efficiency in degradation of organic pollutants with the assistant of naturally occurring level HCO3- (1 mM). In a typical case, acetaminophen (ACE) was completely eliminated within 10 min which followed the pseudo-first-order kinetics. Singlet oxygen and superoxide radical rather than traditionally identified hydroxyl rad…

An efficient and combined placental T1 -ADC acquisition in pregnancies with and without pre-eclampsia.

PURPOSE: To provide a new approach to jointly assess microstructural and molecular properties of the human placenta in vivo fast and efficiently and to present initial evidence in cohorts of healthy pregnancies and those affected by pre-eclampsia. METHODS: Slice and diffusion preparation shuffling, built on the previously proposed ZEBRA method, is presented as a robust and fast way to obtain T1 and apparent diffusivity coefficient (ADC) values. Joint modeling and evaluation is performed on a cohort of healthy and pre-eclamptic participants at 3T. RESULTS: The datasets show the ability to obt

Efficient Conversion of Levulinic Acid to Methyl Levulinate Over SO2-₄/SnO₂ Nanocatalysts.

The present work focus on the synthesis of x% SO2-₄ loaded SnO₂ nanocatalyst at different percentages (x = 2.3, 4.6, 6.9, 9.2, and 11.5% w/w) for acid-catalyzed esterification. The synthesized catalysts were characterized by X-ray powder diffraction (XRD), fourier transform infrared spectroscopy (FT-IR), brunauer-emmett-teller (BET), scanning electron microscopy (SEM), transmission electron microscope (TEM), temperature-programmed desorption, and thermal gravimetric analysis (TGA/DTA) techniques. Methyl levulinate is an important chemical feedstock and its production through a catalytic route

Human Coronaviruses Do Not Transfer Efficiently between Surfaces in the Absence of Organic Materials.

Human coronaviruses, including SARS-CoV-2, are known to spread mainly via close contact and respiratory droplets. However, other potential means of transmission may be present. Fomite-mediated transmission occurs when viruses are deposited onto a surface and then transfer to a subsequent individual. Surfaces can become contaminated directly from respiratory droplets or from a contaminated hand. Due to mask mandates in many countries around the world, the former is less likely. Hands can become contaminated if respiratory droplets are deposited on them (i.e., coughing or sneezing) or through co

Collagen has a unique SEC24 preference for efficient export from the endoplasmic reticulum.

SEC24 is mainly involved in cargo sorting during COPII vesicle assembly. There are four SEC24 paralogs (A-D) in vertebrates, which are classified into two subgroups (SEC24A/B and SEC24C/D). Pathological mutations in SEC24D cause osteogenesis imperfecta with craniofacial dysplasia in humans. sec24d mutant fish also recapitulate the phenotypes. Consistent with the skeletal phenotypes, the secretion of collagen was severely defective in mutant fish, emphasizing the importance of SEC24D in collagen secretion. However, SEC24D patient-derived fibroblasts show only a mild secretion phenotype, suggest

Chlorpromazine Efficiently Treats the Crisis of Pheochromocytoma: Four Case Reports and Literature Review.

Pheochromocytoma multisystem crisis (PMC) is a potentially lethal emergency due to catecholamine secretion. The condition manifests as severe hypertension to intractable cardiogenic shock and has a high mortality rate. This study explored the efficacy and safety of applying chlorpromazine on PMC patients. The study included seven patients (median age, 42 years; range, 14-57 years) diagnosed with pheochromocytoma. Four consecutive PMC patients were admitted to our critical care unit between 2016 and 2020 due to abdominal or waist pain, nausea, and vomiting. Their blood pressure (BP) fluctuated

Electrospun Cellulosic Membranes toward Efficient Chiral Resolutions via Enantioselective Permeation.

Cellulose tris(3,5-dimethylphenylcarbamate) (CDMPC), known as one of the most versatile chiral selectors packed in columns for chiral chromatography, is electrospun for the first time. The electrospun nanofibers with a mean diameter of 329 nm form a self-standing nonwoven textile with a specific surface area of 5.6 m2/g. The textile is sandwiched between commercially available polytetrafluoroethylene membrane filters as a support material to fabricate a CDMPC membrane system for the chiral resolution of a racemic mixture, (R,S)-1-(1-naphthyl)ethanol. A vacuum filtration of the racemic mixture

Efficient purification of hydrogen cyanide by synergistic effects of electrochemical and liquid phase catalysis.

In this study, Co, Cu, Pd, and Pd/Cu composite metal ions were used to synthesize metal nanoparticles with high efficiency in purifying hydrogen cyanide gas. The pure liquid phase catalytic purification of hydrogen cyanide gas was studied. According to the removal rate, the Pd/Cu composite metal ions had the best purification efficiency among the nanoparticles of different metal types. The removal rate order was Pd/Cu>Pd>Cu>Co. The gas after reaction were analyzed by gas chromatography, and it was found that HCN was converted into CO2, N2 and NH3 by nanoparticles. Then, Pd/Cu composite metal i…

Fabrication and Characterization of Graphene Oxide-Coated Plate for Efficient Culture of Stem Cells.

