Результаты поиска

Integrated metabolomics, transcriptomic, and phytohormonal analyses to study the effects of water stress and foliar abscisic acid application in Thymus species using LC-MS/MS.

Thyme species, including Thymus vulgaris, T. kotschyanus (drought-tolerant) and T. serpyllum (drought-sensitive), are valuable medicinal herbs. They are often grown in arid regions and are increasingly suffering from water stress due to climate change. Here, we analyzed the metabolome and expression of selected genes in leaves of these species under drought stress with and without treatment with the phytohormone abscisic acid (ABA). Among the terpenes, dominant metabolites in thyme, thymol was the most important terpenoid component, followed by thymoquinone, carvacrol and p-cymene in all three

Mechanism of Red Yeast Rice in the Improvement of Atherosclerosis in Apolipoprotein E-deficient Mice Explored Through Metabolomics Combined with Serum Pharmacochemistry and Network Pharmacology.

Red Yeast Rice (RYR), the fermentation of cooked rice kernels with Monascus purpureus, has been traditionally and increasingly applied to alleviate atherosclerosis (AS). Nonetheless, the precise mechanisms underlying its therapeutic effects in AS treatment remain elusive. Metabolomics combined with serum pharmacochemistry and network pharmacology was utilized to explore the potential molecular mechanisms by which RYR improves AS. RYR showed a significant inhibitory effect on metabolic dysfunction in mice. Specifically, through metabolomics analysis, 45 metabolic biomarkers were identified, whi…

Plasma Lyso-PE 22:6 and Lyso-PE 20:4 are associated with development of mild to moderate depression revealed by metabolomics: a pilot study.

BACKGROUND: Mild to moderate depression (MMD), as an early stage of depression, has a high incidence and may progress to severe depression, even leading to suicide. The lack of effective screening and treatment is due to the unknown metabolic changes and processes in patients with MMD. Here, we performed metabolite profiling to investigate the key metabolites associated with MMD in plasma. METHODS: A high-throughput metabolomics method using ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS) was used to determine the metabolic…

UPP1 as a potential target for astilbin in ameliorating high-fat diet-induced bone loss via MAPK signaling: a study incorporating gut microbiota and metabolomics.

This study aims to investigate the therapeutic effects and underlying mechanisms of astilbin on high-fat diet (HFD)-induced bone loss, focus … icro-CT), and bone metabolism markers were assessed. Transcriptomic, 16S rRNA sequencing, and serum metabolomics analyses were performed to explore the molecular mechanisms. In vitro experiments using bone marrow-derived mesenchymal stem cells (BMSCs) were conducted to evaluate osteogenic and adipogenic differentiation under palmitic acid and astilbin treatmen …

[Equivalence and mechanism of standard decoction and formula granules of calcined oyster in treatment of rats with ethanol-induced gastric ulcers based on serum metabolomics].

An ethanol-induced gastric ulcer model was replicated to investigate the equivalence and metabolic regulatory mechanisms of formula granules and standard decoction of calcined oyster by using metabolomics. The ethanol-induced gastric ulcer model was established, and the dose-effect curve was fitted with the ulcer inhibition rate as the effect to compare the equal-effect dose(DEE) and equal-dose effect(EED) of standard decoction and formula granules of calcined oyster. The formula granule group and standard decoction group with the equivalent effect were selected for further pharmacodynamic ind…

Разработка и утверждение нового метода ЛК-МС/МС для анализа плазматических периферальных кислот коротко- и среднекалиберного толстого происхождения для применения метаболомики.

Справочная информация: Короткоцепные жирные кислоты (СКФА) и среднецепные жирные кислоты (МСФА) представляют собой метаболиты человека, которые участвуют в различных биохимических процессах и могут предложить ценные знания и информацию по различным патологическим и метаболическим проблемам пациентов. Точные, точные, высокоэффективные биоаналитические методы являются важными инструментами как в исследованиях, так и в диагностике многих патологий, при этом ЛК-МС являются наиболее часто используемой методологией в современных метаболомических исследованиях. Методы: В настоящем документе описывает

Совпадение в аннотации оригинала
Background: Short-chain fatty acids (SCFAs) and medium-chain fatty acids (MCFAs) are human metabolites which are involved in various biochem … nd diagnostics of many pathologies, with LC-MS being the most frequently used methodology in modern metabolomics studies. Methods: The current paper describes a complete LC-MS/MS methodology for the accurate quantification of total plasmatic SCFA concentrations in humans using high-resolution QTOF mass spectrometric detection, including sample cleanup, prepara …

Distinct Changes in Metabolic Profile and Sensory Quality with Different Varieties of Chrysanthemum (Juhua) Tea Measured by LC-MS-Based Untargeted Metabolomics and Electronic Tongue.

