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Stabilized nanocarriers for plasmids based upon cross-linked poly(ethylene imine).

Stabilized PEI/DNA polyplexes were generated by cross-linking PEI with biodegradable disulfide bonds. The reaction conversion of different PEIs with the amine reactive cross-linker dithiobis(succinimidyl propionate) (DSP) was investigated, and the molecular weight of the reaction products was identified. Light scattering and microelectrophoresis were employed to assess size and zeta potential of the resulting polyplexes. Polyplex morphology and mechanic stability were investigated using atomic force microscopy. Finally, albumin and erythrocyte interactions and stability against polyanions and

Lysosome-targeted ROS-responsive graphene oxide-based drug delivery system to overcome tumor DOX resistance.

The lysosomes of drug-resistant tumor cells transport doxorubicin (DOX) and its nanocarriers into lysosomes through a sequestration mechanism, making it difficult for DOX to reach the therapeutic concentration. In this study, a nanodrug delivery system (Dp/DGPP) capable of overcoming tumor lysosomal resistance was developed. The system consists of three parts: graphene oxide (GO)-Se-Se-DOX, polyethyleneimine-pluronic F127 (PEI-PF127) for improved biocompatibility, and Dp44mT for amplifying reactive oxygen species (ROS) and disrupting lysosomes. After entering MCF-7/ADR tumor cells, loading Dp4…

A Diamine Oxidase from Glutamicibacter halophytocola for the Degradation of Histamine and Tyramine in Foods.

A novel diamine oxidase (DAO) was discovered in the bacterium Glutamicibacter halophytocola (DAO-GH). The gene of DAO-GH was integrated into the genome of the yeast Komagataella phaffii and recombinantly produced under control of the methanol-inducible AOX1 promoter in a bioreactor cultivation. A high DAO activity of 70.2 ± 5.2 µkat/Lculture (5.25 ± 0.22 µkat/gprotein) was yielded after 90 h of cultivation. The DAO-GH was partially purified by the polyethyleneimine precipitation of nucleic acids, fractionated ammonium sulfate precipitation and hydrophobic interaction chromatography, resulting …

Synthesis, characterization and application of chitosan functionalized and functional graphene oxide membranes for desalination of water by pervaporation.

The use of graphene sheets in water treatment is increasing due to its adsorption capacity, reactivity, catalytic action and surface area. T … e treatment of surface of polyamide membrane. Layer-by-layer (LbL) deposition of positively charged polyethyleneimine (PEI) and negatively charged graphene oxide (GO) was followed. The PEI/GO LbL membrane's pure water flux was twice as high as compare to the original membrane. The synthesized membrane was tested against the aqueous solutions containing Na2SO4, MgSO …

Hybrid silica material as a mixed-mode sorbent for solid-phase extraction of hydrophobic and hydrophilic illegal additives from food samples.

In the current study, a novel kind of mixed-mode hydrophobic/hydrophilic hybrid silica material has been developed for the solid-phase extraction of hydrophobic and hydrophilic illegal additives from food samples. A hydrophobic hybrid silica material was first prepared by the sol-gel method with tetraethoxysilane, 3-aminopropyltriethoxysilane, and dimethyloctadecyl-[3-(trimethoxysilyl)propyl] ammonium chloride (DTSACl) as precursors. Subsequently, the hydrophobic hybrid silica material was activated by glutaraldehyde, followed by the covalent immobilization of polyethyleneimine (PEI). PEI and …

Application of nano remediation of mine polluted in acid mine drainage water using machine learning model.

Acid mine drainage (AMD) is the term used to describe drainage from coal mines with high sulfur-bearing rocks. The oxidative weathering of metal sulfides leads to AMD. The acidic environment corrodes more harmful compounds in the soil, which is spread throughout the working area. One such significant metal is copper, which is extracted in massive quantities from ores rich in sulfide. A copper-extraction resin might be created by combining diatomaceous earth (DE) particles with polyethyleneimine (PEI), which is shown to have great selectivity and affinity for copper. In this effort, PEI-DE part…

Development of an Intranasal Gel for the Delivery of a Broadly Acting Subunit Influenza Vaccine.

