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Graphene oxide-reinforced thermosensitive hydrogel co-delivering paclitaxel and dexamethasone for restenosis prevention.

Restenosis remains a significant limitation of vascular intervention procedures, primarily driven by the proliferation and inflammation of human-derived vascular smooth muscle cells (hVSMCs). This study developed a thermosensitive chitosan/β-glycerophosphate (CS-β-GP) hydrogel reinforced with graphene oxide (GO) for the localized co-delivery of paclitaxel (PTX) and dexamethasone (DEX). The incorporation of GO increased the storage modulus from approximately 3.95 kPa to 6.96 kPa, enhancing mechanical strength and accelerating gelation near body temperature (~36°C-37°C). SEM analysis revealed a …

Development and evaluation of Alkanna tinctoria root extract and mupirocin nanoparticles-loaded porous films for the burn wound management.

This study aimed to fabricate and evaluate chitosan-polymethacrylic acid polyelectrolyte complex (CSPMA-PEC) porous films incorporating nanoparticles of Mupirocin and Alkanna tinctoria root extract for the management of burn wound. ATR-FTIR and differential scanning calorimetry (DSC) confirmed formulation component compatibility and thermal stability up to 150 °C. The CSPMA-PEC films exhibited a highly porous, interconnected structure (mean pore size 66 μm and 71.2% porosity), controlled biodegradation (42.3%) over 30 days, and suitable mechanical properties for handling and application (tensi…

Biomass-Derived Conductive Hydrogel-Based Electronic-Skin Patch for Integrated Wearable Bioelectronics and Real-Time Wound-Status Monitoring and Treatment.

Conductive hydrogel-based electronic-skin (e-skin) patches represent an emerging category of smart wound dressings, facilitating innovative … patch. It is fabricated via supramolecular assembly of carboxymethyl cellulose (CMC), carboxymethyl chitosan (CMCS), and poly(vinyl alcohol) (PVA). The resulting network, strengthened by hydrogen bonding, π-π interactions, and covalent cross-linking, is mechanically robust, conductive, and intelligently responsive. The e-skin patch exhibited outstanding me …

Development of zein-based nanoparticles for oral co-delivery of insulin and liraglutide and preliminary in vivo evaluation in Danio rerio.

Oral delivery of peptide-based therapeutics remains a major challenge due to enzymatic degradation and poor intestinal permeability. This study reports the development of hybrid zein-Eudragit RS100-chitosan nanoparticles for the co-delivery of insulin and liraglutide (Z-ERS-CS/I-LIRA), aiming to protect both peptides and support their oral administration. The nanoparticles were prepared by nanoprecipitation and exhibited suitable physicochemical properties, including nanoscale size (~230 nm), low polydispersity (PDI < 0.1), and positive zeta potential (~ + 46 mV). Structural and thermal analys…

Functionalized Thermosensitive Hydrogel Regulates the Immune Microenvironment and Stem Cell Differentiation to Enhance Cartilage Regeneration.

Osteoarthritis (OA) is a heterogeneous degenerative joint disease involving cartilage degradation, subchondral bone remodeling, and chronic inflammation, which collectively lead to pain and disability. Current therapeutic strategies remain insufficient to effectively halt disease progression. To address this, we engineered a multifunctional injectable hydrogel based on hydroxybutyl chitosan (HBC) coloaded with strontium acetate (Sr) and nanosized chondroprogenitor cell-derived exosomes (CPC-EXOs) (HBC@Sr@CPC-EXOs) to modulate the inflammatory joint microenvironment and drive cartilage regenera…

Mucoadhesive and corneal permeable nano eye drops for improved anti-glaucoma therapy.

Glaucoma, characterized by elevated intraocular pressure (IOP), is the leading cause of irreversible vision loss worldwide. While topical eye drops remain the standard for IOP control, ocular surface dynamic and static barriers significantly limit intraocular drug bioavailability. Enhancing corneal retention and permeability is therefore crucial for effective glaucoma therapy. Here, we developed lignin-based nanoparticles coated with quaternary ammonium chitosan oligosaccharide (Lig-QCOS) for encapsulating and delivering the lipophilic drug latanoprost (Lat), a first-line hypotensive agent. Th…

Polysaccharide-functionalized metal-polyphenol network-coated porous microspheres for improving the treatment of probiotics in ulcerative colitis.

Probiotic therapy offers a new approach to the treatment of ulcerative colitis (UC), but oral drug delivery still faces challenges such as l … henol network (MPN) coating, and then layer-by-layer electrostatic deposition of an ethylene glycol chitosan/ hyaluronate (GCS/HA) polysaccharide gel layers via electrostatic interactions. The porous microspheres provide efficient probiotic loading and physical protection; the MPN coating confers transferrin-responsive degradability, enabling on-demand rel …

Seleno-chitooligosaccharide-induced modulation of intestinal barrier function: Role of inflammatory cytokines, tight junction proteins, and gut microbiota in mice.

