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m6A mRNA methylation initiated by METTL3 directly promotes YAP translation and increases YAP activity by regulating the MALAT1-miR-1914-3p-YAP axis to induce NSCLC drug resistance and metastasis.

BackgroundMETTL3 is an RNA methyltransferase that mediates m6A modification and is implicated in mRNA biogenesis, decay, and translation. However, the biomechanism through which METTL3 regulates MALAT1-miR-1914-3p-YAP axis activity to induce NSCLC drug resistance and metastasis is not very clear.MethodsThe expression of mRNA was analyzed by qPCR assays. Protein levels were analyzed by western blotting and immunofluorescent staining. Cellular proliferation was detected by CCK8 assays. Cell migration and invasion were analyzed by wound healing and transwell assays, respectively. Promoter activit

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… Furthermore, we performed RT-PCR, WB, and qPCR assays to confirm that co-treatment with METTL3 and YTHDF3 or siYTHDF3 regulates the expression of E-cadherin and vimentin. … Vimentin protein levels were further increased upon the co-transfection of METTL3 and YTHDF3 compared to those detected upon co-transfection with METTL3 and siYTHDF3 . …

Olive phenolics as c-Met inhibitors: (-)-Oleocanthal attenuates cell proliferation, invasiveness, and tumor growth in breast cancer models.

Dysregulation of the Hepatocyte growth factor (HGF)/c-Met signaling axis upregulates diverse tumor cell functions, including cell proliferation, survival, scattering and motility, epithelial-to-mesenchymal transition (EMT), angiogenesis, invasion, and metastasis. (-)-Oleocanthal is a naturally occurring secoiridoid from extra-virgin olive oil, which showed antiproliferative and antimigratory activity against different cancer cell lines. The aim of this study was to characterize the intracellular mechanisms involved in mediating the anticancer effects of (-)-oleocanthal treatment and the potent

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… However, vimentin was below the level of detection in MCF-7 and BT-474 cells. In addition, (-)-oleocanthal treatment showed cell line-specific change of β-catenin levels. … Vimentin is an intermediate filament protein normally expressed in cells of mesenchymal origin [40] . Vimentin regulates cell migration in many cell types [40] . …

Exosomal AFAP1-AS1 binds to microRNA-15a-5p to promote the proliferation, migration, and invasion of ectopic endometrial stromal cells in endometriosis.

BackgroundEndometriosis (EMS) remains a major challenge to reproductive health due to multifactorial etiology, disease heterogeneity, and the lack of appropriate diagnostic markers and treatment. Eexosome (Exo) has become a major factor in progression of a variety of diseases. However, the mechanisms directing their role in the pathophysiology of EMS are ill-defined. Here, we aimed to investigate the clinical implications of actin filament associated protein 1-Antisense RNA 1 (AFAP1-AS1) in EMS.MethodsBioinformatics analysis was used to predict the expression and interaction of AFAP1-AS1, miR-

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… After purification, the morphology was observed by immunofluorescence staining and the expression of vimentin in the cells was identified by immunofluorescence and immunohistochemistry (IHC). … A, Immunofluorescence to observe the morphology of each group of cells and the expression of Vimentin protein (scale bar = 200 μm). B, IHC to detect Vimentin protein expression in each group of cells (scale bar = 50 μm). Additional file 2: Supplementary Table 1. Primer sequences for RT-qPCR. …

Вирус Эпштейн-Барр и иммунная микроокружающая среда в множестве склерозов: Отличения от высокомерной съемки тканей мозга.

