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Proteome analysis of urinary biomarkers in a cigarette smoke-induced COPD rat model.

BackgroundChronic obstructive pulmonary disease (COPD) is a chronic inflammatory airway disease caused by inhalation of cigarette smoke (CS) and other harmful gases and particles.MethodsThis study aimed to explore potential urinary biomarkers for CS-induced COPD based on LC-MS/MS analysis.ResultsA total of 340 urinary proteins were identified, of which 79 were significantly changed (30, 31, and 37 at week 2, 4 and 8, respectively). GO annotation of the differential urinary proteins revealed that acute-phase response, response to organic cyclic compounds, complement activation classical pathway

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… Pulmonary function test and lung histopathology Pulmonary function was evaluated by the AniRes2005 animal lung function analysis system (Beijing Beilanbo Technology). … Funding National Natural Science Foundation of China (81973012, 82000881), National Key Research and Development Program of China (2018YFC0910202), Beijing cooperative construction project (110651103), Beijing Normal University (11100704). …

Effect of β2-microglobulin in evaluating the severity and prognosis of brain injury: a clinical study.

Backgroundβ2-microglobulin has been showing to be vital that associated with brain function and neurological diseases. This study aimed to explore the expressions of β2-microglobulin in blood and urine of the patients with brain injury, and the effect of hyperbaric oxygen therapy on the content of β2-microglobulin.MethodsThis prospective study included 54 patients with brain injury and 11 healthy controls. The patients were further assigned to two groups: the conscious disturbance group (n = 32) and the non-conscious disturbance group (n = 22) depending on the Glasgow Coma Scale (GCS). The pat

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… The concentrations of serum creatinine and blood urea nitrogen were detected with the kit (Beijing Jiuqiang Biotechnology Co., Ltd., Beijing, China). The whole procedure was operated strictly according to the instructions by experienced laboratory physicians. … Declarations Ethics approval and consent to participate This study was approved by the Ethics Committees of Beijing Chao-Yang Hospital of Capital Medical University and conformed to the Declaration of Helsinki guidelines. …

Investigation of the Underlying Mechanism of Sclerosteosis Expression in Muscle Tissue in Multiple Myeloma with Sarcopenia.

ObjectiveTo explore the role of sclerosteosis (SOST) gene expression in the occurrence and development of multiple myeloma (MM) complicated with sarcopenia.MethodsAnalysis of the SOST expression in skeletal muscle tissue of patients with MM using high-throughput sequencing combined with transcriptomics; observation of morphological changes of the mouse C2C12 myoblasts co-cultured with SP2/0 myeloma cells in Transwell; observation of the SOST expression in the C2C12 myoblasts using the immunofluorescence labeling method; and assessment of the changes in exercise capacity of mice with MM using e

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… Materials and Methods Experimental Objects Tissue Sample The paraffin-embedded skeletal muscle tissue samples were obtained from the Department of Orthopedics, Beijing Chaoyang Hospital, Capital Medical University. … All animal experiments were conducted according to the guidelines established by the National Institutes of Health ( http://grants1.nih.gov/grants/olaw/ ) and approved by Animal Use Committee of National Drug Clinical Trial Institution, Beijing Chao-yang Hospital. …

Progression-Free Survival of a Patient with Advanced Hepatocellular Carcinoma Treated with Adoptive Cell Therapy Using Natural Killer Cells: A Case Report.

Adoptive cell therapy (ACT) is a promising treatment that is considered safe and efficient. Natural killer (NK) cells play an important role in the innate immune system and destroy target cells such as tumor cells without prior sensitization. Here, we report a 59-year-old man with advanced diffuse hepatocellular carcinoma (HCC) who underwent 17 courses of NK cell treatment from March 2017 to July 2018. Although he presented with progressive disease, his hydrothorax and ascites decreased, and his state of mind, appetite and quality of life were markedly improved after treatment versus at admiss

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… NK Cell Preparation First, 500 μL NK activator (Clin-Biotechnology Co., Ltd., Beijing, China) was aspirated by a Pasteur pipette and added to a T-175 culture flask (Corning Incorporated, NY, USA). … NK cell culture medium (CTS TM AIM-V TM Serum-free Medium [Thermo Fisher Scientific, Waltham, MA, USA]; 5% plasma; 25 ng/mL recombinant human IL-15 [Beijing T&L Biotechnology Co., Ltd., Beijing, China]; 1000 U/mL recombinant human IL-2 [Shandong Jintai Biotechnology Co., Ltd., Jinan, China]) was prepared …

Impact of germline predisposition genes to hematologic malignancies on transplant outcomes and donor selection.

