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Role of angiogenesis in adenomyosis-associated abnormal uterine bleeding and subfertility: a systematic review.

BACKGROUND: Adenomyosis commonly occurs with abnormal uterine bleeding (AUB) and is associated with subfertility and a higher miscarriage ra … trol endometrium. The vascular and pro-angiogenic markers α-smooth muscle actin, endoglin, S100A13, vimentin, matrix metalloproteinases (MMPs), nuclear factor (NF)-kB, tissue factor (TF), DJ-1, phosphorylated mammalian target of rapamycin, activin A, folli- and myostatin, CD41, SLIT, roundabout 1 (ROBO1), cyclooxygenase-2, lysophosphatidic acid (LPA) 1,4-5 …

Protective role of transforming growth factor-Β3 (TGF-Β3) in the formation of radiation-induced capsular contracture around a breast implant: In vivo experimental study.

Postmastectomy radiotherapy causes capsular contracture due to fibroproliferation of the capsular tissue around the implant. In fibrosis, un … es for histological (capsule thickness, cell density), immunohistochemical, and biochemical (α-SMA, vimentin, collagen type I and type III, TGF-β1 and TGF-β3: expression level/protein level) examinations. It was determined that the levels of TGF-β1 and TGF-β3 collagen type III, which decreased as a result of radiotherapy, were brought to the control level …

HLF/HIF-2alpha is a key factor in retinopathy of prematurity in association with erythropoietin.

An HLF (HIF-1alpha-like factor)/HIF-2alpha-knockout mouse is embryonic lethal, preventing investigation of HLF function in adult mice. To investigate the role of HLF in adult pathological angiogenesis, we generated HLF-knockdown (HLF(kd/kd)) mice by inserting a neomycin gene sandwiched between two loxP sequences into exon 1 of the HLF gene. HLF(kd/kd) mice expressing 80-20% reduction, depending on the tissue, in wild-type HLF mRNA were fertile and apparently normal. Hyperoxia-normoxia treatment, used as a murine model of retinopathy of prematurity (ROP), induced neovascularization in wild-type

Inoperable Renal Malignant Glomus Tumor, the answers for all the "W's"?

Glomus tumor, arising from glomus bodies (specialized neurovascular structures involved in thermoregulation), commonly occurs in extremities and rarely in viscera. The spectrum of glomus tumors range from benign tumors to tumors with uncertain malignant potential to tumors of the malignant subtype. A vast majority of visceral glomus tumors are benign. Most common visceral tumors arise in the gastrointestinal tract. Glomus tumors of the kidney are a rare entity of which malignant glomus tumors are exceedingly rare. The index patients in the existing case reports were middle-aged males. We repor

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… Immunohistochemistry was positive for vimentin, smooth muscle actin (SMA), keratin, caldesmon and weakly positive for calponin, with 40% Ki 67 positivity, suggesting a diagnosis of malignant glomus tumor ( Figure 2D ,F). … , MSA | Radical nephrectomy, IVC thrombectomy | NA 6 | Kapogiannis (2021) (11) | 67 Male | 2.5 cm | Incidental | Right kidney lower pole | SMA, CD57, vimentin | Partial nephrectomy | NED at 15 months 7 | Nwanze (2021) (14) | 32 Male | 5.2 cm | Incidental | Right kidney lower pole | CD34, vimentin, SMA …

[Proliferation effect of ligamentum flavum cells induced by transforming growth factor β 1 and its effect on connective tissue growth factor].

OBJECTIVE: To investigate the effect of transforming growth factor β 1 (TGF-β 1) induced proliferation of ligamentum flavum cells and ligame … showed typical phenotype of ligamentum flavum fibroblasts; all cells expressed collagen type Ⅰ and vimentin, and some cells expressed collagen type Ⅲ; MTT identification showed that with the prolongation of culture time, the absorbance ( A) value of each generation of cells increased gradually, and the A value of the same generation of cells at each time …

Peroxisomal localization in the developing mouse cerebellum: implications for neuronal abnormalities related to deficiencies in peroxisomes.

