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Molecular Landscape in Pediatric and Young Adult Thyroid Cancer: A Brazilian Cohort Study.

Thyroid carcinoma in children and adolescents displays distinct molecular features compared with adult disease, with gene fusions playing a … results was observed, likely reflecting technical limitations related to the use of formalin-fixed, paraffin-embedded tissue. In conclusion, RET fusions were relatively uncommon in this Brazilian cohort but were enriched in younger patients, underscoring age-related differences in the molecular landscape of pediatric thyroid carcinoma and highlighting the …

Establishment and clinical performance evaluation of the thyroglobulin colloidal gold immunochromatographic assay.

BACKGROUND: Lymph node metastasis increases recurrence risk in differentiated thyroid carcinoma (DTC). To address limitations of conventiona … GICA) and traditional Roche electrochemiluminescence immunoassay (ECLIA) methods, respectively. The paraffin pathology results served as the gold standard. RESULTS: The CGICA method demonstrated comparable performance to the Roche ECLIA method in serum sample detection, as evidenced by excellent correlation in regression analysis (r=0.996) and Bland-Altma …

A gas-pressurized coaxial tubular flow-loop apparatus for module-scale barocaloric heat transfer measurements.

Barocaloric materials offer a promising route toward solid-state cooling and pressure-tunable thermal energy storage, but most experimental … ansfer channel, thereby decoupling pressure delivery from thermal exchange. Using microencapsulated paraffin as a model pressure-tunable phase change material, we demonstrate two complementary measurement modes based on temperature scanning and step-temperature operation. Temperature-scanning measurements reproduce the bimodal solid-solid and solid-liquid …

Mosaic switch for PAM-free and one-pot CRISPR/Cas12a detection.

CRISPR/Cas12a combined with nucleic acid amplification enables highly specific and sensitive detection. However, its broader deployment is c … ain single-nucleotide discrimination. Lyophilizing mosaic switch reagents and encapsulating them in paraffin improve stability and usability, which also enables straightforward one-pot integration with recombinase polymerase amplification (RPA) via a solid-liquid phase transition. Applied directly to 58 extraction-free mpox clinical samples, this platform …

Detecting Rare ALK Gene Fusions in Unclassified Spindle Cell Lung Tumors Using Anchored Multiplex PCR/Targeted RNA Next-Generation Sequencing.

Pulmonary spindle cell tumors are aggressive neoplasms with limited systemic treatment options, although a subset may harbor actionable genomic alterations. Because conventional fusion assays may miss rearrangements involving atypical or previously uncharacterized partners, we systematically investigated oncogenic fusions in pulmonary spindle cell tumors using anchored multiplex PCR-based targeted RNA sequencing. Formalin-fixed, paraffin-embedded tumor samples from 11 surgically resected pulmonary spindle cell tumors, excluding metastatic sarcomas, were analyzed using the FusionPlex Sarcoma pa…

Modulating Photons and Phonons in Graphene-Based Phase Change Materials for Solar-Thermal-Electrical Generation.

Solar-thermal-electrical generators offer a promising route for the conversion of solar energy into electricity. Their reliability can be en … F-derived uniformly dispersed cobalt nanoparticles within rGO framework. After the encapsulation of paraffin wax (PW), the resultant rGO@Co/C-PW composite PCMs demonstrate a remarkable solar-thermal conversion efficiency of 92.5% under 100 mW·cm-2 irradiation, attributed to the synergistic interplay between the broadband absorption and non-radiative relaxa …

Somatic Cancer Driver Mutation Analysis in Endometriosis with Tumor-like Presentations: A Case Series Study.

Background/Objectives: Endometriosis manifests as ectopic endometrial tissue outside the uterine cavity. This lesion can sometimes appear at … used to isolate epithelial cells from endometriotic glands in tissue sections from formalin-fixed, paraffin-embedded blocks, and the microdissected epithelium was analyzed for cancer driver mutations. Results: Unlike conventional endometriosis, these tumor-like lesions were generally sizable and clinically presented in the groin area, aortic wall, omentum …

Superhydrophobic, recyclable bacterial cellulose/graphene oxide composite aerogels with enhanced mechanical and thermal stability for efficient oil-water separation.

