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In vitro comparison of the cytotoxicity of twelve endodontic materials using a new technique.

An in vitro method for the cytotoxicity testing of endodontic materials is described which aims to simulate the clinical situation. Materials can be tested in the presence or absence of a compacted layer of dentine chips mimicking the periapical dentine plug. A total of twelve materials were tested. In the absence of dentine, Kloroperka, Biocalex, Diaket and Endomethasone were slightly cytotoxic; AH26 with and without silver, Sealapex, Tubliseal and Kerr's pulp canal sealer were moderately cytotoxic, while Forfenan, Spad and Kri paste were strongly cytotoxic. In the presence of dentine the cyt

Quality Improvement Analysis of Operative Regimen on Urinary Tract Infections.

IMPORTANCE: Acute urinary tract infections (UTIs) are common after prolapse and anti-incontinence surgery, with incidence rates varying from … are for prophylactic intraoperative antibiotics. Patients discharged with a Foley catheter received methenamine hippurate and cranberry tablets per our standard practice. The primary outcome, incidence of symptomatic UTIs within 30 days postoperatively, was determined for pre-bundle and post-bundle implementation participants. Secondary analyses included the …

Comparative tissue tolerance of a new endodontic sealer.

An experimental radiopaque resin root canal sealer was tested for tissue biocompatibility in rat connective tissue. The controls were four widely used sealers: Sealapex, Kerr's sealer, AH-26, and Roth's sealer. Polyethylene tubes were filled with the sealers and allowed to set for 24 hours in 100% humidity. Each sealer was implanted in the connective tissue of Sprague-Dawley rats and removed after 3, 10, 20, 30, and 60 days, fixed, and histologically prepared for light microscope evaluation. At each time period there was practically no difference in the reaction of all the sealers. By 60 days,

[Pnemocystis jiroveci pneumonia: Comparison between conventional PCR and staining techniques].

Diagnosis of pneumocystis pneumonia is usually based on clinical features and X-rays photography and confirmed in the laboratory by visualisation of Pneumocystis organisms in stained preparations of respiratory specimens using several techniques (Gomori-Grocott, May-Grünwald Giemsa, bleu de toluidine O). Actually, PCR has considerably increased sensitivity of detection of Pneumocystis. The aim of this study is to compare conventional PCR results to those of staining techniques (Gomori-Grocott, May-Grünwald Giemsa) in addition to the X-ray and clinical findings in order to evaluate the contribu

The sealing ability of an epoxy resin root canal sealer used with five gutta-percha obturation techniques.

The purpose of the present study was to evaluate the apical sealing ability of an epoxy resin root canal sealer (AH-26) when used with different gutta-percha obturation techniques (i.e. cold lateral condensation, warm vertical condensation, hybrid condensation) and with Thermafil and Soft-Core obturators. Straight single-rooted teeth with mature apices were prepared according to the crown-down/step-back technique and divided in five groups of 45 teeth and one group of 12 control teeth. After root canal filling 15 teeth of each group were kept for 1 day, another 15 teeth for 1 week, and another

Characteristics and Prognostic Factors of Non-HIV Immunocompromised Patients With Pneumocystis Pneumonia Diagnosed by Metagenomics Next-Generation Sequencing.

OBJECTIVE: The aim of this study was to evaluate the potential of metagenomic next-generation sequencing (mNGS) for the diagnosis of pneumoc … ar lavage (BAL) specimens in forty-six patients with non-HIV PCP, compared to only 15.2% for Gomori Methenamine silver (GMS) staining and 84.8% for Serum 1,3-beta-D-glucan (BDG). Among them, the mean age was 46.4-year-old (range 18-79-year-old) and mortality rate was 43.5%. The dominant underlying conditions were connective tissue diseases (34.8%), autoimmune …

UTIs in patients with neurogenic bladder.

Urinary tract infections (UTI) remain one of the most prevalent and frustrating morbidities for neurogenic bladder patients, and death attributed to urosepsis in the spinal cord injury (SCI) patient is higher when compared to the general population. Risk factors include urinary stasis, high bladder pressures, bladder stones, and catheter use. While classic symptoms of UTI include dysuria, increased frequency and urgency, neurogenic bladder patients present differently with increased spasticity, autonomic dysreflexia, urinary incontinence, and vague pains. Multiple modalities have been assessed

Glucuronoxylomannan in the Cryptococcus species capsule as a target for Chimeric Antigen Receptor T-cell therapy.

