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[Application of a simple method for detection of rubella virus genome by loop-mediated isothermal amplification (LAMP)].

OBJECTIVE: To use a new simple Reverse Transcription Loop-mediated isothermal amplification (RT-LAMP) method was applied to detect rubella virus nucleic acid and compared with Reverse Transcription-Polymerase Chain Reaction (RT-PCR). METHOD: Comparing the detection rate of the RT-LAMP method with that of RT-PCR for detecting rubella virus nucleic acid from rubella virus. RESULTS: The nucleic acid positive rates of all 11 strains of rubella virus were 100% by the two methods, the positive rate was 55%. CONCLUSION: RT-LAMP is more simple and convenient than RT-PCR.

Predominance of CTX-M-type extended-spectrum beta-lactamase genes among enterobacterial isolates from outpatients in Brazil.

Two hundred fifty-seven nalidixic acid-resistant enterobacterial isolates were collected in a Brazilian community from January 2000 to May 2005 to determine the prevalence of plasmid-encoded extended-spectrum beta-lactamases. The bla(CTX-M) genetic environment was determined by polymerase chain reaction and sequencing. Eleven isolates (4.2%) harbored a bla(CTX-M-2) gene, 3 isolates bla(CTX-M-9), 2 isolates bla(CTX-M-8), and 6 isolates bla(SHV-5). Two novel bla(CTX-M-2) variants, namely, bla(CTX-M-74) and bla(CTX-M-75), were identified.

Herpes zoster induced acute urinary retention in the immunocompetent female.

We present a rare case of acute urinary retention complicated by constipation secondary to a unilateral herpes zoster reactivation in the S2-4 dermatomes of an immunocompetent female. Diagnosis was confirmed by clinical examination, negative cystoscopy and positive viral polymerase chain reaction (PCR) for herpes zoster virus. The patient was commenced on a course of oral acyclovir, the bowel symptoms resolved, and the patient was discharged with a urinary catheter in situ for an outpatient trial without catheter for 2 weeks to be followed by a course of intermittent self catheterisation pendi…

Going viral: fusiform vertebrobasilar and internal carotid aneurysms with varicella angiitis and common variable immunodeficiency.

Intracranial aneurysms in the pediatric population are relatively rare entities. Immunocompromised patients (often from HIV/AIDS or pharmacological immunosuppression) represent a significant fraction of children with cerebral aneurysms. One proposed mechanism of aneurysm formation in these patients is from direct infection of the affected arteries. In this study, the authors report on a case of a 14-year-old girl with common variable immunodeficiency with T-cell dysfunction and a CSF polymerase chain reaction test positive for varicella-zoster virus who underwent evaluation for carotid and bas…

[Eczema herpeticum caused by herpes simplex virus type 2. Review of the literature about one case].

Eczema herpeticum is an extensive vesicular eruption caused by cutaneous dissemination of herpes simplex virus in patients who have underlying skin conditions or epidermal barrier disruption. Herpes simplex virus type 1 (HSV 1) is the most common etiology. This is the first report in Chile of a case of eczema herpeticum caused by HSV 2 in a patient with Darier's disease, We review the literature and emphasize the use of sensitive and specific assays as real time polymerase chain reaction, that allow to initiate timely the correct treatment.

Methotrexate-associated mantle-cell lymphoma in an elderly man with myasthenia gravis.

BACKGROUND: A 75-year-old man on methotrexate immunosuppression for myasthenia gravis presented with a 2-month history of lymphocytosis and bilateral inguinal adenopathy. There were no constitutional symptoms of fever, night sweats, or weight loss. INVESTIGATIONS: Physical examination, blood tests, flow cytometry, fluorescent in situ hybridization, immunoglobulin gene sequencing, viral load quantification by real-time polymerase chain reaction, excisional lymph-node biopsy, bone-marrow biopsy, tumor morphology and immunohistochemistry, sequential CT and PET scans. DIAGNOSIS: Methotrexate-ass…

[Helicobacter pylori in patients with bleeding ulcer].