Influenza virus is a major cause of death on a global scale. Seasonal vaccines have been developed to combat influenza; however, they are no … mote a strong mucosal response. It consisted of a Schiff base-crosslinked hydrogel between branched polyethyleneimine and oxidized dextran. Following a prime-boost-boost schedule, an intranasal gel containing cGAMP and model antigen ovalbumin (OVA) led to the faster generation of serum IgG, IgG1, and IgG2c and significantly greater serum IgG1 levels on day 42 compa …

Recent advances on therapeutic potentials of gold and silver nanobiomaterials for human viral diseases.

Viral diseases are prominent among the widely spread infections threatening human well-being. Real-life clinical successes of the few availa … IV, NPAuG3-S8 for HSV, AgNPs of Moringa oleifera and Bruguiera cylindrica for dengue while AgNPs of polyethyleneimine and siRNA analogues displayed potency against enterovirus. The highlighted candidates are recommended for further translational studies towards antiviral therapeutic designs.

A simplified approach for efficient isolation of functional microglial cells: Application for modeling neuroinflammatory responses in vitro.

Purified microglial cells in culture are frequently used to model brain inflammatory responses but obtaining large yields of these cells on a routine basis can be quite challenging. Here, we demonstrate that it is possible to achieve high-yield isolation of pure microglial (MAC-1(+) /Fcrls(+) /Ccr2(-) ) cells from postnatal brain tissue through a simple culture procedure that mainly relies on the adhesion preference of these cells to the polycation polyethyleneimine (PEI) in serum-supplemented DMEM medium. Accordingly, other synthetic or biological substrates failed to mimic PEI effects under …

Dual-Responsive Nanoplatform: In Situ Self-Assembly Enables OFF-to-ON Photothermal Switching for Multimodal Synergistic Tumor Therapy.

Photothermal therapy has attracted extensive attention in tumor treatment owing to its spatiotemporal controllability and noninvasiveness. However, the nonselective "always on" nature of traditional photothermal nanoagents together with the intrinsic thermal resistance within tumor cells has hampered the therapeutic efficacy. Herein, we developed a tumor-microenvironment-responsive transformable prenanotherapeutic platform with a polyethyleneimine (PEI)-coated tannic acid-Ca2+ chelate core and an outer shell of Fe2+-doped ZIF-90, which enabled the in situ generation of photothermal agents, syn…

Anisotropic microgels for spatiotemporal co-delivery of anticancer drugs.

Codelivery of chemotherapeutic agents is often challenged by physicochemical incompatibility between co-administered drugs and the inability of conventional systems to achieve spatiotemporal control over drug release. Herein, anisotropic microgels were fabricated by an electrohydrodynamic co-jetting (EHDC) technique using PLGA as a sustained-release matrix and polyethyleneimine (PEI), along with a crosslinker, 1,2,3,4-butanetetracarboxylic dianhydride (BTDA), as a pH-responsive compartment, potentially enabling the compartmentalization of physicochemically incompatible drugs within a single pl…

Hyperbranched polycation-based hierarchical assembles as vaccine carrier for cascaded antigen delivery and enhanced immunity response.

Tumor vaccines have emerged as a promising strategy in cancer immunotherapy, and their therapeutic efficacy hinges on successive activation … ine) (HPAP) to form HPAP/OVA, followed by the assembly with the charge-reversal Polyethylene glycol-polyethyleneimine-2, 3-dimethylmaleic anhydride(mPEG-PEI-DMMA,PPD) via electrostatic interactions. Herein, the polyethylene glycol (PEG) shell of PPD@HPAP/OVA facilitates lymph node drainage, while its charge-reversal PPD enables pH-responsive lysosomal escape. Follo …

Electroconductive Carbon Dot/CNT-Reinforced Nanocomposite Hydrogels for Sequential Dual-Cargo Delivery with Neuronal-Cell Compatibility.

We report a conductive semi-interpenetrating polymer network (semi-IPN) hydrogel reinforced with fluorescent carbon quantum dots (CDs) hydrothermally synthesized from branched polyethyleneimine and sodium alginate, together with oxidized carbon nanotubes (ox-CNTs), engineered for sequential dual-cargo delivery with pH-responsive release behavior. The hybrid matrix integrates PEDOT:PSS with a poly(acrylamide-co-2-aminoethylmethacrylate hydrochloride) network to form a robust soft material capable of sequentially releasing a neurotrophic protein and a small-molecule therapeutic. UV-visible decon…

Spatiotemporal delivery of exosomes and alendronate orchestrates osteoblastic and osteoclastic activity for osteoporotic bone regeneration.