In subjects with Zellweger syndrome, the most severe phenotype of peroxisomal biogenesis disorder, brain abnormalities include cortical dysplasia, neuronal heterotopia, and dysmyelination. To clarify the relationship between the lack of peroxisomes and neuronal abnormalities, we investigated peroxisomal localization in the mouse cerebellum, using double immunofluorescent staining for peroxisomal proteins. On immunostaining for peroxisomal matrix protein, while there are few peroxisomes in Purkinje cells, many locate in astroglia, especially soma of Bergmann glia. Clusters of peroxisomes were s

Ginsenoside Rb1 From the Functional Food Panax ginseng Suppresses Pancreatic Cancer Progression via Macrophage Reprogramming Through the PI3K-AKT Pathway.

Pancreatic ductal adenocarcinoma (PDAC) is a highly malignant gastrointestinal tumor, and its nutritional intervention strategies remain rel … r growth, reduced Ki67-positive proliferation, decreased liver metastatic burden, lowered Zeb-1 and Vimentin expression, and prolonged survival without obvious toxicity. GRB1 treatment was associated with dose-dependent reductions in PI3K and AKT phosphorylation and reduced M2-like macrophage polarization, as shown by decreased CD206, CD163, ARG1, CD115, a …

The activation of IL-8 receptors in cultured guinea pig Müller glial cells is modified by signals from retinal pigment epithelium.

Interleukin 8 (IL-8, CXCL8) is a pro-inflammatory chemokine which attracts neutrophils to sites of inflammation via an activation of the G-protein-coupled receptors, CXCR1 and CXCR2. However, both IL-8 and IL-8 receptors are widely expressed in various tissues and cell types, and have been suggested to be involved in other functions such as angiogenesis, tumor growth, or brain pathology. We examined the expression of IL-8 and IL-8 receptors in highly enriched primary cultures of guinea pig Muller glial cells. Immunoreactivity for CXCL8, CXCR1 and CXCR2 was observed in all cultured Muller cells

Mediterranean Diet Food Components as Possible Adjuvant Therapies to Counteract Breast and Prostate Cancer Progression to Bone Metastasis.

Bone metastasis is a serious and often lethal complication of particularly frequent carcinomas, such as breast and prostate cancers, which not only reduces survival but also worsens the patients' quality of life. Therefore, it is important to find new and/or additional therapeutic possibilities that can counteract the colonization of bone tissue. High adherence to the Mediterranean diet (MD) is effective in the prevention of cancer and improves cancer patients' health, thus, here, we considered its impact on bone metastasis. We highlighted some molecular events relevant for the development of

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… Morphological changes in the cells occur during EMT: cells acquire a spindle shape by decreasing the expression of cell adhesion molecules (i.e., E-cadherin) and by increasing the expression of mesenchymal markers (i.e., N-cadherin, fibronectin, and vimentin). … First, in MCF-7 breast cancer cells treated with I3C and ICZ , E-cadherin mRNA expression was increased while vimentin mRNA expression was attenuated. …

Corroborating evidence for platelet-induced epithelial-mesenchymal transition and fibroblast-to-myofibroblast transdifferentiation in the development of adenomyosis.

STUDY QUESTION: Do platelets play any role in the development of adenomyosis? SUMMARY ANSWER: As in endometriosis, adenomyotic lesions show … d Smad3 (both P-values <0.001 versus control). In addition, E-cadherin staining was decreased while vimentin staining in adenomyotic epithelial cells was increased, along with increased staining of proliferating cell nuclear antigen, vascular endothelial growth factor and CD31 (all P-values <0.001), markers of proliferation and angiogenesis. Staining for α …

Advanced Diagnostic Techniques

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… Cytokeratins, vimentin, CD45, and S-100 provide the starting point to help distinguish several carcinomas, sarcomas, neural tumors, and hematopoietic neoplasms from each other. … Some carcinomas frequently express vimentin, particularly endometrial carcinoma, renal cell carcinoma, salivary gland carcinoma, spindle cell carcinoma, and thyroid follicular carcinoma. …

Sertoli cell tumors of the ovary: a clinicopathologic and immunohistochemical study of 54 cases.