INTRODUCTION: Germline predisposition gene mutations may influence outcomes in patients undergoing hematopoietic stem cell transplantation (HSCT). METHODS: This retrospective study of 558 patients undergoing HSCT (allo-HSCT in 557 cases, auto-HSCT in 1 case) evaluated the impact of germline predisposition gene mutations on outcomes. RESULTS: Enrichment of HAVCR2 mutations in B-NHL and UNC13D mutations in MDS was associated with poorer prognosis. While homozygous mutation carriers (7.5%) overall did not have reduced survival, their outcomes were significantly better with unrelated versus rela

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… Methods Patients Between December 2017 and February 2024, a total of 558 patients underwent HSCT at Beijing Gobroad Boren Hospital (allo-HSCT in 557 cases, auto-HSCT in 1 case). The median age was 16.14 years. … Result Cohort characteristics This retrospective cohort included 558 patients with hematologic diseases who underwent HSCT at Beijing Gobroad Boren Hospital between December 2017 and February 2024. …

Консолидация, систематическая оценка и сопоставление руководящих рекомендаций в отношении лечения хронической боли: Протокол для составления карты рекомендаций в отношении цифрового хронического заболевания.

ВВЕДЕНИЕ: Хроническая боль 1 В 5 :: взрослые и дети во всем мире; однако управление попрежнему носит весьма различный характер, и, как правило, обеспечивается низкий уровень медицинского обслуживания. Несогласованность рекомендаций, содержащихся в руководящих принципах клинической практики в отношении лечения хронической боли, способствует неоптимальному лечению пациентов. Мы будем разрабатывать и распространять живую цифровую карту рекомендаций в отношении хронической боли (e-Chronic Bain RecMap) для выявления заслуживающих доверия рекомендаций в three высокоприоритетные области1) опиоиды,2)

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… DeGroote National Pain Centre (MGD NPC) Canada Life Cochrane Canada/GRADE center Cochrane Methods Equity Chinese GRADE center, Lanzhou University Beijing GRADE center Cochrane China World Health Organization (WHO) Collaborating Centre for Guideline Implementation and Knowledge Translation in China Guideline … University of Chinese Medicine, Beijing, China), Zachary Munn (Health Evidence Synthesis, Recommendations and Impact (HESRI), School of Public Health, Faculty of Health and Medical Science, The University of Adelaide, Adelaide, SA, Australia), Zoe Jordan (JBI, Faculty of Health and Medical Sciences, …

CRISPR application in hematological disorders: from bench to bedside.

AbstractClustered regularly interspaced short palindromic repeats (CRISPR)-associated protein genome editing has advanced from an experimental tool to a clinically validated therapeutic platform in hematology. Landmark successes in inherited blood diseases, including sickle cell disease, β-thalassemia, and severe combined immunodeficiency, have demonstrated that precise and durable genetic modifications can be safely and effectively implemented in human hematopoietic cells, positioning hematology at the forefront of translational genome editing. Beyond monogenic disease, CRISPR-based approache

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… We also acknowledge the statistical support provided by the Clinical Research Center of Beijing Chest Hospital. … J Beijing Univ Trad Chin Med . 2005;28(4):1–8. 14. Tang Y, Wang Y, Wang J, et al. Association between damp-heat constitution and gastrointestinal symptoms: a cross-sectional study. Chin Med . 2021;16(1):45. doi: 10.1186/s13020-021-00455-w 15. Song M, Chan AT. …

The deubiquitinase USP34 stabilizes SOX2 and induces cell survival and drug resistance in laryngeal squamous cell carcinoma.

Recent studies showed that the deubiquitinase ubiquitin-specific protease 34 (USP34) was involved in the tumorigenesis of several tumors, but its function and mechanism are still unclear in laryngeal squamous cell carcinoma (LSCC). In this study, we found that USP34 and SOX2 were elevated in LSCC tumor tissues, and we also found that USP34 expression was positively correlated with SOX2 expression. Our further studies showed that USP34 regulated the protein level of SOX2 in LSCC cells, but not the mRNA level, which suggested that USP34 stabilized SOX2. Moreover, USP34, as a deubiquitinase, coul

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… The paracancerous and tumor tissues of LSCC were collected from Beijing Hospital of Traditional Chinese Medicine, and the use of human tissues in this study was approved by the Institutional Review Board of Beijing Hospital of Traditional Chinese Medicine. … ACKNOWLEDGEMENTS The authors thank for the support from Beijing Hospital of Traditional Chinese Medicine. REFERENCES 1 Bray F , Ferlay J , Soerjomataram I , Siegel RL , Torre LA , Jemal A . …

Characteristics and Risk Factors of Ultra-High-Risk Patients with Newly Diagnosed Multiple Myeloma.