Вирус Эпштейн-Барр (ЭБВ) во многом связан с патогенезом множественного склероза (МС), однако его точная роль в прогрессии заболеваний остается неясной. С использованием высокомерной системы обнаружения СО путем индексации, технологии пространственной съемки, в настоящем исследовании изучается клеточная микроокружающая среда поражения MS в вторичных прогрессивных MS и первичных прогрессивных MS. Мы проанализировали взаимодействие иммунной, глиальной, нейрональной и эндотелиальной клеток в MS-повреждениях и обычно встречающейся белой материи в двух независимых группах. Наши результаты свидетельс

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… An interaction between Vimentin+ smooth muscle cells and CD45RO+ memory T cells was also noted (log odds = 0.38, P = 0.049) ( SI Appendix , Fig. S4 C ). Immune cell enrichment and interactions in SPMS vs. … Increased interactions between AQP4+ astrocytes, synaptophysin, and Claudin-5, alongside the prevalence of Vimentin+ and GlialCaM+ astrocytes, highlight the disruption of glial–neuronal interactions. …

SQSTM1/p62 promotes the progression of gastric cancer through epithelial-mesenchymal transition.

BackgroundSQSTM1/p62 is an autophagy-related receptor protein that participates in regulating tumorigenesis and multiple signaling pathways. … chymal transition (EMT), leading to upregulation of E-cadherin and downregulation of N-cadherin and vimentin.ConclusionThis study provides novel and robust evidence for the mechanism by which elevated p62 expression promotes the progression of gastric cancer. It offers promising therapeutic targets for anti-tumor treatment strategies in gastric cancer pati …

Exploring The Role of TOP2A in the Intersection of Pathogenic Mechanisms Between Rheumatoid Arthritis and Idiopathic Pulmonary Fibrosis Based on Bioinformatics.

BackgroundRheumatoid arthritis (RA) and idiopathic pulmonary fibrosis (IPF) share a common pathogenic mechanism, but the underlying mechanisms remain ambiguous. Our study aims at exploring the genetic-level pathogenic mechanism of these two diseases.MethodsWe carried out bioinformatics analysis on the GSE55235 and GSE213001 datasets. Machine learning was employed to identify candidate genes, which were further verified using the GSE92592 and GSE89408 datasets, as well as quantitative real-time PCR (qRT-PCR). The expression levels of TOP2A in RA and IPF in vitro models were confirmed using West

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… Our qRT-PCR results indicated that PluriSIn#2 could significantly suppress the expression of col1a1, col3a1, fn1, vimentin, il-6 , and cox-2 mRNA in SW982 induced by TNF-α ( Figure 7A ). … We also found that PluriSIn #2 could also inhibit the protein expression of COL I, COL III, VIMENTIN, FN1, IL-6 and COX-2 in RA in vitro by Western-Blotting ( Figure 7B ). …

Osteoclasts in Tumor Biology: Metastasis and Epithelial-Mesenchymal-Myeloid Transition.

Osteoclast is a specialized cell that originates from monocytic lineage, communicates closely with osteoblasts under physiological conditions, participates in bone modeling and re-modeling, contributes to calcium homeostasis and osteoimmunity. In pathological conditions, it is involved in many tumors such as giant cell bone tumor (osteoclastoma), aneurysmal bone cyst, osteosarcoma, and metastatic cancers, and it usually causes local spread and progression of the tumor, working against the host. Since osteoclasts play an active role in primary bone tumors and bone metastases, the use of anti-os

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… These molecules include Snail, ZEB, Twist1, vimentin, N-cadherin, and fibronectin, which tumor cells use to pass intravascularly from the primary area and for their intravascular survival. … Mononuclear cells show positive staining with mesenchymal and myeloid markers such as CD68, vimentin, LCA, SMA, and S-100, in varying proportions. …

Резюме

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… The diagnosis is based on S-100 and vimentin positivity in the absence of other lineage markers. Follicular dendritic cell sarcoma (FDCS) occurs in adults and more often presents in the lymph node, at times within the context of Castleman disease. In 90% of cases, it has an indolent behaviour. …

MicroRNA-150-5p inhibits the proliferation and invasion of human larynx epidermiod cancer cells though regulating peptidyl-prolyl cis/trans isomerase.