Chrysanthemum tea, a typical health tea with the same origin as medicine and food, is famous for its unique health benefits and flavor. The taste and sensory quality of chrysanthemum (Juhua) tea are mainly determined by secondary metabolites. Therefore, the present research adopted untargeted metabolomics combined with an electronic tongue system to analyze the correlation between the metabolite profiles and taste characteristics of different varieties of chrysanthemum tea. The results of sensory evaluation showed that there were significant differences in the sensory qualities of five differe…

The active components and potential mechanisms of Wuji Wan in the treatment of ethanol-induced gastric ulcer: An integrated metabolomics, network pharmacology and experimental validation.

ETHNOPHARMACOLOGICAL RELEVANCE: Wuji Wan (WJW) is a traditional Chinese medicine formula that can be found in the "Prescriptions of Taiping … e components that were absorbed into the blood of WJW and its metabolites. Network pharmacology and metabolomics were subsequently used to identify the targets and pathways. Real-time quantitative PCR (RT‒qPCR) and Western blotting were used to verify the mRNA and protein levels of the key targets and pathways. Finally, the active components were identified by …

Exploring antithrombotic mechanisms and effective constituents of Lagopsis supina using an integrated strategy based on network pharmacology, molecular docking, metabolomics, and experimental verification in rats.

ETHNOPHARMACOLOGICAL RELEVANCE: Thrombosis is a common cause of morbidity and mortality worldwide. Lagopsis supina (Stephan ex Willd.) Ikonn … nd mechanism were evaluated in an arteriovenous bypass thrombosis rat model. In addition, the serum metabolomics analysis was conducted using ultra-high performance liquid chromatography coupled with Q-Exactive mass spectrometry. RESULTS: A total of 124 intersected targets of L. supina against thrombosis were predicted. Among them, 24 hub targets were obtaine …

A combination of physiology, metabolomics, and genetics reveals the two-component system ResS/ResR-mediated Fe and Al release from biotite by Pseudomonas pergaminensis F77.

Understanding of the mechanisms on bacteria-regulated mineral dissolution functions is important for further insight into mineral-microbe interactions. The functions of the two-component system have been studied. However, the molecular mechanisms involved in bacterial two-component system-mediated mineral dissolution are poorly understood. Here, the two-component regulatory system ResS/ResR in the mineral-solubilizing bacterium Pseudomonas pergaminensis F77 was characterized for its involvement in biotite dissolution. Strain F77 and the F77ΔresS, F77ΔresR, and F77ΔresS/R mutants were construct

16S rRNA Gene Sequencing Combined with Metabolomics to Explore Intestinal Flora and Metabolic Changes in Young Febrile Rats and the Mechanism of Xiangqin Jiere Granules.

PURPOSE: Xiangqin Jiere Granules (XQJRG), a Chinese patent medicine used to treat acute fever in children caused by colds, seasonal flu, and … sequently, the colonic contents of rats were analyzed using 16S rRNA gene sequencing and untargeted metabolomics. The short-chain fatty acid content was quantified using high-throughput targeted metabolomics. The expression of short-chain fatty acid receptors and pro-inflammatory genes in the colonic tissue was evaluated using quantitative real-time PCR, Weste …

Metabolomics reveals changes in levels of fecal branched chain amino acids and organic acids in very preterm infants fed human milk fortified with bovine colostrum.

BACKGROUND & AIMS: Human milk is the optimal diet for very preterm infants (VPIs), but it requires nutrient fortification to support growth. Bovine colostrum (BC), rich in intact proteins and bioactive components, could serve as a novel fortifier with potential benefits to VPIs gut health. To evaluate a possible effect of feeding BC on intestinal metabolism, the gut microbiota, and their interaction, we studied the fecal metabolome of VPIs in the first month of life, as compared with a conventional fortifier (CF, based on infant formula ingredients). METHODS: Fecal samples were collected from

Mechanism of Panax notoginseng saponins in improving cognitive impairment induced by chronic sleep deprivation based on the integrative analysis of serum metabolomics and network pharmacology.

ETHNOPHARMACOLOGICAL RELEVANCE: Panax notoginseng saponin (PNS) has a variety of biological activities, such as improvement of myocardial is … a cognitively impaired rat model, and explored the mechanism of action of PNS by integrating serum metabolomics and network pharmacology, which was further verified by molecular docking and experiments. RESULTS: The results showed that PNS significantly shortened the escape latency, increased the target quadrant time and the number of traversing platforms, a …

Integrating 2D NMR-based metabolomics and in vitro assays to explore the potential viability of cultivated Ophiocordyceps sinensis as an alternative to the wild counterpart.