ObjectiveTo investigate the clinical characteristics and risk factors of ultra-high-risk (UHR) patients with newly diagnosed multiple myeloma (MM).MethodsWe screened UHR patients with a survival of less than 24 months and we selected patients with a concurrent survival of more than 24 months as a control group. We retrospectively analyzed the clinical characteristics of UHR patients with newly diagnosed MM and screened related risk factors.ResultsIn total we analyzed 477 patients, which included 121 (25.4%) UHR patients and 356 (74.6%) control patients. Median overall survival (OS) and progres

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… We screened newly diagnosed MM patients at Beijing Chaoyang Hospital, Capital Medical University, and analyzed the clinical characteristics and screened the prognostic factors of UHR MM patients. 2. Methods 2.1. … The study was approved by the Medical Ethics Committee of Beijing Chaoyang Hospital in accordance with the Declaration of Helsinki. 2.2. …

Prevalence, awareness, and treatment of anemia in Chinese patients with nondialysis chronic kidney disease: First multicenter, cross-sectional study.

This was the first multicenter, cross-sectional survey to assess the prevalence of anemia, patient awareness, and treatment status in China. Data of patients with chronic kidney disease (CKD; age, 18-75 years; both out- and inpatients) from 25 hospitals in Shanghai, seeking medical treatment at the nephrology department, were collected between July 1, 2012 and August 31, 2012. The prevalence, awareness, and treatment of anemia in patients with nondialysis CKD (ND-CKD) were assessed. Anemia was defined as serum hemoglobin (Hb) levels ≤12 g/dL in women and ≤13 g/dL in men. A total of 2420 patien

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… Similar findings were reported by DOPPS, according to which the target Hb achieved was low (39%–46%) in the 3 most developed metropolitan areas in China (Beijing, Shanghai, and Guangzhou), in addition to a low rate of intravenous iron use in patients undergoing hemodialysis: 41% in Beijing, 18% in Shanghai … BMC Nephrol 2014 ; 15 : 205 . 25528680 10.1186/1471-2369-15-205 PMC4382930 [35] Zhang L Zuo L Xu G Community-based screening for chronic kidney disease among populations older than 40 years in Beijing . …

Identification of Plasma Protein Biomarkers and Drug Targets for Hematologic Malignancies by Proteome-wide Mendelian Randomization.

Background: It has been reported that the proteome in blood was an important source for biomarker and therapeutic target discovery. However, up to now, few proteomes have been identified with the risk of hematologic malignancies. Methods: Genome-wide association studies (GWASs) including 3,083 plasma proteins are based on data from 54,219 people in the UK Biobank Pharma Proteomics Project (UKB-PPP) and 35,559 individuals from Iceland (deCODE). Genetic correlations with 33 hematologic malignancies were derived from the FinnGen cohort and the UK Biobank Data. Further studies, including Bayesian

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… Funding This work was supported by the National Natural Science Foundation of China [grant numbers 82270153, 82270195, and 82070169]; Beijing Municipal General Fiscal Project (2025KT85); the Capital's Funds for Health Improvement and Research (Nos. 2022-1-2152 and 2024-1-2151); Beijing Natural Science … L244063 and L244025); Noncommunicable Chronic Diseases-National Science and Technology Major Project (2023ZD0500800); Beijing Research Ward Excellence Program (BRWEP2024W032150206, BRWEP2024 W032150205, BRWEP2024032150207, BRWEP2024W032150208, BRWEP2024W032150213) and the Peking University Cancer Hospital …

Effectiveness and Safety of Acupuncture for Nausea and Vomiting in Cancer Patients: A Systematic Review and Meta-Analysis.