ObjectiveThis study aimed to investigate the molecular mechanism of miR-150-5p regulating the malignant biological behavior of Human Epidermoid cancer cell (HEp-2) by targeting peptidyl-prolyl cis/trans isomerase NIMA-Interacting-1 (PIN1).MethodsFirstly, qRT-PCR and Western blot were adopted to detect the expression levels of miR-150-5p and PIN1 in cancer tissue and paracancerous tissues of patients with LSCC, and those in human bronchial epithelial cells (16 HBE) and HEp-2. Next, the targeted relationship between miR-150-5p and PIN1 was assessed by bioinformatics website and dual-luciferase r

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… Shortly speaking, in the miR-150-5p mimics group, protein expression levels of MMP-2, MMP-9, Vimentin and N-cadherin in cells were notably down-regulated ( p < 0.01) while the E- cadherin expression was markedly raised ( p < 0.01). … Nevertheless, miR-150-5p inhibitor group displayed a significant increase in the expression levels of MMP-2, MMP-9, Vimentin and N-cadherin ( p < 0.01) while a notable reduction in the E-cadherin expression ( p < 0.01) ( Fig. 3 A‒B). …

Роль Neurofilament fight (NfL) и глиального фибрового кислотного белка (GFAP) в MS и AQP4-NMOSD: развитие клинического применения.

Важную роль в диагностике, мониторинге и оценке терапевтической реакции в таких условиях, как мультипликативный склероз (MS) и аквапорин-4 Нейромиелит Оптика Спектрум (AQP4-NMOSD), играют плавучие биомаркеры, такие, как глиальные фибриллярные протеины (GFAP) и Neurofilament Light (NfL). Эти биомаркеры позволяют получить ключевые сведения о патофизиологических механизмах, лежащих в основе этих заболеваний, что позволяет осуществлять эффективные последующие мероприятия и применять индивидуализированные методы лечения, которые имеют существенно важное значение для улучшения результатов лечения па

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… As a type-III intermediate filament, it is part of a class that also includes vimentin, desmin, and peripherin, all essential to the structure and function of the cell's cytoskeleton. GFAP, encoded by gene on chromosome 17q21, consists of 432 amino acids. … At the quaternary level, GFAP can form both homopolymeric filaments from GFAP alone and heteropolymeric filaments with other type III intermediate filaments like vimentin. …

Therapeutic Apheresis Using a β2-Microglobulin Removal Column Reduces Circulating Tumor Cell Count.

An elevated serum β2-microglobulin (β2M) level is indicative of impaired glomerular filtration and prerenal diseases, such as malignant tumors, autoimmune disorders, and liver diseases. An elevated serum β2M level has been shown to promote metastasis via the induction of epithelial-mesenchymal transition (EMT) in cancer cells. However, the therapeutic potential of targeting β2M remains unclear. Here, we aimed to investigate the efficacy of Filtor, a small polymethyl methacrylate fiber-based β2M removal column, in reducing the β2M level and suppressing cancer cell-induced EMT and metastasis. We

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… Nihon Gene Research Laboratories Inc. labelled CTCs that were positive for vimentin and negative for cytokeratin (CK) expression as “Type 1s” if they were single-celled or “Type 1c” if they were clustered, whereas single-celled and clustered CTCs negative for vimentin and positive CK expression were … The removal of vimentin-positive cells via Filtor in this study may be related to the reported correlation between PDPN and vimentin levels in various cancer types [ 20 , 21 , 22 , 23 ]. …

Pluripotent Stem Cells for Disease Modeling and Drug Discovery in Niemann-Pick Type C1.