The oil pollution in aquatic ecosystems poses a serious threat to public health and ecological balance. In addressing this challenge, this i … retaining 85.2% of ethanol adsorption capacity after 20 extraction cycles, and preserving 76.7% of paraffin oil adsorption capacity. These results collectively demonstrate the broad application prospects of this bio-based oil-absorbing material in oil-water separation technologies.

The role of rapid IHC in elevating STAS diagnostic accuracy during intraoperative frozen section for lung adenocarcinoma.

AIM: The aim of this study was to evaluate whether the utility of cytokeratin (AE1/AE3) rapid immunohistochemistry (IHC) in combination with haematoxylin-eosin (H&E) staining improves the intraoperative diagnosis of spread through air spaces (STAS) on frozen sections (FS). METHODS AND RESULTS: This study included 153 patients. Three pathologists independently evaluated STAS on FS using either HE staining alone or in combination with rapid IHC, with postoperative paraffin sections serving as the gold standard. Sensitivity and specificity were compared, along with interobserver and intraobserve…

Comparative evaluation of HTG and TempO Seq targeted transcriptome profiling methods.

Transcriptomic profiling is a cornerstone of molecular biology and plays a central role in translational and molecular oncology, where reliable gene expression measurements are essential. Targeted RNA sequencing approaches offer several advantages over whole-transcriptome methods, including high sensitivity, reproducibility, and reduced turnaround time, particularly when working with formalin-fixed paraffin-embedded (FFPE) samples. HTG EdgeSeq HTP has previously addressed these needs; however, the recent discontinuation of this platform has created a technical gap for targeted transcriptome an…

Increased Expression of ProBDNF/Sortilin in Localized Prostate Cancer Tissues Compared to Adjacent Non-Cancerous Tissues.

BACKGROUND: The unprocessed precursor of brain-derived neurotrophic factor (BDNF), proBDNF, has emerged as a potential determinant of therap … ls of proBDNF, sortilin, and p75NTR, we performed immunohistochemical analyses on 18 formalin-fixed paraffin-embedded (FFPE) PCa tissues obtained at radical prostatectomy between 2024 and 2025, together with matched para-carcinoma tissues. RESULTS: Compared with para-carcinoma tissues, immunohistochemistry in 18 paired specimens showed that pro-BDNF was s …

Low frequency of MLLT10 risk SNPs in Korean meningiomas: an exploratory analysis highlighting population-specific differences.

INTRODUCTION: Several MLLT10-associated single-nucleotide polymorphisms (SNPs) have been identified by genome-wide association studies (GWAS … ars, comprising 62 fresh-frozen tissues collected during 1999-2003 (Period 1) and 81 formalin-fixed paraffin-embedded tissues from 2006-2023 (Period 2). RESULTS: Three SNPs were detected in 9 of 143 meningiomas (6.3%). While the differences did not reach statistical significance (p > 0.05), minor allele frequencies of all three SNPs were reduced two- to f …

Differences in collagen structure and organization in primary and metastatic pancreatic cancer and the effects of FOLFIRINOX treatment.

Pancreatic ductal adenocarcinoma (PDAC) is defined by a dense, collagen-rich stroma that limits therapeutic efficacy and drives metastasis. While collagen reorganization has been studied in resected PDAC tumors, its structure and organization in metastatic PDAC and response to chemotherapy remains poorly characterized. We evaluated collagen structure by Second Harmonic Generation (SHG) imaging of formalin fixed paraffin embedded tissue from pancreatic tumors and liver metastases of 20 PDAC patients that were untreated or treated with FOLFOX or FOLFIRINOX (FOL). Collagen fiber width, length, al…

Histopathological assessment to detect colorectal ovarian micrometastasis following prophylactic salpingo-oophorectomy in postmenopausal patients.

INTRODUCTION: Colorectal cancer (CRC) is one of the most prevalent malignancies and often leads to metastatic disease. Ovarian metastasis oc … mour resection and concurrent PSO were conducted retrospectively. Tissue blocks with formalin fixed paraffin embedded ovarian tissue were sectioned at five levels, and both routine histopathological and additional immunohistochemical staining for CK20 and CDx2 were performed. The primary outcome measure was the incidence of CRC micrometastases (%). RESULT …

Создание нового банка тканей нервной системы человека: Дебрецинский университет — Введение и ретроспективный анализ данных

