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Sourdough bread enriched with exopolysaccharides and gazpacho by-products modulates in vitro the microbiota dysbiosis.

Sourdough bread enriched with soluble fiber (by in-situ exopolysaccharides production) and insoluble fiber (by gazpacho by-products addition) showed prebiotic effects an in vitro dynamic colonic fermentation performance with obese volunteer's microbiota. Bifidobacterium population was maintained whereas Lactobacillus increased throughout the colonic sections. Conversely, Enterobacteriaceae and Clostridium groups clearly decreased. Specific bacteria associated with beneficial effects increased in the ascending colon (Lactobacillus fermentum, Lactobacillus paracasei, Bifidobacterium longum and B…

Antibiotic Susceptibility Testing and Establishment of Tentative Species-Specific Microbiological Cut-off Values for Bifidobacteria Isolated from Chinese Population.

Bifidobacteria are commonly used as probiotics in the food industry. The resistance of Bifidobacterium species to antibiotics is closely linked to food safety. However, we still lack a system for the safety evaluation of antibiotic resistance in bifidobacteria, and genus-level microbiological cut-off values remain in use for the determination of phenotypic resistance of Bifidobacterium strains to a given antibiotic. Here, we collected a total of 422 gut-derived bifidobacterial strains isolated from Chinese population and identified their phenotypic resistance profiles against ampicillin, amoxi…

The gut microbiota alleviates depression by remodeling gut-brain energy metabolism.

Although peripheral-brain crosstalk regulates energy metabolism, its role in depression remains unclear. Here, we used metabolic profiling to reveal elevated fecal creatine alongside reduced plasma and cerebrospinal fluid creatine in both patients with depression and mouse depression models. Exogenous creatine produced antidepressant-like effects mediated by gut microbiota. Bifidobacterium pseudolongum was identified as a significantly reduced gut bacterial species in depression, correlating with impaired creatine absorption. Subsequent supplementation with Bifidobacterium enhanced the antidep…

Bacterial specificity of the gut microbiome predicts bone density in primary hyperparathyroidism.

Primary hyperparathyroidism causes mild-to-severe bone loss, but the reason for this heterogeneity is unclear. We investigated the role of the microbiome in 50 primary hyperparathyroidism patients. Microbiome transfers from primary hyperparathyroidism patients with and without osteoporosis to germ-free mice replicated the human bone phenotype and regulated TNF+ T cells and Th17 cells in mice. Accordingly, circulating TNF+ T cells and Th17 cells and TNF/IL17 production predicted bone density in primary hyperparathyroidism patients. Bifidobacterium longum, TNF+ T cells, and Th17 cells were media…

Ascosphaera apis as a target for the antifungal activity of symbiotic Bifidobacteria in honey bees.

INTRODUCTION: The genus Bifidobacterium is a key component of the honey bee gut microbiota, playing a fundamental role in maintaining host health and colony well-being. Alongside other core genera such as Bombilactobacillus, Gilliamella, Lactobacillus, and Snodgrassella, Bifidobacterium contributes to essential functions including nutrient digestion, immune modulation, and protection against pathogens. Among threats to honey bee health, Chalkbrood disease, caused by fungus Ascosphaera apis, remains a major concern due to detrimental effects on colony strength and honey yield. MATERIALS AND ME…

Study on the relationship between microbial composition within obstructive biliary stents and the severity of obstruction and duration of stent placement.

Biliary stent occlusion is due, in part, to biofilm formation by bacteria. However, previous culture-based approaches may not have revealed … tetes were much more abundant in occluded stents compared with non-occluded stents. An abundance of Bifidobacterium spp. and OTU00006 Bifidobacterium animalis (100%) correlated with stent occlusion severity (rho, 0.62; p<0.001; and 0.42; p = 0.03, respectively), and this relationship remained after adjusting for the duration of stent placement (p = 0.03 and 0.05, …

Changes of Intestinal Flora and the Effect on Intestinal Function in Infants With Ventricular Septal Defect After Cardiopulmonary Bypass Surgery.

This study aimed to investigate the changes in intestinal flora in infants with ventricular septal defect (VSD) after cardiopulmonary bypass (CPB) surgery and their potential relationship with postoperative gastrointestinal function recovery. Fecal samples of 20 infants with VSD were collected before and after CPB surgery at our hospital from September 2021 to March 2022. 16S rRNA was used to detect and analyze the fecal samples. The most abundant intestinal microbes in the preoperative intestinal flora were Enterococcus (37.14%), Bifidobacterium (20.71%), Shigella (8.15%), Streptococcus (5.19…

Multistrain probiotic preparation significantly reduces symptoms of irritable bowel syndrome in a double-blind placebo-controlled study.