This study aimed to explore the function of Seleno-chitooligosaccharide (SOA) on the intestinal barrier through regulation of inflammatory cytokines, tight junction protein, and gut microbiota in mice. The results of ELISA assay demonstrated that SOA significantly increased the levels of IL-2, IL-10, and IFN-γ in serum and ileum. Meanwhile, SOA increased the levels of IL-4 in the ileum (p < 0.05). In addition, Diamine Oxidase (DAO) concentration was decreased in ileum by SOA treatments (p < 0.05). The administration of SOA significantly upregulated the expression of ZO-1 and Occludin in the il

Tracking chemical interactions of caspofungin on silver nanoparticles through SERS spectroscopy for antifungal applications.

Silver nanoparticles (AgNP) act as efficient drug delivery carriers due to particular surface chemistry and high surface area, and even though such may present toxicity in function of the size distribution and molecular ion coverage, have been receiving special attention for their inhibitory potential against bacteria and fungi. This study explored the use of AgNP to deliver the antifungal caspofungin (CFG) for in vitro combating Candida albicans. CFG molecules were coadsorbed with low-weight chitosan (LWC) on AgNP surfaces, in the presence and absence of 2-mercaptoethanol (ME) surface modifie…

A Self-Assembled Nanoreactor for Realizing Antibacterial Photodynamic/Gas Therapy and Promoting Wound Healing.

Among various treatments employed to solve the global problem of bacterial infection, photodynamic therapy (PDT) is recognized as a method with great potential to inactivate a wide range of bacteria without the development of drug resistance. However, many commonly used photosensitizers (PSs) have the disadvantages of poor water-solubility and potential toxicity, which limits their clinical application. Additionally, nitric oxide (NO) has unique advantages in antibacterial treatments due to its small molecular weight. Herein, protoporphyrin IX (PpIX), L-arginine (L-Arg), and glycol chitosan (G…

Ameliorating effects of antibiotic alternatives on the performance and pathological parameters of Salmonella Typhimurium infected broiler chickens.

The purpose of this study was to investigate the effects of thyme oil (TO), chitosan nanoparticles (CS-NPs), and TO-loaded-CS-NPs on controlling Salmonella Typhimurium (S. Typhimurium) infection in broiler chickens when compared to ciprofloxacin (Cip) antibiotic treatment. The CS-NPs and TO-loaded-CS-NPs were initially characterized using a transmission electron microscope. Two hundred and forty broiler chicks were divided into six equal groups. Group 1 was given TO (1ml/4L of the drinking water). However, group 2 and group 3 were given CS-NPs and TO-loaded-CS-NPs in a 2 ml/chick dose by oral …

Наноэмульсии на основе карвакрола, загруженные диметилфумаратом, предназначены для передачи мозга через нос для лечения множественных склерозов.

Диметилфумарат (ДМП) является пероральным лекарством первой линии для лечения множественных склерозов (МС), что, к сожалению, связано с несколькими неблагоприятными явлениями, в основном затрагивающими желудочно-кишечный тракт. Доставка внутриутробных наркотиков может потенциально смягчить эти неблагоприятные последствия, повышающие терапевтическую эффективность. Цель этого исследования состоит в разработке наноэмульсия (NE) с нефтью в воде (o/w), инкапсулирующая DMF, растворенная в углеводородном (CV) существенном нефтяном компоненте нейрозащиты, для возможного синергического терапевтического

Совпадение в аннотации оригинала
Dimethyl fumarate (DMF) is a first-line oral medication for the treatment of multiple sclerosis (MS), unfortunately associated with several … rol (CV), a neuroprotective essential oil component, for a possibly synergistic therapeutic effect. Chitosan oleate as amphiphilic polymer has been selected as a surfactant, owing to its mucoadhesive and permeation enhancement properties. Spectrophotometric (FT-IR) and thermogravimetric analyses used to characterize the DMF-CV combination, revealed an incr …

Antimicrobial activity and nanoremediation of heavy metals using biosynthesized CS/GO/ZnO nanocomposite by Bacillus subtilis ATCC 6633 alone or immobilized in a macroporous cryogel.

BACKGROUND: The world society is still suffering greatly from waterborne infections, with developing countries bearing most of the morbidity … th. RESULTS: The present study highlighted a simple and cost-effective approach to biosynthesize a chitosan/graphene oxide/zinc oxide nanocomposite (CS/GO/ZnO) alone and immobilized in a macroporous cryogel as a new antimicrobial agent. Bacillus subtilis ATCC 6633 was used as a safe and efficient bio-nano-factory during biosynthesis. The formation of CS/G …

Combination application of Pickering emulsion and BLS protein nanoparticles enhances vaccine efficacy.