BACKGROUND: For stem cell applications in regenerative medicine, it is very important to produce high-quality stem cells in large quantities in a short time period. Recently, many studies have shown big potential of graphene oxide as a biocompatible substance to enhance cell growth. We investigated if graphene oxide-coated culture plate can promote production efficiency of stem cells. METHODS: Three types of graphene oxide were used for this study. They are highly concentrated graphene oxide solution, single-layer graphene oxide solution, and ultra-highly concentrated single-layer graphene ox…

LPAQR-Net: Efficient Vertebra Segmentation From Biplanar Whole-Spine Radiographs.

Vertebra segmentation from biplanar whole-spine radiographs is highly demanded in the quantitative assessment of scoliosis and the resultant sagittal deformities. However, automatic vertebra segmentation from the radiographs is extremely challenging due to the low contrast, blended boundaries, and superimposition of many layers, especially in the sagittal plane. To alleviate these problems, we propose a lightweight pyramid attention quick refinement network (LPAQR-Net) for efficient and accurate vertebra segmentation. The LPAQR-Net consists of three components: (1) a lightweight backbone netwo

Is axillary botulinum toxin efficient in controlling secondary Raynaud's phenomenon? A case report.

Raynaud's phenomenon when secondary to underlying systemic disease such as systemic sclerosis occurs early in the disease course and progression can bring significant morbidity such as pain, digital ulceration, and necrosis. Standard medical therapies are aimed at promoting distal arterial vasodilation but are often inadequate in managing Raynaud's phenomenon. Options for refractory cases include surgical and chemical sympathectomy with Botulinum neurotoxin type A (BoNT/A) hand injections but the latter can be associated with transient hand weakness. We describe the case of a 35-year-old woman

Efficient Messenger RNA Delivery to the Kidney Using Renal Pelvis Injection in Mice.

Renal dysfunction is often associated with the inflammatory cascade, leading to non-reversible nephrofibrosis. Gene therapy has the ability to treat the pathology. However, the difficulty in introducing genes into the kidney, via either viral vectors or plasmid DNA (pDNA), has hampered its extensive clinical use. Messenger RNA (mRNA) therapeutics has recently attracted attention as alternative gene therapies. mRNA allows protein production into post-mitotic cells without the need for transport to the nuclei in the target cells. However, few studies have reported the delivery of mRNA to the kid

Efficient and accurate identification of ear diseases using an ensemble deep learning model.

Early detection and appropriate medical treatment are of great use for ear disease. However, a new diagnostic strategy is necessary for the absence of experts and relatively low diagnostic accuracy, in which deep learning plays an important role. This paper puts forward a mechanic learning model which uses abundant otoscope image data gained in clinical cases to achieve an automatic diagnosis of ear diseases in real time. A total of 20,542 endoscopic images were employed to train nine common deep convolution neural networks. According to the characteristics of the eardrum and external auditory

Characterization of M116.1p, a Murine Cytomegalovirus Protein Required for Efficient Infection of Mononuclear Phagocytes.

Broad tissue tropism of cytomegaloviruses (CMVs) is facilitated by different glycoprotein entry complexes, which are conserved between human CMV (HCMV) and murine CMV (MCMV). Among the wide array of cell types susceptible to the infection, mononuclear phagocytes (MNPs) play a unique role in the pathogenesis of the infection as they contribute both to the virus spread and immune control. CMVs have dedicated numerous genes for the efficient infection and evasion of macrophages and dendritic cells. In this study, we have characterized the properties and function of M116, a previously poorly descr

Ebola Virus Requires Phosphatidylserine Scrambling Activity for Efficient Budding and Optimal Infectivity.

Ebola virus (EBOV) attaches to target cells using two categories of cell surface receptors: C-type lectins and phosphatidylserine (PS) receptors. PS receptors typically bind to apoptotic cell membrane PS and orchestrate the uptake and clearance of apoptotic debris. Many enveloped viruses also contain exposed PS and can therefore exploit these receptors for cell entry. Viral infection can induce PS externalization in host cells, resulting in increased outer PS levels on budding virions. Scramblase enzymes carry out cellular PS externalization; thus, we targeted these proteins in order to manipu

Optimizing Active Tumor Targeting Biocompatible Polymers for Efficient Systemic Delivery of Adenovirus.

Adenovirus (Ad) has risen to be a promising alternative to conventional cancer therapy. However, systemic delivery of Ad, which is necessary … P5N2LG-FA and P5N5LG-FA (Ad/P5N2LG-FA and Ad/P5N5LG-FA, respectively) exerted superior transduction efficiency compared to naked Ad or Ad complexed with P5N5LG (Ad/P5N5LG) in folate receptor (FR)-overexpressing cancer cells (KB and MCF7). All three nanocomplexes (Ad/P5N5LG, Ad/P5N2LG-FA, and Ad/P5N5LG-FA) internalized into cancer cells through coxsackie aden …