The lysosomal storage disorders Niemann-Pick disease Type C1 (NPC1) and Type C2 (NPC2) are rare diseases caused by mutations in the NPC1 or NPC2 gene. Both NPC1 and NPC2 are proteins responsible for the exit of cholesterol from late endosomes and lysosomes (LE/LY). Consequently, mutations in one of the two proteins lead to the accumulation of unesterified cholesterol and glycosphingolipids in LE/LY, displaying a disease hallmark. A total of 95% of cases are due to a deficiency of NPC1 and only 5% are caused by NPC2 deficiency. Clinical manifestations include neurological symptoms and systemic

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… In addition, GFAP and vimentin had been shown to be hypophosphorylated, subsequently leading to a polymerization of intermediate filament monomers, resulting in a criss-cross-like appearance of vimentin and GFAP in the cytosol. … Regarding vimentin, comparable results were described for NPC1 patient-derived fibroblasts [ 89 , 90 ]. The authors described alterations of vimentin based on its hypophosphorylation due to a decreased activity of protein kinases. …

Imaging and surgical outcomes of spinal tumors in 18 dogs and one cat.

Clinical and magnetic resonance imaging (MRI) findings, histological appearances and surgical outcomes of 18 dogs and one cat with spinal tumors are presented. Medical records of the cases admitted for spinal disorders were reviewed, and cases of spinal tumors that were diagnosed by MRI and confirmed by histological examination were included in this study. T1 weighted, T2 weighted and contrast enhanced T1 weighted images were taken and interpreted to evaluate the spinal tumors. The tumors were diagnosed as: meningioma (n = 6), ependymoma (n = 1), nerve sheath tumor (n = 4), metastatic spinal t

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… Upon histological examination, the ependymoma was composed of pseudorosettes, and immunohistochemical examination revealed vimentin and glial fibrillary acidic protein immunoreactivity in the neoplastic cells (panel C in Fig. 4 ). … Conversely, fibrovascular stroma and capsules were stained α-SMA and vimentin sera. Discussion Characteristic signs and location of spinal tumor in MRI provided ante-mortem prediction of the various histological types of neoplasms. …

Protective effects of engineered Lactobacillus crispatus strains expressing G-CSF on thin endometrium of mice.

STUDY QUESTION: Does recombinant Lactobacillus expressing granulocyte colony-stimulating factor (G-CSF) have a better protective effect than … vessels and glands (both P < 0.001), enhanced the expression of cytokeratin 19 (CK19) (P < 0.001), vimentin (Vim) (P < 0.001), vascular endothelial growth factor-A (P < 0.001), and CD34 (P < 0.001), and decreased fibrosis levels (P = 0.004). In addition, the high-throughput sequencing results indicated that the L. crispatus-pPG612-G-CSF strain could decre …

Inhibition of cytochrome P450 epoxygenase promotes endothelium-to-mesenchymal transition and exacerbates doxorubicin-induced cardiovascular toxicity.

BackgroundDoxorubicin (DOX) is a potent chemotherapy widely used in treating various neoplastic diseases. However, the clinical use of DOX is limited due to its potential toxic effect on the cardiovascular system. Thus, identifying the pathway involved in this toxicity may help minimize chemotherapy risk and improve cancer patients' quality of life. Recent studies suggest that Endothelial-to-Mesenchymal transition (EndMT) and endothelial toxicity contribute to the pathogenesis of DOX-induced cardiovascular toxicity. However, the molecular mechanism is yet unknown. Given that arachidonic acid a

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… Unexpectedly, while DOX did not significantly affect Platelet endothelial cell adhesion molecule (i.e., CD31) and significantly reduced the protein expression of vimentin compared to control (Table 1 ), cells treated with a combination of DOX and MSPPOH demonstrated a decrease in the level of vimentin … I snail-1 (sna-1), J collagen1a1 (col1a1), K vimentin (vit) and L transforming growth factor-β (tgf-β). Results are shown as means ± SEM. …

Обязательный экзамен по патологии: лишний или необходимый?

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B-cells in pulmonary arterial hypertension: friend, foe or bystander?