Ophiocordyceps sinensis is widely used to treat various diseases and as a health supplement. The present study comprehensively compared the metabolic differences between wild and cultivated O. sinensis through 2D 1H-13C HSQC-based metabolomics, and assessed their anti-lung cancer activity on A549 cells. To characterize the global metabolic profile, sample preparation was scrutinously optimized, and both polar (1:4 methanol-water) and non-polar (1:4 methanol-chloroform) extracts of O. sinensis were investigated. A total of 47 and 10 metabolites were identified in the polar and non-polar extract…

Mechanisms of exacerbation of Th2-mediated eosinophilic allergic asthma induced by plastic pollution derivatives (PPD): A molecular toxicological study involving lung cell ferroptosis and metabolomics.

Polystyrene microplastics (PS-MP) and dibutyl phthalate (DBP) are plastic pollution derivatives (PPDs) commonly found in the natural environ … at PPD aggravated eosinophilic allergic asthma in mice. The mitochondrial morphological changes and metabolomics of mice confirmed that ferrotosis and oxidative stress played key roles in this process. Treatment with 100 mg/Kg deferoxamine (DFO) provided significant relief, and metabolomic analysis of lung tissue supported the molecular toxicological. Our find …

Based on Serum Pharmacochemistry and Metabolomics Studied the Pharmacodynamic Material Basis and Mechanism of Rubi Fructus (Fupenzi) in Improving the Symptom of Kidney-Yang Deficiency.

Rubi fructus (Fupenzi) is the immature fruit of East China Rubi fructus, which is widely used in medicine, food, health food and other fields. Since ancient times, Fupenzi has been considered to be an important medicine for tonifying the kidney in terms of nourishing the liver and kidney, fixing essence and reducing urine, but its effective components and mechanism are not clear. In this paper, the effective components of Rubi fructus were analyzed by detecting the components of Fupenzi in vivo and in vitro. Adenine was used to replicate the model of kidney yang deficiency, and organ index, bi

Plasma metabolomics changes comparing daytime to overnight infusions of home parenteral nutrition in adult patients with short bowel syndrome: Secondary analysis of a clinical trial.

BACKGROUND: Home parenteral nutrition (HPN) is often cycled nocturnally and is expected to result in glucose intolerance and sleep disruptio … sing metabolic pathways previously implicated in circadian rhythms. As this is the first untargeted metabolomics study of HPN, larger studies are needed.

Widely targeted metabolomics combined with E-tongue and E-nose reveal dynamic changes of tender coconut water in responses to the infection of Ceratocystis paradoxa.

Ceratocystis paradoxa is a major cause of postharvest disease in tender coconuts worldwide. We conducted a comprehensive study using widely targeted metabolomics, electronic tongue (E-tongue), and electronic nose (E-nose) analyses to investigate the impacts of C. paradoxa invasion on the quality of tender coconut water (TCW) from fresh control (FC), uninoculated (UN), skin-inoculated (SI), and deep-inoculated (DI) nuts. DI exhibited significantly higher taste indicators associated with bitterness, saltiness, astringency aftertaste, and bitter aftertaste, as well as odor sensor values related t…

Combined effects of microcystin-LR and rice straw-derived biochar on the hepatic antioxidant capacity of zebrafish: Insights from LC-MS/MS-based metabolomics analysis.

Microcystin-LR (MC-LR) produced by cyanobacteria blooms poses a serious risk to aquatic organisms. Rice straw-derived biochar (BC) is gradually being utilized as an effective adsorbent to remove water pollutants. In the present study, the combined toxicity of MC-LR and BC on hepatic antioxidant capacity and metabolic phenotype of zebrafish (Danio rerio) were conducted due to the increasing concern of eutrophication in aquatic environments. Female zebrafish were exposed to solutions of MC-LR (10 μg/L) and BC (100 μg/L) individually and in combination for 30 days. The results indicated that sub-

The role of acupuncture in women with advanced reproductive age undergoing in vitro fertilization-embryo transfer: A randomized controlled trial and follicular fluid metabolomics study.

BACKGROUND: The objective of this study was to determine the efficacy of acupuncture on the outcome of in vitro fertilization (IVF) in elderly infertile patients with kidney qi deficiency, and to explore its possible mechanism from the perspective of pseudo-targeted metabolomics of follicular fluid. METHODS: Sixty cases of elderly women undergoing IVF were sampled and randomly divided into 2 equal groups: the treatment and the elderly control (HA) group. In the treatment group, routine ovulation induction combined with acupuncture treatment was used. Routine ovulation induction combined with …