BACKGROUND AIMS: The genus Cryptococcus comprises two major fungal species that cause clinical infections in humans: Cryptococcus gattii and … transduced control T cells (NoDNA T cells). Moreover, lung tissue sections were stained with Gomori Methenamine Silver and evaluated by NanoZoomer (Hamamatsu), revealing a significantly lower number of titan cells, with perimeters ranging from 50 to 130 µm and giant cells >130 µm in the CAR T-cell treated group when compared with other groups. Therefore, the …

Cutaneous alternariosis in a patient with idiopathic pulmonary fibrosis.

A 78-year-old farmer presented with symptomless skin lesions for evaluation. Two years prior, he had developed idiopathic pulmonary fibrosis … lear leukocytes were seen in the adjacent epidermis. Periodic acid-Schiff (PAS) and Gomori's silver methenamine stains showed a multitude of broad branching fungal hyphae and large spores within the aforementioned cavity, both free and within the cytoplasm of giant cells (Fig. 2). Immunohistochemistry was performed by means of the alkaline phosphatase anti-al …

Inflammatory Bowel Disease-Associated Colorectal Cancer: Translational and Transformational Risks Posed by Exogenous Free Hemoglobin Alpha Chain, A By-Product of Extravasated Erythrocyte Macrophage Erythrophagocytosis.

A 33-year-old Hispanic woman with newly diagnosed human immunodeficiency virus (HIV) infection, a CD4 T-lymphocyte count of 2, viral load of … budding yeast forms, predominantly within histiocytes, with few organisms residing extracellularly. Methenamine silver stain confirmed the presence of 2-4 microm fungal spores suggestive of Histoplasma capsulatum (Fig. 2). Because of the patient's deteriorating condition, intravenous amphotericin B was initiated after tissue culture was obtained. Within the f …

Antimicrobial activity of four root canal sealers against endodontic pathogens.

The antibacterial effects of various types of widely used endodontic sealers have not been compared systematically on facultative or obligate anaerobic endodontic pathogens. The aim of this study was to evaluate the antimicrobial properties of four commonly used endodontic sealers: two epoxy-resin-based sealers (AH26, AH plus), one zinc-oxide eugenol-based sealer (N2), and one calcium hydroxide-based sealer (Sealapex). The testing microbes were four facultative anaerobic species (Streptococcus mutans, Streptococcus sanguis, Escherichia coli, and Staphylococcus aureus) and four obligate anaerob

The apical seal of root canal sealing cements using a radionuclide detection technique.

The aim of this study was to evaluate the apical seal obtained with various root canal sealing cements using a new method based on the use of a radionuclide and an external detection technique. A total of 150 maxillary incisors and canines, which had been kept in saline solution, were used. The root canals were instrumented with a stepback technique and circumferential filing. Six experimental groups and two control groups were randomly formed. Each experimental group was obturated by a lateral condensation technique with gutta-percha points and a different sealing cement (two based on zinc ox

[Expression and role of soluble epoxide hydrolase in renal tissue of two kidneys one clip hypertension rats model].

OBJECTIVE: To investigate renal expression of soluble epoxide hydrolase (sEH) in 2-kidney-1-clip rats and explore the role of sEH in renal a … y and Western blotting. Histology was analysed after kidney sections were stained by Grocott-Gomori methenamine-silver nitrate. RESULTS: After surgery, the systolic blood pressure in 2K1C group gradually became higher than that in sham group. Urine protein excretion was statistically increased in 2K1C group on the 30 th and 40 th days, while serum sodium was …

Propantheline bromide in the management of hyperhidrosis associated with spinal cord injury.

OBJECTIVE: To report 2 cases in which oral propantheline reduced the discomfort associated with sweating related to spinal cord injury (SCI) … there have been attempt at other systemic therapy using mecamylamine, atropine, propoxyphenel, and methenamine. Scopolamine patches also have been used successfully in a small number of patients. Other agents that have been used include dibenamine, piperoxan, and phentolamine. Systemic propantheline also has been listed as an agent with potential efficacy in …

Disseminated pheohyphomycosis.