Background and Objectives: Nausea and vomiting (NV) are common and distressing adverse effects among cancer patients undergoing treatment. Despite the widespread use of pharmacological antiemetics, these medications are often insufficient for controlling nausea and may cause medication interactions and side effects. Acupuncture has been proposed as a complementary therapy; however, the comprehensive analysis of its effects on NV across all emetogenic cancer treatments remains limited. This systematic review and meta-analysis aimed to evaluate the effectiveness and safety of acupuncture in mana

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… Shijitan Hospital, Beijing Friendship Hospital, Beijing Hospital of Traditional Chinese Medicine, and Guang’ Anmen Hospital | All the acupuncturists who participated in this trial were major in acupuncture for more than 3 years with Chinese medicine practitioner license and had undergone rigorous training … in conducting this trial Cheng 2020 [ 28 ] | PC6, ST36 | 4 | NM | 0.25 × 40 mm (Andi med, China) | Manual, De qi (twirling, rotating) | 30 min | 5 | 30 min before or after chemotherapy | Beijing Traditional Chinese Medicine Hospital | Physicians with 3 years of experience performed the acupuncture for …

Clinicopathological Features and Prognostic Differences Between Primary and Relapsed or Refractory Extramedullary Multiple Myeloma.

PURPOSE: This study aims to explore the clinicopathological features related to extramedullary multiple myeloma (EMM) occurrence and evaluate prognosis differences between patients with primary EMM (PEMM) and those with relapsed or refractory EMM (R/REMM). PATIENTS AND METHODS: The laboratory test results, clinical, histopathological and immunophenotypic characteristics, cytogenetic changes and prognosis of 203 patients with MM were obtained. The patients were categorized into EMM+ and EMM- groups. Patients in the EMM+ group were further categorized into the PEMM and R/REMM groups based on th

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… Materials and Methods Case Selection Pathology data were obtained from the Beijing Chao-Yang Hospital, Capital Medical University, between January 2018 and August 2023. The inclusion criteria were as follows: 1. … This study was approved by the institutional review board and ethics committee of Beijing Chao-Yang Hospital and was conducted in accordance with requirements in the Declaration of Helsinki. …

Chimeric antigen receptor T cells targeting CD147 for non-small cell lung cancer therapy.

Non-small cell lung cancer (NSCLC) is a highly malignant tumor, with a significant mortality and morbidity. With the development of tumor immunotherapy, chimeric antigen receptor T cells (CART) gets increasingly attention and achieves prominent contributions in the treatment of hematologic malignancies. However, CART therapy for NSCLC proceeds slowly and further researches need to be investigated. In our study, we performed bioinformatics analysis to evaluate the significant role of CD147 in NSCLC. The expression level of CD147 was detected in human NSCLC cell lines and NSCLC tissues. Meanwhil

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… Meanwhile, the cell lines have been tested and authenticated using Short Tandem Repeat DNA profiling by Beijing Microread Genetics Co., Ltd. (Beijing, China). … For PDX model, the mice were transplanted subcutaneously with NSCLC tumor tissues obtained from BEIJING IDMO Co., Ltd.. …

Research on predicting the progression of multiple myeloma treated with bortezomib based on multimodal ensemble learning.

BackgroundMultiple myeloma (MM) is a malignancy characterized by abnormal plasma cell proliferation. While bortezomib has improved outcomes, significant individual variability persists. Accurate early prediction of patient progression is crucial for optimizing therapeutic intensity and improving long-term survival. Developing an automated, multimodal prediction model can provide clinicians with a robust tool for personalized prognosis, thereby reducing the burden of ineffective treatments on patients.MethodsWe enrolled 207 newly diagnosed MM (NDMM) patients treated with bortezomib. Based on 2-

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… These patients were treated at the Department of Hematology, Beijing Chaoyang Hospital, Capital Medical University, between January 2017 and December 2023. The study was approved by the Ethics Committee of Beijing Chaoyang Hospital, Capital Medical University (Ethics Approval Number: 2024-ke-850). … Yantian Zhao https://orcid.org/0000-0001-7332-5920 Hong Zong https://orcid.org/0009-0002-3953-1776 Hong Huo https://orcid.org/0009-0004-4444-0777 Chuanying Geng https://orcid.org/0000-0001-6080-1983 Ethics approval and informed consent: All procedures involving human participants were approved by the Beijing …

Neutralizing antibody against GDF15 for treatment of cancer-associated cachexia.

GDF15 (growth differentiation factor 15), also known as macrophage inhibitory cytokine 1 (MIC-1), is a circulating protein involved in the regulation of energy balance and weight control. Elevated levels of GDF15 have been associated with cachexia and reduced survival rates in cancer patients. Through the activation of the GFRAL (GDNF-family receptor α-like)-RET (Rearranged during Transfection) signaling pathway, GDF15 can induce weight loss, making it a potential target for treating cachexia. Currently, there are no approved antibody drugs specifically targeting GDF15 for cancer cachexia trea

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… All the mice were housed in specific pathogen-free facilities, and all animal experiments were approved by the Animal Ethics Committee of E-Town Biomedical Park in Beijing, China. … Approval for all animal experiments was obtained from the Animal Ethics Committee of the Beijing E-Town Biomedical Park experimental animal center, Beijing, China. …

Cyclic Polypeptide D7 Protects Bone Marrow Mesenchymal Cells and Promotes Chondrogenesis during Osteonecrosis of the Femoral Head via Growth Differentiation Factor 15-Mediated Redox Signaling.