The diagnostic sensitivity of the polymerase chain reaction (PCR) and urease respiratory test (URT) in H. pylori infection are compared. The efficacy of H. pylori eradication in patients with a bleeding peptic ulcer using three-component scheme (De-nol, Clarythromycin, Furazolidon) and a complex medication Pylobact is also compared. Thus, URT, performed within 24 hours after the admission is more effective in patients with bleeding ulcer, then PCR. H. pylori eradication is more successful with Pylobact (93,8%) and has less side-effects in such patients, then the used three-component eradicatio…

[Chancre of the finger. A circuitous route to diagnosis].

A 33-year-old woman presented with a 4-months history of a granulating ulcer on the right index finger. Paronychia was suspected and nail extraction with subsequent histopathologic examination of the removed tissue was performed. Two months later, it became known that the patient's sexual partner had been treated for syphilis. The patient's serology was also positive. Subsequent examination of the original tissue sample by polymerase chain reaction, immunohistochemistry using Treponema pallidum-specific antibodies, and silver staining revealed large numbers of syphilis bacteria, confirming the…

[Detection of infectious agents in heart valves during endocarditis using PCR technique].

Infectious endocarditis can be caused by various microorganisms. Diagnostics of local infection by microbiological methods is not always effective. For that reason we performed a study aimed for direct detection of potential infectious agents by polymerase chain reaction in patients' heart valve tissue. DNA of infectious agents was revealed in 72% of heart valve tissue samples from patients with septic endocarditis; in studied samples, along with bacterial DNA, herpesviruses' DNA was detected. Obtained results confirm the presence of infection, which allows to perform specific diagnostics of i…

Cytomegalovirus papillitis in an immunocompetent patient.

A 22-year-old immunocompetent woman who presented with a 3-week history of fever, headache, and visual loss and was found to have subnormal visual acuity and bilateral optic disc swelling. Serum cytomegalovirus (CMV) IgM and IgG and polymerase chain reaction results were positive, indicating an acute CMV infection. No cause of immunocompromise was found. After treatment with intravenous ganciclovir, the papillitis and systemic CMV illness resolved with no residual deficit. This is the first reported case of primary CMV papillitis to be successfully treated with ganciclovir alone.

Detection of Japanese encephalitis virus antibodies in bats in Southern China.

In this study, 336 bats of eight species were collected during 2004-2007 in six provinces of Southern China. Antibodies against Japanese encephalitis virus (JEV) were detected by ELISA in 43 of 336 (12.8%) serum samples. Among those ELISA-positive samples, 11 serum samples had neutralizing antibodies against JEV. No JEV was detected in brain and liver samples using specific real-time reverse transcriptase-polymerase chain reaction. This is the first report of JEV neutralizing antibodies in bats in China, which reinforces that bats can be involved in the life cycle of this virus.

Prevalence of ETV6-RUNX1 fusion gene in children with acute lymphoblastic leukemia in China.

A series of 92 Chinese children newly diagnosed with acute lymphoblastic leukemia (ALL) were examined for the ETV6-RUNX1 (previously TEL-AML1) fusion gene by using a nested reverse transcriptase-polymerase chain reaction. ETV6-RUNX1 fusion transcripts were detected in 21 of 92 patients (22.8%): 16 with common ALL, 4 with precursor B-cell ALL, and 1 with T-ALL. The prevalence of ETV6-RUNX1 positivity was 24.7% (20/81) in childhood B-lineage ALL. The prevalence of ETV6-RUNX1 in Chinese pediatric ALL patients proved to be similar to that found in other countries.

The first outbreak of camelpox in Syria.

In this study we report the first outbreak of camelpox in two provinces in Syria. Clinical symptoms started with fever, salivation and general exanthema. The main features were facial and legs oedema, pustules on the mucosa of the lips and a high rate of abortion. Lesions may also occur on the whole body including scrotum and udder. Specimens were investigated by electron microscopy, virus isolation in cell culture and embryonated eggs and by immunohistochemistry. The causative agent was identified as camelpox virus by polymerase chain reaction and sequencing of the hemagglutinin gene.

Leishmania major infection in a patient with visceral leishmaniasis: treatment with Amphotericin B.

We have reported an acute form of visceral leishmaniasis, which was caused by Leishmania major, in Southern Iran. The parasite was isolated from a 30-year-old man living in Bushehr Province, Southern Iran, and an endemic region of kala-azar in south of Iran. The patient had typical clinical signs of Mediterranean kala-azar, and besides parasitological, biochemical, and immunological findings confirming diagnosis of visceral leishmaniasis, the agent was identified as L. major using Nested polymerase chain reaction. The patient was treated successfully with Amphotericin B during a 1-month period…

First evidence of Babesia gibsoni (Asian genotype) in dogs in Western Europe.