AIM: To investigate the effect of bifid triple viable capsule, a multistrain probiotic preparation on symptoms of irritable bowel syndrome (IBS), and the amount of fecal Bifidobacterium spp. and Lactobacillus spp. of IBS patients before and after treatment. METHODS: A total of 60 IBS patients who met Rome III criteria were included in this double-blind, randomized, and placebo-controlled study. The patients were randomly assigned to receive composite probiotics or placebo for four weeks. The IBS symptoms of participants were surveyed using a questionnaire, and the amount of fecal Bifidobacter…

Effects of probiotic modulation on bone health in estrogen-deficient female Sprague-Dawley rats.

Postmenopausal osteoporosis is a common metabolic disorder caused mainly by estrogen deficiency, which accelerates bone resorption, suppresses bone formation, and increases fracture risk in aging women. Emerging evidence suggests that the gut microbiota influences skeletal homeostasis through the gut-bone axis, indicating that probiotic supplementation may provide a novel nutritional strategy for bone protection. This study evaluated the osteoprotective effects of two probiotic strains, Bifidobacterium breve i1088 and Bifidobacterium longum subsp. longum i772, in an ovariectomized Sprague-Dawl…

FMT reduces systemic inflammatory response in severe acute pancreatitis by increasing the abundance of intestinal Bifidobacteria and fecal bacteria.

Severe acute pancreatitis (SAP) is one of the leading causes of hospital admissions for gastrointestinal diseases, with a rising incidence w … P < 0.05). Fecal microbiota sequencing revealed that FMT significantly upregulated the abundance of Bifidobacterium longum among all SAP patients (P < 0.05). Receiver operating characteristic (ROC) curve analysis indicated that Bifidobacterium longum might play a critical role in the efficacy of FMT, with an area under the curve (AUC) value of 0.84. Additionally, …

Efficacy of probiotics and trimebutine maleate for abemaciclib-induced diarrhea: A randomized, open-label phase II trial (MERMAID, WJOG11318B).

BACKGROUND: Abemaciclib-induced diarrhea (AID) impairs quality of life (QOL) and treatment adherence in patients with breast cancer. Supportive treatment with loperamide is associated with constipation. We hypothesized that probiotics and trimebutine maleate (TM) would decrease the frequency of AID without causing constipation. METHODS: Hormone receptor-positive, human epidermal growth factor 2-negative advanced breast cancer patients were randomized into the probiotic Bifidobacterium (A) or probiotic Bifidobacterium and TM (B) groups. Endocrine therapy, Abemaciclib and probiotic Bifidobacter…

Molecular Characterization of Probiotics and Their Influence on Children with Autism Spectrum Disorder.

Children with autism spectrum disorder (ASD) are usually unable to express abdominal discomfort properly, and thus gastrointestinal symptoms … me minced cooked yellow vegetables in adequate ratios fortified with the studied probiotic strains (Bifidobacterium spp. and Lactobacillus spp.). Bifidobacterium strains were assessed from stool samples of children with ASD. Bifidobacterium strains were analyzed in the stools of ASD children. Recruited ASD patients received 10 g of the nutritional supplement once …

Effects of lactic acid bacteria isolated from dairy products on lipid pattern of rats fed with a high fat diet.

BACKGROUND AND OBJECTIVES: A unique characteristic of probiotics is obesity and fatty liver control. In this study, the effect of lactic-acid-bacteria (LABs) isolated from dairy products was investigated on weight changes, blood biochemical indexes and liver tissue in mice fed a high-fat diet. MATERIALS AND METHODS: A total of 49 rats were assigned to 7 groups. The LAB-treated groups received the high-cholesterol diet supplemented with Lactobacillus reuteri, Lactobacillus plantarum, and Bifidobacterium animalis isolated from yogurt. At the end of 4 weeks, body weight changes, lipid factors an…

Удельное микробное соотношение в кишечном микробиоме связано с множественным склерозом.

Гут микробиота дисбиоз связан с множественным склерозом (MS), однако причинно-следственная связь между конкретными кишечными бактериями и патогенезом MS остается слабой. Поэтому мы описали микробиом стула людей с MS (PwMS) и здоровым управлением (HC) с использованием метагеномической последовательности дробовика. PwMS продемонстрировал отличный микробиом по сравнению с HC, при этом виды Crevotella copri (PC) и Blautia были движущими силами микробных сообществ в HC и PwMS, соответственно. В результате применения к мышам видов MS-ding Blautia (Blautia Wexlerae; BW) повысились уровни кишечных мар

Совпадение в аннотации оригинала
Gut microbiota dysbiosis is associated with multiple sclerosis (MS), but the causal relationship between specific gut bacteria and MS pathog … g experimental autoimmune encephalomyelitis (EAE), an animal model of MS, we identified a lower gut Bifidobacterium to Akkermansia ratio as a hallmark of the disease. BW-administered mice also showed a lower Bifidobacterium to Akkermansia ratio pre-EAE induction which correlated with increased disease severity post-EAE induction. The importance of the Bifidobacte …

Breast milk dominant phyla and probiotic bacteria in the obese lactating women compared with normal weights.