A 40-year-old male agricultural laborer presented to our clinic with asymptomatic swellings on his left hand and left leg of 2 years' durati … sections. The fungal elements were found within the cystic cavity on special staining with Gomori's methenamine silver (GMS) and Masson-Fontana stains. The hyphae had irregularly placed branches and showed constrictions around their septae, thus resembling pseudohyphae and yeast forms (Fig. 3). Fine needle aspiration cytology from one of the swellings showed …

Effect of ultrasonic vibration and various sealer and cement combinations on titanium post removal.

The purpose of this study was to determine the effect of ultrasonic vibration on the force required to remove prefabricated posts. Ninety-six extracted human canines were divided into eight groups, which were prepared and obturated with gutta-percha and either a eugenol-containing (Roth's 801 Elite) or eugenol-free (AH26) sealer. Titanium #6 Parapost XH posts were cemented with either zinc phosphate or Panavia 21 resin cement. One half of the sample was subjected to ultrasonic vibration for 16 min at the post-dentin interface, whereas the other half received no vibration (controls). Each combi

Effects of instrumentation, irrigation and dressing with calcium hydroxide on infection in pulpless teeth with periapical bone lesions.

AIM: The aim of this study was to evaluate the fate of microorganisms in root canals of teeth with infected pulps and periapical bone lesions with and without the use of calcium hydroxide medication. METHODOLOGY: Endodontic samples were cultured and microorganisms were counted and identified in 43 teeth before (sample 1) and after (sample 2) treatment during the first visit and before (sample 3) and after (sample 4) treatment during the second visit. In the first visit teeth were instrumented and half of the teeth were filled with a thick slurry of calcium hydroxide in sterile saline. The oth

Effect of root canal sealers and irrigation agents on retention of preformed posts luted with a resin cement.

This study examined the effect of three sealers and four irrigants on retention of Dentatus preformed posts luted with Panavia 21 resin cement. Gutta-percha with one of the following sealers--EWT, AH26, Nogenol, or no sealer (control)--was used to fill the root canals of 160 autoclaved incisor and premolar tooth roots. After 72 hr 8 mm post spaces were created with Dentatus Probos Pathfinders and Dentatus reamers. After irrigating the post spaces with saline, 5.25% NaOCl, 50% citric acid followed by NaOCl, or 40% H3PO4, followed by NaOCl, Panavia 21 TC cement was introduced into the canal on t

Cranberries for preventing urinary tract infections.

BackgroundCranberries have been used widely for several decades for the prevention and treatment of urinary tract infections (UTIs). This is … ared cranberry capsules and tablets. The comparison/control arms were placebo, no treatment, water, methenamine hippurate, antibiotics, or lactobacillus. Eleven studies were not included in the meta-analyses because either the design was a cross-over study and data were not reported separately for the first phase, or there was a lack of relevant data. Data in …

The Crystalline Nephropathies.

Crystalline nephropathies are a unique form of kidney disease characterized by the histologic finding of intrarenal crystal deposition. The intrinsic nature of some molecules and ions combined with a favorable tubular fluid physiology leads to crystal precipitation and deposition within the tubular lumens. Crystal deposition promotes kidney injury through tubular obstruction and both direct and indirect cytotoxicities. Further kidney injury develops from inflammation triggered by these crystals. From a clinical standpoint, the crystalline nephropathies are associated with abnormal urinalysis a

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… In contrast, the annular structures have weak rim staining on periodic acid–Schiff (PAS). 16 Silver-positive black staining of crystals is observed on Jones methenamine silver (JS), whereas strongly birefringent crystals are noted with polarization. 16 Intratubular crystal deposition is prevented by … Table 2 Crystalline nephropathies associated with dysproteinemias AKI, acute kidney injury; CKD, chronic kidney disease; EM, electron microscopy; ESKD, end-stage kidney disease; H&E, hematoxylin and eosin; HD, hemodialysis; IF, immunofluorescence; IVF, i.v. fluid; JS, Jones methenamine silver; LC, light …