Canine babesiosis in Europe is generally caused by Babesia canis canis and Babesia canis rossi. Here we describe the first two autochthonous cases of Babesia gibsoni (Asian genotype) infection in Germany. Two American pit bull terriers showed clinical and hematologic signs consistent with babesiosis. Polymerase chain reaction (PCR) analysis of the 18S rDNA of blood samples revealed 486 bp fragments. The sequences were 100% identical to each other and 100% identical to Babesia gibsoni (Asian genotype). These results represent the first genetic evidence of Babesia gibsoni (Asian genotype) parasi…

Atovaquone and azithromycin treatment for babesiosis in an infant.

An 8-month-old infant with cyanotic heart disease and transfusion-associated Babesia microti infection is reported here. At initial presentation, she was ill appearing, febrile and cyanotic. Laboratory tests revealed severe anemia, thrombocytopenia and an increase in hepatic enzymes. The diagnosis was made by the presence of intraerythrocytic parasites on thin blood smear and confirmed by serology and polymerase chain reaction. The infant was treated successfully with a combination of oral azithromycin and atovaquone. This combination is an alternative to clindamycin and quinine for the treatm…

Dengue haemorrhagic fever complicated by eclampsia in pregnancy.

A 28-year-old primigravida presented at 36 weeks of gestation with a one-week history of fever with myalgia. Diagnosis of dengue fever was made based on viral polymerase chain reaction. She progressed to dengue shock syndrome by day nine and subsequently recovered. She delivered a healthy male baby by the vaginal route, but within 24 hours of delivery, had an eclamptic seizure, which was controlled with intravenous magnesium sulphate. Mother and the baby were well at discharge and on the follow-up visit at three months.

Visceral leishmaniosis and parapoxvirus infection in a Mediterranean monk seal (Monachus monachus).

A Mediterranean monk seal was shown by immunohistochemical and polymerase chain reaction techniques to be dually infected with a Leishmania sp. and parapoxvirus. The pathological findings included a deep ulcer on the side of the head, ulcers on the gingival and inner aspect of the lower lip, enlarged lymph nodes and tonsils, and respiratory lesions (pulmonary consolidation, oedema, haemorrhages and emphysema; tracheal and bronchial congestion, exudates and haemorrhage). Amastigotes were demonstrated in macrophages in the lymph nodes and spleen, and intracytoplasmic inclusion bodies were observ…

Enterovirus detection as a result of West Nile virus surveillance.

In this 2005 study from the Los Angeles County Public Health Department Laboratory, Los Angeles, CA, cerebrospinal fluid specimens submitted for West Nile virus (WNV) serologic testing were also tested for enterovirus by real time reverse transcription-polymerase chain reaction and culture. Approximately 10% of the WNV- specimens were positive for enterovirus. Altered consciousness and muscle weakness were more common with WNV infection. Results provide compelling evidence that laboratory testing is a necessary adjunct to clinical diagnosis for accurate discrimination among the various possibl…

Доклад о деле: Борелия валесиана инфекция японского человека, связанного с путешествием в зарубежные страны.

78-летний японец, у которого были клинические симптомы и сходная с гриппом болезнь лихорадкой, охлаждением, диареей и архальгией, до появления симптомов и болезней поехал в Камбоджу и Хабаровск, Россия. Он был укушен клещами Ixodes persulcatus, в которых была обнаружена ДНК Боррелии валезианы. Симптомы пациента быстро улучшались после лечения с помощью микроциклина. Серологический осмотр выявил антитела от болезни Лайма Борелия. Цепная реакция FlaB полимеразы с плазмой пациента усилила фрагмент ДНК, похожий на фрагмент B. valaisiana.

Совпадение в аннотации оригинала
A 78-year-old Japanese man who had clinical symptoms and a flu-like illness with fever, chills, diarrhea, and arthralgia had traveled to Cam … tment with minocycline. Serologic examination detected antibodies to Lyme disease Borrelia. An flaB polymerase chain reaction with the patient's plasma amplified a DNA fragment similar to that of B. valaisiana.