Osteonecrosis of the femoral head (ONFH) is a debilitating disease that is closely associated with the clinical application of high-dose glucocorticoids. Elevated oxidative stress contributes to the pathophysiological changes observed in ONFH. The lack of effective treatments besides surgical intervention highlights the importance of finding novel therapeutics. Our previous studies demonstrated that D7, a cyclic polypeptide, enhances the adhesion, expansion, and proliferation of bone marrow mesenchymal stem cells (BMSCs). Therefore, in this study, we investigated the therapeutic effects of D7

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… Thereafter, the cells were exposed to Alcian acidizing fluid (Solarbio, Beijing, China) for 3 min and Alcian staining solution for 30 min. 2.12. … C1212; APPLYGEN, Beijing, China) for 10 min and covered with mounting medium, antifading (cat. no. S2100; Solarbio). The slices were sealed and visualized using a fluorescence microscope (Axio Observer 3; Carl Zeiss AG). …

MicroRNA‑145 promotes the apoptosis of leukemic stem cells and enhances drug‑resistant K562/ADM cell sensitivity to adriamycin via the regulation of ABCE1.

Leukemia is a type of cancer which originates in blood‑forming tissues. MicroRNAs (miRNAs or miRs) have been shown to be involved leukemogenesis. In the present study, following the gain‑ and loss‑function of miR‑145 and ATP‑binding cassette sub‑family E member 1 (ABCE1) in K562 cells and K562 adriamycin‑resistant cells (K562/ADM cells), the levels of multidrug resistance protein 1 (MRP1) and P‑glycoprotein (P‑gp) were measured. The viability of the K562 cells and K562/ADM cells treated with various concentrations of ADM, and cell sensitivity to ADM were measured. The apoptosis of stem cells w

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… Following the removal of the supernatant, 150 µ l dimethyl sulphoxide (DMSO) solution (Beijing Solarbio Science & Technology Co., Ltd.) was added to each well, followed by shaking for 10 min at a low speed on a shaking table. … Acridine orange/ethidium bromide (AO/EB) double fluorescence staining K562 cells and K562/ADM cells in each group were mixed with 2 µ l AO/EB working fluid (Beijing Leagene Biotechnology Co., Ltd.) gently in each group. …

USP14 inhibition by degrasyn induces YAP1 degradation and suppresses the progression of radioresistant esophageal cancer.

BACKGROUND: Radiotherapy is a major modality for esophageal cancer (ESCA) treatment, yet radioresistance severely hampers its therapeutic efficacy. Ubiquitin-specific peptidase 14 (USP14) is a novel deubiquitinase and can mediate cancer cells' response to irradiation, although the underlying mechanism remains unclear, including in ESCA. METHODS: To evaluate the expression of USP14 in ESCA tissues or cells, we used RNA-Seq, immunoblotting, co-immunoprecipitation (Co-IP), ubiquitination, quantitative real-time polymerase chain reaction (qRT-PCR), and immunofluorescence assays in this investigat

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… Real time-quantitative PCR (RT-qPCR) analysis Total RNA was extracted from cells and tissue using the RNA Easy Fast Tissue/Cell Kit (TIANGEN, Beijing, China) according to the manufacturer's instructions. … Immunofluorescence (IF) Cells on the coverslips after treatments were fixed by 4 % paraformaldehyde for 15 min, permeabilized using 0.3 % Triton X-100 (Solarbio, Beijing, China) for 10 min, and blocked in 5 % bovine serum albumin (BSA) (Solarbio, Beijing, China) in PBS for 45 min. …

[The development of medieval Salerno Medicine and the standardization of bloodletting therapy].

This paper examines the historical development and medical achievements of the Salerno School of Medicine. The paper focuses on its medical teaching system, explores the promoting effect of anatomical and surgical courses on the clinical practice of bloodletting, analyses the academic thoughts of Salerno physicians and the standardized bloodletting procedures recorded in Regimen Sanitatis Salernitanum, and clarifies the transformation process of Western European bloodletting therapy from empirical operation to standardized practice in the Middle Ages. It was found that the Salerno School of Me