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High Fluorescent Lymphoid Cell Levels Assessed on Initial Blood Count in Newly Diagnosed Multiple Myeloma Carry Prognostic Significance.

The outcome of multiple myeloma (MM) has tremendously improved over the past decade, due to the development of efficient chemotherapy and mo … d cell analysers now include fluorescent staining, allowing to identify cells with higher levels or nucleic acids such as activated B-lymphocytes/plasma cells with highly active ribosomal activity related to antibody production. Here, a prospective evaluation of peripheral high fluorescent lymphoid cell (HFLC) levels was carried out on samples from patients wit …

Nanomodulation of blood-labyrinth barrier enhances neuroprotection and antioxidant intervention for noise-induced hearing loss.

The treatment of noise-induced hearing loss (NIHL) is challenged by the blood-labyrinth barrier (BLB), which impedes effective drug delivery to the cochlea. NIHL serves as a model for sensorineural hearing loss (SNHL), highlighting the urgent need for advanced therapeutic strategies that can traverse BLB and target cochlear pathology. Here, we report the development of tFNA-RA@G-Ang2, a multi-stage, biomimetic nanocomposite designed for targeted inner ear therapy. This platform integrates tetrahedral framework nucleic acids (tFNA) to enhance cellular internalization, gelatin nanoparticles (GNP…

Novel quantitative and specific RT-qPCR assay for UK subtypes of European strain of Tick-borne encephalitis virus.

Two closely related tick-borne Orthoflaviviruses have been detected in the UK; tick-borne encephalitis virus (TBEV) and louping ill virus (LIV). Diagnosis of tick-borne encephalitis is achieved by detection of IgM/IgG antibodies using ELISA, Immunofluorescence, or by RT-PCR. High sequence and structural similarities between these viruses means they are hard to distinguish due to high cross-reactivity. We have developed a novel RT-qPCR assay using recently identified subtypes of TBEV in the UK and a probe incorporating locked nucleic acids, strengthening hybridisation between the probe and targ…

Prevalence, Species Characterization, and Genetic Diversity of Bartonella Infections in Rodents From Mudflat Wetlands Along the Eastern Coast of Jiangsu Province in China.

Objective: To investigate the infection status, species composition, and genetic diversity of Bartonella in local rodent populations in coastal mudflat wetland habitats in eastern Jiangsu Province of China. Methods: From March to June 2023, rodents were captured in mudflat wetlands of Dongtai and Tinghu Counties, Eastern China. Rodent species were identified, and nucleic acids were extracted from liver and spleen tissues. The mitochondrial cytochrome b (mt-cytb) gene was amplified by PCR, while Bartonella-specific citrate synthase (gltA) and 16S rRNA genes were amplified by semi-nested PCR. Ph…

First report on ATR-FTIR and machine learning-based sputum profiling for TB detection: A compendium of potential biomarkers.

Tuberculosis (TB) remains a significant global health issue, with millions of new cases reported annually and persistent limitations in conv … omising potential in establishing disease status. Peaks corresponding to carbohydrates, lipids, and nucleic acids further show the biochemical divergence between the two groups. The observed spectral variations demonstrate a clear potential for sputum-based differentiation of TB using vibrational spectroscopy. These findings highlight the potential for reagent- …

Улучшения в внеклеточной терапии для нейродегенеративных заболеваний.

Нейродегенеративные заболевания представляют собой серьезную и растущую проблему для общественного здравоохранения во всем мире. Нынешние терапевтические стратегии часто не позволяют остановить или обратить вспять прогресс в области заболеваний, что подчеркивает настоятельную необходимость применения новых подходов. Внеклеточные везикла (ЭМ) привлекают внимание в качестве потенциальных терапевтических агентов в силу их роли в межклеточной связи и их способности перевозить биоактивные грузы, включая белки, нуклеиновые кислоты и липиды. В этом обзоре содержится всеобъемлющий обзор биологии ЭМ, и

Совпадение в аннотации оригинала
Neurodegenerative diseases represent a significant and growing challenge to public health worldwide. Current therapeutic strategies often fa … in intercellular communication and their ability to transport bioactive cargo, including proteins, nucleic acids, and lipids. This review provides a comprehensive overview of the biology of EVs, their involvement in neurodegenerative diseases, and the potential for EV-based therapies. We discuss the different types of EVs, their biogenesis, and their cargo com …

Понятия о вертильной роли внеклеточных везиков в лечении нарушений CNS.