Резюме тканей мозга человека, полученных в результате вскрытия, а также мышечных и нервно-паралитических образцов, полученных в результате биопсии, представляют собой необходимые ресурсы для изучения патомеханизма нервно-психических расстройств. В этом исследовании мы представляем " Нервусовый системный тканевый банк " Университета Дебрецена, который содержит формализованные и формализованные образцы головного мозга с парафинами (FFPE), а также образцы замороженного нерва и мышечной биопсии. Была создана база данных, содержащая клинические и патологические параметры. В случае вскрыти

Совпадение в аннотации оригинала
Abstract Human brain tissues obtained from autopsies, as well as muscle and nerve samples derived from biopsies, constitute indispensable re … System Tissue Bank of University of Debrecen, which contains formalin-preserved and formalin-fixed paraffin-embedded (FFPE) brain samples, as well as frozen nerve and muscle biopsy specimens. Database was established containing clinical and pathological parameters. For autopsy cases, the following variables were recorded: sex, age, number of hospitalizati …

Re-evaluating archival specimens as a pathologic diagnostic resource for neuronal intranuclear inclusion disease.

OBJECTIVES: Neuronal intranuclear inclusion disease (NIID) is a multisystem neurodegenerative disorder caused by GGC repeat expansions in the NOTCH2NLC gene. Although genetic testing has improved diagnostic efficiency, histopathologic confirmation remains essential. We investigated whether routine archival surgical specimens could serve as an alternative pathologic resource for NIID diagnosis. METHODS: We retrospectively analyzed archival formalin-fixed, paraffin-embedded specimens from patients with genetically confirmed NIID, including gastric antrum, colon, gallbladder, prostate, and kidne…

Prognostic Impact of CD57⁺ Natural Killer Cells and CD138⁺ Plasma Cells as Minor Components of the Tumor Microenvironment in Mixed Cellularity Classical Hodgkin Lymphoma.

The tumor microenvironment (TME) plays a crucial role in tumor progression and treatment response of classical Hodgkin lymphoma (CHL). Altho … ted on 50 cases of CHL of the mixed cellularity subtype. Specimens were obtained as formalin-fixed, paraffin-embedded (FFPE) blocks from the Pathology Department of the South Egypt Cancer Institute, Assiut University. Immunohistochemical staining for CD57 and CD138 was performed to evaluate NK cells and plasma cells within the TME. A tumor immune profiling …

Thin-Adipose Compartment at the Colonic Mesentery-Perirenal Fat Interface: Histological and Three-Dimensional Morphological Studies.

OBJECTIVES: To elucidate the anatomical characteristics and three-dimensional continuity of a previously unrecognized thin-adipose compartment between the colonic mesentery and retroperitoneum, using correlative microscopy and block-face imaging. METHODS: In this study, which was conducted at the anatomical laboratory of the Institute of Science Tokyo (formerly Tokyo Medical and Dental University), seven adult cadavers were examined. Histological analysis was conducted on six specimens using paraffin sections stained with Elastica van Gieson and Masson's trichrome. One cadaver underwent three…

Immunohistochemical Evaluation of GATA3 Expression in Patch-Stage Mycosis Fungoides (MF) Compared With Benign Inflammatory Dermatoses (BID).

The histopathologic distinction between patch-stage mycosis fungoides (MF) and benign inflammatory dermatoses (BID) remains a persistent diagnostic challenge, often due to overlapping clinical and immunophenotypic features. GATA binding protein 3 (GATA3), a transcription factor critical in T-helper 2 cell differentiation, has emerged as a potential immunohistochemical marker in T-cell neoplasms. This study aimed to evaluate GATA3 expression in patch-stage MF compared with BID to assess its diagnostic value. Sixty formalin-fixed, paraffin-embedded skin biopsies were retrospectively analyzed, in…

AKT3 and α-SMA in oral submucous fibrosis and oral squamous cell carcinoma with concomitant oral submucous fibrosis as potential prognostic indicators.

PURPOSE: Oral squamous cell carcinoma (OSCC) concomitant with oral submucous fibrosis (OSCC-OSMF) is a clinico-pathologically distinct entit … d correlated with related clinicopathological parameters. MATERIALS AND METHODS: 70 formalin-fixed paraffin-embedded tissue samples of histopathologically confirmed cases, including 10 NOM, 30 OSMF, and 30 OSCC-OSMF, were immunohistochemically stained with AKT3 and (α-SMA) antibodies to quantify MFs and myofibroblastic cancer associated myofibroblasts (my …