Ovarian Sertoli cell tumors are rare, and their morphologic spectrum, behavior, and factors influencing the latter are not clearly establish … all the tumors. Inhibin was positive in 18 of 22 tumors, calretinin in 10 of 20, CD99 in 19 of 22, vimentin in 17 of 18, smooth muscle actin in 4 of 18, neuron specific enolase in 8 of 16, S-100 in 2 of 20, and chromogranin was negative in all 21 cases studied. Although Sertoli cell tumors usually have a distinctive tubular pattern that facilitates the di …

SETDB1 induces lenalidomide resistance in multiple myeloma cells via epithelial‑mesenchymal transition and PI3K/AKT pathway activation.

SET domain bifurcated histone lysine methyltransferase 1 (SETDB1) is a histone H3K9 methyltransferase that stimulates cell proliferation by methylating AKT, which contributes to drug resistance in multiple myeloma (MM). Lenalidomide is an immunomodulatory agent widely used in the treatment of MM. However, lenalidomide resistance occurs in patients with MM. Currently, the role of SETDB1 in lenalidomide resistance in MM remains unclear. Thus, the present study aimed to explore the functional association between SETDB1 and lenalidomide resistance in MM. The analysis of GEO datasets revealed that

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… EMT is a phase in cancer metastasis that is characterized by increased expression of mesenchymal markers, such as N-cadherin and vimentin ( 32 ). Therefore, to examine the effect of SETDB1 on MM invasion, western blot analysis was used to assess the levels of N-cadherin and vimentin. … Western blot analysis revealed that LY294002 significantly decreased the levels of N-cadherin and vimentin and phosphorylation of PI3K and AKT, however, SETDB1 overexpression partially offset the effects of LY294002 ( Fig. 5D ). …

Molecular and Clinical Insights in Malignant Brenner Tumor of the Testis With Liver Metastases:A Case Report.

Malignant Brenner Tumor (mBT) is extremely rare. Although BT are almost exclusive ovarian neoplasms, they may constitute a highly unusual tumor of the testis; in fact, only seven fully documented cases have been reported to date. Because of their rarity, the pathogenesis of these tumors has not been clarified and there is no standard therapeutic approach. We report the first case of epididymal mBT with synchronous, multiple, liver metastases and a very dramatic clinical course. Both primary tumor and metastasis were subjected to mutational analysis of 20 cancer associated genes. Primary tumor

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… IHC analysis was performed for CKAE1/AE3, CK7 CK5, P63, CK20, EMA, Vimentine, Calretinine, TTF1 and PSA. Four 10-micrometer paraffin sections were used for gene profile assessment. … Furthermore, the IHC for CK20, CDx2, TTF1, CD10 and Vimentin showed a negative result, thus excluding metastases from gastrointestinal, lung, thyroid, renal cancer as well as sarcoma, respectively. …

Sarcoidosis and autoimmunity: In the depth of a complex relationship.

Sarcoidosis is a chronic granulomatous disease that can virtually affect any organ. Its etiology is unknown, although it has been proposed that environmental or biological agents can act as triggers, ultimately leading to chronic inflammation in genetically predisposed individuals. The main component of sarcoid inflammation is represented by an exaggerated T- lymphocytic cellular response to a putative antigen that could not be efficiently cleared in the patient. However, several clinical and immunological observations, such as the association of sarcoidosis to autoimmune diseases or the prese

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… Then, they analyzed the effects of exposition to vimentin in pre-immunized mice to vimentin itself and in non-immunized controls mice: lung histopathology examination showed that granulomas in vimentin-immunized mice were significantly larger than those in controls ( p < 0.0001). … In parallel, the putative pathogenetic role of vimentin, a protein involved in several cellular functions, has also been investigated ( 26 , 28 , 29 ): despite vimentin appearing to be capable of reproducing a typical sarcoidosis inflammation in an animal model, autoantibodies against vimentin can not …

TrkB promotes laryngeal cancer metastasis via activation PI3K/AKT pathway.