E xtracellular vesicles (EVs) представляют собой липидные нанокарьеры, скрытые липидом, состоящие главным образом из фосфолипидов и мембранных белков. Они высвобождаются клетками в окружающих внеклеточной среде и различаются по размерам, составу и биогенезам. Помимо своей естественной роли в межклеточном общении, выступая посредником в передаче белков, липидов и нуклеиновых кислот (таких, как mRNA и miRNA) между клетками, ЭМ стали весьма разносторонней и перспективной терапевтической платформой для целого ряда сложных расстройств, особенно тех, которые влияют на центральную нервную систему (CN

Совпадение в аннотации оригинала
E xtracellular vesicles (EVs) are lipid bilayer-enclosed nanocarriers composed primarily of phospholipids and membrane proteins. They are re … their natural role in intercellular communication, mediating the transfer of proteins, lipids, and nucleic acids (like mRNA and miRNA) between cells, EVs have emerged as a highly versatile and promising therapeutic platform for a range of challenging disorders, particularly those affecting the central nervous system (CNS) and various cancers. The CNS presents …

Intraoperative rapid assessment of the deep muscle surgical margin of tongue squamous cell carcinoma via Raman spectroscopy.

PURPOSE: An accurate assessment of the surgical margins of tongue squamous cell carcinoma (TSCC), especially the deep muscle tissue, can hel … an analysis showed that TSCC tissues contained significantly higher levels of proteins, lipids, and nucleic acids compared to the adjacent normal tissues. In addition, we have also explored the potential of Raman spectroscopy in classifying different histological grades of TSCC. CONCLUSION: The PCA-LDA tissue classification model based on Raman spectroscopy ex …

Backbone 1H, 13C, and 15N chemical shift assignments for human SERF2.

Human small EDRK-rich factor protein SERF2 is a cellular driver of protein amyloid formation, a process that has been linked to neurodegenerative diseases including Alzheimer's and Parkinson's disease. SERF2 is a 59 amino acid protein, highly charged, and well conserved whose structure and physiological function is unclear. SERF family proteins including human SERF2 have shown a tendency to form fuzzy complexes with misfolded proteins such as α-Synuclein which has been linked to Parkinson's disease. SERF family proteins have been recently identified to bind nucleic acids, but the binding mecha…

Minimal Determinants for Lifelong Antiviral Antibody Responses in Mice from a Single Exposure to Virus-like Immunogens at Low Doses.

The durability of an antibody (Ab) response is highly important for antiviral vaccines. However, due to the complex compositions of natural … e is sufficient to induce a long-lived neutralizing Ab (nAb) response. The introduction of internal nucleic acids dramatically modulates the magnitude of Ab responses without an alteration of the long-term kinetic trends. These Abs are characterized by very slow off-rates of ~0.0005 s-1, which emerged as early as day 5 after injection and these off-rates are co …

Multiarmed DNA jumper and metal-organic frameworks-functionalized paper-based bioplatform for small extracellular vesicle-derived miRNAs assay.

Small extracellular vesicle-derived microRNAs (sEV-miRNAs) have emerged as promising noninvasive biomarkers for early cancer diagnosis. Here … -type analyzer is promising for the fast, easy, low-cost, and highly sensitive detection of various nucleic acids and their mutation derivatives, making it ideal for POC biosensing.

Toxicologic Pathology Forum: Apoptosis/Single Cell Necrosis as a Possible Procedural Effect in Primate Brain Following Ice-Cold Saline Perfusion.

Nonclinical studies of test articles (TAs) in nonhuman primates are often designed to assess both biodistribution and toxicity. For this purpose, studies commonly use intravenous perfusion of ice-cold (2°C-8°C) saline to facilitate measurements of TA-associated nucleic acids and proteins, after which tissues undergo later fixation by immersion for histological processing and microscopic evaluation. Intriguingly, minimal apoptosis/single cell necrosis (A/SCN) of randomly distributed neural cells is evident in the cerebral cortex and less often the hippocampus in animals from all groups, includi…

A New Paradigm in Spinal Cord Injury Therapy: from Cell-free Treatment to Engineering Modifications.