There is an unmet need for new therapeutic strategies that target alternative pathways to improve the prognosis of patients with pulmonary arterial hypertension (PAH). As immunity has been involved in the development and progression of vascular lesions in PAH, we review the potential contribution of B-cells in its pathogenesis and evaluate the relevance of B-cell-targeted therapies. Circulating B-cell homeostasis is altered in PAH patients, with total B-cell lymphopenia, abnormal subset distribution (expansion of naïve and antibody-secreting cells, reduction of memory B-cells) and chronic acti

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… If HUVECs are used, anti-endothelial cell antibodies from both patient groups recognise cytoskeletal (lamin A/C, tubulin-β chain, vimentin) and ribosomal proteins [ 79 ]. … Additionally, plasma from MCT rats labels pulmonary artery adventitia, suggesting reactivity against fibroblast antigens at the pulmonary level; and vimentin, PI3K and HSP27 are confirmed as targets of antifibroblast antibodies in this model [ 60 ]. …

Emerging evidence of the molecular landscape specific for hematogenous metastasis from gastric cancer.

Gastric cancer (GC) is one of the most frequently diagnosed cancers in the world. Most GC patients are diagnosed when the cancer is in an advanced stage, and consequently, some develop metastatic lesions that generally cause cancer-related death. Metastasis establishment is affected by various conditions, such as tumor location, hemodynamics and organotropism. While digestive cancers may share a primary site, certain cases develop hematogenous metastasis with the absence of peritoneal metastasis, and vice versa. Numerous studies have revealed the clinicopathological risk factors for hematogeno

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… Vimentin Vimentin (VIM) is a type III intermediate filament protein that is mainly expressed in mesenchymal cells and an important marker of epithelial mesenchymal transition (EMT)[ 13 ]. … The 3-year survival of the patients with vimentin-positive GC was significantly poorer than that of patients with vimentin-negative GC. VIM positivity was an independent prognostic factor in multivariate analysis with respect to overall survival. …

Mobilizing stem cells and inhibition of autoimmunity synergistically modify autoimmune disease to relieve pain: a potential MRG-001 therapy for rheumatoid arthritis.

OBJECTIVES: Rheumatoid arthritis (RA) is a chronic autoimmune disease characterized by persistent synovial inflammation, bone erosion, cartilage destruction, and debilitating pain. Current therapies often fall short in preventing structural damage and restoring immune balance. To address these limitations, we evaluated MRG-001 - a fixed-dose combination of plerixafor (AMD3100) and low-dose tacrolimus (FK506) with reported immunoregulatory and regenerative potential - in preclinical models of RA. METHODS: The therapeutic efficacy of MRG-001 was assessed in collagen-induced arthritis and collag

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… FLSs express canonical fibroblast markers such as vimentin [ 48 , 49 ] ; therefore, we assessed the presence of vimentin + FLSs in the joints of CIA mice. Fewer vimentin + FLSs were detected in the joints of non-immunized DBA-Veh mice ( Fig. 3A – C ). … By 6 weeks post-treatment, the number of vimentin + FLSs remained elevated in saline- and single-agent treated CIA mice, while MRG-001-treated mice exhibited fewer vimentin + FLSs compared to other groups in both subchondral bone and synovium ( Fig. 3D – F ). …

An overview of the potential anticancer properties of cardamonin.

Cancer is one of the leading causes of mortality, contributing to 9.6 million deaths globally in 2018 alone. Although several cancer treatments exist, they are often associated with severe side effects and high toxicities, leaving room for significant advancements to be made in the field. In recent years, several phytochemicals from plants and natural bioresources have been extracted and tested against various human malignancies using both in vitro and in vivo preclinical model systems. Cardamonin, a chalcone extracted from the Alpinia species, is an example of a natural therapeutic agent that

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… Cardamonin also caused the downregulation of Snail, Slug, and Vimentin, crucial mesenchymal markers [ 37 – 39 ]. Through these mechanisms, cardamonin has been proven to have inhibitory effects against breast cancer cell lines. … Cardamonin also suppressed the expression of the EMT markers including Snail, Slug, and Vimentin, and increased E-cadherin expression levels. Another study emphasized the useful effect of cardamonin when used in combination with 5-FU. …