ObjectivesThe aim of our study was to investigate the role of TrkB pathway in tumor occurrence and development for in order to provide theoretical basis to laryngeal cancer therapy.Materials and methodsBiological characteristics of the cells were studied by migration tests and colony forming assay. Gene and protein expression analysis was performed by RT-PCR or western blot. in vivo experiments were conducted in syngeneic BALB/c mice.ResultsSignificant changes in protein and gene expression, including higher expression level of TrkB, were found in cells and laryngeal cancer specimens. we demon

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… Figure 5 TrkB induces EMT via up-regulation of Twist-1 and Twist-2 expression (A) Western blot analysis of the expression of E-cadherin, N-cadherin, and Vimentin proteins in Hep-2-TrkB cells. (B) Analysis of E-cadherin, N-cadherin, and Vimentin expression. … (D) Analysis of E-cadherin, N-cadherin, and Vimentin expression. Error bars represent standard deviations. * P <0.05 Vs control group, respectively. …

N-cadherin identifies human endometrial epithelial progenitor cells by in vitro stem cell assays.

STUDY QUESTION: Is there a specific surface marker that identifies human endometrial epithelial progenitor cells with adult stem cell activi … uiescent (Ki-67-) in the basalis and in Post-M endometrial glands and co-localized with EMT markers vimentin and E-cadherin. LARGE SCALE DATA: The raw and processed data files from the gene microarray have been deposited in the National Center for Biotechnology Information Gene Expression Omnibus data set with accession number GSE35221. LIMITATIONS, REAS …

Advanced Diagnostic Techniques

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… Cytokeratins, vimentin, CD45, and S-100 provide the starting point to help distinguish several carcinomas, sarcomas, neural tumors, and hematopoietic neoplasms from each other. … Some carcinomas frequently express vimentin, particularly endometrial carcinoma, renal cell carcinoma, salivary gland carcinoma, spindle cell carcinoma, and thyroid follicular carcinoma. …

Long non-coding RNA LINC00968 attenuates drug resistance of breast cancer cells through inhibiting the Wnt2/β-catenin signaling pathway by regulating WNT2.

BackgroundBreast cancer is one the most common cancers, making it the second leading cause of cancer-related death among women. Long non-coding RNAs (lncRNAs), with tightly regulated expression patterns, also serve as tumor suppressor during tumorigenesis. The present study aimed to elucidate the role of LINC00968 in breast cancer via WNT2-mediated Wnt2/β-catenin signaling pathway.MethodsBreast cancer chip GSE26910 was utilized to identify differential expression in LINC00968 and WNT2. The possible relationship among LINC00968, transcriptional repressor HEY and WNT2 was analyzed and then verif

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… /p-β-catenin, GSK3β/p-GSK3β and Vimentin/p-Vimentin in MCF-7/ADM cells after plasmid delivery of each group as determined by Western blot analysis. c histogram of levels of β-catenin/p-β-catenin, GSK3β/p-GSK3β and Vimentin/p-Vimentin in each group. d protein bands of LINC00968 in MCF-7/ADM cells after … Vimentin/p-Vimentin in KPL-4/ADM cells after plasmid delivery of each group as determined by Western blot analysis. h histogram of levels of β-catenin/p-β-catenin, GSK3β/p-GSK3β and Vimentin/p-Vimentin in each group. i protein bands of LINC00968 in KPL-4/ADM cells after plasmid delivery of each group …

m6A mRNA methylation initiated by METTL3 directly promotes YAP translation and increases YAP activity by regulating the MALAT1-miR-1914-3p-YAP axis to induce NSCLC drug resistance and metastasis.

BackgroundMETTL3 is an RNA methyltransferase that mediates m6A modification and is implicated in mRNA biogenesis, decay, and translation. However, the biomechanism through which METTL3 regulates MALAT1-miR-1914-3p-YAP axis activity to induce NSCLC drug resistance and metastasis is not very clear.MethodsThe expression of mRNA was analyzed by qPCR assays. Protein levels were analyzed by western blotting and immunofluorescent staining. Cellular proliferation was detected by CCK8 assays. Cell migration and invasion were analyzed by wound healing and transwell assays, respectively. Promoter activit

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… Furthermore, we performed RT-PCR, WB, and qPCR assays to confirm that co-treatment with METTL3 and YTHDF3 or siYTHDF3 regulates the expression of E-cadherin and vimentin. … Vimentin protein levels were further increased upon the co-transfection of METTL3 and YTHDF3 compared to those detected upon co-transfection with METTL3 and siYTHDF3 . …