Spinal cord injury (SCI) is an intractable and poorly prognostic neurological disease, and current treatments are still unable to cure it completely and avoid sequelae. Extracellular vesicles (EVs), as important carriers of intercellular communication and pharmacological effects, are considered to be the most promising candidates for SCI therapy because of their low toxicity and immunogenicity, their ability to encapsulate endogenous bioactive molecules (e.g., proteins, lipids, and nucleic acids), and their ability to cross the blood-brain/cerebrospinal barriers. However, poor targeting, low r…

Exploring Splicing Modulation as an Innovative Approach to Combat Pancreatic Cancer: SF3B1 Emerges as a Prognostic Indicator and Therapeutic Target.

Pancreatic ductal adenocarcinoma (PDAC) poses significant challenges in terms of prognosis and treatment. Recent research has identified spl … es involved in regulation of gene expression, primarily related to mRNA splicing, and metabolism of nucleic acids. PDAC cultures were highly sensitive to the SF3B1 modulators, E7107 and Pladienolide-B, showing IC50s in the low nanomolar range. These compounds induced apoptosis, associated to induction of the MCL-1/S splice variant. and reduced cell migration, a …

Enhanced Skin Penetration and Efficacy: First and Second Generation Lipoidal Nanocarriers in Skin Cancer Therapy.

Skin carcinoma remains one of the most widespread forms of cancer, and its global impact continues to increase. Basal cell carcinoma, melano … unctional characteristics, these nanocarriers can deliver a range of medications, such as peptides, nucleic acids, and chemotherapeutics, via different biological barriers, such as the skin. In this review, an effort was made to present the mechanism of lipid nanocarrier permeation via cancerous skin. In addition, recent research advances in lipid nanocarriers …

Spontaneous tumor lysis syndrome (STLS) during biopsy for burkitt lymphoma: a case report.

BACKGROUND: Tumor lysis syndrome (TLS) is a hematologic oncological emergency characterized by metabolic and electrolyte imbalances. On breakdown of tumor cells, enormous amounts of potassium, phosphate, and nucleic acids are released into systemic circulation. TLS mainly occurs during chemotherapy. However, there are rare incidences of spontaneous tumor lysis syndrome (STLS) prior to commencement of therapy. CASE PRESENTATION: In the case being reported, the child had just undergone a biopsy. As the incision was being closed, there was a sudden onset of high fever, arrhythmia, severe hyperka…

Rapid Cryptococcus electroporated-lysis and sensitive detection on a miniaturized platform.

Fast and accurate detection of Cryptococcus and precise differentiation of its subtypes is of great significance in protecting people from c … ell lysis (<30 s) and an electrochemical detection (ED) zone for sensitive analysis of the released nucleic acids. The EL zone adopted a design of microelectrode arrays, which obtains a large electric field intensity at the constriction of the microchannel, addressing the safety concerns associated with high-voltage lysis. The device enables a limit of detectio …

Potential therapeutic application and mechanism of gut microbiota-derived extracellular vesicles in polycystic ovary syndrome.

Polycystic ovary syndrome (PCOS) is a prevalent endocrine and metabolic disorder affecting women of reproductive age. The syndrome is charac … secreted by the gut microbiota and contain a variety of components, including proteins, lipids and nucleic acids. They serve as signaling molecules, facilitating bacterial-bacterial and bacterial-host communications. Bacterial extracellular vesicles (BEVs) affect host cells through the delivery of bioactive substances and physical interaction through membrane …

The 2023 South Sudanese outbreak of Hepatitis E emphasizes ongoing circulation of genotype 1 in North, Central, and East Africa.

In April 2023, an outbreak of acute hepatitis was reported amongst internally displaced persons in the Nazareth community of South Sudan. IgM serology-based screening suggested the likely etiologic agent to be Hepatitis E virus (HEV). In this study, plasma specimens collected from anti-HEV IgM-positive cases were subjected to additional RT-qPCR testing and sequencing of extracted nucleic acids, resulting in the recovery of five full and eight partial HEV genomes. Maximum likelihood phylogenetic reconstruction confirmed the genomes belong to HEV genotype 1. Using distance-based methods, we show…

Screening of urinary and genital tracts of adult females for the Chlamydia-like bacterium Parachlamydia acanthamoebae.

Some members of the Chlamydiales order, particularly Chlamydia, Waddlia, and Parachlamydia, have been associated with adverse pregnancy outc … nced miscarriage. Clinical samples were tested for Parachlamydia DNA by species-specific PCR assay. Nucleic acids of P. acanthamoebae were not detected in any endocervical, vaginal, and urine specimens tested. This investigation could not bring evidence for the existence of Parachlamydia in the urogenitalia of the female cohort tested. The role of Parachlamydia …