The main purpose was to determine the abundance of dominant phyla, Bifidobacterium spp., and Lactobacillus in breast milk of obese mothers versus normal-weights in fourth month of lactation in Iranian population. Sixty health women at the fourth month of breastfeeding, aged 18-40 years, were included and categorized based on body mass index (BMI) to the obese (BMI ≥ 30 kg/m2) and normal-weights (18.5 ≤ BMI ≤ 24.9). Bacterial DNA was extracted and qPCR of the 16S region was performed after human milk donation in a sterile condition. A multiple linear mixed model was used to determine the effect…

The potential of Bacillus and Enterococcus probiotic strains to combat helicobacter pylori attachment to the biotic and abiotic surfaces.

The prevention of biofilm formation plays a pivotal role in managing Helicobacter pylori inside the body and the environment. This study showed in vitro potentials of two recently isolated probiotic strains, Bacillus sp. 1630F and Enterococcus sp. 7C37, to form biofilm and combat H. pylori attachment to the abiotic and biotic surfaces. Lactobacillus casei and Bifidobacterium bifidum were used as the reference probiotics. The biofilm rates were the highest in the solid-liquid interface for Lactobacillus and Bifidobacterium and the air-liquid interface for Bacillus and Enterococcus. The highest …

Study on the regulatory effects and mechanisms of action of bifidobacterial exopolysaccharides on anaphylaxes in mice.

This study used bifidobacterial exopolysaccharides (EPSs) from the selected strains of Bifidobacterium bifidum WBBI01 and WBIN03, Bifidobacterium breve WBBR04, Bifidobacterium infantis WBAN07 and Bifidobacterium longum WBLO01 to explore the EPSs regulatory effect on anaphylaxis in mice. First of all, allergy mouse models were established via subcutaneous injection followed by OVA gavage, and then the EPSs from the five Bifidobacteria were fed into the mice via continuous gavage. Samples were taken from the mice periodically to determine the changes of cytokine levels in serum, including those …

Biosynthesis and health promoting traits of green synthesized cobalt oxide nanoparticles.

Nanomedical applications have increased significantly. This work aimed to fabricate and characterize cobalt oxide nanoparticles (CoOnps) syn … l effect against Listeria, Staphylococcus aureus and Streptococcus as gram +ve pathogenic bacteria, Bifidobacterium bifidum 2203, Bifidobacterium bifidum LMG 10,645, Bifidobacterium breve LMC 017, Bifidobacterium angulatum 2238 and Bifidobacterium longum ATCC 15,707 as probiotics, E. coli as gram -ve bacterial model and yeast strain Candida albicans. CoOnps showe …

Multistrain probiotic rescinds quinpirole-induced obsessive-compulsive disorder phenotypes by reshaping of microbiota gut-brain axis in rats.

Obsessive-compulsive disorder (OCD) is a disabling mental condition that poses recurring bothersome intrusive thoughts, obsessions, and compulsions. Considering the positive impact of probiotics on neuropsychiatric disorders, herein, we investigated the effect of multistrain probiotic (Bifidobacterium lactis UBBLa-70, Bacillus coagulans Unique IS-2, Lactobacillus rhamnosus UBLR-58, Lactobacillus plantarum UBLP-40, Bifidobacterium infantis UBBI-01, Bifidobacterium breve UBBr-01, and glutamine) in the management of OCD-like phenotype in rats. Rats injected with quinpirole for 5 weeks showed an i…

Minocycline and Its Impact on Microbial Dysbiosis in the Skin and Gastrointestinal Tract of Acne Patients.

BACKGROUND: Associations between acne and gastrointestinal comorbidities suggest that microbial dysbiosis and intestinal permeability may pr … ontrols was depleted in probiotics Lactobacillus iners (p=0.001), Lactobacillus zeae (p=0.001), and Bifidobacterium animalis (p=0.026). After antibiotics, the gut microbiota of acne patients was depleted in Lactobacillus salivarius (p=0.001), Bifidobacterium adolescentis (p=0.002), Bifidobacterium pseudolongum (p=0.010), and Bifidobacterium breve (p=0.042), while …