The deficiency of recombinant protein immune response could be compensated for by using nanoparticle platforms or adding immune enhancers, however existing vaccines or adjuvants struggle to elicit durable cellular immune responses. In this work, a protein nanoscaffold, lumazine synthase isolated from Brucella (BLS) was optimally designed that could facilitate cellular uptake of displayed antigens and the maturation of bone marrow-derived dendritic cells (BMDCs), and enhancing humoral immune responses. To enhance cellular immune response, chitosan hydrochloride-stabilized Pickering emulsion (CH…

Prevention of adhesions post-abdominal surgery: Assessing the safety and efficacy of Chitogel with Deferiprone in a rat model.

INTRODUCTION: Adhesions are often considered to be an inevitable consequence of abdominal and pelvic surgery, jeopardizing the medium and long-term success of these procedures. Numerous strategies have been tested to reduce adhesion formation, however, to date, no surgical or medical therapeutic approaches have been successful in its prevention. This study demonstrates the safety and efficacy of Chitogel with Deferiprone and/or antibacterial Gallium Protoporphyrin in different concentrations in preventing adhesion formation after abdominal surgery. MATERIALS AND METHODS: 112 adult (8-10 week

Mast cells improve functional recovery of transected peripheral nerve: A novel preliminary study.

BACKGROUND: Employment of regenerative properties of cells at the service of nerve repair has been initiated during recent decades. Effects of local transplantation of bone marrow-derived mast cells on peripheral nerve regeneration were studied using a rat sciatic nerve transection model. MATERIALS AND METHODS: A 10-mm sciatic nerve defect was bridged using a conduit chitosan-based hybrid conduit filled with BMMCs in BMMC group. In positive control group (Pos), the conduit was filled with phosphate-buffered saline alone. The regenerated nerve fibers were studied within 12 weeks after surgery.…

Osteoblast mineralization with composite nanofibrous substrate for bone tissue regeneration.

Several studies are currently ongoing to construct synthetic bone-like materials with composites of natural and polymeric materials with HA (hydroxyapatite). The present study aims to fabricate composite nanofibrous substrate of Chit/HA (chitosan/HA - 80:25) prepared by dissolving in TFA/DCM (trifluoroacetic acid/dichloromethane) (70:30, w/w) for 5 days and electrospun to fabricate a scaffold for bone tissue engineering. HA (25 wt %) was sonicated for 30 min to obtain a homogenous dispersion of nanoparticles within the Chit (80 wt %) matrix for fabricating composite nanofibrous scaffold (Chit/…

Synthesis and antifungal activity of novel s-substituted 6-fluoro-4-alkyl(aryl)thioquinazoline derivatives.

6-Fluoro-4-quinazolinol is prepared by the cyclization reaction of 2-amino-5-fluorobenzoic acid and formamide. The resulting thiol obtained by treatment of hydroxyl group with phosphorus (V) sulfide is converted under phase transfer condition to 4-substituted 4-alkylthio-6-fluoroquinazoline derivatives by reaction with halide. The structures of the compounds are confirmed by elemental analysis, IR, and (1)H NMR. Title compounds 3a, 3g, and 3h are found to possess good antifungal activities. Using the mycelial growth rate method in the laboratory, the mechanism of action of 3g against Fusarium

Shelf-Life Extension of Steam-Cooked Harpiosquillid Mantis Shrimp Meat Using High Voltage Cold Plasma and Chitooligosaccharide-Catechin Conjugate During Refrigerated Storage.

The impact of high voltage cold plasma (HVCP) and modified atmosphere packaging (MAP) combined with chitooligosaccharide-catechin conjugate (COS-CAT) on extending the shelf-life of steam-cooked Harpiosquillid mantis shrimp (SC-HMS) meat during refrigerated storage at 4°C was examined. SC-HMS samples were treated with COS-CAT at 600 ppm and packed under MAP (CO2:N2:O2 = 60:30:10) without and with the exposure to HVCP for 2.5 min (CP2.5-600) and 5 min (CP5-600). Air-packaged (AP) SC-HMS was spoiled before 9 days. However, the CP2.5-600 sample had significantly retarded growth of mesophilic bacte

Zwitterionic Powder-Based Gradient Adhesion-Lubrication Hydrogels for Efficient Hemostasis and Antiadhesion.

Uncontrolled hemorrhage, particularly from deep, irregular, and noncompressible wounds, remains a critical challenge in emergency medicine. Conventional hemostatic agents are frequently limited by poor sprayability, inadequate conformability to complex wound geometries, insufficient mechanical robustness, and lack of asymmetric adhesion properties. To address these limitations, we engineered a novel powder (CS-PSBMA@OHA) composed of zwitterionic poly(sulfobetaine methacrylate)-grafted chitosan (CS-PSBMA) and oxidized hyaluronic acid (OHA). Upon contact with moist tissue, the powder undergoes r…