The treatment of bone defects in osteoporotic (OP) patients remain a significant clinical challenge. The most important and challenging task … core layer and BMSC-Exo functionalized onto the surface of nanofiber via chemical modification with polyethyleneimine (PEI). Physicochemical characterization confirmed the successful fabrication of PEI@P/PVA-Aln nanofibers, which exhibited excellent mechanical strength (22.77 MPa), hydrophilicity (9.66°), and enabled the spatiotemporal release of BMSC-Exo and Aln. …

Advancing engineered nanoparticles for enhanced efficacy in cancer immunotherapy: optimizing nanoparticles for cancer immunotherapy.

Recent evidence on resistance to immune checkpoint inhibitors (ICIs) has necessitated the exploration of alternative cancer therapies with i … endent efficacy of CAR therapy in all immune cells. Nanoparticles containing cationic polymers like polyethyleneimine (PEI), poly(L-lysine), and poly(2-dimethylamino) ethyl methacrylate effectively delivers DNA to the specific immune cells, thereby increasing the responsiveness towards the CAR therapy. In this review, we highlight promising avenues with potentials …

Multifunctional scaffold integrating chemo/photothermal therapy and bone defect reconstruction for osteosarcoma: An in vitro evaluation.

Osteosarcoma (OS) represents the most common malignant bone tumor in adolescents. The current standard-of-care regimen combines preoperative … l BMP2 delivery systems suffer from initial burst release and dose-dependent complications. Heparin/polyethyleneimine (PEI) nanogels provide an elegant solution through BMP2 encapsulation, enabling sustained release kinetics and reduced systemic toxicity. This study proposes the development of a multifunctional scaffold system for synergistic chemo-photothermal the …

Современный подход к диагностике диабетической ретинопатии

В этой статье рассматриваются инновационные методы диагностики диабетической ретинопатии, поскольку распространение сахарного диабета и его осложнений продолжает расти. Новые методы сосредоточены на раннем обнаружении заболеваний. Технологические новшества, такие как телеофтальмология, смартфонная фотография, искусственный интеллект с глубоким обучением или широкополосная фотография, могут повысить диагностическую точность и ускорить лечение. В ходе обзора в качестве перспективных решений для дистанционного офтальмологического обслуживания рассматриваются телеоптальмология и ручная фотография.

Совпадение в аннотации оригинала
This article reviews innovative diagnostic approaches for diabetic retinopathy as the prevalence of diabetes mellitus and its complications … ety and efficiency. Nanotechnology advancements further enhance diagnosis, offering highly branched polyethyleneimine particles with fluorescein sodium (PEI-NHAc-FS) for better fluorescein angiography or vanadium oxide-based metabolic fingerprinting for early detection.

Visual detection of aldehyde gases using a silver-loaded paper-based colorimetric sensor array.

The small molecule aldehydes are volatile organic compounds (VOCs), possessing cytotoxicity and carcinogenicity. Long-term exposure can pose a serious threat to human health. Based on an in-situ reduction colorimetric method to generate silver nanoparticles and induce colorimetric response, we proposed a silver-loaded paper-based colorimetric sensor array for visually detecting and differentiating five relatively common trace small molecule aldehyde gases. The silver ions are immobilized onto a porous filter paper and stabilized by complexing agents of branched polyethyleneimine, ethylenediami…

Preparation and evaluation of oral insulin nanocapsule delivery systems.

Insulin therapy is essential for regulating blood sugar levels. Conventional subcutaneous injection is prone to psychological stress, local … %, and the loading capacity of insulin was 2.2 %. In addition, cationic modification with chitosan/ polyethyleneimine promoted adhesion and permeation of the intestinal mucus layer, and surface coating with pH-responsive methyl methacrylate trimethylamine ethyl chloride copolymer achieved 100 % gastric protection. The results of rat blood glucose test showed that, …

Smart paper-based materials incorporating nitrogen and boron co-doped MXene quantum dots for rapid adsorption and sensitive detection of Cr2O72.

Dichromate ion (Cr2O72-) is a highly toxic chromium-containing compound that poses significant hazards to the digestive, respiratory systems, skin, and mucous membranes. Currently, the detection and adsorption of Cr2O72- face significant challenges, including the time-consuming and low sensitivity nature of traditional analytical methods. The limited efficiency and capacity of existing adsorbents hinder their practical application in real-time water quality monitoring and environmental remediation. Herein, using polyethyleneimine-functionalized (PEI) pulp fiber paper as the substrate, we devel…